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1. 发明专利

JPH03197865A MEASURING METHOD FOR PHOSPHATIDYLCHOLINE 失效
公开(公告)号：JPH03197865A
公开(公告)日：1991-08-29
申请号：JP33627589
申请日：1989-12-27
申请人： TOSOH CORP
发明人： ABE NAOTO , INOUE KUNIYO
IPC分类号： G01N33/53 , G01N33/577
摘要： PURPOSE:To safely immunologically measure phosphatidylcholine (PC) with a simple operation and high sensitivity in a short period of time by using a monoclonal antibody which specifically recognizes the PC. CONSTITUTION:The PC which is prepd. from the egg yolk, is immobilized on nitrocellulose paper and is homogenated in PBS is directly immunized as an antigen to the spleen of a mouse. The PC formed by coating acid treated Salmonella Minnesota bacteria with the PC is additionally immunized from the vein of the tail part after two weeks. The spleen is extracted from the mouse after 3 days of the final immunization and the antibody forming cells are prepd., by which the hybridoma is prepd. The analysis of the supernatant of the hybridoma culture is then executed and the hybridoma producing the antibody which reacts specifically with the PC is selected. Plural kinds of the monoclonal antibodies which specifically recognize the PC are thus obtd. The independent measurement of the PC in a sample by identifying the same from other phospholipids is, therefore, possible. The measurement of the many specimens with the simple operation and the high sensitivity in a short period of time is thus executed.

2. 发明专利

JPH03160998A MONOCLONAL ANTIBODY AND MEASUREMENT USING SAME ANTIBODY 失效
公开(公告)号：JPH03160998A
公开(公告)日：1991-07-10
申请号：JP29972689
申请日：1989-11-20
申请人： TOSOH CORP
发明人： MORIMOTO KOICHI , KAWAI NOBUYUKI , IBA YOSHITAKA , INOUE KUNIYO
IPC分类号： G01N33/573 , C12N5/10 , C12N5/20 , C12N15/02 , C12N15/06 , C12P21/08 , C12R1/91 , G01N33/577
摘要： PURPOSE:To provide a monoclonal antibody useful for measurement of human alpha1-antichymotrypsin, specifically recognizing human alpha1-antichymotrypsin. CONSTITUTION:A splenocyte of mammal immunized against human alpha1- antichymotrypsin and a myeloma cell are subjected to cell fusion to prepare a hybridoma and a monoclonal antibody produced by the prepared hbridoma is collected to give a monoclonal antibody specifically recognizing human alpha1- antichymotrypsin. The immobilized monoclonal antibody is brought into contact with a labeled monoclonal antibody to specifically recognizing human alpha1- antichymotrypsin at a site different from that of the immobilized monoclonal antibody and a specimen. Then the free antibody and the specimen are removed, the label is detected and formed immunological reaction product is determined to measure human alpha1-antichymotrypsin.

3. 发明专利

JPH02152932A ANTIVIRAL DRUG AND PRODUCTION THEREOF 失效
公开(公告)号：JPH02152932A
公开(公告)日：1990-06-12
申请号：JP21058988
申请日：1988-08-26
申请人： TOSOH CORP
发明人： INOUE KUNIYO , KYODA TAKAHIRO , NAKAI MASUYO
IPC分类号： A61K39/395 , A61P35/00 , C07K1/113 , C07K14/00 , C07K14/76 , C07K16/00 , C07K19/00
摘要： NEW MATERIAL:A compound shown by formula I (A is monoclonal antibody against human T cell leukemia virus-III; B is human derived soluble protein; m and n are positive integer). USE:An agent for missile therapy for AIDS. PREPARATION:3'-Deoxy-3'-azidothymidine derivative shown by formula II is bonded to a human derived soluble protein by reacting free amino group of the protein with aldehyde group of the compound shown by formula II, treated with a mild reducing agent and a monoclonal antibody is bonded to the human derived soluble protein by using carbodiimide to give the aimed substance.

