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1. 发明授权

US6066487A Method for cloning and expression of BsrFI restriction endonuclease in E. coli 有权
标题翻译：在大肠杆菌中克隆和表达BsrFI限制性内切核酸酶的方法
公开(公告)号：US6066487A
公开(公告)日：2000-05-23
申请号：US307621
申请日：1999-05-07
申请人： Shuang-yong Xu , Jian-Ping Xiao
发明人： Shuang-yong Xu , Jian-Ping Xiao
IPC分类号： C12N9/22 , C12N15/55
CPC分类号： C12N9/22
摘要： BsrFI restriction enzyme was purified from Bacillus stearothermophilus to near homogeneity. The protein was sequenced to obtain its N-terminus amino acid sequence. A set of denegerate primers were synthesized based on the aa sequence. The first 18 codons encoding BsrFI restriction endonuclease (bsrFIR) was amplified by PCR and its coding sequence was obtained. The methylase selection method was used to clone BsrFI methylase gene (bsrFIM). Two clones were found to be resistant to BsrFI digstion. The entire insert in one clone was sequenced and the insert encodes the BsrFI methylase (M. BsrFI). In addition, a small truncated open reading frame adjacent to the methylase gene has homology to Cfr10I restriciton endonuclease in a BlastX homology search in Genbank database. BsrFI and Cfr10I are isoschizomer that recognizes and cleaves 5'R CCGGY3'. Two primers were used to amplify the bsrFIR gene, The forward primer is a degenerate primer designed from the N-terminus aa sequence and the reverse primer is the bona fide sequence derived from the BsrFI methylase.sup.+ clone. The bsrFIR gene was amplified by PCR, ligated into a T7 expression vector pET21at and the ligated DNA was transformed into premodified cells ER2566 [pLG339-BsrFIM]. The final expression strain is ER2566 [pLG339-BsrFIM, pET21at-BsrFIR]. Recombinant BsrFI activity was detected in E. coli cell extract. BsrFI is cloned from a thermophile Bacillus stearothermophilus. Thus, BsrFI a thermostable enzyme and it is active at 37.degree. C. to 65.degree. C.
摘要翻译：将BsrFI限制性酶从嗜热脂肪芽孢杆菌纯化至接近同质性。对蛋白进行测序以获得其N-末端氨基酸序列。基于aa序列合成了一组脱壳引物。通过PCR扩增编码BsrFI限制性内切核酸酶（bsrFIR）的前18个密码子，获得编码序列。甲基化酶选择法用于克隆BsrFI甲基化酶基因（bsrFIM）。发现两个克隆对BsrFI突变具有抗性。对一个克隆中的整个插入片段进行测序，插入序列编码BsrFI甲基化酶（M.BsrFI）。此外，在Genbank数据库中的BlastX同源性搜索中，与甲基化酶基因相邻的小截断的开放阅读框与Cfr10I限制性内切核酸酶具有同源性。 BsrFI和Cfr10I是识别并切割5'R + E，cir + EE CCGGY3'的异构体。使用两个引物来扩增bsrFIR基因。正向引物是从N末端aa序列设计的简并引物，反向引物是来自BsrFI甲基化酶+克隆的真正序列。通过PCR扩增bsrFIR基因，连接到T7表达载体pET21at中，将连接的DNA转化到预变性细胞ER2566 [pLG339-BsrFIM]中。最终的表达菌株是ER2566 [pLG339-BsrFIM，pET21at-BsrFIR]。在大肠杆菌细胞提取物中检测到重组BsrFI活性。从嗜热芽孢杆菌嗜热脂肪芽孢杆菌克隆BsrFI。因此，BsrFI是一种热稳定酶，在37℃至65℃下是有活性的。

