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1. 发明申请

WO2012058686A3 HYBRID POLYKETIDE SYNTHASES 审中-公开
公开(公告)号：WO2012058686A3
公开(公告)日：2012-05-03
申请号：PCT/US2011/058660
申请日：2011-10-31
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA , FORTMAN, Jeffrey L. , HAGEN, Andrew , KATZ, Leonard , KEASLING, Jay , POUST, Sean , ZHANG, Jingwei , ZOTCHEV, Sergey
发明人： FORTMAN, Jeffrey L. , HAGEN, Andrew , KATZ, Leonard , KEASLING, Jay , POUST, Sean , ZHANG, Jingwei , ZOTCHEV, Sergey
IPC分类号： C12P5/02
摘要： The present invention provides for a polyketide synthase (PKS) capable of synthesizing an even-chain or odd-chain diacid or lactam or diamine. The present invention also provides for a host cell comprising the PKS and when cultured produces the even-chain diacid, odd-chain diacid, or KAPA. The present invention also provides for a host cell comprising the PKS capable of synthesizing a pimelic acid or KAPA, and when cultured produces biotin.

2. 发明申请

WO2014093402A2 A SYNTHETIC TRANSCRIPTION FACTOR AND USES THEREOF 审中-公开
标题翻译：合成转录因子及其用途
公开(公告)号：WO2014093402A2
公开(公告)日：2014-06-19
申请号：PCT/US2013/074214
申请日：2013-12-10
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA , CHOU, Howard, H. , KEASLING, Jay, D. , ZOTCHEV, Sergey
发明人： CHOU, Howard, H. , KEASLING, Jay, D. , ZOTCHEV, Sergey
IPC分类号： C12N15/04
CPC分类号： C07K14/4702 , C07K2319/00 , C07K2319/70 , C07K2319/80
摘要： The present invention provides for a synthetic transcription factor (TF) comprising a first peptide capable of binding a target ligand, a second peptide capable of binding a target DNA, and a peptide linker linking the first and second peptides. The present invention also provide for a system for modulating the mutagenesis frequency of a host cell. The host cell has a mutator rate (R) which is inversely proportional to a phenotypic trait (P).
摘要翻译：本发明提供了包含能够结合靶配体的第一肽，能够结合靶DNA的第二肽和连接第一和第二肽的肽接头的合成转录因子（TF）。本发明还提供了用于调节宿主细胞的诱变频率的系统。宿主细胞具有与表型性状（P）成反比的突变率（R）。

