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1. 发明授权

US4346173A Process for the production of acyl-Coenzyme A oxidase 失效
标题翻译：制备酰基辅酶A氧化酶的方法
公开(公告)号：US4346173A
公开(公告)日：1982-08-24
申请号：US201123
申请日：1980-10-27
申请人： Shigeru Ikuta , Shigeyuki Imamura , Hidehiko Ishikawa , Kazuo Matsuura , Masaki Takada , Hideo Misaki
发明人： Shigeru Ikuta , Shigeyuki Imamura , Hidehiko Ishikawa , Kazuo Matsuura , Masaki Takada , Hideo Misaki
IPC分类号： C12N9/02
CPC分类号： C12Y103/03006 , C12N9/001 , C12R1/06 , C12R1/645 , C12R1/66 , C12R1/865 , Y10S435/83 , Y10S435/911 , Y10S435/913 , Y10S435/942
摘要： A process for the production of acyl-Coenzyme A oxidase, comprises culturing an acyl-Coenzyme A-oxidase-producing microorganism belonging to genus Macrophomina, genus Cladosporium, genus Aspergillus, genus Monascus, genus Saccharomyces or genus Arthrobacter in a nutrient medium, and isolating the thus-formed acyl-CoA oxidase therefrom. The preferred species of microorganism are Macrophomina phaseoli ATCC 14383, Cladosporium resinae IFO 6367, Aspergillus candidus M-4815 FERM-P No. 5226, Monascus sp. M-4800 FERM-P No. 5225, Saccharomyces cerevisiae Y 0036 FERM-P No. 5174, and Arthrobacter sp. B-720 FERM-P No. 5224, respectively.
摘要翻译：一种制备酰基辅酶A氧化酶的方法，包括在营养培养基中培养属于巨噬细胞，枝孢霉属，曲霉属，红曲霉属，酿酒酵母属或节杆菌属的酰基辅酶A氧化酶微生物，由此形成的酰基辅酶A氧化酶。微生物的优选种类是Macrophomina phaseoli ATCC 14383，Cladosporium resinae IFO 6367，Aspergillus candidus M-4815 FERM-P No.5226，Monascus sp。 M-4800 FERM-P No.5225，酿酒酵母Y 0036 FERM-P No.5174和节杆菌属（Arthrobacter sp。 B-720 FERM-P No. 5224。

2. 发明授权

US4135980A Choline oxidase 失效
标题翻译：胆固醇氧化酶
公开(公告)号：US4135980A
公开(公告)日：1979-01-23
申请号：US789990
申请日：1977-04-22
申请人： Shigeru Ikuta , Yoshifumi Horiuchi , Hideo Misaki , Kazuo Matsuura , Shigeyuki Imamura , Naoki Muto
发明人： Shigeru Ikuta , Yoshifumi Horiuchi , Hideo Misaki , Kazuo Matsuura , Shigeyuki Imamura , Naoki Muto
IPC分类号： C12N9/04 , C12N9/16 , C12Q1/32 , C12Q1/44 , C12Q1/46 , C12D13/10 , G01N31/14
CPC分类号： C12N9/0006 , C12N9/16 , C12Q1/32 , C12Q1/44 , C12Q1/46 , C12R1/06 , C12R1/465 , Y10S435/83
摘要： A novel enzyme choline oxidase is produced by culturing a microorganism belonging to the genus Arthrobacter, and particularly the species Arthrobacter globiformis B-0577 FERM-P No. 3518, NRRL B-11097, and isolating the choline oxidase thus produced from the culture medium. Choline oxidase is useful for the determination of choline or betaine aldehyde in a sample by mixing the new product with the sample and then measuring the generated hydrogen peroxide, betaine or consumed oxygen.

