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1. 发明申请

US20060063734A1 4,7-dichlorofluorescein dyes as molecular probes 审中-公开
标题翻译： 4,7-二氯荧光素染料作为分子探针
公开(公告)号：US20060063734A1
公开(公告)日：2006-03-23
申请号：US11269522
申请日：2005-11-07
申请人： Steven Menchen , Linda Lee , Charles Connell , N. Hershey , Vergine Chakerian , Sam Woo , Steven Fung
发明人： Steven Menchen , Linda Lee , Charles Connell , N. Hershey , Vergine Chakerian , Sam Woo , Steven Fung
IPC分类号： C12Q1/68 , C07H21/04 , A61K48/00
CPC分类号： C07H21/00 , A61K48/00 , C07D311/82 , C09B11/08 , C12Q1/6816 , C12Q1/6869 , Y10S435/968 , Y10S436/80 , Y10T436/143333 , Y10T436/145555 , Y10T436/147777 , C12Q2563/107
摘要： Long wavelength, narrow emission bandwidth fluorecein dyes are provided for detecting spacially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 2′,4′,5′,7′-tetrachloro-4,7-dichloro-5- (and 6-)carboxyfluoresceins. Methods and kits for using the dyes in DNA analysis are provided.
摘要翻译：提供长波长，窄发射带宽荧光素染料用于检测空间重叠的目标物质。染料包括4,7-二氯荧光素，特别是2'，4'，5'，7'-四氯-4,7-二氯-5-（和6-）羧基荧光素。提供了在DNA分析中使用染料的方法和试剂盒。

2. 发明申请

US20050084870A1 4,7-dichlorofluorescein dyes as molecular probes 审中-公开
标题翻译： 4,7-二氯荧光素染料作为分子探针
公开(公告)号：US20050084870A1
公开(公告)日：2005-04-21
申请号：US10714407
申请日：2003-11-14
申请人： Steven Menchen , Linda Lee , Charles Connell , N. Hershey , Vergine Chakerian , Sam Woo , Steven Fung
发明人： Steven Menchen , Linda Lee , Charles Connell , N. Hershey , Vergine Chakerian , Sam Woo , Steven Fung
IPC分类号： C07D311/82 , C07H21/00 , C09B11/08 , C12Q1/68 , C07H21/04
CPC分类号： C07H21/00 , A61K48/00 , C07D311/82 , C09B11/08 , C12Q1/6816 , C12Q1/6869 , Y10S435/968 , Y10S436/80 , Y10T436/143333 , Y10T436/145555 , Y10T436/147777 , C12Q2563/107
摘要： Long wavelength, narrow emission bandwidth fluorescein dyes are provided for detecting specially overlapping target substances. The dyes comprise 4,7-dichlorofluorescein, and particularly 2′, 4′,5′,7′-tetrachloro-4,7-dichloro-5-(and 5-) carboxyfluoresceins. Methods and kits for using the dyes in DNA analysis are provided.
摘要翻译：提供长波长，窄发射带宽荧光素染料，用于检测特别重叠的目标物质。染料包括4,7-二氯荧光素，特别是2'，4'，5'，7'-四氯-4,7-二氯-5-（和5-）羧基荧光素。提供了在DNA分析中使用染料的方法和试剂盒。

