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1. 发明授权

US09556449B2 Methods of increasing yield and stress tolerance in a plant by decreasing the activity of a trehalose-6-phosphate phosphatase 有权
标题翻译：通过降低海藻糖-6-磷酸磷酸酶的活性来增加植物的产量和胁迫耐受性的方法
公开(公告)号：US09556449B2
公开(公告)日：2017-01-31
申请号：US14232912
申请日：2012-07-16
申请人： Shib Basu , Jonathan Cohn , Michael Nuccio
发明人： Shib Basu , Jonathan Cohn , Michael Nuccio
IPC分类号： C12N15/82 , C12N9/16
CPC分类号： C12N15/8273 , C12N9/16 , C12N15/8261
摘要： The present invention relates generally to the field of molecular biology and regards various polynucleotides, polypeptides and methods that may be employed to enhance yield in transgenic plants. Specifically the transgenic plants may exhibit any one of the traits consisting of increased yield, increased tolerance to abiotic stress, increased cell growth and increased nutrient use efficiency.
摘要翻译：本发明一般涉及分子生物学领域，并涉及可用于增加转基因植物产量的各种多核苷酸，多肽和方法。具体地说，转基因植物可以表现出任何一个性状，其特征包括增加的产量，增加的对非生物胁迫的耐受性，增加的细胞生长和增加的营养利用效率。

2. 发明申请

US20100071092A1 METHODS AND GENETIC CONSTRUCTS FOR MODIFICATION OF LIGNIN COMPOSITION OF CORN COBS 有权
标题翻译：方法和遗传修饰的修饰木瓜组合物
公开(公告)号：US20100071092A1
公开(公告)日：2010-03-18
申请号：US12549783
申请日：2009-08-28
申请人： Royston E. Carter , John Steffens , Michael Nuccio , Lawrence M. Lagrimini
发明人： Royston E. Carter , John Steffens , Michael Nuccio , Lawrence M. Lagrimini
IPC分类号： C12N15/82 , A23K1/00 , C12P1/00
CPC分类号： C12N15/8255 , A23K10/00 , C12N15/8226 , C12N15/827 , C12P7/10 , Y02E50/16
摘要： Expression cassettes causing specific regulatory control of transgene expression in plants, wherein the expression cassettes include regulatory sequences from the MADS gene family for expression of recombinant gene products in the reproductive tissue of plants for the purpose of generating maize plants with altered lignin content of the cobs.
摘要翻译：在植物中引起特异性调控对转基因表达的表达盒，其中表达盒包括来自MADS基因家族的调节序列，用于在植物的生殖组织中表达重组基因产物，以产生具有改变的木质素的木质素含量的玉米植物。

3. 发明授权

US07166465B2 Constitutive promoter from Arabidopsis 失效
标题翻译：来自拟南芥的组成型启动子
公开(公告)号：US07166465B2
公开(公告)日：2007-01-23
申请号：US10643676
申请日：2003-08-19
申请人： Terry Thomas , Michael Nuccio , Tzung-Fu Hsieh
发明人： Terry Thomas , Michael Nuccio , Tzung-Fu Hsieh
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N15/8216 , C07K14/415
摘要： The present invention is directed to a promoter derivable from an Arabidopsis ENDO gene. Nucleic acid constructs, vectors, plant cells and transgenic plants comprising the promoter are also provided.
摘要翻译：本发明涉及可从拟南芥ENDO基因得到的启动子。还提供了核酸构建体，载体，植物细胞和包含启动子的转基因植物。

4. 发明授权

US6100450A Seed specific promoters based on arabidopsis genes 失效
标题翻译：基于拟南芥基因的种子特异性启动子
公开(公告)号：US6100450A
公开(公告)日：2000-08-08
申请号：US956182
申请日：1997-10-22
申请人： Terry L. Thomas , Michael Nuccio
发明人： Terry L. Thomas , Michael Nuccio
IPC分类号： C12N15/29 , C12N15/82 , A01H4/00 , A01H5/00 , C12N5/04
CPC分类号： C12N15/8247 , C12N15/8234
摘要： The present invention is directed to 5' regulatory regions of two Arabidopsis seed-specific genes, AtS1 and AtS3. The 5' regulatory regions, or parts thereof, when operably linked to either the coding sequence of a heterologous gene or a sequence complementary to a native plant gene, direct expression of the coding sequence or complementary sequence in a plant seed. The regulatory regions are useful in expression cassettes and expression vectors for the transformation of plants. Also provided are methods of modulating the levels of a heterologous gene such as a fatty acid synthesis or lipid metabolism gene by transforming a plant with the subject expression cassettes and expression vectors.
摘要翻译：本发明涉及两个拟南芥种子特异性基因AtS1和AtS3的5'调节区。当与异源基因的编码序列或与天然植物基因互补的序列可操作地连接时，5'调节区或其部分，在植物种子中直接表达编码序列或互补序列。调节区可用于植物转化的表达盒和表达载体。还提供了通过用本发明的表达盒和表达载体转化植物来调节异源基因水平的方法，例如脂肪酸合成或脂质代谢基因。