3. 发明申请

WO2009115822A1 NRPS-PKS GENE CLUSTER AND ITS MANIPULATION AND UTILITY 审中-公开
标题翻译： NRPS-PKS基因簇及其操纵和实现
公开(公告)号：WO2009115822A1
公开(公告)日：2009-09-24
申请号：PCT/GB2009/000759
申请日：2009-03-20
申请人： SINVENT AS , ZOTCHEV, Sergey , JØRGENSEN, Hanne , SLETTA, Havard , ELLINGSEN, Trond, Erling , FJAERVIK, Espen , DEGNES, Kristin, Fløgstad , KLINKENBERG, Geir , BRUHEIM, Per , DZIEGLEWSKA, Hanna Eva
发明人： ZOTCHEV, Sergey , JØRGENSEN, Hanne , SLETTA, Havard , ELLINGSEN, Trond, Erling , FJAERVIK, Espen , DEGNES, Kristin, Fløgstad , KLINKENBERG, Geir , BRUHEIM, Per
IPC分类号： C07K14/36 , C12N15/52 , C12N1/20 , C07G11/00
CPC分类号： C12N15/52 , C07D225/02 , C07K14/36 , C12P17/10 , C12P19/62 , C12R1/465
摘要： The present invention provides a nucleic acid molecule comprising: (a) a nucleotide sequence as shown in SEQ ID No. 1; or (b) a nucleotide sequence which is the complement of SEQ ID No. 1; or (c) a nucleotide sequence which is degenerate with SEQ ID No. 1; or (d) a nucleotide sequence having at least 85% sequence identity (preferably at least 87%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity) with SEQ ID No. 1; or (e) a part of any one of (a) to (d), wherein said nucleic acid molecule encodes or is a complementary to a nucleic acid molecule encoding one or more polypeptides, or comprises or is complementary to a nucleic acid molecule comprising one or more genetic elements, having functional activity in the synthesis of a polyketide-based or macrolactam molecule. Particularly the invention contemplates the modification of the nucleic acid of the invention, encoding the biosynthetic machinery for the synthesis of the polyketide macrolactam BE- 14106, including expressing in a microorganism the modified nucleic acid molecule. In certain aspects the modification includes introducing, mutating, deleting, replacing or inactivating a sequence encoding one or more activities or proteins encoded by said nucleic acid molecule. Other aspects of the invention include a microorganism containing the modified and unmodified nucleic acid and recovering the polyketide-based or macrolactam molecule from said mircoorganism.
摘要翻译：本发明提供了一种核酸分子，其包含：（a）SEQ ID No.1所示的核苷酸序列; 或（b）作为SEQ ID No.1的互补序列的核苷酸序列; 或（c）与SEQ ID No.1退行的核苷酸序列; 或（d）具有至少85％序列同一性（优选至少87％，90％，91％，92％，93％，94％，95％，96％，97％，98％或99％序列同一性）; 或（e）（a）至（d）中任一项的一部分，其中所述核酸分子编码或与编码一种或多种多肽的核酸分子互补，或包含或互补于包含一种或多种在合成聚酮化合物或大内酰胺分子中具有功能活性的遗传元件。特别地，本发明考虑了本发明的核酸的修饰，其编码用于合成聚酮化合物大内酰胺BE-14106的生物合成机理，包括在微生物中表达经修饰的核酸分子。在某些方面，修饰包括引入，突变，缺失，替换或失活编码由所述核酸分子编码的一种或多种活性或蛋白质的序列。本发明的其它方面包括含有经修饰和未修饰的核酸的微生物，并从所述微生物中回收聚酮化合物或大环内酰胺分子。

4. 发明申请

WO2007148121A2 NUCLEIC ACID MOLECULES 审中-公开
标题翻译：核酸分子
公开(公告)号：WO2007148121A2
公开(公告)日：2007-12-27
申请号：PCT/GB2007/002357
申请日：2007-06-25
申请人： STATOIL ASA , LEATHLEY, Anna , KOTLAR, Hans, Kristian , ELLINGSEN, Trond , ZOTCHEV, Sergey , WENTZEL, Alexander , THRONE-HOLST, Mimmi
发明人： KOTLAR, Hans, Kristian , ELLINGSEN, Trond , ZOTCHEV, Sergey , WENTZEL, Alexander , THRONE-HOLST, Mimmi
IPC分类号： C12N15/53 , C12N9/02 , C12N1/21 , C10G32/00
CPC分类号： C07K14/212 , C12N9/0071
摘要： Provided is a nucleic acid molecule comprising the sequence is set forth in SEQ ID NO:1, the corresponding protein sequence as set forth in SEQ ID NO:2, and variants and functional fragments thereof. Corresponding chimeric genes, vectors and microorganisms are also provided. The protein has a role in the degradation of long-chain n -alkanes and can be used to modify the properties of long-chain n - alkane containing substrates. Methods of using the protein of the invention, e.g. to cause degradation of long-chain n -alkanes are also provided, as are methods of identifying enzymes that are required for the metabolism of a substrate.
摘要翻译：提供了包含SEQ ID NO：1所示序列，SEQ ID NO：2所示的相应蛋白质序列的核酸分子及其变体和功能片段。还提供了相应的嵌合基因，载体和微生物。该蛋白质在长链正构烷烃的降解中具有作用，可用于改变含有长链烷烃的底物的性质。使用本发明的蛋白质的方法，例如，还提供了长链正构烷烃的降解，以及鉴定底物代谢所需的酶的方法。

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