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1. 发明申请

US20110136234A1 CELL CULTURE SUBSTRATE AND ITS PRODUCTION METHOD 有权
标题翻译：细胞培养基质及其生产方法
公开(公告)号：US20110136234A1
公开(公告)日：2011-06-09
申请号：US13020193
申请日：2011-02-03
申请人： Shin OHYA , Takashi Inoue , Takatoki Yamamoto , Teruo Fujii , Yasuyuki Sakai , Masaki Nishikawa , Hitomi Sakai , Hirosuke Naruto
发明人： Shin OHYA , Takashi Inoue , Takatoki Yamamoto , Teruo Fujii , Yasuyuki Sakai , Masaki Nishikawa , Hitomi Sakai , Hirosuke Naruto
IPC分类号： C12N5/07 , C12N5/0735
CPC分类号： C12N5/0068 , C08G2261/3424 , C08L65/04 , C12N2533/30
摘要： A cell culture substrate which is durable and which can be readily produced in commercial scale at a low cost, and its production method are provided. The cell culture substrate comprises a substrate and a layer formed by surface modification, which comprises a polymer containing amino group produced by reacting a polymer represented by the following formula (II): (wherein n is 0 or a positive integer, and m is a positive integer, the n and m representing degree of polymerization) formed by chemical vapor deposition of formyl[2.2]paracyclophane represented by the following formula (I): (wherein k is 0 or 1) with a polymer having at least one amino group (—NH2) capable of forming Schiff base in its monomer. The production method of the cell culture substrate comprises the step of synthesizing such polymer on the substrate.
摘要翻译：提供耐久性且能够以低成本容易地以商业规模生产的细胞培养基材及其制造方法。细胞培养基材包括基材和通过表面改性形成的层，其包含通过使由下式（II）表示的聚合物反应而生成的含氨基的聚合物：（其中n为0或正整数，m为正整数，代表聚合度的n和m）通过化学气相沉积由下式（I）表示的甲酰基[2.2]对环烷烃形成：（其中k为0或1）与具有至少一个氨基的聚合物 -NH2）能够在其单体中形成席夫碱。细胞培养基质的制造方法包括在基材上合成这样的聚合物的工序。

2. 发明申请

US20090042295A1 Cell culture substrate and its production method 审中-公开
标题翻译：细胞培养基质及其制备方法
公开(公告)号：US20090042295A1
公开(公告)日：2009-02-12
申请号：US11889067
申请日：2007-08-08
申请人： Shin Ohya , Takashi Inoue , Takatoki Yamamoto , Teruo Fujii , Yasuyuki Sakai , Masaki Nishikawa , Hitomi Sakai , Hirosuke Naruto
发明人： Shin Ohya , Takashi Inoue , Takatoki Yamamoto , Teruo Fujii , Yasuyuki Sakai , Masaki Nishikawa , Hitomi Sakai , Hirosuke Naruto
IPC分类号： C12N5/06
CPC分类号： C12N5/0068 , C08G2261/3424 , C08L65/04 , C12N2533/30
摘要： A cell culture substrate which is durable and which can be readily produced in commercial scale at a low cost, and its production method are provided. The cell culture substrate comprises a substrate and a layer formed by surface modification, which comprises a polymer containing amino group produced by reacting a polymer represented by the following formula (II): (wherein n is 0 or a positive integer, and m is a positive integer, the n and m representing degree of polymerization) formed by chemical vapor deposition of formyl[2.2]paracyclophane represented by the following formula (I): (wherein k is 0 or 1) with a polymer having at least one amino group (—NH2) capable of forming Schiff base in its monomer. The production method of the cell culture substrate comprises the step of synthesizing such polymer on the substrate.
摘要翻译：提供耐久性且能够以低成本容易地以商业规模生产的细胞培养基材及其制造方法。细胞培养基材包括基材和通过表面改性形成的层，其包含通过使由下式（II）表示的聚合物反应而生成的含氨基的聚合物：（其中n为0或正整数，m为正整数，代表聚合度的n和m）通过化学气相沉积由下式（I）表示的甲酰基[2.2]对环烷烃形成：（其中k为0或1）与具有至少一个氨基的聚合物 -NH2）能够在其单体中形成席夫碱。细胞培养基质的制造方法包括在基材上合成这样的聚合物的工序。

