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1. 发明申请

US20130244239A1 NUCLEIC ACID CONSTRUCT AND COMPLEX FORMATION METHOD AND SCREENING METHOD USING THE SAME 审中-公开
标题翻译：核酸结构和复合形成方法及使用该方法的筛选方法
公开(公告)号：US20130244239A1
公开(公告)日：2013-09-19
申请号：US13638447
申请日：2011-03-31
申请人： Shotaro Tsuji , Makiko Tsuji , Takashi Ohtsu
发明人： Shotaro Tsuji , Makiko Tsuji , Takashi Ohtsu
IPC分类号： G01N33/68 , C07K19/00
CPC分类号： G01N33/68 , C07K19/00 , C12N15/10 , C12N15/1048 , C12N15/1062 , C12N15/1075 , C12N15/111 , C12N2310/16 , C12N2310/3519 , C12N2320/12 , C12Q1/6811 , G01N2500/04 , C12Q2525/205 , C12Q2563/131 , C12Q2525/179
摘要： The present invention is to provide a new detection method that is superior in detection sensitivity and allows a simple operation. A nucleic acid construct that includes a cloning region, an encoding nucleic acid of a peptide tag, an encoding nucleic acid of an aptamer that is bindable to the peptide tag is used. By inserting the encoding nucleic acid of arbitrary peptide into the cloning region of the nucleic acid construct, the nucleic acid construct is expressed in vivo. Thereby, a complex of a fusion transcript having the base sequence that includes the encoding nucleic acid of arbitrary peptide, the encoding nucleic acid of a peptide tag, and the encoding nucleic acid of the aptamer and a fusion translation that includes the arbitrary peptide and the peptide tag is formed. By bringing the complex into contact with a target and recovering the complex that is bound to the target, the peptide that is bindable to a target and its encoding nucleic acid can be identified from the transcript of the encoding nucleic acid of arbitrary peptide in the complex.
摘要翻译：本发明提供一种检测灵敏度优异且能够简单操作的新检测方法。使用包含克隆区，肽标签的编码核酸，可与肽标签结合的适体的编码核酸的核酸构建体。通过将任意肽的编码核酸插入到核酸构建体的克隆区中，核酸构建体在体内表达。由此，具有包含任意肽的编码核酸，肽标签的编码核酸和适配子的编码核酸的碱基序列的融合转录物的复合物和包含任意肽的融合翻译和形成肽标签。通过使复合物与靶接触并回收与靶结合的复合物，可以从复合物中任意肽的编码核酸的转录物中鉴定可与靶标结合的肽及其编码核酸。

2. 发明申请

US20140227724A1 NUCLEIC ACID CONSTRUCT FOR USE IN SCREENING FOR PEPTIDE ANTIBODY, AND SCREENING METHOD USING SAME 审中-公开
标题翻译：用于肽抗体筛选的核酸构建体及使用其的筛选方法
公开(公告)号：US20140227724A1
公开(公告)日：2014-08-14
申请号：US14346221
申请日：2012-08-10
申请人： Shotaro Tsuji , Makiko Tsuji
发明人： Shotaro Tsuji , Makiko Tsuji
IPC分类号： G01N33/53
CPC分类号： G01N33/5308 , C07K2319/21 , C12N15/1075 , C12N15/115 , C12N2310/16 , C12N2310/3519
摘要： The present invention provides a novel tool for simply screening a candidate molecule for an antibody and a method for screening a candidate molecule for an antibody using the tool. A nucleic acid construct includes: (x) an encoding nucleic acid of an antibody candidate, obtained by inserting an encoding nucleic acid of any peptide into an encoding nucleic acid of an antibody; (y) an encoding nucleic acid of a peptide tag; and (z) an encoding nucleic acid of an aptamer that is bindable to the peptide tag are used. When this nucleic acid construct is expressed, a complex of a fusion transcript of the encoding nucleic acids (x) to (z) and a fusion translation product of the encoding nucleic acids (x) and (y) is formed. When this complex and an antigen are brought into contact with each other, and the complex binding to the antigen is recovered, peptide that is bindable to the antigen and the encoding nucleic acid of the peptide can be identified from a transcript of an encoding nucleic acid of the any peptide in the complex.
摘要翻译：本发明提供了用于简单筛选抗体候选分子的新型工具和使用该工具筛选抗体候选分子的方法。核酸构建体包括：（x）通过将任何肽的编码核酸插入到抗体的编码核酸中而获得的抗体候选的编码核酸; （y）肽标签的编码核酸; 和（z）可以与肽标签结合的适体的编码核酸。当表达该核酸构建体时，形成编码核酸（x）至（z）的融合转录物与编码核酸（x）和（y）的融合翻译产物的复合物。当使该复合物和抗原相互接触，并且与抗原的复合物结合被回收时，可以从编码核酸的转录物鉴定可与抗原结合的肽和编码核酸的肽的复合物中的任何肽。

