专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US5320958A Isolated bacterial reverse transcriptase 失效
标题翻译：分离的细菌逆转录酶
公开(公告)号：US5320958A
公开(公告)日：1994-06-14
申请号：US315316
申请日：1989-02-24
申请人： Sumiko Inouye , Mei-Yin Hsu , Susan Eagle , Masayori Inouye
发明人： Sumiko Inouye , Mei-Yin Hsu , Susan Eagle , Masayori Inouye
IPC分类号： C07H21/04 , C07K14/00 , C07K14/195 , C07K14/41 , C12N9/12 , C12N15/09 , C12N15/54 , C12R1/01 , C12N1/00 , C12N1/12
CPC分类号： C12N9/1276 , Y10S435/822
摘要： The present invention relates to an isolated bacterial reverse transcriptase. The reverse transcriptase synthesizes a peculiar RNA-DNA complex called msDNA which is a single-stranded DNA structure branched out from an RNA molecule. The gene coding for the reverse transcriptase has been isolated and sequenced. It codes for a polypeptide of 485 amino acid residues. This is the first time that a reverse transcriptase has been found, identified and isolated from a prokaryotic microorganism of which the amino acid sequence is shown in FIGS. 2a-2d.
摘要翻译：本发明涉及分离的细菌逆转录酶。逆转录酶合成称为msDNA的特异性RNA-DNA复合物，其是从RNA分子分支的单链DNA结构。编码逆转录酶的基因已被分离并测序。它编码485个氨基酸残基的多肽。这是第一次从原核生物中发现，鉴定和分离了逆转录酶，其中氨基酸序列显示在图1和2中。 2a-2d。

2. 发明授权

US5780269A Hybrid molecules 失效
标题翻译：杂交分子
公开(公告)号：US5780269A
公开(公告)日：1998-07-14
申请号：US503730
申请日：1995-07-18
申请人： Sumiko Inouye , Masayori Inouye
发明人： Sumiko Inouye , Masayori Inouye
IPC分类号： C12N9/12 , C12N15/10 , C12P19/34 , C12N15/74
CPC分类号： C12N9/1276 , C12N15/10 , C12P19/34
摘要： Novel hybrid molecules comprising an RNA and a DNA portion are disclosed. A method for synthesizing the molecule is disclosed.
摘要翻译：公开了包含RNA和DNA部分的新型杂合分子。公开了一种合成该分子的方法。

3. 发明授权

US6043028A Method for synthesizing single-stranded stem-loop DNA's the products and uses therefore 失效
标题翻译：单链茎环DNA的合成方法及其用途
公开(公告)号：US6043028A
公开(公告)日：2000-03-28
申请号：US430277
申请日：1995-04-28
申请人： Atsushi Ohshima , Sumiko Inouye , Masayori Inouye
发明人： Atsushi Ohshima , Sumiko Inouye , Masayori Inouye
IPC分类号： C12N15/09 , C07H21/04 , C12N1/19 , C12N1/21 , C12N9/38 , C12N9/86 , C12N15/10 , C12N15/113 , C12N15/70 , C12N15/81 , C12Q1/68 , C12R1/19 , C12R1/865 , C12N15/64 , C12P19/34
CPC分类号： C12N15/113 , C12N15/10 , C12N15/70 , C12N15/81 , C12N9/2471 , C12N9/86 , C12Q1/6853 , C12Q1/6867 , C12Y302/01023 , C12N2310/111 , C12N2310/15 , C12N2310/53
摘要： A method of synthesis of new and useful single-stranded DNAs which have a stem-loop configuration (ss-slDNA). The method is an in vivo or an in vitro synthesis. Replicating vehicles which produce these ss-slDNAs. The ss-slDNAs are described. Uses for these slDNAs are disclosed. They can be used for introducing random mutations, they lend themselves for replication by a variant of the PCR method. They can also be used for regulating gene function. Other uses are disclosed.
摘要翻译：合成具有茎 - 环构型（ss-slDNA）的新的和有用的单链DNA的方法。该方法是体内或体外合成。复制产生这些ss-SLDNA的载体。描述了ss-SLDNA。公开了这些用于这些slDNA的用途。它们可用于引入随机突变，它们通过PCR方法的变体使其复制。它们也可用于调节基因功能。披露了其他用途。

