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1. 发明授权

US08642306B2 Flow cytometer having cell-sorting function and method of separating living cells 失效
标题翻译：具有细胞分选功能的流式细胞仪和分离活细胞的方法
公开(公告)号：US08642306B2
公开(公告)日：2014-02-04
申请号：US12663373
申请日：2008-06-11
申请人： Shigeyuki Nakada , Noriaki Kimura , Akihide Ito , Kenji Yasuda
发明人： Shigeyuki Nakada , Noriaki Kimura , Akihide Ito , Kenji Yasuda
IPC分类号： C12N13/00
CPC分类号： G01N15/147 , C12M47/04 , G01N2015/149
摘要： A liquid sample flow containing living cells is irradiated with measurement laser light and the photo data of at least either scattering light or fluorescence that is generated by each of the living cells in the liquid sample flow due to the irradiation with the measurement laser light is acquired. Based on the photo data thus acquired, it is determined whether each of the cells assignable to the respective photo data is an unnecessary living cell or a target living cell. Based on the determination results, a pulse voltage is then applied exclusively to the living cells having been determined as unnecessary living cells so that the unnecessary living cells are damaged and killed.
摘要翻译：用测量激光照射含有活细胞的液体样品流，并且获取由于测量激光的照射而在液体样品流中的每个活细胞产生的至少一种散射光或荧光的照片数据。基于这样获得的照片数据，确定分配给各个照片数据的每个细胞是否是不必要的活细胞或目标活细胞。基于测定结果，脉冲电压被专门用于被确定为不必要的活细胞的活细胞，使得不必要的活细胞被损坏和死亡。

2. 发明申请

US20100178682A1 FLOW CYTOMETER HAVING CELL-SORTING FUNCTION AND METHOD OF SEPARATING LIVING CELLS 失效
标题翻译：具有细胞分离功能的流式细胞仪和分离生物细胞的方法
公开(公告)号：US20100178682A1
公开(公告)日：2010-07-15
申请号：US12663373
申请日：2008-06-11
申请人： Shigeyuki Nakada , Noriaki Kimura , Akihide Ito , Kenji Yasuda
发明人： Shigeyuki Nakada , Noriaki Kimura , Akihide Ito , Kenji Yasuda
IPC分类号： C12N13/00 , C12M1/36
CPC分类号： G01N15/147 , C12M47/04 , G01N2015/149
摘要： A liquid sample flow containing living cells is irradiated with measurement laser light and the photo data of at least either scattering light or fluorescence that is generated by each of the living cells in the liquid sample flow due to the irradiation with the measurement laser light is acquired. Based on the photo data thus acquired, it is determined whether each of the cells assignable to the respective photo data is an unnecessary living cell or a target living cell. Based on the determination results, a pulse voltage is then applied exclusively to the living cells having been determined as unnecessary living cells so that the unnecessary living cells are damaged and killed.
摘要翻译：用测量激光照射含有活细胞的液体样品流，并且获取由于测量激光的照射而在液体样品流中的每个活细胞产生的至少一种散射光或荧光的照片数据。基于这样获得的照片数据，确定分配给各个照片数据的每个细胞是否是不必要的活细胞或目标活细胞。基于测定结果，脉冲电压被专门用于被确定为不必要的活细胞的活细胞，使得不必要的活细胞被损坏和死亡。

3. 发明授权

US08614099B2 FRET detection method and device 有权
标题翻译： FRET检测方法和装置
公开(公告)号：US08614099B2
公开(公告)日：2013-12-24
申请号：US12675323
申请日：2007-08-30
申请人： Shigeyuki Nakada , Noriaki Kimura
发明人： Shigeyuki Nakada , Noriaki Kimura
IPC分类号： G01N21/76 , G01N21/64
CPC分类号： G01N21/6428 , G01N21/6408 , G01N2021/6441
摘要： When FRET efficiency is measured quantitatively by removing uncertain elements of fluorescence detection information, calibration information prestored in a storage means while including at least the leak rate of donor fluorescence component emitted from a donor molecule, the leak rate of acceptor fluorescence component emitted from an acceptor molecule, and the non-FRET fluorescence lifetime of the donor fluorescence component when FRET is not generated out of the fluorescence of a measurement object sample is acquired. The FRET fluorescence lifetime of the donor fluorescence component is then determined using the intensity information and phase information of fluorescence of the measurement object sample, the leak rate of donor fluorescence component and the leak rate of acceptor fluorescence component, thus determining the FRET fluorescence efficiency.
摘要翻译：当通过消除荧光检测信息的不确定因素，预先存储在存储装置中的校准信息同时至少包括从供体分子发射的供体荧光成分的泄漏率，从受体发射的受体荧光成分的泄漏率，定量测量FRET效率并且获得当测量对象样品的荧光不产生FRET时供体荧光成分的非FRET荧光寿命。然后使用测量对象样品的荧光强度信息和相位信息，供体荧光成分的泄漏率和受体荧光成分的泄漏率来确定供体荧光成分的FRET荧光寿命，从而确定FRET荧光效率。

