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1. 发明授权

US11994508B2 Method and system for linearization and translocation of single protein molecules through nanopores 有权
公开(公告)号：US11994508B2
公开(公告)日：2024-05-28
申请号：US17645943
申请日：2021-12-23
申请人： Northeastern University , The Board of Trustees of the University of Illinois
发明人： Meni Wanunu , Luning Yu , Aleksei Aksimentiev , Behzad Mehrafrooz
IPC分类号： G01N33/487 , B01L3/00 , C12Q1/34 , G01N33/68
CPC分类号： G01N33/48721 , B01L3/502715 , B01L3/502761 , C12Q1/34 , G01N33/6818 , B01L2200/0663 , B01L2300/0645 , B01L2300/0663 , B01L2400/0418
摘要： A method and system for performing single molecule proteomics utilizing a nanopore sensor to measure an electronic signature of protein or peptide being transported through the nanopore from a first chamber to a second chamber. The protein's electronic signature is a function of ionic current over time. The method and system utilizing an agent, such as guanidinium chloride, to bind to the nanopore's interior and provide an electroosmotic force within the nanopore. The electroosmotic force, in some embodiments, enables stretching and unfolding of the protein during transport through the nanopore. The agent may also or alternatively induce the unfolding of the protein before transport through the nanopore and/or provide force moving the protein through the nanopore.

2. 发明授权

US09638661B2 Method and apparatus for controlling a flow of particles by way of a through-hole 有权
公开(公告)号：US09638661B2
公开(公告)日：2017-05-02
申请号：US13773863
申请日：2013-02-22
申请人： THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS
发明人： Aleksei Aksimentiev
IPC分类号： G01N27/447 , G01N33/487 , G01N15/14
CPC分类号： G01N27/447 , G01N15/1404 , G01N27/44791 , G01N33/48721 , G01N2015/1422 , Y10T137/7722
摘要： A system that incorporates the subject disclosure may include, for example, a method for generating an electric or pressure difference force that induces a plurality of particles to flow through a through-hole. Independently adjustable heat source in a vicinity of the through-hole induces a thermodynamic force for modifying the flow of the plurality of particles through the through-hole. Additional embodiments are disclosed.

3. 发明授权

US11441118B2 Nucleic acid and other compositions and methods for the modulation of cell membranes 有权
公开(公告)号：US11441118B2
公开(公告)日：2022-09-13
申请号：US16179214
申请日：2018-11-02
申请人： The Board of Trustees of the University of Illinois , Cambridge Enterprise Limited
发明人： Aleksei Aksimentiev , Ulrich F. Keyser
IPC分类号： C12N15/11 , A61K31/711 , A61K31/713 , C12N5/00 , A61K9/51
摘要： The present invention provides compositions and methods for transferring phospholipids and other molecules between the leaflets of a cell membrane. The compositions comprise at least one nucleic acid or compound having a hydrophilic region, where the composition is able to form a nanostructure that forms a toroidal pore in a lipid membrane. The nucleic acid or hydrophilic region-containing compound further contains an attached molecule capable of inserting the nanostructure into the lipid membrane. The invention also provides methods for scrambling lipids and other molecules in a cell membrane, which can be used to alter the function of a selected cell or to facilitate the death of the cell. The scrambling activity of synthetic scramblases described herein outperforms previously known enzymatically active DNA nanostructures and naturally occurring scramblases, in some cases by several orders of magnitude.

4. 发明申请

US20130220811A1 METHOD AND APPARATUS FOR CONTROLLING A FLOW OF PARTICLES BY WAY OF A THROUGH-HOLE 有权
标题翻译：用于通过孔的方式控制颗粒流的方法和装置
公开(公告)号：US20130220811A1
公开(公告)日：2013-08-29
申请号：US13773863
申请日：2013-02-22
申请人： The Board of Trustees of The University of Illinois
发明人： Aleksei Aksimentiev
IPC分类号： G01N27/447
CPC分类号： G01N27/447 , G01N15/1404 , G01N27/44791 , G01N33/48721 , G01N2015/1422 , Y10T137/7722
摘要： A system that incorporates the subject disclosure may include, for example, a method for generating an electric or pressure difference force that induces a plurality of particles to flow through a through-hole. Independently adjustable heat source in a vicinity of the through-hole induces a thermodynamic force for modifying the flow of the plurality of particles through the through-hole. Additional embodiments are disclosed.
摘要翻译：结合本公开的系统可以包括例如用于产生诱发多个粒子流过通孔的电力或压力差力的方法。在通孔附近的独立可调的热源引起用于改变多个颗粒通过通孔的流动的热力。公开了另外的实施例。