3. 发明申请

US20130123350A1 NUCLEIC ACID MOLECULE CAPABLE OF BINDING TO c-MET AND USE THEREOF 有权
标题翻译：可以与C-MET结合的核酸分子和其使用
公开(公告)号：US20130123350A1
公开(公告)日：2013-05-16
申请号：US13812190
申请日：2011-07-26
申请人： Naomi Hirabayashi , Shotaro Tsuji , Jou Akitomi , Shintarou Katou , Iwao Waga , Takashi Ohtsu
发明人： Naomi Hirabayashi , Shotaro Tsuji , Jou Akitomi , Shintarou Katou , Iwao Waga , Takashi Ohtsu
IPC分类号： C07H21/02
CPC分类号： C07H21/02 , A61K31/52 , A61K31/7105 , C12N15/115 , C12N2310/16 , C12Q1/6883 , C12Q2525/301 , C12Q2527/125 , A61K2300/00
摘要： The present invention provides a nucleic acid molecule capable of binding to c-Met as a substance that can be used for clarification of the pathogenic mechanism of diseases caused by c-Met, diagnosis and treatment of the diseases, and the like, and also the use thereof. The c-Met binding nucleic acid molecule of the present invention is any one of the following nucleic acid molecules (A1), (A2), (B1), and (B2).(A1) a nucleic acid molecule including the base sequence of any one of SEQ ID NOs: 1 to 38 (A2) a nucleic acid molecule that is capable of binding to c-Met and includes a base sequence obtained by substitution, deletion, addition, and/or insertion of one or more bases in the base sequence of any one of SEQ ID NOs: 1 to 38 (B1) a nucleic acid molecule including the base sequence of any one of SEQ ID NOs: 39 to 76 (B2) a nucleic acid molecule that is capable of binding to c-Met and includes a base sequence obtained by substitution, deletion, addition, and/or insertion of one or more bases in the base sequence of any one of SEQ ID NOs: 39 to 76
摘要翻译：本发明提供能够结合c-Met的核酸分子，作为能够用于澄清由c-Met，疾病的诊断和治疗等引起的疾病的致病机制的物质，以及使用它。本发明的c-Met结合核酸分子是以下核酸分子（A1），（A2），（B1）和（B2）中的任一种。（A1）包含SEQ ID NO：1至38（A2）中任一个的碱基序列的核酸分子，其能够结合c-Met并包含通过取代，缺失，加成获得的碱基序列，（SEQ ID NO：39）至（38）中的任何一个的碱基序列的核酸分子，和/或在SEQ ID NO：1至38（B1）中任一个的碱基序列中插入一个或多个碱基，能够结合c-Met的核酸分子，并且包含通过SEQ ID NO：39至76中任一个的碱基序列中的一个或多个碱基的取代，缺失，添加和/或插入获得的碱基序列