3. 发明授权

US5188934A 4,7-dichlorofluorescein dyes as molecular probes 失效
标题翻译： 4,7-DICHLOROFUORESCEIN DYES AS MOLECULAR PROBES
公开(公告)号：US5188934A
公开(公告)日：1993-02-23
申请号：US436455
申请日：1989-11-14
申请人： Steven M. Menchen , Linda G. Lee , Charles R. Connell , N. Davis Hershey , Vergine Chakerian , Sam Woo , Steven Fung
发明人： Steven M. Menchen , Linda G. Lee , Charles R. Connell , N. Davis Hershey , Vergine Chakerian , Sam Woo , Steven Fung
IPC分类号： C07D311/82 , C07H21/00 , C09B11/08 , C12Q1/68 , G01N21/64 , G01N21/78 , G01N27/447 , G01N33/58
CPC分类号： C12Q1/6869 , C07D311/82 , C07H21/00 , C09B11/08 , C12Q1/6816 , Y10S435/968 , Y10S436/80
摘要： Long wavelength, narrow emission bandwidth fluorecein dyes are provided for detecting spacially overlapping target substances. The dyes comprise 4,7-dichlorofluoresceins, and particularly 1',2',7',8'-dibenzo-4,7-dichlorofluoresceins. Methods of using the dyes in automated DNA sequencing are described.
摘要翻译：提供长波长，窄发射带宽荧光素染料用于检测空间重叠的目标物质。染料包括4,7-二氯荧光素，特别是1'，2'，7'，8'-二苯并-4,7-二氯荧光素。描述了在自动DNA测序中使用染料的方法。

4. 发明申请

US20050112609A1 Probe composition and method 失效
标题翻译：探头组成和方法
公开(公告)号：US20050112609A1
公开(公告)日：2005-05-26
申请号：US10825624
申请日：2004-04-14
申请人： Paul Grossman , Steven Fung , Steven Menchen , Sam Woo , Emily Winn-Deen
发明人： Paul Grossman , Steven Fung , Steven Menchen , Sam Woo , Emily Winn-Deen
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q1/6825 , C12Q1/6827 , C12Q1/686 , C12Q1/6862 , C12Q2600/156 , G01N27/447 , Y10T436/143333 , C12Q2525/197 , C12Q2525/203 , C12Q2537/143 , C12Q2565/131 , C12Q2565/125 , C12Q2565/107 , C12Q2521/501 , C12Q2563/155 , C12Q2563/119 , C12Q2549/119
摘要： Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In the method, a mixture of sequence-specific probes are reacted with the target polynucleotide under hybridization conditions, and the hybridized probes are treated to selectively modify those probes which are bound to the target polynucleotide in a base-specific manner. The resulting labeled probes include a polymer chain which imparts to each different-sequence probe, a distinctive ratio of charge/translational frictional drag, and a detectable label. The labeled probes are fractionated by electrophoresis in a non-sieving matrix, and the presence of one or more selected sequences in the target polynucleotide are detected according to the observed electrophoretic migration rates of the labeled probes in a non-sieving medium.
摘要翻译：用于检测靶多核苷酸中的一个或多个选择的多核苷酸区域的方法和组合物。在该方法中，将序列特异性探针的混合物与靶多核苷酸在杂交条件下反应，并对杂交的探针进行处理以选择性地修饰以碱基特异性方式结合靶多核苷酸的那些探针。所得到的标记探针包括赋予每个不同序列探针的聚合物链，电荷/平移摩擦阻力的独特比例和可检测标记。标记的探针通过在非筛选基质中的电泳分级分离，并且根据在非筛选培养基中标记的探针的观察到的电泳迁移速率来检测靶多核苷酸中一个或多个选定序列的存在。

