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1. 发明申请

WO2009108774A2 USE OF SYNTHETIC SCAFFOLDS FOR THE PRODUCTION OF BIOSYNTHETIC PATHWAY PRODUCTS 审中-公开
标题翻译：使用合成螯合物生产生物路线产品
公开(公告)号：WO2009108774A2
公开(公告)日：2009-09-03
申请号：PCT/US2009/035274
申请日：2009-02-26
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA , DUEBER, John E. , KEASLING, Jay D. , WU, Gabriel C. , MALMIRCHEGINI, Ghulam Reza Khan
发明人： DUEBER, John E. , KEASLING, Jay D. , WU, Gabriel C. , MALMIRCHEGINI, Ghulam Reza Khan
IPC分类号： C12N15/63 , C12N15/11 , C07K14/00
CPC分类号： C12N9/00 , C07K2/00 , C07K2319/00 , C12N15/1044 , C12N15/52 , C12N15/62 , C12N15/63
摘要： The present invention provides methods of producing a product or product precursor of a biosynthetic pathway in a genetically modified host cell. The present invention also provides genetically modified host cells comprising nucleic acids encoding a scaffold polypeptide and nucleic acids comprising nucleotide sequences encoding two or more enzymes in a biosynthetic pathway. The present invention further provides nucleic acids comprising nucleotide sequences encoding scaffold polypeptides, for use in a subject method.
摘要翻译：本发明提供了在遗传修饰的宿主细胞中生产生物合成途径的产物或产物前体的方法。本发明还提供包含编码支架多肽的核酸和包含编码生物合成途径中的两种或更多种酶的核苷酸序列的核酸的遗传修饰的宿主细胞。本发明进一步提供核酸，其包含编码支架多肽的核苷酸序列，用于本发明方法。

2. 发明申请

WO2016049364A2 TYROSINE HYDROXYLASE VARIANTS AND METHODS OF USE THEREOF 审中-公开
标题翻译：酪氨酸羟化酶变体及其使用方法
公开(公告)号：WO2016049364A2
公开(公告)日：2016-03-31
申请号：PCT/US2015/052040
申请日：2015-09-24
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA , MARTIN, Vincent J.J. , NARCROSS, Lauren
发明人： MARTIN, Vincent J.J. , NARCROSS, Lauren , DUEBER, John E. , DELOACHE, William C. , RUSS, Zachary N.
IPC分类号： C12P13/22 , C12N9/06
CPC分类号： C12N9/0071 , C12P7/24 , C12P13/001 , C12P13/225 , C12P17/10 , C12Q1/26 , C12Y114/16002
摘要： The present disclosure provides a variant tyrosine hydroxylase that provides for increased production of L-DOPA in a host cell that expresses the tyrosine hydroxylase. The present disclosure provides nucleic acids encoding the variant tyrosine hydroxylase, and host cells genetically modified with the nucleic acids. The present disclosure provides methods of making L-DOPA in a host cell. The present disclosure provides methods of making a benzylisoquinoline alkaloid (BIA), or a BIA precursor. The present disclosure provides methods of detecting L-DOPA level in a cell. The present disclosure provides methods of identifying tyrosine hydroxylase variants that provide for increased L-DOPA production; and methods of identifying gene products that provide for increased tyrosine production.
摘要翻译：本公开提供了一种变体酪氨酸羟化酶，其提供表达酪氨酸羟化酶的宿主细胞中L-DOPA的增加的产生。本公开提供编码变体酪氨酸羟化酶的核酸和用核酸遗传修饰的宿主细胞。本公开提供了在宿主细胞中制备L-DOPA的方法。本公开提供制备苄基异喹啉生物碱（BIA）或BIA前体的方法。本公开提供了检测细胞中的L-DOPA水平的方法。本公开提供了鉴定提供增加的L-DOPA产生的酪氨酸羟化酶变体的方法; 以及鉴定提供增加酪氨酸生产的基因产物的方法。