4. 发明授权

US09278108B2 HMGB1 binding nucleic acid molecule and applications thereof 有权
标题翻译： HMGB1结合核酸分子及其应用
公开(公告)号：US09278108B2
公开(公告)日：2016-03-08
申请号：US13383826
申请日：2010-07-16
申请人： Hiromi Takenaka , Jou Akitomi , Shintarou Katou , Shotaro Tsuji , Takashi Ohtsu , Iwao Waga
发明人： Hiromi Takenaka , Jou Akitomi , Shintarou Katou , Shotaro Tsuji , Takashi Ohtsu , Iwao Waga
IPC分类号： C12N15/115 , A61K31/7088 , A61K31/7105 , C07K14/47 , G01N33/68
CPC分类号： A61K31/7088 , A61K31/7105 , C07K14/4703 , C12N15/115 , C12N2310/16 , G01N33/6863
摘要： A nucleic acid molecule that can bind to HMGB1 protein and applications thereof are provided. A nucleic acid molecule having a dissociation constant for HMGB1 protein of 5×10−7 or less can be used as the nucleic acid molecule that can bind to HMGB1 protein. The HMGB1 binding nucleic acid molecule can bind to HMGB1 protein that is known to be a cause of diseases such as cancer and inflammation, and it is therefore possible to obtain an effect to prevent and an effect to treat such diseases by allowing the HMGB1 binding nucleic acid molecule to bind to HMGB1 protein in a living body.
摘要翻译：提供可结合HMGB1蛋白的核酸分子及其应用。具有5×10 -7以下的HMGB1蛋白的解离常数的核酸分子可以用作能结合HMGB1蛋白的核酸分子。 HMGB1结合核酸分子可以结合已知是诸如癌症和炎症等疾病的原因的HMGB1蛋白，因此可以通过使HMGB1结合核酸分子获得预防和治疗这些疾病的效果，酸分子结合活体中的HMGB1蛋白。

5. 发明授权

US08822667B2 Nucleic acid molecule capable of binding to c-Met and use thereof 有权
标题翻译：能够结合c-Met的核酸分子及其用途
公开(公告)号：US08822667B2
公开(公告)日：2014-09-02
申请号：US13812190
申请日：2011-07-26
申请人： Naomi Hirabayashi , Shotaro Tsuji , Jou Akitomi , Shintarou Katou , Iwao Waga , Takashi Ohtsu
发明人： Naomi Hirabayashi , Shotaro Tsuji , Jou Akitomi , Shintarou Katou , Iwao Waga , Takashi Ohtsu
IPC分类号： C07H21/04 , C07H21/02 , A61K48/00
CPC分类号： C07H21/02 , A61K31/52 , A61K31/7105 , C12N15/115 , C12N2310/16 , C12Q1/6883 , C12Q2525/301 , C12Q2527/125 , A61K2300/00
摘要： The present invention provides a nucleic acid molecule capable of binding to c-Met as a substance that can be used for clarification of the pathogenic mechanism of diseases caused by c-Met, diagnosis and treatment of the diseases, and the like, and also the use thereof. The c-Met binding nucleic acid molecule of the present invention is any one of the following nucleic acid molecules (A1), (A2), (B1), and (B2). (A1) a nucleic acid molecule including the base sequence of any one of SEQ ID NOs: 1 to 38 (A2) a nucleic acid molecule that is capable of binding to c-Met and includes a base sequence obtained by substitution, deletion, addition, and/or insertion of one or more bases in the base sequence of any one of SEQ ID NOs: 1 to 38 (B1) a nucleic acid molecule including the base sequence of any one of SEQ ID NOs: 39 to 76 (B2) a nucleic acid molecule that is capable of binding to c-Met and includes a base sequence obtained by substitution, deletion, addition, and/or insertion of one or more bases in the base sequence of any one of SEQ ID NOs: 39 to 76.
摘要翻译：本发明提供能够结合c-Met的核酸分子，作为能够用于澄清由c-Met，疾病的诊断和治疗等引起的疾病的致病机制的物质，以及使用它。本发明的c-Met结合核酸分子是以下核酸分子（A1），（A2），（B1）和（B2）中的任一种。（A1）包含SEQ ID NO：1至38（A2）中任一个的碱基序列的核酸分子，其能够结合c-Met并包含通过取代，缺失，加成获得的碱基序列，（SEQ ID NO：39）至（38）中的任何一个的碱基序列的核酸分子，和/或在SEQ ID NO：1至38（B1）中任一个的碱基序列中插入一个或多个碱基，能够结合c-Met的核酸分子，并且包含通过SEQ ID NO：39至76中任一个的碱基序列中的一个或多个碱基的取代，缺失，添加和/或插入获得的碱基序列。