3. 发明授权

US4491631A Assay method for lipid component, assay composition, and process for production of enzyme used therefor 失效
标题翻译：用于脂质组分的测定方法，测定组合物和用于生产酶的方法
公开(公告)号：US4491631A
公开(公告)日：1985-01-01
申请号：US392010
申请日：1982-06-25
申请人： Shigeyuki Imamura , Hideo Misaki , Hidehiko Ishikawa , Kazuo Matsuura
发明人： Shigeyuki Imamura , Hideo Misaki , Hidehiko Ishikawa , Kazuo Matsuura
IPC分类号： C12N9/02 , C12N9/10 , C12N9/88 , C12Q1/25 , C12Q1/00 , C12N9/00 , C12N9/04 , C12Q1/26 , C12Q1/32 , C12Q1/42 , C12Q1/44 , C12Q1/46 , C12Q1/48 , C12Q1/60 , C12R1/38
CPC分类号： C12Y103/99003 , C12N9/001 , C12N9/1029 , C12N9/88 , C12Q1/25 , Y10S435/874
摘要： An enzyme having enoyl-CoA hydratase activity, 3-hydroxyacyl-CoA dehydrogenase activity and 3-ketoacyl-CoA thiolase activity, all in the same enzyme, is produced by culturing the microorganism strain Pseudomonas fragi B-0771 FERM-P No. 5701, and isolating the enzyme thus produced from the culture medium. Such an enzyme is useful in an assay method for a fatty acid component in a sample, which fatty acid is originally present in the sample or is liberated from a fatty acid ester in the sample, comprising:(a) converting the fatty acid to acyl-CoA;(b) converting the thus-produced acyl-CoA to dehydroacyl-CoA;(c) converting the thus-produced dehydroacyl-CoA to hydroxyacyl-CoA;(d) converting the thus-produced hydroxyacyl-CoA to ketoacyl-CoA;(e) converting the thus-produced ketoacyl-CoA to acyl-CoA; and measuring the detectable changes in the reaction mixture.A composition suitable for such lipid assay comprisesATP or GTP,CoASH,NAD,acyl-CoA synthetase activity,acyl-CoA oxidase activity,enoyl-CoA hydratase activity,3-hydroxyacyl-CoA dehydrogenase activity, and3-ketoacyl-CoA thiolase activity,wherein the last three activities are supplied by the new multi-active enzyme.
摘要翻译：通过培养微生物菌株Pseudomonas fragi B-0771 FERM-P No. 5701制备具有烯酰辅酶A水合酶活性，3-羟基酰基-CoA脱氢酶活性和3-酮酰基-CoA硫羟酸酶活性的酶，全部在同一酶中，并从培养基中分离由此产生的酶。这样的酶可用于样品中的脂肪酸组分的测定方法，该脂肪酸最初存在于样品中或从样品中的脂肪酸酯中释放出来，其包括：（a）将脂肪酸转化为酰基 -CoA; （b）将由此产生的酰基辅酶A转化为脱氢酰基-CoA; （c）将由此产生的脱氢酰基-CoA转化为羟基酰基-CoA; （d）将由此产生的羟基酰基CoA转化为酮酰基-CoA; （e）将由此产生的酮酰基-CoA转化成酰基-CoA; 并测量反应混合物中可检测的变化。适用于这种脂质测定的组合物包括ATP或GTP，CoASH，NAD，酰基-CoA合成酶活性，酰基-CoA氧化酶活性，烯酰辅酶A水合酶活性，3-羟基酰基-CoA脱氢酶活性和3-酮酰基-CoA硫解酶活性其中最后三种活性由新的多活性酶提供。

4. 发明授权

US5126246A Reagent for analysis of triglycerides and analysis using the same 失效
标题翻译：用于分析甘油三酸酯的试剂和使用其分析
公开(公告)号：US5126246A
公开(公告)日：1992-06-30
申请号：US435003
申请日：1989-11-09
申请人： Shigeyuki Imamura , Mamoru Takahashi , Hideo Misaki , Kazuo Matsuura
发明人： Shigeyuki Imamura , Mamoru Takahashi , Hideo Misaki , Kazuo Matsuura
IPC分类号： G01N33/92 , C12Q1/44 , C12Q1/48 , C12Q1/61
CPC分类号： C12Q1/48 , C12Q1/44 , Y10S435/81 , Y10S435/832 , Y10S436/815 , Y10S530/825 , Y10S530/83
摘要： Reagent for analysis of triglycerides contained in blood serum is provided, which comprises lipases and monoglyceride lipases capable of acting on monoglycerides having substrate specificity and capable of catalyzing the following enzymatic reaction: monoglyceride+H.sub.2 O.fwdarw.glycerol+fatty acids. The glycerol or fatty acids are measured to learn an amount of the triglycerides or fatty acid by any known analytical method.
摘要翻译：提供了用于分析血清中含有的甘油三酯的试剂，其包含能够作用于具有底物特异性并能够催化以下酶促反应的单酸甘油酯的脂肪酶和甘油单酯脂肪酶：单酸甘油酯+ H 2 O->甘油+脂肪酸。测量甘油或脂肪酸，以通过任何已知的分析方法学习一定量的甘油三酯或脂肪酸。

