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1. 发明申请

US20120042401A1 METHOD FOR INTRODUCING MUTANT GENE, GENE HAVING MUTATION INTRODUCED THEREIN, CASSETTE FOR INTRODUCING MUTATION, VECTOR FOR INTRODUCING MUTATION, AND KNOCK-IN NON-HUMAN MAMMALIAN ANIMAL 审中-公开
标题翻译：引入突变基因的方法，引入突变体的基因，引入突变的CASSETTE，引入突变的载体和非人类哺乳动物动物
公开(公告)号：US20120042401A1
公开(公告)日：2012-02-16
申请号：US13125775
申请日：2009-10-23
申请人： Shinichi Hirose , Masanobu Deshimaru , Kimi Araki
发明人： Shinichi Hirose , Masanobu Deshimaru , Kimi Araki
IPC分类号： C12N15/85 , C12P19/34
CPC分类号： A01K67/0275 , A01K2217/072 , A01K2227/105 , A01K2267/0306 , C07K14/705 , C12N15/8509 , C12N15/907 , C12N2800/107 , C12N2800/30
摘要： Disclosed is a method for introducing a mutation into a gene, which comprises the following steps: a homologous recombination step of carrying out the homologous recombination between a target gene into which the mutation is to be introduced and a target recombinant vector, thereby substituting an exon in the target gene into which the mutation is to be introduced by a target DNA sequence in the target recombinant vector; and a mutation introduction step of carrying out the specific recombination between the target DNA sequence in the resulting target recombinant gene and a mutation introduction cassette of a mutation introduction vector carrying a mutated DNA sequence containing a mutant exon by the intervening action of Cre recombinase to substitute the target DNA sequence by the mutated DNA in the mutation introduction cassette, thereby producing a mutation-introduced gene into which the mutant DNA sequence has been introduced. The method enables the production of a knock-in non-human mammalian animal, such as a knock-in mouse, which carries the mutation-introduced gene.
摘要翻译：公开了将突变引入基因的方法，其包括以下步骤：将引入突变的靶基因与靶重组载体进行同源重组的同源重组步骤，从而取代外显子在目标重组载体中由靶DNA序列引入突变的靶基因中; 以及突变引入步骤，通过Cre重组酶的干预作用来进行所得到的靶重组基因中的靶DNA序列与携带含有突变外显子的突变的DNA序列的突变引入载体的突变导入盒进行替代的特异性重组通过突变导入盒中的突变DNA的靶DNA序列，从而产生引入突变DNA序列的突变导入基因。该方法能够产生携带突变导入基因的敲入非人哺乳动物，例如敲入小鼠。

2. 发明申请

US20140289878A1 METHOD FOR PRODUCING MODEL ANIMAL, AND MODEL ANIMAL 有权
标题翻译：生产动物模型和模型动物的方法
公开(公告)号：US20140289878A1
公开(公告)日：2014-09-25
申请号：US14355432
申请日：2012-06-19
申请人： Masaki Ohmuraya , Kimi Araki
发明人： Masaki Ohmuraya , Kimi Araki
IPC分类号： A01K67/027 , C12N5/0735 , C12N15/85
CPC分类号： A01K67/0276 , A01K67/0271 , A01K2217/05 , A01K2227/105 , A01K2267/03 , C12N5/0606 , C12N15/8509 , C12N15/873
摘要： A method for producing a model animal which has a desired lifetime, and in which a predetermined biological reaction can be induced, and a model animal are provided. The present invention produces a first individual in which a gene of interest is heterozygously deficient using a first ES cell from a non-human mammalian animal. Meanwhile, a fragment containing a homologous gene that has homology to the gene of interest is made, a second ES cell constituted so that a predetermined region on X chromosome of the animal can be substituted is used, and the fragment is introduced into the second ES cell to generate a substituted ES cell in which the predetermined region has been substituted with the fragment. A second individual is produced using the substituted ES cell. The first individual and the second individual are mated with each other to produce a model animal.
摘要翻译：提供具有期望寿命并且可以诱导预定生物反应的模型动物的制备方法和模型动物。本发明使用来自非人哺乳动物的第一ES细胞产生其中感兴趣的基因是杂合缺陷的第一个体。同时，使用含有与感兴趣的基因具有同源性的同源基因的片段，构成第二ES细胞，使得动物的X染色体上的预定区域可以被取代，并将该片段引入第二ES 以产生其中预定区域已被该片段替代的取代的ES细胞。使用取代的ES细胞产生第二个个体。第一个人和第二个人相互交配，形成一个示范动物。