4. 发明专利

JPH0259526A AZIDOTHYMIDINE-PROTEIN COMPLEX AND PRODUCTION THEREOF 失效
公开(公告)号：JPH0259526A
公开(公告)日：1990-02-28
申请号：JP21058888
申请日：1988-08-26
申请人： TOSOH CORP
发明人： INOUE KUNIYO , KYODA TAKAHIRO , NAKAI MASUYO
IPC分类号： A61K38/00 , A61K39/395 , A61P31/12 , C07K1/113 , C07K14/00 , C07K14/76 , C07K16/00
摘要： PURPOSE:To obtain an azidothymidine-protein complex bonded by a novel bond site. CONSTITUTION:CHO at the 5'-position of 3'-deoxy-3'-azidothymidine(AZT) shown by formula I is reacted with amino group of protein to form a Schiff base, which is then treated with a mild reducing agent to give AZT-protein to give AZT-protein complex shown by formula II. Monoclonal antibody, polyclonal antibody of mouse, human, rat, etc., serum albumin, lysozyme, fibrin, etc., of various animals may be cited as the protein. When an antiviral antibody is used as the protein, the complex is expected to have effects as missile therapy having antiviral action.

5. 发明专利

JPH01302162A MEASUREMENT OF HUMAN MIOGLOBIN 失效
公开(公告)号：JPH01302162A
公开(公告)日：1989-12-06
申请号：JP13168988
申请日：1988-05-31
申请人： TOSOH CORP
发明人： MORIMOTO KOICHI , KAWAI NOBUYUKI , INOUE KUNIYO
IPC分类号： G01N33/53 , C12N15/00 , C12N15/02 , C12P21/00 , C12P21/08 , G01N33/577
摘要： PURPOSE:To enable highly accurate measurement of human mioglobin with a handy operation, by bringing a monoclonal antibody immobilized for recognizing the human mioglobin specifically into contact with a sample. CONSTITUTION:Monoclonal antibody 1 immobilized for recognizing human mioglobin specifically is brought into contact with a sample and an immunoreaction product generated is brought into contact with a monoclonal antibody 2 for recognizing human mioglobin at a part different from the monoclonal antibody 1. When a monoclonal antibody not labeled is used, the immunoreaction product is brought into contact with an antibody labeled for recognizing the monoclonal antikbody 2 specifically. Then, an antibody labeled for separation is removed to assay the labeled antibody, and the generated immunoreaction product is subjected to quantitative determination.

6. 发明专利

JPS6459068A METHOD FOR MEASURING HUMAN THYROXIN BINDING GLOBULIN 失效
公开(公告)号：JPS6459068A
公开(公告)日：1989-03-06
申请号：JP21507387
申请日：1987-08-31
申请人： TOSOH CORP
发明人： INOUE KUNIYO , HORIE MISAO
IPC分类号： G01N33/577 , G01N33/53 , G01N33/543
摘要： PURPOSE:To widen a linear area when a calibration curve is made by adopting a 2 monoclonal antibody sandwich method using an immobilized 1st antibody and 2nd antibody which is labeled by a labeling agent. CONSTITUTION:The 1st antibody which is the monoclonal antibody to recognize TBG and is immobilized and the 2nd antibody which recognizes the antigen section different from the 1st antibody and is labeled by the labeling agent are used in the method of measuring the human thyroxin binding globulin TBG in a sample by an immunological method. The monoclonal antibody is preferably obtd. by fusing the antibody genic cells of an animal immunized by TBG and myeloma cells to obtain the hybridoma which produces the monoclonal antibody to recognize TBG, then culturing this hybridoma or the cell strains derived therefrom and drawing the monoclonal antibody from the cultured matter thereof. The solid phase immune enzyme immunoassay by the sandwich method using the two antibodies is, therefore, enabled. The conjugation constant of the 1st and 2nd antibodies is preferably 10 -10 l/mol.

7. 发明专利

JPH11174038A SEPARATING AND REFINING METHOD FOR PROTEIN 失效
公开(公告)号：JPH11174038A
公开(公告)日：1999-07-02
申请号：JP33977097
申请日：1997-12-10
申请人： TOSOH CORP
发明人： MORIMOTO KOICHI , INOUE KUNIYO
IPC分类号： G01N33/68 , B01D15/08 , B01J20/281 , G01N30/26 , G01N30/88 , G01N30/48
摘要： PROBLEM TO BE SOLVED: To provide an efficiently separating and refining method of protein by a hydrophobic interaction chromatography without causing an adsorption of the protein to be separated and refined to a carrier. SOLUTION: In a protein separating and refining method by a hydrophobic interaction liquid chromatography, a hydrophobic interaction liquid chromatography is performed under the condition that the protein is dissolved in a buffer solution containing an organic solvent within the range of concentration that the protein to be separated and refined is not denatured irreversibly.