5. 发明授权

US4921786A Novel NAD synthetase, assay method using said novel NAD synthetase and a process for production thereof 失效
标题翻译：新型NAD合成酶，使用所述新型NAD合成酶的测定方法及其生产方法
公开(公告)号：US4921786A
公开(公告)日：1990-05-01
申请号：US94813
申请日：1987-09-10
申请人： Mamoru Takahashi , Hideo Misaki , Shigeyuki Imamura , Kazuo Matsuura
发明人： Mamoru Takahashi , Hideo Misaki , Shigeyuki Imamura , Kazuo Matsuura
IPC分类号： C12N9/00 , C12P1/00 , C12P3/00
CPC分类号： C12N9/93 , C12P1/00 , C12P3/00 , C12R1/07
摘要： A novel NAD synthetase is produced by culturing a broth of Bacillus stearothermophilus H-804 FERM BP-1408. This new enzyme selectively catalyzes the reaction ##STR1## without catalyzing the reaction ##STR2## The enzyme uses ammonia or ammonium ion as a substrate, but does not use either glutamine or asparagine. Also disclosed is an assay method using the enzyme, for any one of ATP, deamide-NAD, ammonia or ammonium ion in a specimen to be assayed.

6. 发明授权

US5079158A Novel monoglyceride lipase and its production process. 失效
标题翻译：新型单糖脂及其生产工艺。
公开(公告)号：US5079158A
公开(公告)日：1992-01-07
申请号：US171272
申请日：1988-03-21
申请人： Shigeyuki Imamura , Mamoru Takahashi , Hideo Misaki , Kazuo Matsuura
发明人： Shigeyuki Imamura , Mamoru Takahashi , Hideo Misaki , Kazuo Matsuura
IPC分类号： C12N9/18 , C12Q1/44 , C12R1/07
CPC分类号： C12N9/18 , C12Q1/44 , Y10S435/832
摘要： Disclosed herein is a novel monoglyceride lipase at least capable of catalyzing an enzymatic reaction of the following equation (a) and as substrate specificity, capable of acting on monoglyceride but incapable of acting on diglyceride and triglyceride:(a) Monoglyceride+H.sub.2 O.fwdarw.Glycerol+Fatty acidThe monoglyceride lipase is produced by culturing a specific monoglyceride lipase producing microorganism of Bacillus and then collecting the monoglyceride lipase from the resulting culture. A method is also disclosed for the analysis of a monoglyceride-containing sample solution. The monoglyceride lipase is caused to act on the sample solution upon measurement of the monoglyceride in the sample solution. Either one of glycerol and the fatty acid formed in accordance with the equation (a) is then measured.

7. 发明授权

US4770997A Thermostable bilirubin oxidase and production process thereof 失效
标题翻译：热稳定性胆红素氧化酶及其制备方法
公开(公告)号：US4770997A
公开(公告)日：1988-09-13
申请号：US820011
申请日：1986-01-21
申请人： Eiichi Yoshino , Shigeyuki Imamura , Kazuo Matsuura , Hideo Misaki
发明人： Eiichi Yoshino , Shigeyuki Imamura , Kazuo Matsuura , Hideo Misaki
IPC分类号： C12N9/06 , C12N9/02 , C12Q1/26 , C12R1/10
CPC分类号： C12Y103/03005 , C12N9/001 , C12Q1/26 , Y10S435/836
摘要： Thermostable bilirubin oxidase having substrate specificity to at least bilirubin and capable of catalyzing a reaction in which biliverdin and water are formed from bilirubin and oxygen. It can be produced by culturing a bilirubin oxidase producing micro-organism belonging to the genus Bacillus, for example, Bacillus licheniformis and then preparing the resultant bilirubin oxidase from the cultured broth.
摘要翻译：具有对至少胆红素具有底物特异性并且能够催化由胆红素和氧形成胆碱和水的反应的热稳定性胆红素氧化酶。可以通过培养属于芽孢杆菌属的例如地衣芽孢杆菌的产生胆红素氧化酶的微生物，然后从培养的肉汤中制备所得到的胆红素氧化酶来制备。

