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    • 2. 发明授权
    • Neuron-specific retrograde transport vector
    • 神经元特异性逆行运输载体
    • US09006411B2
    • 2015-04-14
    • US13988973
    • 2011-11-25
    • Kazuto Kobayashi
    • Kazuto Kobayashi
    • C07K14/005A01N63/00A01N65/00C12N15/86A61K48/00
    • C12N15/86A61K48/0058C07K14/005C07K2319/00C07K2319/03C12N2740/16045C12N2740/16071C12N2760/20122C12N2760/20222C12N2810/6081
    • The present invention provides a lentiviral vector system having a higher titer, while sustaining an excellent retrograde transport ability, particularly, in the brain.The present invention also provides a kit for preparing a retrograde transport viral vector comprising: (1) a packaging plasmid containing the gag gene and the pol gene of HIV-1; (2) a packaging plasmid containing an accessory gene of HIV-1; (3) a transfer plasmid containing an target gene (a transgene); and (4) an envelope plasmid containing, as an envelope gene, a gene encoding a fused polypeptide comprising a fused extracellular domain consisting of the N-terminal region of an extracellular domain of rabies virus glycoprotein (RV-G) and the C-terminal region of an extracellular domain of vesicular stomatitis virus glycoprotein (VSV-G), a transmembrane domain of RV-G or VSV-G, and an intracellular domain of VSV-G, and the like.
    • 本发明提供了具有较高滴度的慢病毒载体系统,同时维持优异的逆行转运能力,特别是在脑中。 本发明还提供了用于制备逆行转运病毒载体的试剂盒,其包含:(1)含有HIV-1的gag基因和pol基因的包装质粒; (2)含有HIV-1辅助基因的包装质粒; (3)含有靶基因(转基因)的转移质粒; (4)包含作为包膜基因的包含编码融合多肽的基因的包膜质粒,所述融合多肽包含由狂犬病病毒糖蛋白(RV-G)的细胞外结构域的N末端区域构成的融合细胞外结构域和C-末端 水泡性口炎病毒糖蛋白(VSV-G)的细胞外结构域,RV-G或VSV-G的跨膜结构域和VSV-G的细胞内结构域等。
    • 3. 发明授权
    • Expansion valve
    • 膨胀阀
    • US08806880B2
    • 2014-08-19
    • US12680991
    • 2008-09-18
    • Kazuto KobayashiTakashi MogiEiji Fukuda
    • Kazuto KobayashiTakashi MogiEiji Fukuda
    • F25B41/06
    • F25B41/062F16K27/02F25B2341/0683F25B2500/01
    • An expansion valve for reducing the quantity of material required for the valve body by contriving the arrangement of bolt fitting grooves. In the expansion valve (20), displacement of a valve member driver (36) provided in the valve body (21) is transmitted to a valve member by a valve member drive rod (36f) and the valve opening is altered to control the flow rate of the refrigerant. The valve body (21) consists of a body upper portion (40) and a body lower portion (41) narrower than the body upper portion (40), and bolt fitting grooves (43, 43) opening in the flank of the valve body (21), preferably opening obliquely downward, are formed at the boundary (42) of the body upper portion (40) and the body lower portion (41). With such a layout, the material required at the boundary (42) can be reduced furthermore and the quantity of material required for the valve body (21) can be reduced furthermore, as a result. Since the opening width (minimum spatial dimension) w is smaller than the diameter of bolt, a seating surface is assured sufficiently around the bolt fitting groove (43) and the bolt does not drop off from the bolt fitting groove (43).
    • 一种膨胀阀,用于通过设计螺栓安装槽来减少阀体所需的材料的数量。 在膨胀阀(20)中,设置在阀体(21)中的阀构件驱动器(36)的位移通过阀构件驱动杆(36f)传递到阀构件,并且改变阀开度以控制流量 制冷剂的比例。 阀体(21)由主体上部(40)和比主体上部(40)窄的主体下部(41)构成,在阀体的侧面开口的螺栓嵌合槽(43,43) (21),优选地向下倾斜地开口,形成在主体上部(40)和主体下部(41)的边界(42)处。 通过这样的布局,可以进一步减少在边界(42)处所需的材料,并且可以进一步降低阀体(21)所需的材料量。 由于开口宽度(最小空间尺寸)w小于螺栓的直径,因此在螺栓配合槽(43)周围充分确保就座面,并且螺栓不会从螺栓配合槽(43)脱落。
    • 5. 发明授权
    • Manufacturing method for bearing outer ring
    • 轴承外圈的制造方法
    • US08424208B2
    • 2013-04-23
    • US12522311
    • 2008-01-16
    • Kazuto KobayashiHiroshi Koyama
    • Kazuto KobayashiHiroshi Koyama
    • B21D53/10B21J5/08F16C33/58
    • B23P15/003B21H1/12B21J1/00B21J1/04B21J5/02B21J5/10B21K1/04B21K21/02F16C19/184F16C33/62F16C33/64Y10T29/49636Y10T29/49643Y10T29/49689
    • Provided is a manufacturing method of an outer ring (3) of a back-to-back arrangement double-row angular ball bearing from a raw material (10) comprising a upsetting (A to B), a backward extrusion process (C to D), a punching (D to E), a rolling (E to F) and a finishing, wherein an outer diameter of a first intermediate material (11a) produced by the upsetting is equal to or smaller than an inside diameter of an inner periphery large diameter portion (18) of the inner circumference of a die (13) used in the backward extrusion process and larger than an inside diameter of an inner periphery small portion (19) of the die (13), and in a condition with the first intermediate material (11a) sitting on the inner periphery inclined portion (20) of the die (13), the intermediate material (11a) is pushed toward a base of the die (13) by a punch (14), to thereby the middle metal material (29) with the highest cleanliness of the raw material (10) is exposed to the two outer ring raceways (2) of the outer ring (3).
