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1. 发明授权

US08716554B2 Plants resistant to cytoplasm-feeding parasites 有权
标题翻译：抗细胞质寄生虫的植物
公开(公告)号：US08716554B2
公开(公告)日：2014-05-06
申请号：US10568914
申请日：2004-08-22
申请人： Shulamit Michaeli , David Kenigsbuch , Orna Livneh , David Levy , Eli Khayat
发明人： Shulamit Michaeli , David Kenigsbuch , Orna Livneh , David Levy , Eli Khayat
IPC分类号： C12N15/01 , C12N15/82 , C12N15/87
CPC分类号： C12N15/8286 , C12N15/8282 , C12N15/8285 , Y02A40/162 , Y02A40/164
摘要： The present invention relates to transgenic plants resistant to parasites that their normal life cycle includes feeding on the plant cytoplasm, including insects, nematodes and fungi, wherein the plants are engineered to produce small interfering RNAs (siRNAs) capable of silencing a parasite specific gene. Particularly the parasite gene is a stage-specific gene, more particularly a gene involved in essential, early developmental stages of the parasite in or on the plant.
摘要翻译：本发明涉及抗寄生虫的转基因植物，它们的正常生命周期包括对植物细胞质（包括昆虫，线虫和真菌）进食，其中植物被工程化以产生能够沉默寄生虫特异性基因的小干扰RNA（siRNA）。特别地，寄生虫基因是阶段特异性基因，更特别地涉及参与植物中或植物上寄生虫的必需的早期发育阶段的基因。

2. 发明申请

US20070199100A1 Plants resistant to cytoplasm-feeding parasites 有权
标题翻译：抗细胞质寄生虫的植物
公开(公告)号：US20070199100A1
公开(公告)日：2007-08-23
申请号：US10568914
申请日：2004-08-22
申请人： Shulamit Michaeli , David Kenigsbuch , Orna Livneh , David Levy , Eli Khayat
发明人： Shulamit Michaeli , David Kenigsbuch , Orna Livneh , David Levy , Eli Khayat
IPC分类号： A01H1/00 , C07H21/04 , C12N15/82 , C12N5/04
CPC分类号： C12N15/8286 , C12N15/8282 , C12N15/8285 , Y02A40/162 , Y02A40/164
摘要： The present invention relates to transgenic plants resistant to parasites that their normal life cycle includes feeding on the plant cytoplasm, including insects, nematodes and fungi, wherein the plants are engineered to produce small interfering RNAs (siRNAs) capable of silencing a parasite specific gene. Particularly the parasite gene is a stage-specific gene, more particularly a gene involved in essential, early developmental stages of the parasite in or on the plant.
摘要翻译：本发明涉及抗寄生虫的转基因植物，它们的正常生命周期包括对植物细胞质（包括昆虫，线虫和真菌）进食，其中植物被工程化以产生能够沉默寄生虫特异性基因的小干扰RNA（siRNA）。特别地，寄生虫基因是阶段特异性基因，更特别地涉及参与植物中或植物上寄生虫的必需的早期发育阶段的基因。

3. 发明申请

US20090217411A1 SALT RESPONSIVE GENES USEFUL FOR GENERATING SALT RESISTANT TRANSGENIC PLANTS 有权
标题翻译：用于产生耐盐性转基因植物的盐胁迫基因
公开(公告)号：US20090217411A1
公开(公告)日：2009-08-27
申请号：US11574507
申请日：2005-09-01
申请人： Ada Zamir , Irena Gokhman , Eli Khayat , Nataly Vinikur , Orna Livneh , Avi Gabai
发明人： Ada Zamir , Irena Gokhman , Eli Khayat , Nataly Vinikur , Orna Livneh , Avi Gabai
IPC分类号： A01H5/00 , A01H5/10 , C12N15/82 , C12N15/29 , C12N5/10
CPC分类号： C12N15/8273 , C07K14/415 , C12N9/0006 , C12N9/0036 , C12N9/1217
摘要： The present invention relates to a transgenic plant comprising one or more plant cells transformed with exogenous nucleic acid encoding a Dunaliella salt-inducible or salt-responsive protein selected from the group consisting of elongation initiation factor 3 (eIF3), NADPH dependent quinone reductase (QOR), aldo-keto reductase (AKR), bifunctional aspartate kinase-homoserine reductase (AK-HSD) and mitochondrial import membrane translocase subunit (TIM9), or a fragment, homolog or variant thereof. The transgenic plant has increased tolerance to salt as compared to a corresponding non-transgenic plant. The present invention further relates to nucleic acids, vectors and constructs encoding the Dunaliella salt-inducible or salt-responsive proteins, and to a method of producing a transgenic plant having an increased tolerance to salt, a method of modifying a plant capacity to survive salt shock, and a method of modifying plant recovery after exposure to salt stress, by introducing the nucleic acids, constructs and/or vectors into one or more cells of the plant.
摘要翻译：本发明涉及一种转基因植物，其包含用外源核酸转化的一种或多种植物细胞，所述外源核酸编码选自延伸起始因子3（eIF3），NADPH依赖性醌还原酶（QOR）的杜氏盐藻诱导型或盐敏感性蛋白质），醛酮还原酶（AKR），双功能天冬氨酸激酶 - 高丝氨酸还原酶（AK-HSD）和线粒体进入膜转位酶亚基（TIM9）或其片段，同系物或变体。与相应的非转基因植物相比，转基因植物对盐的耐受性增加。本发明还涉及编码杜氏盐藻诱导型或盐反应性蛋白质的核酸，载体和构建体，以及生产对盐耐受性增加的转基因植物的方法，改变植物生存能力的方法休克，以及通过将核酸，构建体和/或载体引入植物的一个或多个细胞中而在暴露于盐胁迫后修饰植物恢复的方法。

