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1. 发明授权

US07951997B2 Reversible male sterility in transgenic plants by expression of cytokinin oxidase 有权
标题翻译：通过表达细胞分裂素氧化酶，在转基因植物中可逆雄性不育
公开(公告)号：US07951997B2
公开(公告)日：2011-05-31
申请号：US11735793
申请日：2007-04-16
申请人： Shihshieh Huang , Lyle Dean Crossland , Roy O. Morris , Nordine Cheikh
发明人： Shihshieh Huang , Lyle Dean Crossland , Roy O. Morris , Nordine Cheikh
IPC分类号： C12N15/82 , C12N15/53 , A01H1/02 , A01H5/00 , A01H5/10
CPC分类号： C12N9/0032 , C12N15/8289
摘要： Methods for the production of reversibly male-sterile plants by introduction of a polynucleotide encoding a cytokinin oxidase are disclosed along with nucleic acid constructs and transformed cells useful in the production of such plants. Also disclosed is the use of plants containing recombinant nucleic acid sequences in preventing pollination of plants with pollen containing one or more transgenes and in the introduction of economically important traits into elite varieties of plants.
摘要翻译：公开了通过引入编码细胞分裂素氧化酶的多核苷酸生产可逆雄性不育植物的方法以及用于生产这种植物的核酸构建体和转化细胞。还公开了含有重组核酸序列的植物在防止含有一种或多种转基因花粉的植物授粉以及将经济上重要的性状引入植物精英品种中的用途。

2. 发明申请

US20100242132A1 Reversible Male Sterility in Transgenic Plants by Expression of Cytokinin Oxidase 有权
标题翻译：通过细胞分裂素氧化酶的表达，转基因植物中的可逆雄性不育
公开(公告)号：US20100242132A1
公开(公告)日：2010-09-23
申请号：US11735793
申请日：2007-04-16
申请人： Shihshieh Huang , Lyle Dean Crossland , Nordine Cheikh , Roy O. Morris
发明人： Shihshieh Huang , Lyle Dean Crossland , Nordine Cheikh , Roy O. Morris
IPC分类号： A01H1/00 , A01H5/00
CPC分类号： C12N9/0032 , C12N15/8289
摘要： Methods for the production of reversibly male-sterile plants by introduction of a polynucleotide encoding a cytokinin oxidase are disclosed along with nucleic acid constructs and transformed cells useful in the production of such plants. Also disclosed is the use of plants containing recombinant nucleic acid sequences in preventing pollination of plants with pollen containing one or more transgenes and in the introduction of economically important traits into elite varieties of plants.
摘要翻译：公开了通过引入编码细胞分裂素氧化酶的多核苷酸生产可逆雄性不育植物的方法以及用于生产这种植物的核酸构建体和转化细胞。还公开了含有重组核酸序列的植物在防止含有一种或多种转基因花粉的植物授粉以及将经济上重要的性状引入植物精英品种中的用途。

3. 发明授权

US07230168B2 Reversible male sterility in transgenic plants by expression of cytokinin oxidase 有权
标题翻译：通过表达细胞分裂素氧化酶，在转基因植物中可逆雄性不育
公开(公告)号：US07230168B2
公开(公告)日：2007-06-12
申请号：US10326184
申请日：2002-12-20
申请人： Shihshieh Huang , Lyle Dean Crossland , Nordine Cheikh , Roy O. Morris
发明人： Shihshieh Huang , Lyle Dean Crossland , Nordine Cheikh , Roy O. Morris
IPC分类号： A01H5/00 , A01H5/10 , A01H1/02 , C12N5/04 , C12N15/82 , C12N15/29
CPC分类号： C12N9/0032 , C12N15/8289
摘要： Methods for the production of reversibly male-sterile plants by introduction of a polynucleotide encoding a cytokinin oxidase are disclosed along with nucleic acid constructs and transformed cells useful in the production of such plants. Also disclosed are the use of plants containing recombinant nucleic acid sequences in preventing pollination of plants with pollen containing one or more transgenes and in the introduction of economically important traits into elite varieties of plants.
摘要翻译：公开了通过引入编码细胞分裂素氧化酶的多核苷酸生产可逆雄性不育植物的方法以及用于生产这种植物的核酸构建体和转化细胞。还公开了含有重组核酸序列的植物在防止含有一种或多种转基因花粉的植物授粉以及将经济上重要的性状引入植物精英品种中的用途。