3. 发明授权

US08383409B2 Cell culture substrate and its production method 有权
标题翻译：细胞培养基质及其制备方法
公开(公告)号：US08383409B2
公开(公告)日：2013-02-26
申请号：US13020193
申请日：2011-02-03
申请人： Shin Ohya , Takashi Inoue , Takatoki Yamamoto , Teruo Fujii , Yasuyuki Sakai , Masaki Nishikawa , Hitomi Sakai , Hirosuke Naruto
发明人： Shin Ohya , Takashi Inoue , Takatoki Yamamoto , Teruo Fujii , Yasuyuki Sakai , Masaki Nishikawa , Hitomi Sakai , Hirosuke Naruto
IPC分类号： C12N5/02
CPC分类号： C12N5/0068 , C08G2261/3424 , C08L65/04 , C12N2533/30
摘要： The invention is directed to methods for the propagation or cultivation of cells including preparing a cell culture substrate, wherein the cell culture substrate includes a substrate and a layer formed by surface modification. The layer includes a polymer containing an amino group. The polymer is produced by reacting a polymer represented by formula (II): with a polymer having at least one amino group, —NH2, capable of forming a Schiff base in a monomer of formula (II), thereby forming a polymer layer constituting the layer formed by surface modification. “n” in Formula (II) is 0 or a positive integer, and m is a positive integer. n and m represent the degree of polymerization. Formula (II) is formed by chemical vapor deposition of formyl[2.2]paracyclophane. The methods further include providing cells in a medium; inoculating the cells onto the cell culture substrate; and culturing the cells, wherein the cells adhere to the cell culture substrate.
摘要翻译：本发明涉及细胞繁殖或培养方法，包括制备细胞培养基质，其中细胞培养基质包括基质和通过表面改性形成的层。该层包括含有氨基的聚合物。该聚合物通过使由式（II）表示的聚合物与具有至少一个氨基的聚合物-NH 2反应生成，该聚合物能够在式（II）的单体中形成席夫碱，从而形成构成通过表面改性形成的层。式（II）中的n为0或正整数，m为正整数。 n和m表示聚合度。式（II）由甲酰基[2.2]对环磷酰胺的化学气相沉积形成。所述方法还包括在培养基中提供细胞; 将细胞接种到细胞培养基底上; 并培养细胞，其中细胞粘附于细胞培养基质。

4. 发明申请

US20070212773A1 Electrical signal measuring device for cells in culture and electrical signal measuring method that uses same device 审中-公开
标题翻译：用于培养细胞的电信号测量装置和使用相同装置的电信号测量方法
公开(公告)号：US20070212773A1
公开(公告)日：2007-09-13
申请号：US11707588
申请日：2007-02-15
申请人： Teruo Fujii , Yasuyuki Sakai , Serge Ostrovidov
发明人： Teruo Fujii , Yasuyuki Sakai , Serge Ostrovidov
IPC分类号： C12M1/34
CPC分类号： C12M41/36 , C12M29/04 , C12M29/10 , C12M41/46 , G01N33/4833
摘要： An electrical signal measuring device that can measure electrical characteristics of cells in culture in real time and with high accuracy while maintaining them in a favorable state and an electrical signal measuring method that uses the device are disclosed. The electrical signal measuring device 1 for cells in culture includes a support body 2, a compartment in the support body, a semipermeable membrane 3, and electrodes, in which the compartment is divided by the semipermeable membrane 3 into an upper compartment 4 and a lower compartment 5; an upper electrode 61 is provided in the upper compartment 4, and a lower electrode 62 that faces the upper electrode 61 and whose facing surface 621 is in contact with the semipermeable membrane 3 is provided in the lower compartment 5; and an upper perfusion channel and a lower perfusion channel are provided in the support body 2 to separately perfuse fluid in the upper compartment 4 and the lower compartment 5 is disclosed.
摘要翻译：公开了一种电信号测量装置，其能够实时且准确地测量培养中的细胞的电特性，同时保持它们处于良好状态，并且使用该装置的电信号测量方法。用于培养细胞的电信号测量装置1包括支撑体2，支撑体中的隔室，半透膜3和电极，其中隔室被半透膜3分成上隔室4和下隔室隔间5; 在上部隔间4设置有上部电极61，并且在下部隔室5中设置面向上部电极61并且其面对面621与半透膜3接触的下部电极62; 并且在支撑体2中设置有上部灌注通道和下部灌注通道，以分开地灌注上部隔室4中的流体，并且公开了下部隔室5。