4. 发明申请

US20090044290A1 SALT RESISTANT TRANSGENIC PLANTS 有权
标题翻译：耐盐转基因植物
公开(公告)号：US20090044290A1
公开(公告)日：2009-02-12
申请号：US11574506
申请日：2005-09-01
申请人： Uri Pick , Orna Livneh , Eli Khayat , Rina Walther , Avi Gabai
发明人： Uri Pick , Orna Livneh , Eli Khayat , Rina Walther , Avi Gabai
IPC分类号： C12N15/52 , A01H5/00 , C12N15/82
CPC分类号： C12N15/8273 , C12N9/14
摘要： The present invention provides transgenic plants transformed with exogenous nucleic acid encoding a Dunaliella plasma membrane (PM)-ATPase. The transgenic plant has increased tolerance to salt as compared to a corresponding non-transgenic plant. The present invention also provides nucleic acids encoding a chimeric PM-ATPase, which comprise a first portion encoding a plant PM-ATPase or a fragment thereof, and a second portion encoding a Dunaliella PM-ATPase or a fragment thereof. The present invention also discloses a method of producing a transgenic plant having an increased tolerance to salt as compared to a corresponding non-transgenic plant, a method of modifying a plant capacity to survive salt shock, and a method of modifying plant recovery after exposure to salt stress, by introducing into one or more cells of a plant an exogenous nucleic acid encoding a Dunaliella PM-ATPase. Also provided by the present invention are plant cells comprising an exogenous nucleic acid encoding a Dunaliella PM-ATPase, and plant seeds and progeny obtained from the transgenic plants.
摘要翻译：本发明提供了用编码杜氏藻质粒（PM）-ATPase的外源核酸转化的转基因植物。与相应的非转基因植物相比，转基因植物对盐的耐受性增加。本发明还提供编码嵌合PM-ATP酶的核酸，其包含编码植物PM-ATP酶或其片段的第一部分，以及编码杜氏藻属PM-ATP酶或其片段的第二部分。本发明还公开了一种生产与相应的非转基因植物相比具有增加的耐盐性的转基因植物的方法，改变植物体内耐盐胁迫的能力的方法，以及在暴露于盐胁迫，通过向植物的一个或多个细胞中引入编码杜氏藻属PM-ATP酶的外源核酸。本发明还提供了包含编码杜氏藻属PM-ATP酶的外源核酸和从转基因植物获得的植物种子和后代的植物细胞。