5. 发明申请

US20050112608A1 Probe composition and method 失效
标题翻译：探头组成和方法
公开(公告)号：US20050112608A1
公开(公告)日：2005-05-26
申请号：US10825074
申请日：2004-04-14
申请人： Paul Grossman , Steven Fung , Steven Menchen , Sam Woo , Emily Winn-Deen
发明人： Paul Grossman , Steven Fung , Steven Menchen , Sam Woo , Emily Winn-Deen
IPC分类号： C12N15/09 , B01D57/02 , C12Q1/68 , G01N27/447 , G01N30/88 , G01N33/566
CPC分类号： C12Q1/6869 , C12Q1/6816 , C12Q1/6818 , C12Q1/6827 , C12Q1/6858 , C12Q1/686 , C12Q1/6862 , Y10T436/143333 , C12Q2565/125 , C12Q2525/203 , C12Q2525/197 , C12Q2563/167 , C12Q2535/101 , C12Q2565/629 , C12Q2563/155 , C12Q2563/107 , C12Q2531/137 , C12Q2563/119 , C12Q2549/119 , C12Q2525/161 , C12Q2521/501 , C12Q2563/131 , C12Q2525/101 , C12Q2531/113
摘要： Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In one embodiment of the invention, a plurality of different-sequence probe pairs are added to a target polynucleotide, where each probe pair includes two polynucleotide probe elements which are complementary in sequence to adjacent portions of a selected one of the target sequences in the target polynucleotide. In each probe pair, one of the probe elements contains a non-polynucleotide polymer chain which imparts a distinctive mobility to the associated probe pair, when the elements in the pair are ligated. The other element in the pair contains a detectable reporter label. After the probe pairs have been allowed to hybridize with the target polynucleotide, the hybridized polynucleotides are treated under conditions effective to ligate the end subunits of target-bound probe elements when their end subunits are base-paired with adjacent target bases. The ligated probe pairs are then released from the target polynucleotide and separated electrophoretically in a sieving matrix, or chromatographically.
摘要翻译：用于检测靶多核苷酸中的一个或多个选择的多核苷酸区域的方法和组合物。在本发明的一个实施方案中，将多个不同序列探针对加入到靶多核苷酸中，其中每个探针对包含两个多核苷酸探针元件，该多核苷酸探针元件与目标中所选择的一个目标序列的相邻部分互补多核苷酸。在每个探针对中，探针元件中的一个包含非多核苷酸聚合物链，当对中的元件被连接时，赋予相关探针对的特异性移动性。该对中的另一个元素包含可检测的报告物标记。在探针对已被允许与靶多核苷酸杂交后，杂交的多核苷酸在有效连接靶结合探针元件的末端亚基的条件下处理，当它们的末端亚基与相邻的靶基碱基配对时。然后将连接的探针对从目标多核苷酸中释放，并在筛分基质中电泳分离，或进行色谱分离。

6. 发明授权

US08703497B2 Nanowire-based system for analysis of nucleic acids 有权
标题翻译：基于纳米线的核酸分析系统
公开(公告)号：US08703497B2
公开(公告)日：2014-04-22
申请号：US13487638
申请日：2012-06-04
申请人： Hongye Sun , Steven Fung , Sam Woo
发明人： Hongye Sun , Steven Fung , Sam Woo
IPC分类号： G01N27/00 , G01N27/403 , C12M1/34 , B82Y15/00 , G01N27/04 , G01N27/22
CPC分类号： C12Q1/6874 , C12Q1/6811 , C12Q1/6869 , G01N27/3276 , G01N27/3278 , Y10T436/14 , Y10T436/142222 , Y10T436/143333 , C12Q2565/607 , C12Q2533/101 , C12Q2565/133 , C12Q2563/155
摘要： A system and method for analyzing nucleic acids is presented herein. The system can include a nanowire assembly including a plurality of nanowires. In some embodiments, at least two nanowires can be coupled to a different probe corresponding to a different sequence version of a polymorphism. The system can include a reagent delivery system capable of delivering a template polymorphic nucleotide, an extension nucleic acid, and an enzyme to the nanowire assembly. The system can include an electrical detector configured to measure an electrical characteristic of the nanowire assembly and/or a controller in communication with the reagent delivery system. In some embodiments, the electrical detector can be configured to collect data corresponding to changes in the electrical characteristic in response to contact with the template polymorphic nucleotide, the extension nucleic acid, and the enzyme.
摘要翻译：本文提供了用于分析核酸的系统和方法。该系统可以包括包括多个纳米线的纳米线组件。在一些实施例中，至少两个纳米线可以耦合到对应于多态性的不同序列形式的不同探针。该系统可以包括能够将模板多核苷酸，延伸核酸和酶递送至纳米线组件的试剂递送系统。该系统可以包括被配置为测量纳米线组件和/或与试剂递送系统连通的控制器的电特性的电检测器。在一些实施方案中，电检测器可被配置为响应于与模板多核苷酸，延伸核酸和酶的接触而收集对应于电特性变化的数据。