5. 发明授权

US08906644B2 Method for culture of hepatocytes 有权
标题翻译：肝细胞培养方法
公开(公告)号：US08906644B2
公开(公告)日：2014-12-09
申请号：US13392226
申请日：2010-07-28
申请人： Hitoshi Matsui , Yasuyuki Sakai , Teruo Fujii , Shoji Takeuchi , Yukiko Tsuda
发明人： Hitoshi Matsui , Yasuyuki Sakai , Teruo Fujii , Shoji Takeuchi , Yukiko Tsuda
IPC分类号： G01N21/64 , C12M1/12 , C12M1/34 , C12M1/00 , C12N5/071 , C12M1/04 , G01N33/50
CPC分类号： C12M23/20 , C12M23/24 , C12M25/14 , C12M41/46 , C12N5/0671 , C12N2503/00 , C12N2533/30 , C12N2533/54 , G01N33/5044
摘要： A method for culturing hepatocytes, wherein hepatocytes embedded in an extracellular matrix is placed on a gas-permeable membrane and the hepatocytes are cultured while being supplied with oxygen from the gas-permeable membrane side. By this, the polarity in the hepatocytes can be induced and a bile canaliculus can be formed in a short period of time. Further, the formed polarity can be maintained for a longer period.
摘要翻译：一种培养肝细胞的方法，其中将包埋在细胞外基质中的肝细胞置于透气膜上，并且在从透气膜侧供给氧的同时培养肝细胞。由此，可以诱导肝细胞中的极性，并且可以在短时间内形成胆小管。此外，所形成的极性可以保持更长的时间。

6. 发明申请

US20120183989A1 METHOD FOR CULTURE OF HEPATOCYTES 有权
标题翻译：肝细胞培养方法
公开(公告)号：US20120183989A1
公开(公告)日：2012-07-19
申请号：US13392226
申请日：2010-07-28
申请人： Hitoshi Matsui , Yasuyuki Sakai , Teruo Fujii , Shoji Takeuchi , Yukiko Tsuda
发明人： Hitoshi Matsui , Yasuyuki Sakai , Teruo Fujii , Shoji Takeuchi , Yukiko Tsuda
IPC分类号： G01N21/64 , C12Q1/02 , C12M1/34 , C12N5/071
CPC分类号： C12M23/20 , C12M23/24 , C12M25/14 , C12M41/46 , C12N5/0671 , C12N2503/00 , C12N2533/30 , C12N2533/54 , G01N33/5044
摘要： A method for culturing hepatocytes, wherein hepatocytes embedded in an extracellular matrix is placed on a gas-permeable membrane and the hepatocytes are cultured while being supplied with oxygen from the gas-permeable membrane side. By this, the polarity in the hepatocytes can be induced and a bile canaliculus can be formed in a short period of time. Further, the formed polarity can be maintained for a longer period.
摘要翻译：一种培养肝细胞的方法，其中将包埋在细胞外基质中的肝细胞置于透气膜上，并且在从透气膜侧供给氧的同时培养肝细胞。由此，可以诱导肝细胞中的极性，并且可以在短时间内形成胆小管。此外，所形成的极性可以保持更长的时间。

7. 发明申请

US20080145925A1 Cell Culture Chamber 审中-公开
标题翻译：细胞培养室
公开(公告)号：US20080145925A1
公开(公告)日：2008-06-19
申请号：US11885889
申请日：2005-12-08
申请人： Yasuyuki Sakai , Teruo Fujii , Serge Ostrovidov , Hiroaki Inui , Jinji Mizuno
发明人： Yasuyuki Sakai , Teruo Fujii , Serge Ostrovidov , Hiroaki Inui , Jinji Mizuno
IPC分类号： C12M1/12
CPC分类号： C12M35/08 , C12M23/34 , C12M29/10
摘要： A cell culture chamber includes: a top compartment and a bottom compartment that are separated by a semi-permeable membrane inside a supporting body; a culture solution supply flow path that supplies culture solution to an interior of the bottom compartment; a culture solution discharge flow path that discharges culture solution from the interior of the bottom compartment; a circulation flow path that is used to circulate fluid within the interior of the top compartment; and a cell culturing portion is formed inside the top compartment by a sieve structure that is formed by aperture portions through which a fluid is able to pass but through which cultured cells cannot pass, and by wall portions.
摘要翻译：细胞培养室包括：顶部隔室和底部隔室，其由支撑体内的半透膜隔开; 将培养液供给到所述底部室的内部的培养液供给流路; 从底部室的内部排出培养液的培养液排出流路; 用于使流体在顶部隔间的内部循环的循环流动路径; 并且细胞培养部分通过筛孔结构形成在顶部隔室内，该筛结构由流体能够通过的通孔，通过培养的细胞不能通过的孔部分和壁部形成。