5. 发明授权

US08802931B2 Salt resistant transgenic plants 有权
标题翻译：耐盐转基因植物
公开(公告)号：US08802931B2
公开(公告)日：2014-08-12
申请号：US11574506
申请日：2005-09-01
申请人： Uri Pick , Orna Livneh , Eli Khayat , Rina Walther , Avi Gabai
发明人： Uri Pick , Orna Livneh , Eli Khayat , Rina Walther , Avi Gabai
IPC分类号： C12N15/05 , C12N15/01 , C12N15/11 , C12N15/63 , C12N15/82 , C12N1/12 , C07H21/04 , C07K14/405 , C12N15/52
CPC分类号： C12N15/8273 , C12N9/14
摘要： The present invention provides transgenic plants transformed with exogenous nucleic acid encoding a Dunaliella plasma membrane (PM)-ATPase. The transgenic plant has increased tolerance to salt as compared to a corresponding non-transgenic plant. The present invention also provides nucleic acids encoding a chimeric PM-ATPase, which comprise a first portion encoding a plant PM-ATPase or a fragment thereof, and a second portion encoding a Dunaliella PM-ATPase or a fragment thereof. The present invention also discloses a method of producing a transgenic plant having an increased tolerance to salt as compared to a corresponding non-transgenic plant, a method of modifying a plant capacity to survive salt shock, and a method of modifying plant recovery after exposure to salt stress, by introducing into one or more cells of a plant an exogenous nucleic acid encoding a Dunaliella PM-ATPase. Also provided by the present invention are plant cells comprising an exogenous nucleic acid encoding a Dunaliella PM-ATPase, and plant seeds and progeny obtained from the transgenic plants.
摘要翻译：本发明提供了用编码杜氏藻质粒（PM）-ATPase的外源核酸转化的转基因植物。与相应的非转基因植物相比，转基因植物对盐的耐受性增加。本发明还提供编码嵌合PM-ATP酶的核酸，其包含编码植物PM-ATP酶或其片段的第一部分，以及编码杜氏藻属PM-ATP酶或其片段的第二部分。本发明还公开了一种生产与相应的非转基因植物相比具有增加的耐盐性的转基因植物的方法，改变植物体内耐盐胁迫的能力的方法，以及在暴露于盐胁迫，通过向植物的一个或多个细胞中引入编码杜氏藻属PM-ATP酶的外源核酸。本发明还提供了包含编码杜氏藻属PM-ATP酶的外源核酸和从转基因植物获得的植物种子和后代的植物细胞。

6. 发明授权

US07897840B2 Salt responsive genes useful for generating salt resistant transgenic plants 有权
标题翻译：盐响应基因可用于产生耐盐转基因植物
公开(公告)号：US07897840B2
公开(公告)日：2011-03-01
申请号：US11574507
申请日：2005-09-01
申请人： Ada Zamir , Irena Gokhman , Eli Khayat , Nataly Vinikur , Orna Livneh , Avi Gabai
发明人： Ada Zamir , Irena Gokhman , Eli Khayat , Nataly Vinikur , Orna Livneh , Avi Gabai
IPC分类号： A01H5/00 , C12N15/82 , C12N5/14 , C07H21/00
CPC分类号： C12N15/8273 , C07K14/415 , C12N9/0006 , C12N9/0036 , C12N9/1217
摘要： The present invention provides transgenic plants transformed with exogenous nucleic acid encoding a Dunaliella salt-inducible or salt-responsive protein selected from the group consisting of eukaryotic initiation factor 3 (eIF3) subunit, NADPH dependent quinone reductase (QOR), aldo-keto reductase (AKR), bifunctional aspartate kinase-homoserine reductase (AK-HSD) and mitochondrial import membrane translocase subunit (TIM9), or a fragment, homolog or variant thereof. The transgenic plants have increased tolerance to salt as compared to corresponding non-transgenic plants. The present invention further provides nucleic acids, constructs and vectors encoding the Dunaliella salt-inducible or otherwise salt-responsive proteins, and to a method of producing transgenic plants having an increased tolerance to salt, a method of modifying plant capacity to survive salt shock, and a method of modifying plant recovery after exposure to salt stress, by introducing the nucleic acids, constructs and/or vectors into one or more cells of the plant.
摘要翻译：本发明提供用外源核酸转化的转基因植物，所述外源核酸编码选自真核起始因子3（eIF3）亚基，NADPH依赖性醌还原酶（QOR），醛酮还原酶（DOR） AKR），双功能天冬氨酸激酶 - 高丝氨酸还原酶（AK-HSD）和线粒体进入膜转位酶亚基（TIM9）或其片段，同系物或变体。与相应的非转基因植物相比，转基因植物对盐的耐受性增加。本发明还提供了编码杜氏盐杆菌盐诱导型或其他盐敏感性蛋白质的核酸，构建体和载体，以及生产对盐耐受性增加的转基因植物的方法，改变植物生存能力以进行盐胁迫的方法，以及通过将核酸，构建体和/或载体引入植物的一个或多个细胞中而在暴露于盐胁迫后修饰植物恢复的方法。

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