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    • 10. 发明授权
    • Aptamer sensor device
    • 适配器传感器设备
    • US09157115B2
    • 2015-10-13
    • US13961407
    • 2013-08-07
    • Sony Corporation
    • Michael HulkoNadejda KrastevaIngeborg HospachGabriele Nelles
    • C07H21/02C07H21/04A61K48/00C12Q1/68G01N33/53G01N33/542
    • C12Q1/6834G01N33/5308G01N33/542G01N2333/9005
    • The present invention relates to aptazyme sensor devices comprising, in addition to the aptamer, ribozyme and communication components, a competitive inhibitory component with a metal nanoparticle or a competitive inhibitory and signalling component with a metal nanoparticle and a label, such that the enzymatic activity of the ribozyme is inhibited as long as the inhibitory or the inhibitory and signalling component is bound to the substrate binding site of the ribozyme. The aptazyme sensor devices of the invention have increased shelf-life and are suitable for the parallel detection of different ligands by using an array of aptazyme sensor devices utilizing inhibitory and inhibitory and signalling components with different metal nanoparticles. The present invention furthermore relates to the post-synthetic chemical modification of the aptamer component for avoiding unspecific binding.
    • 本发明涉及适配酶传感器装置,除了适体,核酶和通讯组分外,还包括具有金属纳米颗粒的竞争性抑制组分或具有金属纳米颗粒和标记的竞争性抑制和信号传导成分,使得酶活性 只要抑制性或抑制性和信号传导成分与核酶的底物结合位点结合即可抑制核酶。 本发明的适体传感器装置具有增加的保质期,并且适用于通过使用利用具有不同金属纳米粒子的抑制性和抑制性和信号传导成分的适配子传感器装置阵列来平行检测不同的配体。 本发明还涉及用于避免非特异性结合的适配子组分的后合成化学修饰。