5. 发明授权

US11944966B2 Systems and methods for high-fidelity capture, threading, and infinite-depth sequencing of single nucleic acid molecules 有权
公开(公告)号：US11944966B2
公开(公告)日：2024-04-02
申请号：US17513516
申请日：2021-10-28
申请人： THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS
发明人： Aleksei Aksimentiev
IPC分类号： B01L3/00 , C12Q1/6869
CPC分类号： B01L3/502715 , C12Q1/6869 , B01L2200/0652 , B01L2300/0663 , B01L2300/0896 , B01L2400/0415
摘要： Aspects of the subject disclosure may include, for example, an apparatus comprising: a membrane, wherein the membrane has a first side and a second side, wherein the membrane has a first pore disposed therein, wherein the first pore extends through the membrane from the first side of the membrane to the second side of the membrane, wherein the membrane has a second pore disposed therein, and wherein the second pore extends through the membrane from the first side of the membrane to the second side of the membrane; a first channel disposed on the first side of the membrane, wherein the first channel is along a first longitudinal axis; a second channel disposed on the first side of the membrane, wherein the second channel is along a second longitudinal axis, and wherein the first channel and the second channel are disposed side by side adjacent to each other; a third channel disposed on the second side of the membrane, wherein the third channel is along a third longitudinal axis, wherein the third channel is in first fluid communication with the first channel via the first pore, and wherein the third channel is in second fluid communication with the second channel via the second pore; and one or more sensors disposed at one or more locations to facilitate sequencing of a molecule that extends from the first channel, through the first pore across at least a portion of the third channel, and through the second pore into the second channel. Additional embodiments are disclosed.

6. 发明授权

US11333655B2 Nanopore-based system for trapping, controlled displacement, and sequencing of (bio)macromolecules 有权
公开(公告)号：US11333655B2
公开(公告)日：2022-05-17
申请号：US15697202
申请日：2017-09-06
申请人： THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS , Technische Universiteit Delft
发明人： Aleksei Aksimentiev , Cornelis Dekker
IPC分类号： G01N33/487 , G01N27/447
摘要： Aspects of the subject disclosure may include, for example, an apparatus comprising: a membrane having a first side and a second side, wherein the membrane has first and second pores disposed therein, a processing system; and a memory that stores executable instructions that, when executed by the processing system, facilitate performance of operations comprising: setting a first physical characteristic in a vicinity of the first pore to cause a first end of a molecule having the first end and a second end to be moved through the first pore; setting a second physical characteristic in a vicinity of the second pore to cause the second end of the molecule to be moved through the second pore; and adjusting the first physical characteristic to cause the molecule to be tightened, with a first given amount of force, between the first pore and the second pore. Additional embodiments are disclosed.

7. 发明申请

US20220134337A1 SYSTEMS AND METHODS FOR HIGH-FIDELITY CAPTURE, THREADING, AND INFINITE-DEPTH SEQUENCING OF SINGLE NUCLEIC ACID MOLECULES 有权
公开(公告)号：US20220134337A1
公开(公告)日：2022-05-05
申请号：US17513516
申请日：2021-10-28
申请人： THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS
发明人： Aleksei Aksimentiev
IPC分类号： B01L3/00 , C12Q1/6869
摘要： Aspects of the subject disclosure may include, for example, an apparatus comprising: a membrane, wherein the membrane has a first side and a second side, wherein the membrane has a first pore disposed therein, wherein the first pore extends through the membrane from the first side of the membrane to the second side of the membrane, wherein the membrane has a second pore disposed therein, and wherein the second pore extends through the membrane from the first side of the membrane to the second side of the membrane; a first channel disposed on the first side of the membrane, wherein the first channel is along a first longitudinal axis; a second channel disposed on the first side of the membrane, wherein the second channel is along a second longitudinal axis, and wherein the first channel and the second channel are disposed side by side adjacent to each other; a third channel disposed on the second side of the membrane, wherein the third channel is along a third longitudinal axis, wherein the third channel is in first fluid communication with the first channel via the first pore, and wherein the third channel is in second fluid communication with the second channel via the second pore; and one or more sensors disposed at one or more locations to facilitate sequencing of a molecule that extends from the first channel, through the first pore across at least a portion of the third channel, and through the second pore into the second channel. Additional embodiments are disclosed.

