专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明专利

DE602005014025D1 未知
公开(公告)号：DE602005014025D1
公开(公告)日：2009-06-04
申请号：DE602005014025
申请日：2005-02-02
申请人： TOSOH CORP
发明人： MASUDA NORIYOSHI , YASUKAWA KIYOSHI , HORIE RYUICHI
IPC分类号： C12Q1/68
摘要： The present invention provides a detection method for detecting norovirus, using an RNA amplification process comprising the steps of : producing a cDNA with an RNA-dependent DNA polymerase using a specific sequence of norovirus genome RNA as a template, as well as a specifically defined first primer having a sequence homologous to said specific sequence, and a specifically defined second primer having a sequence complementary to said specific sequence, thereby forming a double-strand RNA-DNA, wherein either the first primer or the second primer has a sequence in which a promoter sequence of an RNA polymerase has been added to its 5' end; degrading the RNA portion of said double-strand RNA-DNA by ribonuclease H, thereby producing a single-strand DNA; and producing a double-strand DNA having said promoter sequence capable of transcribing the RNA composed of the specific sequence of the RNA or the sequence complementary to said specific sequence of the RNA with a DNA-dependent DNA polymerase using said single-strand DNA as a template; wherein, the double-strand DNA produces an RNA transcription product in the presence of the RNA polymerase, and said RNA transcription product serves as a template for the subsequent cDNA synthesis with the RNA-dependent DNA polymerase.

2. 发明专利

DE69927375T2 未知
公开(公告)号：DE69927375T2
公开(公告)日：2006-06-22
申请号：DE69927375
申请日：1999-07-01
申请人： TOSOH CORP
发明人： EKIDA TEIJI , YAGAME HARUTAKA , IIDA HIROSHI , YASUKAWA KIYOSHI , TSUCHIYA SHIGEO , IDE TERUHIKO
IPC分类号： C07K19/00 , A61K38/00 , A61K38/20 , C07K14/54 , C07K14/715 , C12N1/19 , C12N5/0789 , C12P21/00

3. 发明专利

DE60109002D1 未知
公开(公告)号：DE60109002D1
公开(公告)日：2005-03-31
申请号：DE60109002
申请日：2001-07-16
申请人： TOSOH CORP
发明人： ISHIGURO TAKAHIKO , YASUKAWA KIYOSHI
IPC分类号： C12N15/09 , C07K1/00 , C12Q1/68
摘要： A novel transcriptome analyzing method, provision of a gene found by this method and a protein encoded by said gene. A method for determining whether or not a continued arbitrary DNA sequence existing in the genome of an arbitrary biological species, of which the nucleotide sequence is already known but its possibility of being an expressed region is unclear (specific region), which comprises detecting whether or not a nucleotide sequence that corresponds to the nucleotide sequence of the region is present in the RNA of the biological species, by means of RT-PCR in combination with NASBA and detection using fluorescent labelled oligonucleotide probes.

4. 发明专利

DE69628878T2 未知
公开(公告)号：DE69628878T2
公开(公告)日：2004-05-27
申请号：DE69628878
申请日：1996-04-17
申请人： TOSOH CORP
发明人： KONDO MASAHIDE , YASUKAWA KIYOSHI , HADA TOSHIKAZU
IPC分类号： C07K16/40 , G01N33/566 , G01N33/573 , G01N33/577 , G01N33/541 , G01N33/576 , G01N33/563
摘要： The object of the present invention is to provide a method for discriminating between liver cirrhosis and chronic hepatitis by serodiagnosis using a simple enzyme immunoassay method without performing histopathological examinations by biopsy and so forth. The present invention discloses an enzyme immunoassay method for cholinesterase using a monoclonal antibody, a method for detecting Aleuria aurantia lectin-reactive cholinesterase using a monoclonal antibody and Aleuria aurantia lectin, and a method for discriminating between liver cirrhosis and chronic hepatitis on the basis of those results.

5. 发明专利

DE60336029D1 Verfahren für den Nachweis säurebeständiger Bakterien mittels ribosomaler RNA als Ziel 未知
公开(公告)号：DE60336029D1
公开(公告)日：2011-03-24
申请号：DE60336029
申请日：2003-12-19
申请人： TOSOH CORP
发明人： MASUDA NORIYOSHI , ISAWA YUICHI , HORIE RYUICHI , YASUKAWA KIYOSHI
IPC分类号： C12Q1/68 , G01N33/53 , C12N15/09 , G01N33/566 , G01N33/569