4. 发明授权

US6017737A E. coli msDNA synthesizing system, products and uses 失效
标题翻译：大肠杆菌msDNA合成系统，产品及用途
公开(公告)号：US6017737A
公开(公告)日：2000-01-25
申请号：US419810
申请日：1995-04-11
申请人： Masayori Inouye , Sumiko Inouye
发明人： Masayori Inouye , Sumiko Inouye
IPC分类号： C12N15/10 , C12N15/52 , C12N15/66 , C12P19/34
CPC分类号： C12N15/10
摘要： A multicopy single-stranded DNA (msDNA) synthesizing system in E. coli is disclosed. The use of the msDNA system to synthesize cDNA in vivo is disclosed. Construction of synthetic msDNA is also disclosed. Also processes for gene amplification and for producing a stable RNA are disclosed.
摘要翻译：公开了在大肠杆菌中的多拷贝单链DNA（msDNA）合成系统。公开了使用msDNA系统在体内合成cDNA。还公开了合成msDNA的构建。还公开了用于基因扩增和产生稳定的RNA的过程。

5. 发明授权

US5079151A Production of branched RNA-linked multi-copy single-stranded DNA using permeabilized cells 失效
标题翻译：使用透化细胞生产支链RNA连接的多拷贝单链DNA
公开(公告)号：US5079151A
公开(公告)日：1992-01-07
申请号：US315427
申请日：1989-02-24
申请人： Bert C. Lampson , Masayori Inouye , Sumiko Inouye
发明人： Bert C. Lampson , Masayori Inouye , Sumiko Inouye
IPC分类号： C07H21/04 , C12N15/09 , C12N15/10 , C12P19/34 , C12R1/01
CPC分类号： C12N15/10 , C12P19/34 , Y10S435/822
摘要： msDNA is a peculiar molecule consisting of a branched RNA linked to single-stranded DNA via a 2', 5' phosphodiester bond. A cell-free system, utilizing cells permeablized with phenethyl, alcohol, was established to study the synthesis of msDNA in Myxococcus xanthus. Permeablized cell labeled with [.alpha.-.sup.32 P]dCTP in the presence of ddGTP, ddATP, or ddTTP, produce a band that migrates at the same position as the full-sized msDNA in an acrylamide gel. However, when this band is treated with ribonuclease A prior to gel electrophoresis, it resulted in many bands of different sizes. This indicates that during the labeling, intermediates are produced in which single-stranded DNAs of various lengths are associated with a compensatory length of RNA such that the total length for each intermediate is identical. These results provide evidence for the previously proposed model in which msDNA is synthesized by reverse transcriptase using a folded RNA precursor as a primer as well as a template. Furthermore, it was found that there is a precise coupling mechanism of reverse transcriptase and ribonuclease H.
摘要翻译： msDNA是由通过2'，5'磷酸二酯键与单链DNA连接的支链RNA组成的特殊分子。建立了一种无细胞系统，利用渗透有苯乙醇，醇的细胞，研究了黄连球菌中msDNA的合成。在ddGTP，ddATP或ddTTP存在下用[α-32P] dCTP标记的渗透细胞产生在与丙烯酰胺凝胶中的全尺寸msDNA相同位置迁移的条带。然而，当在胶束电泳之前用核糖核酸酶A处理该条带时，其产生许多不同大小的条带。这表明在标记过程中产生了中间体，其中各种长度的单链DNA与代偿性长度的RNA相关联，使得每个中间体的总长度相同。这些结果为先前提出的模型提供了证据，其中使用折叠的RNA前体作为引物以及模板通过逆转录酶合成了msDNA。此外，发现有逆转录酶和核糖核酸酶H的精确偶联机制。