2. 发明授权

US5721126A Method for cloning and producing the SCaI restriction endonuclease in E. coli 失效
标题翻译：在大肠杆菌中克隆和产生SCaI限制性内切核酸酶的方法
公开(公告)号：US5721126A
公开(公告)日：1998-02-24
申请号：US569806
申请日：1995-12-08
申请人： Shuang-Yong Xu , Jian-Ping Xiao
发明人： Shuang-Yong Xu , Jian-Ping Xiao
IPC分类号： C12N15/09 , C07H21/04 , C12N1/21 , C12N9/10 , C12N9/16 , C12N9/22 , C12N15/00 , C12N15/54 , C12N15/55 , C12R1/19 , C12R1/465 , C12N15/70
CPC分类号： C12N9/22 , C12N9/1007
摘要： The present invention relates to isolated DNA coding for the restriction endonuclease SCaI as well as to a method for cloning methylase genes from Streptomyces into E. coli by a modification of the methylase selection method. At first, the standard methylase gene selection method was tried to clone the SCaI methylase gene using a high-copy-number cloning vector pUC19 during library construction. The SCaI methylase gene was refractory to cloning by using pUC19, presumably due to the poor expression of the SCaI methylase gene in E. coli. If the SCaI methylase is not efficiently expressed in E. coli, the SCaI sites on the plasmid will not be sufficiently modified by the methylase. As a consequence, the plasmid will be cleaved and lost in the plasmid library after SCaI endonuclease challenge. Since the standard methylase selection did not work, the "endo-blue method" was tried to clone the SCaI endonuclease gene. Nineteen blue colonies were identified, but none of them yielded any detectable SCaI endonuclease activity. The SCaI endonuclease gene was first cloned by inverse PCR using primers that annealed to the end of the SCaI methylase gene. In order to increase the SCaI endonuclease expression in E. coli, an optimal ribosome binding site and spacing were engineered in front of the ATG start codon and the gene was inserted into expression vector pRRS.
摘要翻译：本发明涉及编码限制性内切核酸酶SCaI的分离的DNA以及通过甲基转移酶选择方法的修饰将来自链霉菌的甲基化酶基因克隆到大肠杆菌中的方法。首先，在文库构建期间，使用高拷贝数克隆载体pUC19，尝试使用标准甲基化酶基因选择方法克隆SCaI甲基化酶基因。 SCaI甲基化酶基因通过使用pUC19进行克隆难以进行，大概是由于SCaI甲基化酶基因在大肠杆菌中的表达差。如果SCaI甲基化酶不能在大肠杆菌中有效表达，则质粒上的SCaI位点将不会被甲基化酶充分修饰。因此，在SCaI核酸内切酶攻击后，质粒将被切割并丢失在质粒文库中。由于标准甲基化酶选择不起作用，因此试图克隆SCaI内切核酸酶基因的“内 - 蓝法”。鉴定了十九个蓝色菌落，但没有一个产生任何可检测的SCaI核酸内切酶活性。首先通过使用退火到SCaI甲基化酶基因末端的引物通过反向PCR克隆SCaI核酸内切酶基因。为了增加大肠杆菌中的SCaI核酸内切酶表达，在ATG起始密码子前方设计了最佳的核糖体结合位点和间隔，并将该基因插入到表达载体pRRS中。

3. 发明授权

US08282417B2 Electrical connector with cooperating upper and lower shield wings 有权
标题翻译：电气连接器，配合上，下屏蔽翼
公开(公告)号：US08282417B2
公开(公告)日：2012-10-09
申请号：US12951097
申请日：2010-11-22
申请人： Jian-Ping Xiao
发明人： Jian-Ping Xiao
IPC分类号： H01R13/648
CPC分类号： H01R13/648
摘要： An electrical connector (100) includes a dielectric housing (1) having a base portion (11) and a tongue portion (12) extending from the base portion; a number of terminals (2) received in the housing; and an upper shield (31) and a lower shield (32) enclosing the insulative housing. The upper shield includes a main cover (310) located above the insulative housing and a pair of wing portions (312) extending oppositely, laterally relative to the main cover. The lower shield includes a main plate (320) located below the insulative housing and a pair of wings (321) extending oppositely, laterally relative to the main plate. One of the wing portion and corresponding wing defines a closed slot (3122) and the other one of the wing portion and the corresponding wing integrally, interiorly fits in the closed slot.
摘要翻译：电连接器（100）包括具有基部（11）和从基部延伸的舌部（12）的电介质壳体（1）在房屋中接收的多个终端（2）; 以及围绕所述绝缘壳体的上屏蔽件（31）和下屏蔽件（32）。上屏蔽包括位于绝缘壳体上方的主盖（310）和相对于主盖相对地相对延伸的一对翼部（312）。下屏蔽包括位于绝缘壳体下方的主板（320）和相对于主板相对地横向延伸的一对翼（321）。翼部分和对应的翼中的一个限定了封闭的狭槽（3122），并且翼部分和对应翼片中的另一个一体地内部配合在封闭的狭槽中。