6. 发明专利

DE69941406D1 未知
公开(公告)号：DE69941406D1
公开(公告)日：2009-10-22
申请号：DE69941406
申请日：1999-07-01
申请人： TOSOH CORP
发明人： EKIDA TEIJI , YAGAME HARUTAKA , IIDA HIROSHI , YASUKAWA KIYOSHI , TSUCHIYA SHIGEO , IDE TERUHIKO
IPC分类号： C07K14/54 , A61K38/00 , A61K38/20 , C07K14/715 , C12N1/19 , C12N5/0789 , C12N15/81

7. 发明专利

DE69319662T2 未知
公开(公告)号：DE69319662T2
公开(公告)日：1998-12-10
申请号：DE69319662
申请日：1993-04-27
申请人： TOSOH CORP , KISHIMOTO TADAMITSU
发明人： KISHIMOTO TADAMITSU , SAITO TAKASHI , SUZUKI HIROSHI , MIKI DAISUKE , YASUKAWA KIYOSHI
IPC分类号： A61K39/395 , A61K38/00 , A61P43/00 , C07K16/28 , C12N5/10 , C12N5/18 , C12N15/02 , C12N15/09 , C12P21/08 , C12R1/91 , G01N33/53
摘要： Monoclonal antibodies recognizing gp130 protein and binding to the protein to inhibit IL-6 functions completely (that is to the same level as that is the absence of IL-6) when present in enough amount; a hybridoma producing the monoclonal antibody; a process for production of the monoclonal antibodies using the hybridoma; and an inhibitory agent for inhibition of physiological actions of IL-6 comprising the monoclonal antibodies.

8. 发明专利

DE602004023462D1 未知
公开(公告)号：DE602004023462D1
公开(公告)日：2009-11-19
申请号：DE602004023462
申请日：2004-08-31
申请人： TOSOH CORP
发明人： MASUDA NORIYOSHI , HORIE RYUICHI , YASUKAWA KIYOSHI
IPC分类号： C12N15/09 , C12Q1/68
摘要： A detection reagent for detecting thermostable direct hemolysin (TDH) gene in an amplification process which specifically amplifies TDH RNA, which reagent comprises a first primer having a sequence complementary to a specific sequence of an RNA derived from the TDH gene, and a second primer having a sequence homologous to said specific sequence.

9. 发明专利

DE602005016313D1 未知
公开(公告)号：DE602005016313D1
公开(公告)日：2009-10-08
申请号：DE602005016313
申请日：2005-02-02
申请人： TOSOH CORP
发明人： MASUDA NORIYOSHI , YASUKAWA KIYOSHI , HORIE RYUICHI
IPC分类号： C12Q1/68
摘要： The present invention provides a detection method for detecting norovirus, using an RNA amplification process comprising the steps of : producing a cDNA with an RNA-dependent DNA polymerase using a specific sequence of norovirus genome RNA as a template, as well as a specifically defined first primer having a sequence homologous to said specific sequence, and a specifically defined second primer having a sequence complementary to said specific sequence, thereby forming a double-strand RNA-DNA, wherein either the first primer or the second primer has a sequence in which a promoter sequence of an RNA polymerase has been added to its 5' end; degrading the RNA portion of said double-strand RNA-DNA by ribonuclease H, thereby producing a single-strand DNA; and producing a double-strand DNA having said promoter sequence capable of transcribing the RNA composed of the specific sequence of the RNA or the sequence complementary to said specific sequence of the RNA with a DNA-dependent DNA polymerase using said single-strand DNA as a template; wherein, the double-strand DNA produces an RNA transcription product in the presence of the RNA polymerase, and said RNA transcription product serves as a template for the subsequent cDNA synthesis with the RNA-dependent DNA polymerase.

10. 发明专利

DE69628878D1 未知
公开(公告)号：DE69628878D1
公开(公告)日：2003-08-07
申请号：DE69628878
申请日：1996-04-17
申请人： TOSOH CORP
发明人： KONDO MASAHIDE , YASUKAWA KIYOSHI , HADA TOSHIKAZU
IPC分类号： C07K16/40 , G01N33/566 , G01N33/573 , G01N33/577 , G01N33/541 , G01N33/576 , G01N33/563
摘要： The object of the present invention is to provide a method for discriminating between liver cirrhosis and chronic hepatitis by serodiagnosis using a simple enzyme immunoassay method without performing histopathological examinations by biopsy and so forth. The present invention discloses an enzyme immunoassay method for cholinesterase using a monoclonal antibody, a method for detecting Aleuria aurantia lectin-reactive cholinesterase using a monoclonal antibody and Aleuria aurantia lectin, and a method for discriminating between liver cirrhosis and chronic hepatitis on the basis of those results.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式