6. 发明授权

US5714323A Over expression of single-stranded molecules 失效
标题翻译：单链分子的过表达
公开(公告)号：US5714323A
公开(公告)日：1998-02-03
申请号：US318867
申请日：1995-05-04
申请人： Atushi Ohshima , Sumiko Inouye , Masayori Inouye
发明人： Atushi Ohshima , Sumiko Inouye , Masayori Inouye
IPC分类号： C12N15/10 , C12N15/70 , C12Q1/68 , C12P19/34
CPC分类号： C12N15/70 , C12N15/10 , C12N2310/111
摘要： A method of synthesis of new and useful single-stranded DNAs which have a stem-loop configuration (ss-slDNA). The method is an in vivo or an in vitro synthesis. Replicating vehicles which produce these ss-slDNAs. The ss-slDNAs are described. Uses for these slDNAs are disclosed. They can be used for introducing random mutations, they lend themselves for replication by a variant of the PCR method. They can also be used for regulating gene function. Other uses are disclosed.
摘要翻译： PCT No.PCT / US94 / 02169 Sec。 371日期：1995年5月4日 102（e）日期1995年5月4日PCT 1994年3月1日PCT公布。出版物WO94 / 20639 日期1994年9月15日一种合成具有茎 - 环构型（ss-slDNA）的新型和有用的单链DNA的方法。该方法是体内或体外合成。复制产生这些ss-SLDNA的载体。描述了ss-SLDNA。公开了这些用于这些slDNA的用途。它们可用于引入随机突变，它们通过PCR方法的变体使其复制。它们也可用于调节基因功能。披露了其他用途。

7. 发明授权

US5405775A Retrons coding for hybrid DNA/RNA molecules 失效
标题翻译：编码杂交DNA / RNA分子的核苷酸
公开(公告)号：US5405775A
公开(公告)日：1995-04-11
申请号：US518749
申请日：1990-05-02
申请人： Masayori Inouye , Sumiko Inouye
发明人： Masayori Inouye , Sumiko Inouye
IPC分类号： C12N9/12 , C12N15/10 , C12P19/34 , C12N1/21 , C12N15/52 , C12N15/70
CPC分类号： C12N9/1276 , C12N15/10 , C12P19/34
摘要： A multicopy single-stranded DNA (msDNA) synthesizing system in E. coli is disclosed. The use of the msDNA system to synthesize cDNA in vivo is disclosed. Construction of synthetic msDNA is also disclosed. Also processes for gene amplification and for producing a stable RNA are disclosed.
摘要翻译：公开了在大肠杆菌中的多拷贝单链DNA（msDNA）合成系统。公开了使用msDNA系统在体内合成cDNA。还公开了合成msDNA的构建。还公开了用于基因扩增和产生稳定的RNA的过程。

8. 发明授权

US5849563A Eukaryotes expressing single stranded hybrid molecules 失效
标题翻译：表达单链杂交分子的真核生物
公开(公告)号：US5849563A
公开(公告)日：1998-12-15
申请号：US507634
申请日：1995-07-25
申请人： Shohei Miyata , Atsushi Ohshima , Sumiko Inouye , Masayori Inouye
发明人： Shohei Miyata , Atsushi Ohshima , Sumiko Inouye , Masayori Inouye
IPC分类号： C12N9/12 , C12N15/10 , C12N15/113 , C12N15/81 , C12P19/34 , C12N5/10 , C12N1/15
CPC分类号： C12N15/113 , C12N15/10 , C12N15/81 , C12N9/1276 , C12P19/34 , C12N2310/111
摘要： Eucaryotic cells like mammal and plant cells transfected with genes, called retrons, which code for stable single-stranded hybrid molecules (msDNA) containing RNA and DNA portions are disclosed. The retrons producing said linked RNA and DNA portions of the msDNAs also contain a gene encoding a reverse transcriptase (RT), which is necessary for the synthesis of the msDNAs. The msDNAs may contain foreign nucleic acid fragments which may be antisense fragments. These msDNA antisense vectors are useful for targeting genes of target proteins.
摘要翻译：公开了编码含有RNA和DNA部分的稳定单链杂交分子（msDNA）的真核细胞，如哺乳动物和转染基因的植物细胞，称为retrons。产生所述连接的RNA的核糖体和所述msDNA的DNA部分还含有编码逆转录酶（RT）的基因，其是合成所述msDNA所必需的。 msDNA可以含有可以是反义片段的外来核酸片段。这些msDNA反义载体可用于靶向靶蛋白的基因。

