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1. 发明授权

US5908763A DNA encoding GM-CSF and a method of producing GM-CSF protein 失效
标题翻译：编码GM-CSF的DNA和生产GM-CSF蛋白的方法
公开(公告)号：US5908763A
公开(公告)日：1999-06-01
申请号：US287019
申请日：1994-08-08
申请人： Steven C. Clark , Randal J. Kaufman , Gordon G. Wong , Elizabeth A. Wang
发明人： Steven C. Clark , Randal J. Kaufman , Gordon G. Wong , Elizabeth A. Wang
IPC分类号： A61K38/00 , C07K14/535 , C12N15/27 , C12N15/70 , C12N15/81 , C12N15/85 , C07K14/53 , C12N15/63
CPC分类号： C12N15/85 , C07K14/535 , C12N15/70 , C12N15/81 , A61K38/00 , C12N2800/206 , C12N2830/00 , C12N2830/702 , C12N2840/105 , C12N2840/44
摘要： A method for preparing and isolating a transformation vector containing CSF/cDNA is described. The method comprises:preparing RNA from a cell that produces CSF;preparing polyadenylated messenger RNA from said RNA;preparing single stranded cDNA from said messenger RNA;converting the single stranded cDNA to double stranded cDNA;inserting the double stranded cDNA into transformation vectors and transforming bacteria with said vector to form colonies;picking pools of 200 to 500 colonies each and isolating plasmid DNA from each pool;transfecting the plasmid DNA into suitable host cells for expressing CSF protein;culturing the transfected cells and assaying the supernatant for CSF activity; andselecting CSF positive pools and screening the colonies used to make the pool to identify a colony having CSF activity. Also described are a cDNA coding for a protein having CSF activity (i.e. CSF/cDNA), a microorganism or cell line transformed with a recombinant vector containing such CSF/cDNA, and a method for producing CSF protein by expressing said CSF/cDNA by culturing a microorganism or cell line. The invention also provides a method of purifying the CSF proteins and the purified proteins so produced.
摘要翻译：描述了制备和分离含有CSF / cDNA的转化载体的方法。该方法包括：从产生CSF的细胞制备RNA; 从所述RNA制备聚腺苷酸化的信使RNA; 从所述信使RNA制备单链cDNA; 将单链cDNA转化为双链cDNA; 将双链cDNA插入转化载体并用所述载体转化细菌以形成菌落; 分别取200至500个菌落的池，每个池中分离出质粒DNA; 将质粒DNA转染到合适的宿主细胞中以表达CSF蛋白; 培养转染的细胞并测定上清液的CSF活性; 并选择CSF阳性池并筛选用于制备池的菌落以鉴定具有CSF活性的菌落。还描述了编码具有CSF活性的蛋白质（即CSF / cDNA）的cDNA，用含有这种CSF / cDNA的重组载体转化的微生物或细胞系，以及通过培养表达所述CSF / cDNA来产生CSF蛋白的方法微生物或细胞系。本发明还提供了纯化CSF蛋白质和如此制备的纯化蛋白质的方法。

