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1. 发明申请

US20120219531A1 Microcarriers for Stem Cell Culture 审中-公开
标题翻译：干细胞培养的微载体
公开(公告)号：US20120219531A1
公开(公告)日：2012-08-30
申请号：US13296526
申请日：2011-11-15
申请人： Steve Oh , Shaul Reuveny , Allen Chen , Jerry Kok Yen Chan , Zhiyong Zhang
发明人： Steve Oh , Shaul Reuveny , Allen Chen , Jerry Kok Yen Chan , Zhiyong Zhang
IPC分类号： A61K35/32 , C12N5/077 , A61P19/00 , C12N5/0775
CPC分类号： C12N5/0606 , C12N2531/00 , C12N2533/50 , C12N2533/52 , C12N2533/54
摘要： The present application discloses a method of generating bone tissue in vivo comprising implanting cells into a human or animal at a location where bone growth is required, wherein the cells have been obtained by the in vitro suspension culture of mesenchymal stem cells attached to microcarriers.
摘要翻译：本申请公开了一种在体内产生骨组织的方法，包括在需要骨生长的位置将细胞植入人或动物中，其中通过体外悬浮培养获得连接于微载体的间充质干细胞。

2. 发明申请

US20110111498A1 Microcarriers for Stem Cell Culture 有权
公开(公告)号：US20110111498A1
公开(公告)日：2011-05-12
申请号：US12949172
申请日：2010-11-18
申请人： Steve Oh , Shaul Reuveny , Allen Chen
发明人： Steve Oh , Shaul Reuveny , Allen Chen
IPC分类号： C12N5/0789 , C12N5/0735
CPC分类号： C12N5/0606 , C12M23/20 , C12M25/16 , C12N2531/00 , C12N2533/50 , C12N2533/52 , C12N2533/54
摘要： We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.

3. 发明授权

US08691569B2 Microcarriers for stem cell culture 有权
公开(公告)号：US08691569B2
公开(公告)日：2014-04-08
申请号：US12917210
申请日：2010-11-01
申请人： Steve Oh , Shaul Reuveny , Allen Chen
发明人： Steve Oh , Shaul Reuveny , Allen Chen
IPC分类号： C12N5/07 , C12N5/02
CPC分类号： C12N5/0075 , C12N5/0606
摘要： We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.

4. 发明申请

US20120009645A1 CULTURE OF PLURIPOTENT AND MULTIPOTENT CELLS ON MICROCARRIERS 有权
标题翻译：微生物培养和多种细胞培养
公开(公告)号：US20120009645A1
公开(公告)日：2012-01-12
申请号：US13257674
申请日：2010-03-12
申请人： Steve Oh , Allen Chen , Andre Choo , Shaul Reuveny
发明人： Steve Oh , Allen Chen , Andre Choo , Shaul Reuveny
IPC分类号： C12N11/14 , C12N5/071 , C12N11/02 , C12N11/08 , C12N11/00 , C12N11/10
CPC分类号： C12N5/0606 , C12N5/0696 , C12N2501/727 , C12N2531/00
摘要： A method is disclosed for culturing pluripotent or multipotent cells in vitro, the method comprising attaching pluripotent or multipotent cells to a plurality of microcarriers to form microcarrier-cell complexes, and culturing the microcarrier-cell complexes in suspension culture in the presence of a ROCK inhibitor.
摘要翻译：公开了用于在体外培养多能细胞或多能细胞的方法，所述方法包括将多能或多潜能细胞连接到多个微载体上以形成微载体 - 细胞复合物，并在ROCK抑制剂存在下在悬浮培养物中培养微载体 - 细胞复合物。

5. 发明申请

US20110129919A1 Microcarriers for Stem Cell Culture 有权
标题翻译：干细胞培养的微载体
公开(公告)号：US20110129919A1
公开(公告)日：2011-06-02
申请号：US12917210
申请日：2010-11-01
申请人： Steve Oh , Shaul Reuveny , Allen Chen
发明人： Steve Oh , Shaul Reuveny , Allen Chen
IPC分类号： C12N5/071 , C12N5/0793 , C12N5/079
CPC分类号： C12N5/0075 , C12N5/0606
摘要： We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.
摘要翻译：我们公开了包含其上涂覆有基质并具有正电荷的颗粒的颗粒，该颗粒的尺寸允许附着于其上的灵长类动物或人类干细胞的聚集。颗粒可以包括具有50μm至400μm之间的最长尺寸（例如约200μm）的基本细长的圆柱形或棒状颗粒。它的横截面尺寸可以在20μm和30μm之间。该颗粒可以包括具有约20μm和约120μm之间，例如约65μm的尺寸的基本致密或球形的颗粒。我们还公开了增加灵长类动物或人类干细胞的方法，该方法包括：提供连接到第一和第二相应颗粒上的第一和第二灵长类动物或人类干细胞，使第一灵长类动物或人类干细胞接触第二灵长类动物或人类干细胞细胞以形成细胞聚集体并培养所述聚集体以使所述灵长类动物或人类干细胞传播至少一个通道。还公开了使用包被在基质胶或透明质酸中的微载体在长期悬浮培养物中繁殖人胚胎干细胞（hESC）的方法。我们还公开了一种区分干细胞的方法。

6. 发明申请

US20110294210A1 Microcarriers for Stem Cell Culture 有权
公开(公告)号：US20110294210A1
公开(公告)日：2011-12-01
申请号：US13210475
申请日：2011-08-16
申请人： Steve Oh , Marti Lecina , Andre Choo , Shaul Reuveny , Robert Zweigert , Allen Chen
发明人： Steve Oh , Marti Lecina , Andre Choo , Shaul Reuveny , Robert Zweigert , Allen Chen
IPC分类号： C12N5/071 , C12N5/074
CPC分类号： C12N5/0606 , C12N5/0062 , C12N5/0075 , C12N5/0647
摘要： We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.