4. 发明申请

US20100312482A1 FRET DETECTION METHOD AND DEVICE 有权
标题翻译： FRET检测方法和设备
公开(公告)号：US20100312482A1
公开(公告)日：2010-12-09
申请号：US12675323
申请日：2007-08-30
申请人： Shigeyuki Nakada , Noriaki Kimura
发明人： Shigeyuki Nakada , Noriaki Kimura
IPC分类号： G06F19/00 , G01N21/64 , G01J1/58
CPC分类号： G01N21/6428 , G01N21/6408 , G01N2021/6441
摘要： When FRET efficiency is measured quantitatively by removing uncertain elements of fluorescence detection information, calibration information prestored in a storage means while including at least the leak rate of donor fluorescence component emitted from a donor molecule, the leak rate of acceptor fluorescence component emitted from an acceptor molecule, and the non-FRET fluorescence lifetime of the donor fluorescence component when FRET is not generated out of the fluorescence of a measurement object sample is acquired. The FRET fluorescence lifetime of the donor fluorescence component is then determined using the intensity information and phase information of fluorescence of the measurement object sample, the leak rate of donor fluorescence component and the leak rate of acceptor fluorescence component, thus determining the FRET fluorescence efficiency.
摘要翻译：当通过消除荧光检测信息的不确定因素，预先存储在存储装置中的校准信息同时至少包括从供体分子发射的供体荧光成分的泄漏率，从受体发射的受体荧光成分的泄漏率，定量测量FRET效率并且获得当测量对象样品的荧光不产生FRET时供体荧光成分的非FRET荧光寿命。然后使用测量对象样品的荧光强度信息和相位信息，供体荧光成分的泄漏率和受体荧光成分的泄漏率来确定供体荧光成分的FRET荧光寿命，从而确定FRET荧光效率。

5. 发明申请

US20100193703A1 FRET DETECTION METHOD AND DEVICE 审中-公开
标题翻译： FRET检测方法和设备
公开(公告)号：US20100193703A1
公开(公告)日：2010-08-05
申请号：US12669619
申请日：2007-08-30
申请人： Noriaki Kimura , Shigeyuki Nakada , Kyouji Doi
发明人： Noriaki Kimura , Shigeyuki Nakada , Kyouji Doi
IPC分类号： G01N21/64
CPC分类号： G01N21/6428 , G01N2021/6419
摘要： When FRET (Fluorescence Resonance Energy Transfer) detection of a large number of samples is performed in a short time for a sample consisting of a donor molecule and an acceptor molecule, the donor molecule is irradiated at first with first laser light used for exciting a donor molecule subjected to intensity modulation at a frequency of f+Δf, the accepter molecule is irradiated with second laser light used for exciting an acceptor molecule subjected to intensity modulation at a frequency of f, and fluorescence emitted from the accepter molecule is received. From a fluorescence signal thus received, a first signal component of fluorescence emitted from the accepter molecule through FRET, and a second signal component of fluorescence emitted from an accepter molecule excited through irradiation with the second laser light are extracted. Phase lags of the first and second signal components thus extracted are then calculated and the presence of generation of FRET is judged based on these phase lags.
摘要翻译：当由供体分子和受体分子构成的样品在短时间内对大量样品进行FRET（荧光共振能量转移）检测时，首先用供激子激发的激光照射供体分子分子以f +＆Dgr; f的频率进行强度调制; f，用用于激发以f频率进行强度调制的受体分子的第二激光照射受体分子，并接收从受体分子发出的荧光。从如此接收到的荧光信号中，提取从受体分子通过FRET发射的荧光的第一信号分量和从通过照射第二激光而激发的受体分子发射的荧光的第二信号分量。然后计算出如此提取的第一和第二信号分量的相位滞后，并且基于这些相位滞后来判断是否存在FRET。

