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1. 发明申请

WO2012170355A2 POTATO TRANSFORMATION COMPOSITIONS, SYSTEMS, METHODS, MICROORGANISMS, AND PLANTS 审中-公开
标题翻译： POTATO转化组合物，系统，方法，微生物和植物
公开(公告)号：WO2012170355A2
公开(公告)日：2012-12-13
申请号：PCT/US2012/040769
申请日：2012-06-04
申请人： THE TEXAS A&M UNIVERSITY SYSTEM , CURTIS, Ian S. , GONZALEZ, Javier , MIRKOV, T. Erik
发明人： CURTIS, Ian S. , GONZALEZ, Javier , MIRKOV, T. Erik
IPC分类号： A23L19/12 , A23L19/15 , A23L19/18 , A23L23/00
CPC分类号： C12N15/8205 , C12N15/8281
摘要： The present disclosure relates, in some embodiments, to potato transformation compositions, systems, methods, microorganisms, and plants (e.g., one or more potato chipping varieties). In some embodiments, a method of transforming and/or transfecting a plant (e.g., 'Atlantic' potato) may comprise (a) growing an 'Atlantic' potato plant (e.g., from a tuber) for from about 3 weeks to about 4 weeks, (b) removing one or more leaf sections (e.g., each section from about 0.5 cm to about 1 cm in its longest dimension) from the plant., (c) cultivating the one or more sections on a callus induction medium comprising zeatin for about 2 days, and/or (d) contacting the one or more sections with Agrobacterium comprising the exogenous nucleic acids under conditions that permit transfer of the exogenous nucleic acid to the one or more sections to produce at least one transformed and/or transfected plant cell.
摘要翻译：在一些实施方案中，本公开涉及马铃薯转化组合物，系统，方法，微生物和植物（例如，一种或多种马铃薯切片品种）。在一些实施方案中，转化和/或转染植物（例如，“大西洋”马铃薯）的方法可以包括（a）将“大西洋”马铃薯植物（例如，从块茎）生长约3周至约4周，（b）从植物中除去一个或多个叶片部分（例如，每个部分在其最长尺寸上从约0.5cm至约1cm），（c）在包含玉米素的愈伤组织诱导培养基上培养一个或多个切片，约2天，和/或（d）在允许外源核酸转移到一个或多个部分以产生至少一个转化和/或转染的植物的条件下，使一个或多个切片与包含外源核酸的农杆菌接触细胞。

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