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    • 4. 发明申请
    • METHOD OF SCREEING FOR PATHOGENS
    • 病原体筛选方法
    • US20100028938A1
    • 2010-02-04
    • US12533083
    • 2009-07-31
    • Alistair J. BrownStephen Dimmer
    • Alistair J. BrownStephen Dimmer
    • C12Q1/04
    • G01N33/56938
    • Disclosed is a method of screening for the presence of MRSA in a subject, the method comprising the steps of: (a) obtaining a suitable sample from the subject using a swab or other suitable sample collection device; (b) substantially immediately after performing step (a), contacting the swab or other sample collection device with an identification medium, said medium comprising: (i) nutrients to allow the growth of MRSA; (ii) at least one inhibitor substance to inhibit preferentially the growth of organisms other than MRSA; and (iii) a visual indicator substance which generates a visual indication of the growth of MRSA if present; (c) incubating the identification medium, typically whilst still in contact with the swab or other sample collection device, in conditions which are sufficient to cause the growth of MRSA; and, after sufficient time has elapsed; (d) inspecting the medium to determine the presence or absence of the visual indication of the growth of MRSA.
    • 公开了一种筛选受试者中MRSA存在的方法,该方法包括以下步骤:(a)使用拭子或其它合适的样品收集装置从受试者获得合适的样品; (b)基本上在执行步骤(a)之后立即将拭子或其它样品收集装置与识别介质接触,所述介质包括:(i)允许MRSA生长的营养物质; (ii)至少一种抑制物质以优先抑制MRSA以外的生物体的生长; 和(iii)视觉指示物质,如果存在则产生MRSA生长的视觉指示; (c)在足以引起MRSA生长的条件下,通常在与拭子或其他样品采集装置接触的同时孵育鉴定培养基; 经过足够的时间后, (d)检查介质以确定是否存在MRSA生长的视觉指示。