6. 发明申请

US20130102480A1 PREDICTION DEVICE, PREDICTION METHOD, PROGRAM, AND RECORDING MEDIUM 有权
标题翻译：预测装置，预测方法，程序和记录介质
公开(公告)号：US20130102480A1
公开(公告)日：2013-04-25
申请号：US13807876
申请日：2011-07-02
申请人： Jou Akitomi , Shintarou Katou , Shotaro Tsuji , Takashi Ohtsu , Iwao Waga
发明人： Jou Akitomi , Shintarou Katou , Shotaro Tsuji , Takashi Ohtsu , Iwao Waga
IPC分类号： G06F19/16
CPC分类号： G06F19/16 , C12N15/115 , C12N2310/16 , G06F19/18
摘要： The present invention provides a prediction device, a prediction method, a program, and a recording medium, with which whether or not desired aptamer sequences are enriched can be predicted easily. The prediction device of the present invention 10 includes an input unit 11, a calculation unit 12, and a prediction unit 13. The input unit 11 is a unit through which sequence information on a target aptamer sequence group including selected aptamers in a target pool and a reference aptamer sequence group including reference aptamer sequences are inputted. The calculation unit 12 calculates the free energy of the target aptamer sequence group and the free energy of the reference aptamer sequence group. The prediction unit 13 compares the free energy of these sequence groups, and predicts that the target pool is an enriched pool when the free energy of the target aptamer sequence group is lower than the free energy of the reference aptamer sequence group. The reference aptamer sequence group is a candidate aptamer sequence group including a plurality of candidate aptamer sequences or a virtual aptamer sequence group that is derived from the target aptamer sequence group and includes virtual aptamer sequences having the same base composition as the target aptamer sequence group.
摘要翻译：本发明提供一种预测装置，预测方法，程序和记录介质，可以容易地预测其中是否需要富集需要的适体序列。本发明的预测装置10包括输入单元11，计算单元12和预测单元13.输入单元11是通过目标池中包括所选择的适配体的目标适体序列组的序列信息和输入包括参考适体序列的参照适体序列组。计算单元12计算目标适体序列组的自由能和参考适体序列组的自由能。预测单元13比较这些序列组的自由能，并且当靶适体序列组的自由能低于参考适体序列组的自由能时，预测目标池是富集池。参照适体序列组是包含从目标适体序列组得到的多个候选适体序列或虚拟适体序列组的候选适体序列组，并且具有与靶适体序列组相同的基本组成的虚拟适配子序列。

7. 发明授权

US09382544B2 Aptamer that recognizes peptide 有权
标题翻译：识别肽的探针
公开(公告)号：US09382544B2
公开(公告)日：2016-07-05
申请号：US13320462
申请日：2010-05-14
申请人： Shotaro Tsuji , Jou Akitomi , Shintarou Katou , Iwao Waga , Takashi Ohtsu
发明人： Shotaro Tsuji , Jou Akitomi , Shintarou Katou , Iwao Waga , Takashi Ohtsu
IPC分类号： C12N15/115 , C07H21/02 , C12N15/113 , C12N15/10
CPC分类号： C12N15/115 , C07H21/02 , C12N15/1048 , C12N15/113 , C12N2310/113 , C12N2310/16 , C12N2320/50
摘要： An aptamer capable of binding to a histidine peptide is provided. A nucleic acid used as the aptamer capable of binding to a histidine peptide may be a nucleic acid containing the base sequence of SEQ ID NO: 17, SEQ ID NO: 18, or containing a base sequence obtained by substitution, deletion, addition, or insertion of one or more bases in SEQ ID NO: 17 or 18.
摘要翻译：提供了能够结合组氨酸肽的适体。用作能够结合组氨酸肽的适体的核酸可以是含有SEQ ID NO：17，SEQ ID NO：18的碱基序列或含有通过取代，缺失，添加或在SEQ ID NO：17或18中插入一个或多个碱基。

