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1. 发明公开

EP1652918A3 Methods for detecting protein interactions using conformationally constrained peptides 失效
标题翻译：一种用于基于与有限的构象的肽检测蛋白质相互作用的方法
公开(公告)号：EP1652918A3
公开(公告)日：2006-08-16
申请号：EP05020780.2
申请日：1997-04-09
申请人： THE GENERAL HOSPITAL CORPORATION , Genetics Institute, LLC
发明人： Brent, Roger , McCoy, John M. , Jessen, Timm H. , Xu, Chanxing Wilson
IPC分类号： G01N33/68 , G01N33/50 , C12N15/10 , C12Q1/68 , C07K19/00 , C07K7/00
CPC分类号： C12Q1/025 , C07K7/08 , C07K14/00 , C07K2319/00 , C12N9/0036 , C12N15/1055 , C12Q1/6897 , C40B30/04 , G01N33/68 , G01N33/6845
摘要： The invention concerns a method for detecting a protein of interest in a sample, comprising :

(a) contacting said sample with a conformationally constrained protein which is capable of specifically binding to said protein and forming a complex; and
(b) detecting said complex.

2. 发明公开

EP1405911A1 Interaction trap systems for detecting protein interactions 失效
标题翻译：系统zur Erkennung von Protein-Interaktionen
公开(公告)号：EP1405911A1
公开(公告)日：2004-04-07
申请号：EP03021647.7
申请日：1995-07-20
申请人： THE GENERAL HOSPITAL CORPORATION , Genetics Institute, LLC
发明人： Brent, Roger, Dr. , McCoy, John M. , Jessen, Timm H.
IPC分类号： C12N15/11 , C12N15/62 , C07K14/00 , C12N9/02
CPC分类号： C12N9/0036 , C07K2319/00 , C07K2319/35 , C07K2319/70 , C07K2319/71 , C07K2319/80 , C12N15/1055 , C12Q1/6897
摘要： Disclosed herein is a method of determining whether a first protein is capable of physically interacting with a second protein, involving: (a) providing a host cell which contains (i) a reporter gene operably linked to a protein binding site; (ii) a first fusion gene which expresses a first fusion protein, the first fusion protein including the first protein covalently bonded to a binding moiety which is capable of specifically biding to the protein binding site; and (iii) a second fusion gene which expresses a second fusion protein, the second fusion protein including the second protein covalently bonded to a gene activating moiety and being conformationally-constrained; and (b) measuring expression of the reporter gene as a measure of an interaction between the first and the second proteins. Also disclosed are methods for assaying protein interactions, and identifying antagonists and agonists of protein interactions. Proteins isolated by these methods are also discussed. Finally, populations of eukaryotic cells are disclose, each cell having a recombinant DNA molecule encoding a conformationally-constrained intracellular peptide.
摘要翻译：确定第一蛋白质（A）是否能够与第二蛋白质（B）物理相互作用包括提供宿主细胞，其包含：（i）与DNA结合蛋白识别位点可操作地连接的报告基因; （ii）表达（A）的第一融合基因，其包含与能够特异性结合DNA结合蛋白识别位点的结合部分共价结合的第一蛋白质; （iii）表达（B）的第二融合基因，其包含与基因激活部分共价结合并被构象约束的第二蛋白，并测量报告基因的表达作为（A）和（ B）。相同的系统应用于：（i）检测蛋白质群体中的相互作用蛋白质; （ii）识别候选交互者; 和（iii）测定（A）和（B）之间的相互作用。

3. 发明公开

EP1268834A1 SECRETED PROTEINS AND POLYNUCLEOTIDES ENCODING THEM 审中-公开
标题翻译：分泌蛋白确保两编码多核苷酸
公开(公告)号：EP1268834A1
公开(公告)日：2003-01-02
申请号：EP01964686.8
申请日：2001-03-30
申请人： Genetics Institute, LLC
发明人： Jacobs, Kenneth , McCoy, John M. , Lavallie, Edward R. , Collins-Racie, Lisa A. , Evans, Cheryl , Merberg, David , Treacy, Maurice , Agostino, Michael J. , Bowman, Michael R. , Spaulding, Vikki , Wong, Gordon G. , Clark, Hilary F. , Fechtel, Kim , Howes, Steven H. , Resnick, Richard J. , Gulukota, Kamalakar , Graham, James R.
IPC分类号： C12N15/86 , C12P21/06 , C07H21/04
CPC分类号： C07K14/47
摘要： Novel polynucleotides and the proteins encoded thereby are disclosed.

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