2. 发明授权

US5891429A Recombinant human granulocyte-macrophage-colony stimulating factor (GM-CSF) 失效
标题翻译：重组人粒细胞巨噬细胞集落刺激因子（GM-CSF）
公开(公告)号：US5891429A
公开(公告)日：1999-04-06
申请号：US466308
申请日：1995-06-06
申请人： Steven C. Clark , Randal J. Kaufman , Gordon G. Wong , Elizabeth A. Wang
发明人： Steven C. Clark , Randal J. Kaufman , Gordon G. Wong , Elizabeth A. Wang
IPC分类号： A61K38/00 , C07K14/535 , C12N15/27 , C12N15/70 , C12N15/81 , C12N15/85 , A61K38/19 , C07K1/20
CPC分类号： C12N15/85 , C07K14/535 , C12N15/70 , C12N15/81 , A61K38/00 , C12N2800/206 , C12N2830/00 , C12N2830/702 , C12N2840/105 , C12N2840/44
摘要： A method for preparing and isolating a transformation vector containing CSF/cDNA is described. The method comprises:preparing RNA from a cell that produces CSF;preparing polyadenylated messenger RNA from said RNA;preparing single stranded cDNA from said messenger RNA;converting the single stranded cDNA to double stranded cDNA;inserting the double stranded cDNA into transformation vectors and transforming bacteria with said vector to form colonies;picking pools of 200 to 500 colonies each and isolating plasmid DNA from each pool;transfecting the plasmid DNA into suitable host cells for expressing CSF protein;culturing the transfected cells and assaying the supernatant for CSF activity; andselecting CSF positive pools and screening the colonies used to make the pool to identify a colony having CSF activity. Also described are a cDNA coding for a protein having CSF activity (i.e. CSF/cDNA), a microorganism or cell line transformed with a recombinant vector containing such CSF/cDNA, and a method for producing CSF protein by expressing said CSF/cDNA by culturing a microorganism or cell line. The invention also provides a method of purifying the CSF proteins and the purified proteins so produced.
摘要翻译：描述了制备和分离含有CSF / cDNA的转化载体的方法。该方法包括：从产生CSF的细胞制备RNA; 从所述RNA制备聚腺苷酸化的信使RNA; 从所述信使RNA制备单链cDNA; 将单链cDNA转化为双链cDNA; 将双链cDNA插入转化载体并用所述载体转化细菌以形成菌落; 分别取200至500个菌落的池，每个池中分离出质粒DNA; 将质粒DNA转染到合适的宿主细胞中以表达CSF蛋白; 培养转染的细胞并测定上清液的CSF活性; 并选择CSF阳性池并筛选用于制备池的菌落以鉴定具有CSF活性的菌落。还描述了编码具有CSF活性的蛋白质（即CSF / cDNA）的cDNA，用含有这种CSF / cDNA的重组载体转化的微生物或细胞系，以及通过培养表达所述CSF / cDNA来产生CSF蛋白的方法微生物或细胞系。本发明还提供了纯化CSF蛋白质和如此制备的纯化蛋白质的方法。

3. 发明授权

US4675285A Method for identification and isolation of DNA encoding a desired protein 失效
标题翻译：用于鉴定和分离编码所需蛋白质的DNA的方法
公开(公告)号：US4675285A
公开(公告)日：1987-06-23
申请号：US652316
申请日：1984-09-19
申请人： Steven C. Clark , Randal J. Kaufman , Gordon G. Wong
发明人： Steven C. Clark , Randal J. Kaufman , Gordon G. Wong
IPC分类号： C07K14/53 , C12N15/10 , C12N15/85 , C12Q1/68 , C12N5/00 , C12N15/00 , C12P21/00
CPC分类号： C12N15/1086 , C07K14/53 , C12N15/85
摘要： A method for identifying and isolating clones containing DNA coding for a desired protein is described. DNA prepared from a cell that expresses the desired protein is inserted into an isolation expression vector having means for replication (as a means of producing DNA) and a suitable promoter for expression of said DNA in a predetermined mammalian host cell as well as means for replication in a bacterial cell. The transient expression vector is then inserted into a bacterial cell for replication of the DNA. Pools of DNA, prepared from a predetermined number of bacterial clones so that the nucleic acids (DNA and RNA) is substantially free of other bacterial contaminants are transfected or microinjected into mammalian host cells and conditioned medium from growing such cells is tested for the presence of the desired protein. Positive pools are selected and the clones used to make the pool are screened to identify and isolate the clone containing the desired DNA.
摘要翻译：描述了鉴定和分离含有编码所需蛋白质的DNA的克隆的方法。将从表达所需蛋白质的细胞制备的DNA插入具有复制方式的分离表达载体（作为生产DNA的手段）和用于在预定哺乳动物宿主细胞中表达所述DNA的合适启动子以及复制手段在细菌细胞中。然后将瞬时表达载体插入用于DNA复制的细菌细胞中。从预定数量的细菌克隆制备DNA，使得核酸（DNA和RNA）基本上不含其它细菌污染物的DNA转染或显微注射到哺乳动物宿主细胞中，并测试来自培养这样的细胞的条件培养基的存在所需蛋白质。选择阳性池，筛选用于制备池的克隆以鉴定并分离含有所需DNA的克隆。