8. 发明申请

US20120129720A1 APTAMER THAT RECOGNIZES PEPTIDE 有权
标题翻译：认可肽的APTAMER
公开(公告)号：US20120129720A1
公开(公告)日：2012-05-24
申请号：US13320462
申请日：2010-05-14
申请人： Shotaro Tsuji , Jou Akitomi , Shintarou Katou , Iwao Waga , Takashi Ohtsu
发明人： Shotaro Tsuji , Jou Akitomi , Shintarou Katou , Iwao Waga , Takashi Ohtsu
IPC分类号： C40B30/04 , C12N15/63 , C12N15/115 , C07H21/02
CPC分类号： C12N15/115 , C07H21/02 , C12N15/1048 , C12N15/113 , C12N2310/113 , C12N2310/16 , C12N2320/50
摘要： An aptamer capable of binding to a histidine peptide is provided. A nucleic acid used as the aptamer capable of binding to a histidine peptide is any of the following nucleic acids (a) to (d): (a) a nucleic acid having a base sequence represented by SEQ ID NO: 17: GGUNnAYUmGGH (SEQ ID NO: 17), where in the nucleic acid (a), N represents A, G, C, U, or T, n of Nn represents the number of Ns and is an integer from 1 to 3, Y represents U, T, or C, m of Um represents the number of Us and is an integer from 1 to 3, and H represents U, T, C, or A; (b) a nucleic acid having a base sequence obtained by substitution, deletion, addition, or insertion of one or more bases in a base sequence of the nucleic acid (a) and being capable of binding to the histidine peptide; (c) a nucleic acid having a base sequence represented by SEQ ID NO: 18: GGCGCCUUCGUGGAAUGUC (SEQ ID NO: 18); and (d) a nucleic acid having a base sequence obtained by substitution, deletion, addition, or insertion of one or more bases in a base sequence of the nucleic acid (c) and being capable of binding to the histidine peptide.
摘要翻译：提供了能够结合组氨酸肽的适体。用作能够结合组氨酸肽的适体的核酸是以下任一核酸（a）至（d）：（a）具有由SEQ ID NO：17表示的碱基序列的核酸：GGUNnAYUmGGH（SEQ ID NO： ID号：17），其中在核酸（a）中，N表示A，G，C，U或T，N n表示N的数，为1〜3的整数，Y表示U，T 或C m表示Us的数，是1〜3的整数，H表示U，T，C或A; （b）具有通过在核酸（a）的碱基序列中取代，缺失，添加或插入一个或多个碱基并且能够结合组氨酸肽而获得的碱基序列的核酸; （c）具有由SEQ ID NO：18表示的碱基序列的核酸：GGCGCCUUCGUGGAAUGUC（SEQ ID NO：18）; 和（d）具有通过在核酸（c）的碱基序列中取代，缺失，添加或插入一个或多个碱基并且能够结合组氨酸肽而获得的碱基序列的核酸。

9. 发明授权

US09454642B2 Prediction device, prediction method, program, and recording medium 有权
标题翻译：预测装置，预测方法，程序和记录介质
公开(公告)号：US09454642B2
公开(公告)日：2016-09-27
申请号：US13807876
申请日：2011-07-02
申请人： Jou Akitomi , Shintarou Katou , Shotaro Tsuji , Iwao Waga
发明人： Jou Akitomi , Shintarou Katou , Shotaro Tsuji , Takashi Ohtsu , Iwao Waga
IPC分类号： G01N33/50 , G06F19/16 , C12N15/115 , G06F19/18
CPC分类号： G06F19/16 , C12N15/115 , C12N2310/16 , G06F19/18
摘要： The present invention provides a prediction device, a prediction method, a program, and a recording medium, with which whether or not desired aptamer sequences are enriched can be predicted easily. The prediction device of the present invention 10 includes an input unit 11, a calculation unit 12, and a prediction unit 13. The input unit 11 is a unit through which sequence information on a target aptamer sequence group including selected aptamers in a target pool and a reference aptamer sequence group including reference aptamer sequences are inputted. The calculation unit 12 calculates the free energy of the target aptamer sequence group and the free energy of the reference aptamer sequence group. The prediction unit 13 compares the free energy of these sequence groups, and predicts that the target pool is an enriched pool when the free energy of the target aptamer sequence group is lower than the free energy of the reference aptamer sequence group. The reference aptamer sequence group is a candidate aptamer sequence group including a plurality of candidate aptamer sequences or a virtual aptamer sequence group that is derived from the target aptamer sequence group and includes virtual aptamer sequences having the same base composition as the target aptamer sequence group.
摘要翻译：本发明提供一种预测装置，预测方法，程序和记录介质，可以容易地预测其中是否需要富集需要的适体序列。本发明的预测装置10包括输入单元11，计算单元12和预测单元13.输入单元11是通过目标池中包括所选择的适配体的目标适体序列组的序列信息和输入包括参考适体序列的参照适体序列组。计算单元12计算目标适体序列组的自由能和参考适体序列组的自由能。预测单元13比较这些序列组的自由能，并且当靶适体序列组的自由能低于参考适体序列组的自由能时，预测目标池是富集池。参照适体序列组是包含从目标适体序列组得到的多个候选适体序列或虚拟适体序列组的候选适体序列组，并且具有与靶适体序列组相同的基本组成的虚拟适配子序列。

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