4. 发明授权

US07549896B2 Low profile receptacle connector straddle-mounted on the PCB 有权
标题翻译：薄板插座连接器跨接在PCB上
公开(公告)号：US07549896B2
公开(公告)日：2009-06-23
申请号：US12157782
申请日：2008-06-12
申请人： Han-Ming Zhang , Tsuneki Watanabe , Jian-Ping Xiao
发明人： Han-Ming Zhang , Tsuneki Watanabe , Jian-Ping Xiao
IPC分类号： H01R13/648
CPC分类号： H01R12/707 , H01R12/57 , H01R12/716 , H01R13/504 , H01R13/6581
摘要： A receptacle connector (100) includes a metal shield (3) having the cover (31) and an opposite base (32). The cover (31) includes a pair of top lateral wings (312). The base includes a pair of bottom lateral wings (322) for mating with the respective top lateral wings (312). The top lateral wing (312) defines a first portion of a whole lateral wing board, while the bottom lateral wing (322) defines a second portion adapted to contribute towards completing the whole lateral wing board. Thus, the metal shield (3) is integrated by the cover (31) and the base (32), each of which is molded by a single sheet.
摘要翻译：插座连接器（100）包括具有盖（31）和相对的底座（32）的金属屏蔽（3）。盖（31）包括一对顶侧翼（312）。基座包括一对用于与相应的顶侧翼（312）配合的底侧翼（322）。顶侧翼（312）限定整个侧翼板的第一部分，而底侧翼（322）限定适于完成整个侧翼板的第二部分。因此，金属屏蔽（3）由盖（31）和基座（32）整合，每个都由单片模制。

5. 发明授权

US08721347B2 RF receptacle connector having central conductor firmly retained with insulative housing 有权
标题翻译： RF插座连接器具有牢固地保持有绝缘壳体的中心导体
公开(公告)号：US08721347B2
公开(公告)日：2014-05-13
申请号：US13429682
申请日：2012-03-26
申请人： Jian-Ping Xiao , Ming-Lun Kuo
发明人： Jian-Ping Xiao , Ming-Lun Kuo
IPC分类号： H01R12/00
CPC分类号： H01R24/50 , H01R2103/00
摘要： An RF connector (100) for receiving a mating connector along a mating direction, includes an insulative housing (1), an outer conductor (3) retained with the insulative housing, and a central conductor (2) retained with the insulative housing. The outer conductor includes a tubular section (31) defining an axial line along the mating direction and a number of leg sections (32, 33) extending outwardly from a bottom of the tubular section. The central conductor includes a contact section (22) positioned within the tubular section along the mating direction, a radial section (21) extending outwardly from a bottom of the contact section along a radial direction perpendicular to the mating direction, and an extension section (24) extending out of the insulative housing. The extension section is connected with the radial section via a declined connection portion (23). The insulative housing extends below the radial section.
摘要翻译：用于沿配合方向接收配合连接器的RF连接器（100）包括绝缘壳体（1），与绝缘壳体保持的外导体（3）和与绝缘壳体保持的中心导体（2）。外部导体包括限定沿着配合方向的轴线的管状部分（31）和从管状部分的底部向外延伸的多个腿部部分（32,33）。中心导体包括沿着配合方向定位在管状部分内的接触部分（22），沿着垂直于配合方向的径向方向从接触部分的底部向外延伸的径向部分（21）和延伸部分 24）延伸出绝缘外壳。延伸部分经由倾斜连接部分（23）与径向部分连接。绝缘外壳延伸到径向部分之下。

6. 发明授权

US08142226B2 Electrical connector with improved notch structure to separate large and small receiving cavities arranged side by side 有权
标题翻译：具有改进的凹口结构的电连接器，用于分离并排布置的大小容纳腔
公开(公告)号：US08142226B2
公开(公告)日：2012-03-27
申请号：US13218457
申请日：2011-08-26
申请人： Jian-Ping Xiao , Tsuneki Watanabe
发明人： Jian-Ping Xiao , Tsuneki Watanabe
IPC分类号： H01R13/648
CPC分类号： H01R27/02 , H01R13/6583 , H01R13/6593 , H01R13/6598
摘要： An electrical connector (100) includes an insulative body (1) having a wide tongue (123) and a narrow tongue (121) split by a gap (124) therebetween. A metallic shell (32) includes a front pocket (320) defining a large receiving cavity (327) enclosing the wide tongue (123), a small receiving cavity (325) enclosing the narrow tongue (121), and a non-circumferentially enclosed notch structure (33) which protrudes into the gap (124) so as to form the large receiving cavity (327) and the small receiving cavity (325). Besides, the notch structure (33) defines a notch (330) opened to an exterior from a bottom side thereof. First and second sets of contacts (211, 212) are located in the wide tongue and the narrow tongue, respectively, and the first contacts (211) are compatible to version 2.0 Micro Universal Serial Bus.
摘要翻译：电连接器（100）包括具有宽舌状物（123）的绝缘体（1）和由它们之间的间隙（124）分开的窄舌片（121）。金属外壳（32）包括限定包围宽榫舌（123）的大容纳腔（327）的前袋（320），包围窄榫舌（121）的小容纳腔（325），以及非周向封闭凹口结构（33），其突出到所述间隙（124）中以形成所述大容纳腔（327）和所述小容纳腔（325）。此外，切口结构（33）限定从其底侧向外部开口的凹口（330）。第一和第二组触点（211,212）分别位于宽舌头和窄舌部中，并且第一触点（211）与2.0微型通用串行总线兼容。