8. 发明授权

US5356790A Highly sensitive assay method for myo-inositol, composition for practicing same, novel myo-inositol dehydrogenase, and process for producing same 失效
标题翻译：肌醇的高灵敏度测定方法，实践相同的组合物，新型肌醇脱氢酶及其制备方法
公开(公告)号：US5356790A
公开(公告)日：1994-10-18
申请号：US106693
申请日：1993-08-16
申请人： Shigeru Ueda , Mamoru Takahashi , Hideo Misaki , Shigeyuki Imamura , Kazuo Matsuura
发明人： Shigeru Ueda , Mamoru Takahashi , Hideo Misaki , Shigeyuki Imamura , Kazuo Matsuura
IPC分类号： C12N9/04 , C12Q1/02 , C12Q1/32 , C12Q1/26 , C07H1/00 , C12N11/00
CPC分类号： C12N9/0006 , C12Q1/02 , C12Q1/32 , C12R1/07 , Y10S436/817
摘要： Myo-inositol in a specimen is assayed by reacting a specimen containing myo-inositol with:a) myo-inositol dehydrogenase using a thio-NADP group or thio-NAD group and an NADP group or NAD group as coenzymes, and which catalyzes a reversible reaction forming myo-inosose from myo-inositol,b ) A.sub.1 andc) B.sub.1to effect a cycling reaction ##STR1## wherein A.sub.1 is a thio-NADP group, thio-NAD group, NADP group or NAD group, A.sub.2 is a reduced form of A.sub.1, when A.sub.1 is a thio-NADP group or thio-NAD group, B.sub.1 is a reduced NADP group or reduced NAD group and when A.sub.1 is an NADP group or NAD group, B.sub.1 is a reduced thio-NADP group or reduced thio-NAD group, and wherein B.sub.2 is an oxidized form of B.sub.1. The change in the amount of A.sub.2 generated or B.sub.1 consumed by the cycling reaction is measured to perform the assay. A composition for performing the assay comprises the above myo-inositol dehydrogenase, as well as the above components A.sub.1 and B.sub.1. The myo-inositol dehydrogenase can be produced by culturing a suitable microorganism belonging to genus Bacillus, particularly Bacillus sp. No. 3 FERM BP-3013.
摘要翻译：通过使含有肌醇的样品与以下物质反应来测定样品中的肌醇：a）使用硫代NADP基团或硫代NAD基团和NADP基团或NAD基团作为辅酶的肌醇脱氢酶，并催化可逆的由肌球蛋白形成肌内单糖的反应，b）A1和c）B1进行循环反应，其中A1是硫代NADP基团，硫代NAD基团，NADP基团或NAD基团，A2是还原形式的A1，当A1是硫代NADP基团或硫代NAD基团时，B1是还原的NADP基团或还原的NAD基团，当A1是NADP基团或NAD基团时，B1是还原的硫代NADP基团或还原的硫代 - NAD基，其中B2是B1的氧化形式。测量由循环反应消耗的A2产生量或B1的量的变化以进行测定。用于进行测定的组合物包含上述肌醇脱氢酶，以及上述组分A1和B1。肌醇脱氢酶可以通过培养属于芽孢杆菌属（Bacillus），特别是芽孢杆菌属（Bacillus sp。）的合适的微生物来产生。 3号FERM BP-3013。