4. 发明授权

US06362394B1 Juvenile hormone or one of its agonists as a chemical ligand to control gene expression in plants by receptor mediated transactivation 失效
标题翻译：少年激素或其激动剂之一作为通过受体介导的反式激活控制植物中的基因表达的化学配体
公开(公告)号：US06362394B1
公开(公告)日：2002-03-26
申请号：US09376107
申请日：1999-08-17
申请人： Lyle Dean Crossland , Stephen Arthur Goff
发明人： Lyle Dean Crossland , Stephen Arthur Goff
IPC分类号： C12N1582
CPC分类号： C12N15/8238 , C07K14/70567 , C07K14/721 , C07K2319/00 , C12N15/8287
摘要： The present invention is drawn to a method of controlling gene expression in plants. Specifically, the method comprises transforming a plant with a USP receptor expression cassette which encodes a USP receptor and at least one target expression cassette which encodes a target polypeptide. Contacting said transformed plant with juvenile hormone or one of its agonists activates expression of the target polypeptide in the presence of said USP receptor polypeptide. Optionally, additional “secondary” receptor expression cassettes may be used, wherein the secondary receptor expression cassette encodes a receptor polypeptide distinct from USP. The method is useful for controlling various traits of agronomic importance, such as plant fertility. Also disclosed is a method of identifying previously unknown ligands for USP. Substances to be tested are identified by placing them in contact with plant cells transformed with a USP receptor expression cassette and a target expression cassette. The target expression cassette encodes a reporter polypeptide whose expression can be determined quantitatively or qualitatively, whereby the test substance is identified as a ligand for USP.
摘要翻译：本发明涉及控制植物中基因表达的方法。具体地，该方法包括用编码USP受体的USP受体表达盒和至少一个编码靶多肽的目标表达盒转化植物。将所述转化的植物与幼体激素或其一种激动剂接触在所述USP受体多肽的存在下激活靶多肽的表达。任选地，可以使用另外的“次级”受体表达盒，其中二级受体表达盒编码与USP不同的受体多肽。该方法可用于控制农艺学重要性的各种性状，如植物生育力。还公开了鉴定USP以前未知的配体的方法。通过将它们与用USP受体表达盒和目标表达盒转化的植物细胞接触来鉴定待测物质。靶表达盒编码报告多肽，其表达可以定量或定性地测定，由此将检测物质鉴定为USP的配体。

5. 发明授权

US06815577B1 Method of hybrid seed production using conditional female sterility 失效
标题翻译：使用条件雌性不育的杂交种子生产方法
公开(公告)号：US06815577B1
公开(公告)日：2004-11-09
申请号：US09032086
申请日：1998-02-27
申请人： Stacy Marie Harper , Lyle Dean Crossland , Erica Pascal
发明人： Stacy Marie Harper , Lyle Dean Crossland , Erica Pascal
IPC分类号： A01H102
CPC分类号： C12N15/8289 , C07K14/415 , C12N15/8222 , C12N15/8233 , C12N15/8261 , C12N15/829 , Y02A40/146
摘要： The present invention provides a method for hybrid seed production comprising producing a conditional female sterile plant comprising a female-preferential promoter operably linked to a coding sequence which encodes an enzyme which catalyzes the conversion of a protoxin to a toxin, interplanting the conditional female sterile plant with a male sterile plant, applying the protoxin to the conditional female sterile plant, and producing hybrid seed. Viable seed formation is prevented on the conditional female sterile plant as a result of the conversion of the protoxin to the toxin in the female reproductive structures, and pollen production is prevented on the male sterile plant, thus allowing interplanting of the two parents of the hybrid cross in order to provide more efficient pollen transfer. Also provided are expression cassettes useful in the invention, plants transformed with the expression cassette, and novel female-preferential promoters.
摘要翻译：本发明提供了一种用于杂种种子生产的方法，包括产生条件雌性不育植物，其包含与编码序列可操作连接的雌性优先启动子，所述编码序列编码催化原毒素向毒素转化的酶，将有条件的雌性不育植物与雄性不育植物，将前毒素施用于有条件的雌性不育植物，并产生杂种种子。由于原毒素转化为雌性生殖结构中的毒素，因此在有条件的雌性不育植物上防止了可行的种子形成，并且在雄性不育植物上防止了花粉产生，从而允许杂种的两个亲本的插入交叉以提供更有效的花粉转移。还提供了可用于本发明的表达盒，用表达盒转化的植物和新颖的雌性优先启动子。

6. 发明授权

US06392123B1 Female-preferential promoters isolated from maize and wheat 有权
标题翻译：从玉米和小麦分离的雌性优先启动子
公开(公告)号：US06392123B1
公开(公告)日：2002-05-21
申请号：US09575602
申请日：2000-05-22
申请人： Stacy Marie Harper , Lyle Dean Crossland , Erica Pascal
发明人： Stacy Marie Harper , Lyle Dean Crossland , Erica Pascal
IPC分类号： C12N1529
CPC分类号： C12N15/8289 , C07K14/415 , C12N15/8222 , C12N15/8233 , C12N15/8261 , C12N15/829 , Y02A40/146
摘要： The present invention provides a method for hybrid seed production comprising producing a conditional female sterile plant comprising a female-preferential promoter operably linked to a coding sequence which encodes an enzyme which catalyzes the conversion of a protoxin to a toxin, interplanting the conditional female sterile plant with a male sterile plant, applying the protoxin to the conditional female sterile plant, and producing hybrid seed. Viable seed formation is prevented on the conditional female sterile plant as a result of the conversion of the protoxin to the toxin in the female reproductive structures, and pollen production is prevented on the male sterile plant, thus allowing interplanting of the two parents of the hybrid cross in order to provide more efficient pollen transfer. Also provided are expression cassettes useful in the invention, plants transformed with the expression cassette, and novel female-preferential promoters.
摘要翻译：本发明提供了一种用于杂种种子生产的方法，包括产生条件雌性不育植物，其包含与编码序列可操作连接的雌性优先启动子，所述编码序列编码催化原毒素向毒素转化的酶，将有条件的雌性不育植物与雄性不育植物，将前毒素施用于有条件的雌性不育植物，并产生杂种种子。由于原毒素转化为雌性生殖结构中的毒素，因此在有条件的雌性不育植物上防止了可行的种子形成，并且在雄性不育植物上防止了花粉产生，从而允许杂种的两个亲本的插入交叉以提供更有效的花粉转移。还提供了可用于本发明的表达盒，用表达盒转化的植物和新颖的雌性优先启动子。

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