7. 发明申请

US20070238186A1 Nanowire-based system for analysis of nucleic acids 审中-公开
标题翻译：基于纳米线的核酸分析系统
公开(公告)号：US20070238186A1
公开(公告)日：2007-10-11
申请号：US11394147
申请日：2006-03-29
申请人： Hongye Sun , Steven Fung , Sam Woo
发明人： Hongye Sun , Steven Fung , Sam Woo
IPC分类号： G01N33/00 , G08B7/06
CPC分类号： C12Q1/6874 , C12Q1/6811 , C12Q1/6869 , G01N27/3276 , G01N27/3278 , Y10T436/14 , Y10T436/142222 , Y10T436/143333 , C12Q2565/607 , C12Q2533/101 , C12Q2565/133 , C12Q2563/155
摘要： System for detection and/or analysis of nucleic acids using nanowires to detect covalent modification of nucleic acids.
摘要翻译：用于使用纳米线检测和/或分析核酸以检测核酸共价修饰的系统。

8. 发明申请

US20070062583A1 Device and method for making discrete volumes of a first fluid in contact with a second fluid, which are immiscible with each other 有权
标题翻译：使与第二流体接触的第一流体的离散体积彼此不混溶的装置和方法
公开(公告)号：US20070062583A1
公开(公告)日：2007-03-22
申请号：US11508044
申请日：2006-08-22
申请人： David Cox , Willy Wiyatno , Mark Oldham , James Nurse , Douglas Greiner , Sam Woo , Richard Reel , Dennis Lehto , Linda Lee , Janusz Wojtowicz
发明人： David Cox , Willy Wiyatno , Mark Oldham , James Nurse , Douglas Greiner , Sam Woo , Richard Reel , Dennis Lehto , Linda Lee , Janusz Wojtowicz
IPC分类号： G01N1/28 , G01N1/14
CPC分类号： C12Q1/6869 , B01J2219/00353 , B01J2219/0036 , B01J2219/00364 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00626 , B01J2219/00637 , B01J2219/00653 , B01J2219/00657 , B01J2219/00659 , B01L3/0293 , B01L3/502715 , B01L3/502784 , B01L7/52 , B01L2200/0673 , B01L2200/10 , B01L2300/0864 , B01L2300/0867 , B01L2400/0421 , B01L2400/0487 , C12Q1/6806 , C12Q1/6874 , F15C5/00 , F16K99/0001 , F16K99/0011 , F16K99/0013 , F16K2099/0084 , G01N1/14 , G01N27/44743 , G01N27/44769 , G01N35/08 , Y10T137/0318 , Y10T137/4259 , Y10T137/85978 , Y10T137/85986 , Y10T137/86863 , Y10T436/2575 , C12Q2535/101
摘要： Various embodiments described in the application relate to an apparatus, system, and method for generating, within a conduit, discrete volumes of one or more fluids that are immiscible with a second fluid. The discrete volumes can be used for biochemical or molecular biology procedures involving small volumes, for example, microliter-sized volumes, nanoliter-sized volumes, or smaller. The system can comprise an apparatus comprising at least one conduit operatively connected to one or more pumps for providing discrete volumes separated from one another by a fluid that is immiscible with the fluid(s) of the discrete volumes, for example, aqueous immiscible-fluid-discrete volumes separated by an oil.
摘要翻译：在本申请中描述的各种实施例涉及用于在管道内产生与第二流体不混溶的一种或多种流体的离散体积的装置，系统和方法。离散体积可用于涉及小体积的生物化学或分子生物学过程，例如微升体积，纳升级体积或更小体积。该系统可以包括一种装置，该装置包括至少一个导管，其操作地连接到一个或多个泵，用于提供通过与离散体积的流体不混溶的流体彼此分离的离散体积，例如水不混溶流体 - 油分离体积。