    • 本发明提供从包括镦粗(A〜B),向后挤压工序(C〜D)的原料(10)的背靠背排列双列角球轴承的外圈(3)的制造方法 ),冲压(D〜E),轧制(E〜F)和精加工,其中通过镦锻制造的第一中间材料(11a)的外径等于或小于内周的内径 在向后挤压过程中使用的模具(13)的内周的大直径部分(18)大于模具(13)的内周小部分(19)的内径,并且在 第一中间材料(11a)位于模具(13)的内周倾斜部分(20)上,中间材料(11a)通过冲头(14)被推向模具(13)的基部,由此 原料(10)清洁度最高的中间金属材料(29)暴露于t的两个外圈滚道(2) 他外圈(3)。
    • 8. 发明授权
    • ENUM system, ENUM client apparatus and method for communicating using ENUM client apparatus
    • ENUM系统,ENUM客户端设备和使用ENUM客户端设备进行通信的方法
    • US08149819B2
    • 2012-04-03
    • US11166236
    • 2005-06-27
    • Kazuto KobayashiAkira Miyajima
    • Kazuto KobayashiAkira Miyajima
    • G06F15/16H04L12/66
    • H04M7/006
    • An ENUM system includes a Web server and an ENUM server. The Web server stores first NAPTR records corresponding to an ENUM client and another ENUM client on an IP network. The ENUM server stores second NAPTR records in which first URIs are associated with predetermined information for being linked to the Web server. In the ENUM system, the ENUM client transmits, to the ENUM server, a query for the second NAPTR record of the another ENUM client. The ENUM client then transmits, to the Web server, a request for access to the first URI included in the received second NAPTR record, and obtains the first NAPTR record corresponding to the another ENUM client.
    • ENUM系统包括Web服务器和ENUM服务器。 Web服务器在IP网络上存储对应于ENUM客户端和另一个ENUM客户端的第一个NAPTR记录。 ENUM服务器存储第二个NAPTR记录,其中第一个URI与预定的信息相关联,以链接到Web服务器。 在ENUM系统中,ENUM客户端向ENUM服务器发送另一个ENUM客户端的第二个NAPTR记录的查询。 然后,ENUM客户端向Web服务器发送访问包括在接收的第二NAPTR记录中的第一URI的请求,并且获得与另一ENUM客户端对应的第一NAPTR记录。
    • 10. 发明授权
    • IP telephone apparatus, ENUM server, and calling method via the internet
    • IP电话设备,ENUM服务器和通过互联网的呼叫方法
    • US07957367B2
    • 2011-06-07
    • US11140907
    • 2005-06-01
    • Kazuto KobayashiAkira Miyajima
    • Kazuto KobayashiAkira Miyajima
    • H04L12/66
    • H04L61/157H04L29/1216H04M3/42042H04M7/0057H04M7/006
    • An ENUM server stores NAPTR records including PSTN telephone numbers and the corresponding IP telephone numbers of a source IP telephone apparatus and a destination IP telephone apparatus. When the ENUM server receives an ENUM query from the source IP telephone apparatus, the ENUM query setting a domain name based on the PSTN telephone number, the ENUM server searches for a NAPTR record corresponding to the domain name and returns, to the source IP telephone apparatus, an ENUM response including the NAPTR record. When receiving the ENUM response from the ENUM server, the source IP telephone apparatus retrieves the IP telephone number of the destination IP telephone apparatus, the IP telephone number being specified in the NAPTR record contained in the ENUM response, and starts a call connection sequence with the destination IP telephone apparatus.
    • ENUM服务器存储包括PSTN电话号码和源IP电话设备和目的IP电话设备的相应IP电话号码的NAPTR记录。 当ENUM服务器从源IP电话设备接收到ENUM查询时,ENUM查询根据PSTN电话号码设置域名,ENUM服务器搜索与域名对应的NAPTR记录,并返回到源IP电话 设备,包括NAPTR记录的ENUM响应。 当从ENUM服务器接收到ENUM响应时,源IP电话设备检索目的地IP电话设备的IP电话号码,该IP电话号码在ENUM响应中包含的NAPTR记录中指定,并开始一个呼叫连接序列, 目的IP电话设备。