7. 发明授权

US08011960B2 Electrical connector assembly with assembled main shell and sub-shell 有权
标题翻译：具有组装主壳和子壳的电连接器组件
公开(公告)号：US08011960B2
公开(公告)日：2011-09-06
申请号：US12611120
申请日：2009-11-03
申请人： Jian-Ping Xiao , Tsuneki Watanabe
发明人： Jian-Ping Xiao , Tsuneki Watanabe
IPC分类号： H01R13/648
CPC分类号： H01R27/02 , H01R13/6583 , H01R13/6593 , H01R13/6598
摘要： An electrical connector assembly (100) comprises an insulative body (1) integrally including a forward part (12) and a backward part (11). A main shell (32) includes a front pocket (320) enclosing the forward part (12), and a back segment (322) covering an upper portion of the backward part (11) of the body (1). A sub-shell (31) is assembled with the main shell (32), and includes a lower piece (316) covering a lower portion of the backward part (11). Therefore, the main shell (32), the sub-shell (31) are assembled with each other to provide a good shielding enclosure for the insulative body (1), where the contact terminals (2) are held therein.
摘要翻译：电连接器组件（100）包括一体地包括前部（12）和后部（11）的绝缘体（1）。主壳体（32）包括封闭前部（12）的前槽（320）和覆盖主体（1）的后部（11）的上部的后段（322）。子壳（31）与主壳体（32）组装，并且包括覆盖后部（11）的下部的下部件（316）。因此，主壳体（32）和副壳体（31）彼此组装，为绝缘体（1）提供良好的屏蔽外壳，其中接触端子（2）保持在其中。

8. 发明授权

US08303344B2 Electrical connector with improved notch structure to separate large and small receiving cavities arranged side by side 有权
公开(公告)号：US08303344B2
公开(公告)日：2012-11-06
申请号：US13430711
申请日：2012-03-27
申请人： Jian-Ping Xiao , Tsuneki Watanabe
发明人： Jian-Ping Xiao , Tsuneki Watanabe
IPC分类号： H01R13/648
CPC分类号： H01R27/02 , H01R13/6583 , H01R13/6593 , H01R13/6598
摘要： An electrical connector (100) includes an insulative body (1) having a wide tongue (123) and a narrow tongue (121) split by a gap (124) therebetween. A metallic shell (32) includes a front pocket (320) defining a large receiving cavity (327) enclosing the wide tongue (123), a small receiving cavity (325) enclosing the narrow tongue (121), and a non-circumferentially enclosed notch structure (33) which protrudes into the gap (124) so as to form the large receiving cavity (327) and the small receiving cavity (325). Besides, the notch structure (33) defines a notch (330) opened to an exterior from a bottom side thereof. First and second sets of contacts (211, 212) are located in the wide tongue and the narrow tongue, respectively, and the first contacts (211) are compatible to version 2.0 Micro Universal Serial Bus.

9. 发明申请

US20080311793A1 Low profile receptacle connector straddle-mounded on the PCB 有权
标题翻译：薄型插座连接器跨接在PCB上
公开(公告)号：US20080311793A1
公开(公告)日：2008-12-18
申请号：US12157782
申请日：2008-06-12
申请人： Han-Ming Zhang , Tsuneki Watanabe , Jian-Ping Xiao
发明人： Han-Ming Zhang , Tsuneki Watanabe , Jian-Ping Xiao
IPC分类号： H01R13/648
CPC分类号： H01R12/707 , H01R12/57 , H01R12/716 , H01R13/504 , H01R13/6581
摘要： A receptacle connector (100) includes a metal shield (3) having the cover (31) and an opposite base (32). The cover (31) includes a pair of top lateral wings (312). The base includes a pair of bottom lateral wings (322) for mating with the respective top lateral wings (312). The top lateral wing (312) defines a first portion of a whole lateral wing board, while the bottom lateral wing (322) defines a second portion adapted to contribute towards completing the whole lateral wing board. Thus, the metal shield (3) is integrated by the cover (31) and the base (32), each of which is molded by a single sheet.
摘要翻译：插座连接器（100）包括具有盖（31）和相对的底座（32）的金属屏蔽（3）。盖（31）包括一对顶侧翼（312）。基座包括一对用于与相应的顶侧翼（312）配合的底侧翼（322）。顶侧翼（312）限定整个侧翼板的第一部分，而底侧翼（322）限定适于完成整个侧翼板的第二部分。因此，金属屏蔽（3）由盖（31）和基座（32）整合，每个都由单片模制。

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