9. 发明授权

US09194772B2 Apparatus, system, and method using immiscible-fluid-discrete-volumes 有权
标题翻译：使用不混溶流体离散体的装置，系统和方法
公开(公告)号：US09194772B2
公开(公告)日：2015-11-24
申请号：US12557488
申请日：2009-09-10
申请人： Linda Lee , Sam Woo , Congcong Ma , Richard Reel , Mark Oldham , David Cox , Benjamin Schroeder , Jon Sorenson , Willy Wiyatno
发明人： Linda Lee , Sam Woo , Congcong Ma , Richard Reel , Mark Oldham , David Cox , Benjamin Schroeder , Jon Sorenson , Willy Wiyatno
IPC分类号： C12Q1/68 , C12M1/34 , G01N1/14 , B01L3/00 , F15C5/00 , F16K99/00 , G01N35/08 , B01L3/02 , B01L7/00
CPC分类号： C12Q1/6869 , B01J2219/00353 , B01J2219/0036 , B01J2219/00364 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00626 , B01J2219/00637 , B01J2219/00653 , B01J2219/00657 , B01J2219/00659 , B01L3/0293 , B01L3/502715 , B01L3/502784 , B01L7/52 , B01L2200/0673 , B01L2200/10 , B01L2300/0864 , B01L2300/0867 , B01L2400/0421 , B01L2400/0487 , C12Q1/6806 , C12Q1/6874 , F15C5/00 , F16K99/0001 , F16K99/0011 , F16K99/0013 , F16K2099/0084 , G01N1/14 , G01N27/44743 , G01N27/44769 , G01N35/08 , Y10T137/0318 , Y10T137/4259 , Y10T137/85978 , Y10T137/85986 , Y10T137/86863 , Y10T436/2575 , C12Q2535/101
摘要： Various embodiments of the teachings relate to a system or method for sample preparation or analysis in biochemical or molecular biology procedures. The sample preparation can involve small volume processed in discrete portions or segments or slugs, herein referred to as discrete volumes. A molecular biology procedure can be nucleic acid analysis. Nucleic acid analysis can be an integrated DNA amplification/DNA sequencing procedure.
摘要翻译：本发明的各种实施方案涉及生物化学或分子生物学程序中用于样品制备或分析的系统或方法。样品制备可以涉及在离散部分或段或块中的小体积处理，在本文中称为离散体积。分子生物学程序可以是核酸分析。核酸分析可以是一个综合的DNA扩增/ DNA测序程序。

10. 发明授权

US08703925B2 Chemically-enhanced primer compositions, methods and kits 有权
标题翻译：化学增强底漆组合物，方法和试剂盒
公开(公告)号：US08703925B2
公开(公告)日：2014-04-22
申请号：US13397626
申请日：2012-02-15
申请人： Linda Lee , Sam Woo , Peter Ma
发明人： Linda Lee , Sam Woo , Peter Ma
IPC分类号： C07H21/02 , C07H21/04 , B01L3/00 , C12Q1/68 , C12P19/34
CPC分类号： C12Q1/686 , C12Q1/6869 , C12Q1/6874 , C12Q2525/113 , C12Q2525/125
摘要： A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.
摘要翻译：提供化学增强的引物，其包含带负电荷的部分（NCM），具有a）非核酸酶抗性核苷酸间连接的寡核苷酸序列或b）至少一个核酸酶抗性核苷酸间连接。化学增强引物可用于测序和片段分析。还提供了用于合成化学增强引物的方法以及制备用于测序的DNA的方法，测序DNA的方法和含有化学增强的引物的试剂盒。测序DNA的方法可以包括使扩增反应产物与其中过量扩增引物被核酸酶降解并且化学增强引物基本上未降解的组合物接触。

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