4. 发明授权

US5942221A Recombinant primate granulocyte macrophage-colony stimulating factor 失效
标题翻译：重组灵长类动物粒细胞巨噬细胞集落刺激因子
公开(公告)号：US5942221A
公开(公告)日：1999-08-24
申请号：US468694
申请日：1995-06-06
申请人： Steven C. Clark , Randal J. Kaufman , Gordon G. Wong
发明人： Steven C. Clark , Randal J. Kaufman , Gordon G. Wong
IPC分类号： C12P21/02 , A61K38/00 , A61K38/17 , C07K14/535 , C12N15/27 , C12N15/70 , C12N15/81 , C12N15/85 , A61K38/19
CPC分类号： C07K14/535 , C12N15/70 , C12N15/81 , C12N15/85 , A61K38/00 , C12N2830/42 , C12N2840/105 , Y10S514/885
摘要： A method for preparing and isolating a transformation vector containing CSF/cDNA is described. The method comprises: preparing RNA from a cell that produces CSF; preparing polyadenylated messenger RNA from said RNA; preparing single stranded cDNA from said messenger RNA; converting the single stranded cDNA to double stranded cDNA; inserting the double stranded cDNA into transformation vectors and transforming bacteria with said vector to form colonies; picking pools of 200 to 500 colonies each and isolating plasmid DNA from each pool; transfecting the plasmid DNA into suitable host cells for expressing CSF protein; culturing the transfected cells and assaying the supernatant for CSF activity; and selecting CSF positive pools and screening the colonies used to make the pool to identify a colony having CSF activity. Also described are a cDNA coding for a protein having CSF activity (i.e. CSF/cDNA), a microorganism or cell line transformed with a recombinant vector containing such CSF/cDNA, and a method for producing CSF protein by expressing said CSF/cDNA by culturing a microorganism or cell line.
摘要翻译：描述了制备和分离含有CSF / cDNA的转化载体的方法。该方法包括：从产生CSF的细胞制备RNA; 从所述RNA制备聚腺苷酸化的信使RNA; 从所述信使RNA制备单链cDNA; 将单链cDNA转化为双链cDNA; 将双链cDNA插入转化载体并用所述载体转化细菌以形成菌落; 分别取200至500个菌落的池，每个池中分离出质粒DNA; 将质粒DNA转染到合适的宿主细胞中以表达CSF蛋白; 培养转染的细胞并测定上清液的CSF活性; 并选择CSF阳性池并筛选用于制备池的菌落以鉴定具有CSF活性的菌落。还描述了编码具有CSF活性的蛋白质（即CSF / cDNA）的cDNA，用含有这种CSF / cDNA的重组载体转化的微生物或细胞系，以及通过培养表达所述CSF / cDNA来产生CSF蛋白的方法微生物或细胞系。

5. 发明授权

US07122633B2 Natural killer stimulatory factor 失效
标题翻译：自然杀伤刺激因子
公开(公告)号：US07122633B2
公开(公告)日：2006-10-17
申请号：US10358395
申请日：2003-02-05
申请人： Giorgio Trinchieri , Bice Perussia , Stanley F. Wolf , Steven C. Clark , Gordon G. Wong , Rodney Hewick , Michiko Kobayashi
发明人： Giorgio Trinchieri , Bice Perussia , Stanley F. Wolf , Steven C. Clark , Gordon G. Wong , Rodney Hewick , Michiko Kobayashi
IPC分类号： C07K14/64
CPC分类号： C07K14/5434 , A61K38/00 , C07K14/52
摘要： This application relates to antibodies reactive with a novel homogenous human cytokine, Natural Killer Stimulator Factor (NKSF), having the ability to induce the production of gamma interferon in vitro in human peripheral blood lymphocytes, and a pharmaceutical preparation containing such antibodies.
摘要翻译：本申请涉及与人外周血淋巴细胞体外诱导γ干扰素生成能力的新型均质人细胞因子天然杀伤剂刺激因子（NKSF）反应的抗体，以及含有该抗体的药物制剂。

6. 发明授权

US5811523A Antibodies to natural killer stimulatory factor 失效
公开(公告)号：US5811523A
公开(公告)日：1998-09-22
申请号：US956240
申请日：1997-10-22
申请人： Giorgio Trinchieri , Bice Perussia , Steven C. Clark , Gordon G. Wong , Rodney Hewick , Michiko Kobayashi , Stanley F. Wolf
发明人： Giorgio Trinchieri , Bice Perussia , Steven C. Clark , Gordon G. Wong , Rodney Hewick , Michiko Kobayashi , Stanley F. Wolf
IPC分类号： A61K38/00 , C07K14/54 , C07K16/24
CPC分类号： C07K14/5434 , C07K14/52 , A61K38/00
摘要： A novel homogeneous human cytokine, Natural Killer Stimulatory Factor, having the ability to induce the production of gamma interferon in vitro in human peripheral blood lymphocytes, and a pharmaceutical preparation containing it.