9. 发明授权

US4385112A Nucleoside oxidase and process for making same, and process and kit for using same 失效
标题翻译：核苷氧化酶及其制备方法，及其使用方法和试剂盒
公开(公告)号：US4385112A
公开(公告)日：1983-05-24
申请号：US292154
申请日：1981-08-12
申请人： Hideo Misaki , Shigeru Ikuta , Kazuo Matsuura
发明人： Hideo Misaki , Shigeru Ikuta , Kazuo Matsuura
IPC分类号： C07H19/04 , C07H21/00 , C12N9/04 , C12P19/28 , C12P19/38 , C12Q1/26 , C12Q1/28 , C12N9/06 , C12P7/40 , C12P13/00 , C12Q1/34 , C12Q1/42 , C12Q1/68 , C12R1/40
CPC分类号： C07H21/00 , C07H19/04 , C12N9/0006 , C12P19/28 , C12P19/38 , C12Q1/26 , C12Q1/28 , C12Y101/03028 , Y10S435/81 , Y10S435/877
摘要： A microorganism strain B-0781 belonging to the genus Pseudomonas isolated from a soil sample from an onion field in Japan, produces a novel enzyme nucleoside oxidase having substrate specificity on various nucleosides and enzyme action to catalyze enzymatic reactions involving various nucleosides. The novel nucleoside oxidase is produced by culturing a nucleoside-oxidase-producing microorganism belonging to the genus Pseudomonas in a nutrient medium containing assimilable carbon and nitrogen and inorganic salt, and isolating the thus-formed nucleoside oxidase from the cultured cells. Various nucleoside-5'-carboxylic acids can be produced, by incubating the novel nucleoside oxidase with a nucleoside having a 5'-hydroxymethyl group, in an aqueous medium under aerobic conditions, and isolating the thus-formed nucleoside-5'-carboxylic acid from the incubation medium. Assay methods for nucleosides in liquid samples are provided, which comprise incubating the sample with nucleoside oxidase, thereby consuming oxygen and generating hydrogen peroxide and nucleoside-5'-carboxylic acid by acting on nucleoside, and quantitatively determining consumed oxygen or liberated hydrogen peroxide. A kit for nucleoside assay is provided, which contains the recited ingredients.
摘要翻译：属于从日本洋葱田土壤样品中分离出的假单胞菌属的微生物菌株B-0781产生了对各种核苷具有底物特异性和酶作用的新的酶核苷氧化酶，以催化涉及各种核苷的酶反应。通过在含有可同化碳，氮和无机盐的营养培养基中培养属于假单胞菌属的核苷 - 氧化酶产生微生物，并从培养细胞中分离由此形成的核苷氧化酶，从而产生新的核苷氧化酶。通过在有氧条件下在含水介质中孵育新的核苷氧化酶与具有5'-羟甲基的核苷，可以制备各种核苷-5'-羧酸，并分离由此形成的核苷-5'-羧酸从培养基中。提供液体样品中核苷的测定方法，其包括将样品与核苷氧化酶孵育，由此消耗氧并通过作用于核苷产生过氧化氢和核苷-5'-羧酸，并定量测定消耗的氧气或释放的过氧化氢。提供了用于核苷测定的试剂盒，其包含所述的成分。

10. 发明授权

US5162201A Analytical method making use of monoglyceride lipase 失效
标题翻译：使用单甘油脂肪酶的分析方法
公开(公告)号：US5162201A
公开(公告)日：1992-11-10
申请号：US668169
申请日：1991-03-12
申请人： Shigeyuki Imamura , Mamoru Takahasi , Hideo Misaki , Kazuo Matsuura
发明人： Shigeyuki Imamura , Mamoru Takahasi , Hideo Misaki , Kazuo Matsuura
IPC分类号： C12N9/18 , C12Q1/44
CPC分类号： C12Q1/44 , C12N9/18
摘要： Disclosed herein is a novel monoglygeride lipase at least capable of catalyzing an enzymatic reaction of the following equation (a) and as substrate specificity, capable of acting on monoglyceride but incapable of acting on diglyceride and triglyceride:Monoglyceride+H.sub.2 O.fwdarw.Glycerol+Fatty acid (a)The monoglyceride lipase is produced by culturing a specific monoglyceride lipase producing microorganism of Bacillus and then collecting the monoglyceride lipase from the resulting culture. A method is also disclosed for the analysis of a monoglyceride-containing sample solution. The monoglyceride lipase is caused to act on the sample solution upon measurement of the monoglyceride in the sample solution. Either one of glycerol and the fatty acid formed in accordance with the equation (a) is then measured.
摘要翻译：本文公开了一种新颖的单甙脂肪酶，其至少能够催化以下等式（a）的酶反应和作为底物特异性，其能够作用于甘油单酯但不能作用于甘油二酯和甘油三酸酯：单甘油酯+ H 2 O→甘油+脂肪酸（a）单酸甘油酯脂肪酶是通过培养产生芽孢杆菌的特定单酸甘油酯脂肪酶微生物，然后从得到的培养物中收集单甘油脂肪酶产生的。还公开了用于分析含单甘油酯的样品溶液的方法。当测量样品溶液中的单甘油酯时，使甘油单酯脂肪酶作用于样品溶液。然后测量根据方程（a）形成的甘油和脂肪酸中的任一种。

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