3. 发明申请

WO2018067458A1 ENGINEERED MICROORGANISMS FOR PRODUCTION OF COMMODITY CHEMICALS AND CELLULAR BIOMASS 审中-公开
标题翻译：用于生产商品化学品和细胞生物质的工程微生物
公开(公告)号：WO2018067458A1
公开(公告)日：2018-04-12
申请号：PCT/US2017/054759
申请日：2017-10-02
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
发明人： DUEBER, John E. , PROTZKO, Ryan Joseph
IPC分类号： C12N9/00 , C12N9/04 , C12N15/53 , C12N15/00 , C12P7/58 , C12P7/44
摘要： The present disclosure provides methods of producing commodity products, the methods involving culturing a host cell that is genetically modified to produce a uronate dehydrogenase (UDH) that converts a sugar acid to its corresponding 1,5-aldonolactone, that uses NADP + or NAD + as a cofactor, and that produces NADPH or NADH, respectively, where the host cell coexpresses an endogenous or a heterologous reductase that utilizes the produced NADPH or NADH to generate the commodity product or a precursor thereof. The present disclosure provides a method of producing downstream products of glycerol and pyruvate in a genetically modified microbial host cell, the method involving culturing a genetically modified microbial host cell of the present disclosure in a culture medium comprising D-galacturonic acid. The present disclosure provides variant UDH polypeptides that utilize NADP + , nucleic acids encoding the variant UDH polypeptides; and host cells genetically modified with the nucleic acids.
摘要翻译：本发明提供了生产商品的方法，所述方法包括培养经遗传修饰的宿主细胞以产生糖醛酸脱氢酶（UDH），所述糖醛酸脱氢酶将糖酸转化为其相应的1,5-醛糖内酯，其使用NADP +或NAD +作为辅因子，并且分别产生NADPH或NADH，其中宿主细胞共表达利用产生的内源或异源还原酶 NADPH或NADH以产生商品产品或其前体。本公开内容提供了一种在遗传修饰的微生物宿主细胞中生产甘油和丙酮酸的下游产物的方法，所述方法包括在包含D-半乳糖醛酸的培养基中培养本公开的遗传修饰的微生物宿主细胞。本公开提供了利用NADP +，编码变体UDH多肽的核酸的变体UDH多肽; 和用核酸遗传修饰的宿主细胞。

4. 发明公开

EP2255005A2 USE OF SYNTHETIC SCAFFOLDS FOR THE PRODUCTION OF BIOSYNTHETIC PATHWAY PRODUCTS 审中-公开
标题翻译：合成支架用于生产BIOSYNTHESEWEGPRODUKTEN使用
公开(公告)号：EP2255005A2
公开(公告)日：2010-12-01
申请号：EP09714863.9
申请日：2009-02-26
申请人： The Regents of the University of California
发明人： DUEBER, John E. , KEASLING, Jay D. , WU, Gabriel C. , MALMIRCHEGINI, Ghulam Reza Khan
IPC分类号： C12N15/63 , C12N15/11 , C07K14/00
CPC分类号： C12N9/00 , C07K2/00 , C07K2319/00 , C12N15/1044 , C12N15/52 , C12N15/62 , C12N15/63
摘要： The present invention provides methods of producing a product or product precursor of a biosynthetic pathway in a genetically modified host cell. The present invention also provides genetically modified host cells comprising nucleic acids encoding a scaffold polypeptide and nucleic acids comprising nucleotide sequences encoding two or more enzymes in a biosynthetic pathway. The present invention further provides nucleic acids comprising nucleotide sequences encoding scaffold polypeptides, for use in a subject method.

5. 发明公开

EP3519567A1 ENGINEERED MICROORGANISMS FOR PRODUCTION OF COMMODITY CHEMICALS AND CELLULAR BIOMASS 无效
公开(公告)号：EP3519567A1
公开(公告)日：2019-08-07
申请号：EP17858964.4
申请日：2017-10-02
申请人： The Regents of the University of California
发明人： DUEBER, John E. , PROTZKO, Ryan Joseph
IPC分类号： C12N9/00 , C12N9/04 , C12N15/53 , C12N15/00 , C12P7/58 , C12P7/44

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