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    • 3. 发明申请
    • Compositions and Methods for Protein Production
    • 蛋白质生产的组成和方法
    • US20130084641A1
    • 2013-04-04
    • US13708479
    • 2012-12-07
    • Shib Sankar BasuKristern Kamerath DangKasimalai AzhakanandamThomas Z. McNeillSheng Guo
    • Shib Sankar BasuKristern Kamerath DangKasimalai AzhakanandamThomas Z. McNeillSheng Guo
    • C12N15/10
    • C12N15/1089C12N15/1034
    • The present invention provides a method of selecting a mRNA for production of a polypeptide of interest comprising: a) producing an array of individual mRNA sequences comprising different nucleotide sequences encoding the polypeptide of interest; b) determining one or more or two or more of the following parameters for each individual mRNA sequence of (a): (i) minimum free energy (MFE) RNA secondary structure; (ii) ensemble free energy (EFE); (iii) frequency of the minimum free energy (FMFE) RNA secondary structure in a thermodynamic ensemble; and (iv) ensemble diversity (ED); c) ranking the individual mRNA sequences of the array according the parameters determined in step (b); and d) selecting a mRNA sequence from the ranked array of step (c), wherein the selected mRNA produces the polypeptide of interest. The present invention further provides a method of selecting a mRNA for enhanced and reduced production of a polypeptide of interest.
    • 本发明提供了选择产生感兴趣多肽的mRNA的方法,包括:a)产生包含编码目的多肽的不同核苷酸序列的单个mRNA序列的阵列; b)确定(a)的每个单独mRNA序列的一个或多个或两个或更多个以下参数:(i)最小自由能(MFE)RNA二级结构; (ii)合奏自由能(EFE); (iii)热力学集合中最小自由能(FMFE)RNA二级结构的频率; 和(iv)集体多样性(ED); c)根据步骤(b)中确定的参数对阵列的各个mRNA序列进行排序; 和d)从步骤(c)的排列阵列中选择mRNA序列,其中所选择的mRNA产生感兴趣的多肽。 本发明还提供了选择用于增强和减少感兴趣的多肽的产生的mRNA的方法。