3. 发明申请

US20150052625A1 HUMANIZED MOUSE 审中-公开
标题翻译：人造鼠
公开(公告)号：US20150052625A1
公开(公告)日：2015-02-19
申请号：US14387704
申请日：2012-03-27
申请人： Ken-ichi Yamamura , Kimi Araki , Seiji Okada , Akihiko Shimono
发明人： Ken-ichi Yamamura , Kimi Araki , Seiji Okada , Akihiko Shimono
IPC分类号： A01K67/027 , C12N5/0735
CPC分类号： A01K67/0278 , A01K67/0271 , A01K2207/12 , A01K2207/20 , A01K2217/075 , A01K2227/105 , A01K2267/0331 , A01K2267/035 , C12N5/0606 , C12N2517/02
摘要： The present invention provides embryonic stem cells obtainable from an embryo of an immunodeficient mouse which is deficient in both Rag2 and Jak3 genes by culture in the presence of a GSK3 inhibitor and an MEK inhibitor, as well as a transgenic mouse, which is created with the use of these embryonic stem cells.
摘要翻译：本发明提供了通过在GSK3抑制剂和MEK抑制剂的存在下培养而Rag2和Jak3基因缺陷的免疫缺陷小鼠的胚胎获得的胚胎干细胞，以及转基因小鼠，其由使用这些胚胎干细胞。

4. 发明申请

US20090202988A1 Trap vectors and gene trapping using the same 审中-公开
标题翻译：陷阱载体和使用相同的基因捕获
公开(公告)号：US20090202988A1
公开(公告)日：2009-08-13
申请号：US11907497
申请日：2007-10-12
申请人： Ken-ichi Yamamura , Kimi Araki
发明人： Ken-ichi Yamamura , Kimi Araki
IPC分类号： C12Q1/68 , C12N15/00 , C12N5/06
CPC分类号： C12N15/1051 , C12N15/10
摘要： A trap vector containing a loxP sequence composed of inverted repeat sequence 1, a spacer sequence and inverted repeat sequence 2 in this order, the loxP sequence being a mutant loxP wherein a part of the inverted repeat sequence 1 or 2 is mutated.
摘要翻译：含有由反向重复序列1，间隔序列和反向重复序列2构成的loxP序列的陷阱载体，loxP序列是突变型loxP，其中反向重复序列1或2的一部分突变。

5. 发明授权

US08722408B2 Trap vectors and gene trapping method by using the same 有权
标题翻译：陷阱载体和基因捕获方法使用相同
公开(公告)号：US08722408B2
公开(公告)日：2014-05-13
申请号：US12987601
申请日：2011-01-10
申请人： Ken-ichi Yamamura , Kimi Araki
发明人： Ken-ichi Yamamura , Kimi Araki
IPC分类号： C12N15/85 , C12N15/63
CPC分类号： C12N15/1051 , C12N15/10
摘要： A trap vector containing a loxP sequence composed of inverted repeat sequence 1, a spacer sequence and inverted repeat sequence 2 in this order, the loxP sequence being a mutant loxP wherein a part of the inverted repeat sequence 1 or 2 is mutated.
摘要翻译：含有由反向重复序列1，间隔序列和反向重复序列2构成的loxP序列的陷阱载体，loxP序列是突变型loxP，其中反向重复序列1或2的一部分突变。

6. 发明申请

US20130211187A1 Method of Establishing Mouse Strain 有权
标题翻译：建立小鼠应变的方法
公开(公告)号：US20130211187A1
公开(公告)日：2013-08-15
申请号：US13818906
申请日：2011-08-30
申请人： Masatake Araki , Kimi Araki
发明人： Masatake Araki , Kimi Araki
IPC分类号： A01K67/027 , A61D19/04
CPC分类号： A01K67/0271 , A01K2227/105 , A61D19/04
摘要： A method of producing a chimera animal, characterized by transplanting, into a host animal, an aggregate of a first pluripotent stem cell that is of interest to strain establishment, a second pluripotent stem cell in which the ability to form germ cells is absent or decreased, and embryo.
摘要翻译：一种生产嵌合体动物的方法，其特征在于将宿主动物移植到菌株建立所关注的第一多能干细胞的集合体中，其中形成生殖细胞的能力不存在或减少的第二个多能干细胞，和胚胎。