8. 发明专利

JPH03200799A METHOD FOR SEPARATING ANTIBODY 失效
公开(公告)号：JPH03200799A
公开(公告)日：1991-09-02
申请号：JP33810489
申请日：1989-12-28
申请人： TOSOH CORP
发明人： ABE NAOTO , INOUE KUNIYO
IPC分类号： G01N33/577 , A61K39/395 , B01D15/08 , C07K1/20 , C07K16/00 , C12P21/08
摘要： PURPOSE:To efficiently obtain the subject respective antibodies in high purity by bringing a sample solution containing plural kinds of monoclonal antibodies having protein chemically different H- and L-chains into contact with a specific resin, adsorbing the antibodies thereon and selectively separating the antibodies. CONSTITUTION:For example, a monoclonal antibody-containing sample solution such as an ascitic fluid of an animal to which a hybridoma is transplanted is initially salted out with ammonium sulfate, etc., to highly purity antibodies by ion exchange chromatography. The resultant antibody fraction is precipitated and concentrated with a 50% ammonium sulfate solution and further solubilized in a

9. 发明专利

JPH03200066A METHOD FOR MEASURING ACTIVATED HUMAN PROTEIN C 失效
公开(公告)号：JPH03200066A
公开(公告)日：1991-09-02
申请号：JP33810589
申请日：1989-12-28
申请人： TOSOH CORP
发明人： KYODA TAKAHIRO , MAKI KOJI , INOUE KUNIYO
IPC分类号： G01N33/577
摘要： PURPOSE:To exactly and immunologically measure the human protein C in blood in a short period of time with a high sensitivity by utilizing the monoclonal antibody specific for activated human protein C and human protein C inhibitor, etc. CONSTITUTION:The monoclonal antibody immobilized to the solid phase which specifically recognizes the activated human protein C, a sample, the monoclonal antibody labeled to specifically recognized the human protein C inhibitor, and the monoclonal antibody labeled to specifically recognize human a1 anti-trypsin are brought into contact and the label of the liberated or immobilizd labeled antibody is directly or indirectly detected to determine the product of immune reaction. A sandwich method is used for this immunoassay. Any known methods may be adopted without limitations for the process for producing the monoclonal antibodies, the method for immobilizing the solid phase of the activated human protein C antibody, the method for labeling the human protein C inhibitor antibody, etc.

10. 发明专利

JPH03183953A METHOD FOR MEASURING HUMAN PANCREAS ALPHA-AMYLASE 失效
公开(公告)号：JPH03183953A
公开(公告)日：1991-08-09
申请号：JP32153289
申请日：1989-12-13
申请人： TOSOH CORP
发明人： IBA YOSHITAKA , MORIMOTO KOICHI , KAWAI NOBUYUKI , INOUE KUNIYO
IPC分类号： G01N33/573 , G01N33/577
摘要： PURPOSE:To immunologically measure the human pancreas alpha-amylase with high accuracy and high sensitivity in a wide concn. range with a simple operation in a short period of time by using a monoclonal antibody specific to the human pancreas alpha-amylase. CONSTITUTION:The human pancreas alpha-amylase is immunologically measured by a sandwich method using the 1st antibody A immobilized by the monoclonal antibody which specifically recognizes the human pancreas alpha-amylase and does not make cross reaction with the human salivary gland alpha-amylase and the 2nd antibody B which recognizes the antigen section different form the antibody A and is labeled by a labeling agent. The antibodies may be immobilized by any known method. The sample is not restricted at all and any known method may be used for the labeling of the antibodies. There are no restrictions on order of adding the sample, the antibody A and the antibody B in the sandwich measuring method and either method of 1-step sandwich and 2-step sandwich may be used. Washing, etc., of a B/F sepn. method, etc., to be used in ordinary immunoassay may be executed to remove the free labeled antibodies.

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