9. 发明授权

US5643762A Method for synthesizing single-stranded stem-loop DNAs, the products and uses therefor 失效
标题翻译：单链茎环DNA的合成方法，产品及用途
公开(公告)号：US5643762A
公开(公告)日：1997-07-01
申请号：US284860
申请日：1994-08-02
申请人： Atsushi Ohshima , Sumiko Inouye , Masayori Inouye
发明人： Atsushi Ohshima , Sumiko Inouye , Masayori Inouye
IPC分类号： C12N15/09 , C12N9/38 , C12N15/10 , C12N15/70 , C12Q1/68 , C12P19/34 , C12N15/11
CPC分类号： C12N15/70 , C12N15/10 , C12N9/2471 , C12Y302/01023 , C12N2310/111 , C12N2310/15 , C12N2310/53
摘要： The invention provides a method for synthesizing new and useful single-stranded DNAs which have a stem-loop configuration (ss-slDNA). The method is an in vivo or in vitro synthesis. Replicating vehicles are provided which produce these ss-slDNAs. The slDNAs can be used for introducing random mutations in a selected gene, and they lend themselves for replication by a variant of the PCR method.
摘要翻译：本发明提供了一种合成具有茎环构型（ss-slDNA）的新型有用的单链DNA的方法。该方法是体内或体外合成。提供产生这些ss-SLDNA的复制载体。可以将slDNA用于在所选择的基因中引入随机突变，并且通过PCR方法的变体将其自身复制。

10. 发明授权

US5436141A Method for synthesizing stable single-stranded CDNA in eukaryotes by means of a bacterial retron and products 失效
标题翻译：通过细菌培养基和产物在真核生物中合成稳定的单链CDNA的方法
公开(公告)号：US5436141A
公开(公告)日：1995-07-25
申请号：US753110
申请日：1991-08-30
申请人： Shohei Miyata , Atsushi Ohshima , Sumiko Inouye , Masayori Inouye
发明人： Shohei Miyata , Atsushi Ohshima , Sumiko Inouye , Masayori Inouye
IPC分类号： C12N1/19 , C12N5/10 , C12N9/12 , C12N15/09 , C12N15/10 , C12N15/113 , C12N15/54 , C12N15/81 , C12P19/34 , C12R1/85
CPC分类号： C12N15/113 , C12N15/10 , C12N15/81 , C12N9/1276 , C12P19/34 , C12N2310/111
摘要： A method for producing in vivo stable single-stranded DNAs in eucaryotic cells. The DNAs are multicopy single-stranded DNA (msDNA) structures constituted by a RNA and a DNA portion. The group of genes (retrons) producing said coupled RNA and DNA portions of the msDNAs and the gene encoding reverse transcriptase (RT). The transformed eucaryotes harboring these retrons. The new msDNAs which are encoded by the new retrons. The msDNAs can be used as vectors for antisense DNA and for amplification of inserted genes.
摘要翻译：用于在真核细胞中产生体内稳定的单链DNA的方法。 DNA是由RNA和DNA部分构成的多拷贝单链DNA（msDNA）结构。产生所述偶联RNA的基因组（retron）和msDNA的DNA部分以及编码逆转录酶（RT）的基因。转化的真核生物藏着这些核子。由新的核糖核酸编码的新的msDNA。可以将这些msDNA用作反义DNA的载体和扩增插入的基因。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式