7. 发明申请

US20110117642A1 TRAP VECTORS AND GENE TRAPPING METHOD BY USING THE SAME 有权
标题翻译：捕捉矢量和使用它的基因捕获方法
公开(公告)号：US20110117642A1
公开(公告)日：2011-05-19
申请号：US12987601
申请日：2011-01-10
申请人： Ken-ichi YAMAMURA , Kimi Araki
发明人： Ken-ichi YAMAMURA , Kimi Araki
IPC分类号： C12N15/63
CPC分类号： C12N15/1051 , C12N15/10
摘要： A trap vector containing a loxP sequence composed of inverted repeat sequence 1, a spacer sequence and inverted repeat sequence 2 in this order, the loxP sequence being a mutant loxP wherein a part of the inverted repeat sequence 1 or 2 is mutated.
摘要翻译：含有由反向重复序列1，间隔序列和反向重复序列2构成的loxP序列的陷阱载体，loxP序列是突变型loxP，其中反向重复序列1或2的一部分突变。

8. 发明授权

US10010059B2 Method for producing model animal, and model animal 有权
公开(公告)号：US10010059B2
公开(公告)日：2018-07-03
申请号：US14355432
申请日：2012-06-19
申请人： Masaki Ohmuraya , Kimi Araki
发明人： Masaki Ohmuraya , Kimi Araki
IPC分类号： A01K67/00 , A01K67/033 , C12N15/00 , A01K67/027 , C12N15/873 , C12N15/85 , C12N5/0735
CPC分类号： A01K67/0276 , A01K67/0271 , A01K2217/05 , A01K2227/105 , A01K2267/03 , C12N5/0606 , C12N15/8509 , C12N15/873
摘要： A method for producing a model animal which has a desired lifetime, and in which a predetermined biological reaction can be induced, and a model animal are provided. The present invention produces a first individual in which a gene of interest is heterozygously deficient using a first ES cell from a non-human mammalian animal. Meanwhile, a fragment containing a homologous gene that has homology to the gene of interest is made, a second ES cell constituted so that a predetermined region on X chromosome of the animal can be substituted is used, and the fragment is introduced into the second ES cell to generate a substituted ES cell in which the predetermined region has been substituted with the fragment. A second individual is produced using the substituted ES cell. The first individual and the second individual are mated with each other to produce a model animal.

9. 发明授权

US09167805B2 Method of establishing mouse strain 有权
标题翻译：建立小鼠应变的方法
公开(公告)号：US09167805B2
公开(公告)日：2015-10-27
申请号：US13818906
申请日：2011-08-30
申请人： Masatake Araki , Kimi Araki
发明人： Masatake Araki , Kimi Araki
IPC分类号： A01K67/027 , A61D19/04
CPC分类号： A01K67/0271 , A01K2227/105 , A61D19/04
摘要： A method of producing a chimera animal, characterized by transplanting, into a host animal, an aggregate of a first pluripotent stem cell that is of interest to strain establishment, a second pluripotent stem cell in which the ability to form germ cells is absent or decreased, and embryo.
摘要翻译：一种生产嵌合体动物的方法，其特征在于将宿主动物移植到菌株建立所关注的第一多能干细胞的集合体中，其中形成生殖细胞的能力不存在或减少的第二个多能干细胞，和胚胎。

10. 发明授权

US07312075B1 Trap vectors and gene trapping by using the same 失效
标题翻译：陷阱载体和基因捕获使用相同
公开(公告)号：US07312075B1
公开(公告)日：2007-12-25
申请号：US10030658
申请日：2000-05-02
申请人： Ken-ichi Yamamura , Kimi Araki
发明人： Ken-ichi Yamamura , Kimi Araki
IPC分类号： C12N15/63
CPC分类号： C12N15/1051 , C12N15/10
摘要： A trap vector containing a loxP sequence composed of inverted repeat sequence 1, a spacer sequence and inverted repeat sequence 2 in this order, the loxP sequence being a mutant loxP wherein a part of the inverted repeat sequence 1 or 2 is mutated.
摘要翻译：含有由反向重复序列1，间隔序列和反向重复序列2构成的loxP序列的陷阱载体，loxP序列是突变型loxP，其中反向重复序列1或2的一部分突变。

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