6. 发明授权

US07822558B2 Fluorescence detecting device and fluorescence detecting method 有权
标题翻译：荧光检测装置和荧光检测方法
公开(公告)号：US07822558B2
公开(公告)日：2010-10-26
申请号：US11816244
申请日：2006-02-15
申请人： Noriaki Kimura , Kyoji Doi , Takayoshi Yumii , Takashi Yoshida , Shigeyuki Nakada , Hironori Hayashi , Kazuteru Hoshishima
发明人： Noriaki Kimura , Kyoji Doi , Takayoshi Yumii , Takashi Yoshida , Shigeyuki Nakada , Hironori Hayashi , Kazuteru Hoshishima
IPC分类号： G06F19/00
CPC分类号： G01N21/274 , G01N15/1459 , G01N21/6408 , G01N21/6428 , G01N21/645 , G01N2021/6419 , G01N2021/6421 , G01N2021/6439 , G01N2021/6482
摘要： A fluorescence detecting device irradiates an object to be measured with a laser beam, receives fluorescence generated from the object and processes a fluorescence signal generated when receiving the fluorescence. The device includes a laser light source section outputting the laser beam for irradiating the object, a light receiving section outputting the fluorescence signal of the fluorescence generated by the irradiated object, a light source control section generates a modulation signal having a frequency in order to time-modulate an intensity of the laser beam, and a processing section that calculates a fluorescence relaxation time of the fluorescence of the object based on the fluorescence signal output from the light receiving section. From the detection values acquired by the device including the phase information on the fluorescence, the intensity of the fluorescence is calculated.
摘要翻译：荧光检测装置用激光束照射待测物体，接收从物体产生的荧光，并处理在接收荧光时产生的荧光信号。该装置包括输出用于照射物体的激光束的激光光源部分，输出被照射物体产生的荧光的荧光信号的光接收部分，光源控制部分产生具有频率的调制信号以便时间 - 调制激光束的强度，以及处理部，其基于从光接收部输出的荧光信号，计算对象的荧光的荧光弛豫时间。根据由包括荧光的相位信息的装置获取的检测值，计算荧光强度。

7. 发明申请

US20090012721A1 FLUORESCENCE DETECTING DEVICE AND FLUORESCENCE DETECTING METHOD 有权
标题翻译：荧光检测装置和荧光检测方法
公开(公告)号：US20090012721A1
公开(公告)日：2009-01-08
申请号：US11816244
申请日：2006-02-15
申请人： Noriaki Kimura , Kyoji Doi , Takayoshi Yumii , Takashi Yoshida , Shigeyuki Nakada , Hironori Hayashi , Kazuteru Hoshishima
发明人： Noriaki Kimura , Kyoji Doi , Takayoshi Yumii , Takashi Yoshida , Shigeyuki Nakada , Hironori Hayashi , Kazuteru Hoshishima
IPC分类号： G01N21/64 , G06F19/00
CPC分类号： G01N21/274 , G01N15/1459 , G01N21/6408 , G01N21/6428 , G01N21/645 , G01N2021/6419 , G01N2021/6421 , G01N2021/6439 , G01N2021/6482
摘要： A fluroescene detecting device irradiates an object to be measured with a laser beam, receives fluroescene generated from the object and processes a fluorescence signal generated when receiving the fluroescene. The device includes a laser light source section outputting the laser beam for irradiating the object, a light receiving section outputting the fluorescene signal of the fluorescene generated by the irradiated object, a light source control section generates a modulatioon signal having a frequency in order to time-modulate an intensity of the laser beam, and a processing section that calculates a flurescene relaxation time of the fluorescene of the object based on the fluorescene relaxation time of the fluorescene of the object based on the fluorescene relaxation time of the fluorescene of the object based on the fluorescene signal output from the light receiving section. From the detection values acquired by the device including the phase information on the fluorescene, the intensity of the fluorescene is calculated.
摘要翻译：氟草醚检测装置用激光束照射待测物体，接收从物体产生的氟草醚，并处理接收氟草醚时产生的荧光信号。该装置包括输出用于照射物体的激光束的激光光源部分，输出由照射物体产生的荧光的荧光信号的光接收部分，光源控制部分产生具有频率的模数信号以便时间 - 调制激光束的强度，以及处理部，其基于物体的荧光的荧光弛豫时间，基于物体的荧光的荧光弛豫时间，计算物体的荧光的荧光弛豫时间在从光接收部分输出的荧光信号上。从包括荧光相位信息的装置获取的检测值，计算出荧光的强度。