8. 发明授权

US08659920B2 Switching device provided with a flowing restriction element 有权
标题翻译：具有流动限制元件的开关装置
公开(公告)号：US08659920B2
公开(公告)日：2014-02-25
申请号：US13314499
申请日：2011-12-08
申请人： Nobuhisa Yamaguchi , Yasuyuki Sakai
发明人： Nobuhisa Yamaguchi , Yasuyuki Sakai
IPC分类号： H02H7/122
CPC分类号： H02M1/34 , H01L2224/48091 , H01L2224/73265 , H01L2924/1301 , H01L2924/1305 , H01L2924/13055 , H01L2924/30107 , H02M2001/348 , H01L2924/00014 , H01L2924/00
摘要： A switching device includes a flowing restriction element, a conductor and a snubber resistor. The flowing restriction element has an opening and closing function to open and close a flowing path of an electric current. The conductor is connected to the flowing restriction element. The snubber resistor is connected to the flowing restriction element and constitutes a snubber circuit. The snubber resistor is disposed along the conductor.
摘要翻译：开关装置包括流动限制元件，导体和缓冲电阻器。流动限制元件具有打开和关闭电流流动通道的打开和关闭功能。导体连接到流动的限制元件。缓冲电阻器连接到流动限制元件并构成缓冲电路。缓冲电阻器沿着导体设置。

9. 发明授权

US5140141A Bar-code reader with reading zone indicator 失效
标题翻译：具有阅读区指示器的条码阅读器
公开(公告)号：US5140141A
公开(公告)日：1992-08-18
申请号：US581217
申请日：1990-09-12
申请人： Masahiro Inagaki , Osamu Asai , Yasuyuki Sakai
发明人： Masahiro Inagaki , Osamu Asai , Yasuyuki Sakai
IPC分类号： G06K7/10
CPC分类号： G06K7/10
摘要： A bar-code reader for reading bar codes on articles or commodities in supermarkets or retail stores has a scanning unit mounted on a vertically movable support post assembly and including a scanning system which emits a scanning light beam and detects light reflected from a bar code. The bar-code reader includes various indicating members, such as an indicator rod, an indicator ring, an arrow mark, a pattern mark, and a colored sheet, for indicating to the operator a direction, a position, and an area or zone in which to pass an article with a bar code in order for the bar code to be successully read by the scanning system.
摘要翻译：用于读取超市或零售商店中的物品或商品上的条形码的条形码读取器具有安装在可垂直移动的支撑柱组件上的扫描单元，并且包括发射扫描光束并检测从条形码反射的光的扫描系统。条形码阅读器包括诸如指示杆，指示器环，箭头标记，图案标记和着色纸的各种指示构件，用于向操作者指示方向，位置和区域或区域通过条形码传递文章，以便条形码被扫描系统顺利读取。

10. 发明授权

US4005576A Internal combustion engine exhaust manifold with cylindrical built-in catalyst container 失效
标题翻译：具有圆柱形内置催化剂容器的内燃机排气歧管
公开(公告)号：US4005576A
公开(公告)日：1977-02-01
申请号：US569558
申请日：1975-04-18
申请人： Hidetaka Nohira , Hironori Bessho , Yasuyuki Sakai
发明人： Hidetaka Nohira , Hironori Bessho , Yasuyuki Sakai
IPC分类号： F01N3/28 , F01N3/34 , F01N13/10 , F01N3/15
CPC分类号： F01N13/10 , F01N3/2846 , F01N3/34 , F01N2270/04 , F01N2330/08 , F01N2430/06 , Y02T10/20
摘要： An internal combustion engine exhaust manifold with cylindrical built-in catalyst container which comprises cylindrical inner and outer cylinders and annular lids fitting at opposite ends thereof and is filled with a catalyst suitable for cleaning the engine exhaust.An exhaust gas cleaning device using the exhaust manifold comprises an oxygen concentration sensor located in the exhaust gas flow and an electronic fuel injector for performing feedback control in accordance with a signal from the oxygen concentration sensor.
摘要翻译：具有圆柱形内置催化剂容器的内燃机排气歧管，其包括圆柱形内外圆筒和安装在其相对端的环形盖，并且填充有适于清洁发动机排气的催化剂。

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