7. 发明授权

US06555658B2 Antibodies to natural killer stimulatory factor 失效
标题翻译：抗自然杀伤刺激因子的抗体
公开(公告)号：US06555658B2
公开(公告)日：2003-04-29
申请号：US09924703
申请日：2001-08-09
申请人： Giorgio Trinchieri , Bice Perussia , Steven C. Clark , Gordon G. Wong , Rodney Hewick , Michiko Kobayashi
发明人： Giorgio Trinchieri , Bice Perussia , Steven C. Clark , Gordon G. Wong , Rodney Hewick , Michiko Kobayashi
IPC分类号： C07K1624
CPC分类号： C07K14/5434 , A61K38/00 , C07K14/52
摘要： This application relates to antibodies reactive with a novel homogenous human cytokine, Natural Killer Stimulator Factor (NKSF), having the ability to induce the production of gamma interferon in vitro in human peripheral blood lymphocytes, and a pharmaceutical preparation containing such antibodies.
摘要翻译：本申请涉及与人外周血淋巴细胞体外诱导γ干扰素生成能力的新型均质人细胞因子天然杀伤剂刺激因子（NKSF）反应的抗体，以及含有该抗体的药物制剂。

8. 发明授权

US06300478B1 Antibodies to natural killer stimulatory factor 失效
标题翻译：抗自然杀伤刺激因子的抗体
公开(公告)号：US06300478B1
公开(公告)日：2001-10-09
申请号：US09325958
申请日：1999-06-04
申请人： Giorgio Trinchieri , Bice Perussia , Steven C. Clark , Gordon G. Wong , Rodney Hewick , Michiko Kobayashi
发明人： Giorgio Trinchieri , Bice Perussia , Steven C. Clark , Gordon G. Wong , Rodney Hewick , Michiko Kobayashi
IPC分类号： C07K1624
CPC分类号： C07K14/5434 , A61K38/00 , C07K14/52
摘要： Antibodies reactive with a novel homogeneous human cytokine, Natural Killer Stimulatory Factor, (NKSF), NKSF having the ability to induce the production of gamma interferon in vitro in human peripheral blood lymphocytes, and a pharmaceutical preparation containing such antibodies.
摘要翻译：与人外周血淋巴细胞体外诱导γ干扰素生成能力的新型均质人细胞因子，天然杀伤刺激因子（NKSF），NKSF反应的抗体以及含有该抗体的药物制剂。

9. 发明授权

US06284237B1 Methods of treatment using IL-6 失效
标题翻译：使用IL-6治疗方法
公开(公告)号：US06284237B1
公开(公告)日：2001-09-04
申请号：US08224985
申请日：1994-04-06
申请人： Steven C. Clark , Gordon G. Wong , Paul Schendel , John McCoy
发明人： Steven C. Clark , Gordon G. Wong , Paul Schendel , John McCoy
IPC分类号： A61K3820
CPC分类号： C07K14/5412 , A61K38/2013 , A61K38/204 , A61K38/217 , A61K39/395 , Y10S514/885 , A61K2300/00
摘要： IL-6 is produced via recombinant DNA techniques. The peptide is useful in the treatment of disorders characterized by deficiencies in hematopoietic cells and in combination with other hematopoietins in cancer therapies.
摘要翻译：通过重组DNA技术产生IL-6。该肽可用于治疗以造血细胞缺陷为特征的疾病，并与癌症治疗中的其它造血素组合。

10. 发明授权

US5569454A Methods of treating infection using natural killer stimulatory factor 失效
标题翻译：使用自然杀伤刺激因子治疗感染的方法
公开(公告)号：US5569454A
公开(公告)日：1996-10-29
申请号：US403086
申请日：1995-03-13
申请人： Giorgio Trinchieri , Bice Perussia , Steven C. Clark , Gordon G. Wong , Rodney Hewick , Michiko Kobayashi , Stanley F. Wolf
发明人： Giorgio Trinchieri , Bice Perussia , Steven C. Clark , Gordon G. Wong , Rodney Hewick , Michiko Kobayashi , Stanley F. Wolf
IPC分类号： A61K38/00 , A61K38/22 , A61K39/395 , A61P31/00 , A61P31/04 , A61P31/12 , A61P31/18 , A61P35/00 , A61P35/02 , A61P37/04 , A61P43/00 , C07K1/16 , C07K1/20 , C07K1/22 , C07K14/00 , C07K14/435 , C07K14/52 , C07K14/54 , C07K16/24 , C12N1/21 , C12N5/00 , C12N5/10 , C12N15/00 , C12N15/09 , C12N15/19 , C12N15/26 , C12P21/00 , C12P21/02 , C12R1/19 , C12R1/91 , G01N33/53 , A61K38/20
CPC分类号： C07K14/5434 , A61K38/00 , Y10S435/803 , Y10S514/885
摘要： Methods of treating an infection using Natural Killer Stimulatory Factor (NKSF) are disclosed.
摘要翻译：公开了使用天然杀伤刺激因子（NKSF）治疗感染的方法。

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