8. 发明授权

US08885165B2 Fluorescence detecting device and fluorescence detecting method 有权
标题翻译：荧光检测装置和荧光检测方法
公开(公告)号：US08885165B2
公开(公告)日：2014-11-11
申请号：US13145763
申请日：2010-01-15
申请人： Kazuteru Hoshishima , Shigeyuki Nakada
发明人： Kazuteru Hoshishima , Shigeyuki Nakada
IPC分类号： G01N21/25 , G01N21/64 , G01N15/14
CPC分类号： G01N21/6408 , G01N15/147 , G01N21/6428 , G01N2021/6419 , G01N2021/6421 , G01N2021/6441
摘要： A fluorescence detecting device receives fluorescence emitted by n kinds of measurement objects within wavelength bands FLk (k is an integer of 1 to n) set so that the fluorescence intensity of fluorescence emitted by a measurement object k becomes higher than that of fluorescence emitted by the other one or more measurement objects, and acquires fluorescent signals corresponding to the wavelength bands FLk (k is an integer of 1 to n). Each of the fluorescent signals is subjected to frequency-down conversion by mixing it with a modulation signal for modulating the intensity of a laser beam Lk (k=1) corresponding to at least one of the wavelength bands FLk to produce fluorescence data including the phase delay and intensity amplitude of the fluorescent signal. The fluorescence data is corrected to calculate the phase delay and a fluorescence relaxation time is calculated using the phase delay.
摘要翻译：荧光检测装置接收被设定的波长范围FLk（k为1〜n的整数）内的n种测量对象发出的荧光，使得测量对象k发射的荧光的荧光强度比由其他一个或多个测量对象，并且获取对应于波长带FLk（k为1至n的整数）的荧光信号。通过将每个荧光信号与调制信号进行降频转换，该调制信号用于调制与至少一个波长带FLk相对应的激光束Lk（k = 1）的强度，以产生包括相位的荧光数据荧光信号的延迟和强度幅度。校正荧光数据以计算相位延迟，并使用相位延迟来计算荧光弛豫时间。

9. 发明授权

US08817244B2 Fluorescence measurement device and fluorescence measurement method 有权
标题翻译：荧光测量装置和荧光测量方法
公开(公告)号：US08817244B2
公开(公告)日：2014-08-26
申请号：US13521684
申请日：2011-01-13
申请人： Kazuteru Hoshishima , Shigeyuki Nakada
发明人： Kazuteru Hoshishima , Shigeyuki Nakada
IPC分类号： G01N21/00 , G01N21/53 , G01J3/02 , G01N21/64 , G01N15/14
CPC分类号： G01J3/02 , G01N15/1429 , G01N15/1459 , G01N21/53 , G01N21/645 , G01N2021/6491
摘要： Disclosed herein is a fluorescence measuring apparatus capable of more accurately measuring fluorescence emitted when an object to be measured is irradiated with laser light.The apparatus for measuring fluorescence emitted when an object to be measured is irradiated with laser light includes: a laser light source that irradiates the object to be measured with laser light; a first light-receiving unit that receives scattered light emitted when the object to be measured is irradiated with the laser light; a second light-receiving unit that receives fluorescence emitted when the object to be measured is irradiated with the laser light; and a signal processing unit that assigns a weight to a signal of the fluorescence received by the second light-receiving unit depending on an intensity of the scattered light received by the first light-receiving unit.
摘要翻译：本发明公开了一种荧光测定装置，其能够更精确地测定在用激光照射被测定物时发出的荧光。当用激光照射待测量物体时发出的荧光的测量装置包括：用激光照射待测物体的激光光源; 第一光接收单元，其在用激光照射被测量物体时发射的散射光; 第二光接收单元，其接收用激光照射待测量对象时发出的荧光; 以及信号处理单元，其根据由第一光接收单元接收的散射光的强度，分配由第二光接收单元接收的荧光的信号的权重。

10. 发明授权

US08330124B2 Fluorescence detection device using intensity-modulated laser light and fluorescence detection method 有权
标题翻译：荧光检测装置采用强度调制激光和荧光检测方法
公开(公告)号：US08330124B2
公开(公告)日：2012-12-11
申请号：US13119702
申请日：2009-09-16
申请人： Kyouji Doi , Shigeyuki Nakada , Hironori Hayashi , Kazuteru Hoshishima
发明人： Kyouji Doi , Shigeyuki Nakada , Hironori Hayashi , Kazuteru Hoshishima
IPC分类号： G01N21/64 , G01J1/58
CPC分类号： G01N21/6408 , G01N15/1429 , G01N15/1459
摘要： A fluorescence detection device for a flow site meter emits laser light intensity-modulated in accordance with a modulation signal and acquires a fluorescent signal of fluorescence emitted from a measurement object that passes through a measurement point of the laser light. The fluorescence detection device generates, separately from the modulation signal, a reference signal having a frequency different from a frequency of the modulation signal and a phase in synchronization with a phase of the modulation signal. The fluorescence detection device determines a fluorescent relaxation time of the measurement object from the fluorescent signal by using the reference signal.
摘要翻译：流场现场仪的荧光检测装置根据调制信号发射激光强度调制，并获取从通过激光测量点的测量对象发射的荧光的荧光信号。荧光检测装置与调制信号分离地产生具有与调制信号的频率不同的频率的参考信号和与调制信号的相位同步的相位。荧光检测装置通过使用参考信号从荧光信号确定测量对象的荧光弛豫时间。

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