8. 发明申请

US20180088104A1 Nanopore-Based System for Trapping, Controlled Displacement, and Sequencing of (bio)Macromolecules 审中-公开
公开(公告)号：US20180088104A1
公开(公告)日：2018-03-29
申请号：US15697202
申请日：2017-09-06
申请人： The Board of Trustees of the University of Illinois
发明人： Aleksei Aksimentiev , Cornelis Dekker
IPC分类号： G01N33/487 , G01N27/447
CPC分类号： G01N33/48721 , G01N27/44791
摘要： Aspects of the subject disclosure may include, for example, an apparatus comprising: a membrane having a first side and a second side, wherein the membrane has first and second pores disposed therein, a processing system; and a memory that stores executable instructions that, when executed by the processing system, facilitate performance of operations comprising: setting a first physical characteristic in a vicinity of the first pore to cause a first end of a molecule having the first end and a second end to be moved through the first pore; setting a second physical characteristic in a vicinity of the second pore to cause the second end of the molecule to be moved through the second pore; and adjusting the first physical characteristic to cause the molecule to be tightened, with a first given amount of force, between the first pore and the second pore. Additional embodiments are disclosed.

9. 发明申请

US20140360876A1 METHOD AND APPARATUS FOR CONTROLLING MATERIALS THROUGH A THROUGH-HOLE 有权
标题翻译：通过一个孔通过控制材料的方法和装置
公开(公告)号：US20140360876A1
公开(公告)日：2014-12-11
申请号：US13911333
申请日：2013-06-06
申请人： THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS
发明人： Aleksei Aksimentiev
IPC分类号： G01N27/447
CPC分类号： G01N33/48721 , C12Q1/6869 , C12Q2565/531 , C12Q2565/631
摘要： A system that incorporates the subject disclosure may include, for example, a method for selectively applying an electrical potential to a top surface of a membrane having a nanopore to repel or attract a molecular strand from the top surface of the membrane, applying a second electrical potential to a bottom surface of the membrane to repel or attract the molecular strand from the bottom surface of the membrane, applying a third electrical potential to an electrolyte solution to apply a transport force on the molecular strand to displace a section of the molecular strand into the nanopore, arresting the section of the molecular strand in the nanopore by adjusting of the first electrical potential, the second electrical potential, the third electrical potential, or combinations thereof, and measuring a signal at the nanopore to identify the section of the molecular strand. Other embodiments are disclosed.
摘要翻译：结合本公开的系统可以包括例如用于选择性地将电位施加到具有纳米孔的膜的顶表面以从膜的顶表面排斥或吸引分子束的方法，施加第二电从膜的底部表面排斥或吸引分子链，将第三电位施加到电解质溶液上以在分子链上施加输送力以将分子链的一部分置换成纳米孔，通过调节第一电位，第二电位，第三电位或其组合来阻止纳米孔中的分子链的截面，并测量纳米孔处的信号以鉴定分子链的部分。公开了其他实施例。

10. 发明公开

US20230420045A1 DNA-Based Data Storage Systems 审中-公开
公开(公告)号：US20230420045A1
公开(公告)日：2023-12-28
申请号：US18171764
申请日：2023-02-21
申请人： The Board of Trustees of the University of Illinois
发明人： Olgica Milenkovic , Charles M. Schroeder , Seyedkasra Tabatabaei , Aleksei Aksimentiev , Alvaro Gonzalo Hernandez , Chao Pan , Jingqian Liu , Shubham Chandak , Bach Pham , Min Chen , Spencer A. Shorkey
IPC分类号： G11C13/02 , G16B50/30 , G16B40/00 , G16B30/00 , C12Q1/6869
CPC分类号： G11C13/02 , G16B50/30 , G16B40/00 , G16B30/00 , C12Q1/6869
摘要： The present disclosure relates generally to data storage using DNA sequences comprising synthetic nucleotides. In particular, the disclosure provides for a DNA data storage system comprising a covalently linked sequence of nucleotides, wherein the sequence of nucleotides comprises a modification region, wherein the nucleotides comprise synthetic nucleotides.

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