7. 发明申请

US20110143433A1 Microcarriers for Stem Cell Culture 审中-公开
公开(公告)号：US20110143433A1
公开(公告)日：2011-06-16
申请号：US12917268
申请日：2010-11-01
申请人： Steve Oh , Shaul Reuveny , Allen Chen
发明人： Steve Oh , Shaul Reuveny , Allen Chen
IPC分类号： C12N5/0775
CPC分类号： C12N5/0606 , C12N5/0662 , C12N5/0696 , C12N2500/44 , C12N2500/84 , C12N2500/90 , C12N2501/115 , C12N2501/90 , C12N2501/91 , C12N2502/13 , C12N2531/00 , C12N2533/30 , C12N2533/32 , C12N2533/50 , C12N2533/52 , C12N2533/54 , C12N2533/78 , C12N2533/80 , C12N2533/90
摘要： We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.

8. 发明申请

US20110014693A1 Microcarriers For Stem Cell Culture 有权
标题翻译：微载体用于干细胞培养
公开(公告)号：US20110014693A1
公开(公告)日：2011-01-20
申请号：US12921599
申请日：2009-03-17
申请人： Steve Oh , Marti Lecina , Andre Choo , Shaul Reuveny , Robert Zweigert , Allen Chen
发明人： Steve Oh , Marti Lecina , Andre Choo , Shaul Reuveny , Robert Zweigert , Allen Chen
IPC分类号： C12N5/07 , C12N5/02
CPC分类号： C12N5/0606 , C12N5/0062 , C12N5/0075 , C12N5/0647
摘要： We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.
摘要翻译：我们公开了包含其上涂覆有基质并具有正电荷的颗粒的颗粒，该颗粒的尺寸允许附着于其上的灵长类动物或人类干细胞的聚集。颗粒可以包括具有50μm至400μm之间的最长尺寸（例如约200μm）的基本细长的圆柱形或棒状颗粒。它的横截面尺寸可以在20μm和30μm之间。该颗粒可以包括具有约20μm和约120μm之间，例如约65μm的尺寸的基本致密或球形的颗粒。我们还公开了增加灵长类动物或人类干细胞的方法，该方法包括：提供连接到第一和第二相应颗粒上的第一和第二灵长类动物或人类干细胞，使第一灵长类动物或人类干细胞接触第二灵长类动物或人类干细胞细胞以形成细胞聚集体并培养所述聚集体以使所述灵长类动物或人类干细胞传播至少一个通道。还公开了使用包被在基质胶或透明质酸中的微载体在长期悬浮培养物中繁殖人胚胎干细胞（hESC）的方法。我们还公开了一种区分干细胞的方法。

9. 发明授权

US08716018B2 Microcarriers for stem cell culture 有权
公开(公告)号：US08716018B2
公开(公告)日：2014-05-06
申请号：US12921599
申请日：2009-03-17
申请人： Steve Oh , Marti Lecina , Andre Choo , Shaul Reuveny , Robert Zweigert , Allen Chen
发明人： Steve Oh , Marti Lecina , Andre Choo , Shaul Reuveny , Robert Zweigert , Allen Chen
IPC分类号： C12N5/07 , C12N5/02
CPC分类号： C12N5/0606 , C12N5/0062 , C12N5/0075 , C12N5/0647
摘要： We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.

10. 发明申请

US20100093053A1 Microcarriers for Stem Cell Culture 审中-公开
标题翻译：干细胞培养的微载体
公开(公告)号：US20100093053A1
公开(公告)日：2010-04-15
申请号：US12497591
申请日：2009-07-03
申请人： Steve Oh , Marti Lecina , Andre Choo , Shaul Reuveny , Robert Zweigert , Allen Chen
发明人： Steve Oh , Marti Lecina , Andre Choo , Shaul Reuveny , Robert Zweigert , Allen Chen
IPC分类号： C12N11/14 , C12N5/02 , C12N11/00 , C12N11/12 , C12N11/10 , C12N11/02
CPC分类号： C12N5/0606 , C12N5/0062 , C12N5/0075 , C12N5/0647
摘要： We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.
摘要翻译：我们公开了包含其上涂覆有基质并具有正电荷的颗粒的颗粒，该颗粒的尺寸允许附着于其上的灵长类动物或人类干细胞的聚集。颗粒可以包括具有50μm至400μm之间的最长尺寸（例如约200μm）的基本细长的圆柱形或棒状颗粒。它的横截面尺寸可以在20μm和30μm之间。该颗粒可以包括具有约20μm和约120μm之间，例如约65μm的尺寸的基本致密或球形的颗粒。我们还公开了增加灵长类动物或人类干细胞的方法，该方法包括：提供连接到第一和第二相应颗粒上的第一和第二灵长类动物或人类干细胞，使第一灵长类动物或人类干细胞接触第二灵长类动物或人类干细胞细胞以形成细胞聚集体并培养所述聚集体以使所述灵长类动物或人类干细胞传播至少一个通道。还公开了使用包被在基质胶或透明质酸中的微载体在长期悬浮培养物中繁殖人胚胎干细胞（hESC）的方法。我们还公开了一种区分干细胞的方法。

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