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    • 1. 发明申请
    • Biochip detection device and detection method therof
    • 生物芯片检测装置及检测方法
    • US20090317915A1
    • 2009-12-24
    • US12453700
    • 2009-05-20
    • Shu-Jen HanJoseph Shu-Tzu Han
    • Shu-Jen HanJoseph Shu-Tzu Han
    • G01N27/00
    • G01R33/093B82Y25/00G01R33/1269
    • The present invention provides a biochip detection device and a detection method thereof. The detection device includes a detection circuit and a biochip containing a plurality of sensor modules. Each sensor modules includes a plurality of giant magnetoresistive biosensors. The detection circuit is arranged to have an end of each biosensor and an end of each of reference sensors respectively connected to first and second voltage sources, whereby current variation induced in each biosensor can be added together. The detection method includes the steps of providing the above described biochip; carrying out surface functionalization on the biosensors; spotting surfaces of the biosensors with probe molecules corresponding to target molecules to complete molecule immobilization; applying a purified sample to the biochip so that target molecules existing in the sample bind to the probe molecules on the surfaces of the biosensors; applying detecting molecules that are combined with magnetic nano-particles to the biochip in such a way that the detecting molecules are complementary to and thus bound to the target molecules; and using the above mentioned detection circuit to supply an output of a detection current of the biosensors so that observation of variation thereof is made to determine existence of the target molecules.
    • 本发明提供一种生物芯片检测装置及其检测方法。 检测装置包括检测电路和包含多个传感器模块的生物芯片。 每个传感器模块包括多个巨磁阻生物传感器。 检测电路设置成具有每个生物传感器的端部,并且每个参考传感器的端部分别连接到第一和第二电压源,由此可以将每个生物传感器中引起的电流变化加在一起。 检测方法包括提供上述生物芯片的步骤; 在生物传感器上进行表面官能化; 用对应于靶分子的探针分子发射生物传感器的表面以完成分子固定化; 将纯化的样品施加到生物芯片上,使得存在于样品中的靶分子与生物传感器表面上的探针分子结合; 将与磁性纳米颗粒结合的检测分子施加到生物芯片上,使得检测分子与靶分子互补并因此结合到靶分子; 并且使用上述检测电路来提供生物传感器的检测电流的输出,使得观察其变化来确定目标分子的存在。