5. 发明授权

US08304607B2 Epidermal tissue promoter derived from potato for monocots 有权
标题翻译：来自马铃薯单子叶植物的表皮组织启动子
公开(公告)号：US08304607B2
公开(公告)日：2012-11-06
申请号：US12643187
申请日：2009-12-21
申请人： Michael Nuccio , A. Todd Richmond
发明人： Michael Nuccio , A. Todd Richmond
IPC分类号： C12N15/82 , A01H5/00 , A01H5/10
CPC分类号： C12N15/827 , C12N15/8223
摘要： The presently disclosed subject matter includes nucleic acid sequences and expression cassettes that contain regulatory sequences derived from Solanum tuberosum chitinase C genes, for expression of recombinant gene products in plants. The expression cassette drives high transgene expression in the epidermal (L1) cell layer.
摘要翻译：目前公开的主题包括含有源自马铃薯几丁质酶C基因的调节序列的核酸序列和表达盒，用于在植物中表达重组基因产物。表达盒驱动表皮（L1）细胞层中的高转基因表达。

6. 发明授权

US5614393A Production of .gamma.-linolenic acid by a .DELTA.6-desaturase 失效
标题翻译：通过DELTA 6-去饱和酶生产γ-亚麻酸
公开(公告)号：US5614393A
公开(公告)日：1997-03-25
申请号：US366779
申请日：1994-12-30
申请人： Terry L. Thomas , Avutu S. Reddy , Michael Nuccio , Andrew N. Nunberg , Georges L. Freyssinet
发明人： Terry L. Thomas , Avutu S. Reddy , Michael Nuccio , Andrew N. Nunberg , Georges L. Freyssinet
IPC分类号： A01H1/00 , C12N1/15 , C12N1/21 , C12N5/10 , C12N9/02 , C12N15/00 , C12N15/09 , C12N15/53 , C12N15/74 , C12N15/82 , C12P7/64 , C12R1/01 , C12R1/91
CPC分类号： C12N9/0083 , A01H1/00 , C12N15/74 , C12N15/8247 , C12P7/6427 , C12P7/6436 , C12P7/6472 , C12Y114/19003
摘要： Linoleic acid is converted into .gamma.-linolenic acid by the enzyme .DELTA.6-desaturase. The present invention is directed to isolated nucleic acids comprising the .DELTA.6-desaturase gene. More particularly, the isolated nucleic acid comprises the promoter, coding region and termination regions of the .DELTA.6-desaturase gene. The present invention provides recombinant constructions comprising the .DELTA.6-desaturase coding region in functional combination with heterologous regulatory sequences. The nucleic acids and recombinant constructions of the instant invention are useful in the production of GLA in transgenic organisms.
摘要翻译：亚油酸通过酶DELTA6去饱和酶转化成γ-亚麻酸。本发明涉及包含DELTA 6-去饱和酶基因的分离的核酸。更具体地，分离的核酸包含DELTA 6-去饱和酶基因的启动子，编码区和终止区。本发明提供包含与异源调节序列功能性组合的DELTA 6-去饱和酶编码区的重组构建体。本发明的核酸和重组构建可用于转基因生物中GLA的产生。

7. 发明申请

US20100138955A1 EPIDERMAL TISSUE PROMOTER DERIVED FROM POTATO FOR MONOCOTS 有权
标题翻译：来自POTATO的单体组合的EPIDERMAL TISSUE PROMOTER
公开(公告)号：US20100138955A1
公开(公告)日：2010-06-03
申请号：US12643187
申请日：2009-12-21
申请人： Michael Nuccio , A. Todd Richmond
发明人： Michael Nuccio , A. Todd Richmond
IPC分类号： C12N15/82 , C12N15/11 , A01H5/00 , C12N5/10 , C12N15/55 , C12N15/63
CPC分类号： C12N15/827 , C12N15/8223
摘要： The presently disclosed subject matter includes nucleic acid sequences and expression cassettes that contain regulatory sequences derived from Solanum tuberosum chitinase C genes, for expression of recombinant gene products in plants. The expression cassette drives high transgene expression in the epidermal (L1) cell layer.
摘要翻译：目前公开的主题包括含有源自马铃薯几丁质酶C基因的调节序列的核酸序列和表达盒，用于在植物中表达重组基因产物。表达盒驱动表皮（L1）细胞层中的高转基因表达。

8. 发明申请

US20070006344A1 Regulatory sequences for expressing gene products in plant reproductive tissue 有权
标题翻译：在植物生殖组织中表达基因产物的调节序列
公开(公告)号：US20070006344A1
公开(公告)日：2007-01-04
申请号：US11109594
申请日：2005-04-19
申请人： Michael Nuccio , L. Lagrimini , Moez Meghji
发明人： Michael Nuccio , L. Lagrimini , Moez Meghji
IPC分类号： A01H1/00 , C12N15/82 , C12N5/04
CPC分类号： C12N15/8271 , C12N15/827
摘要： Expression cassettes causing specific regulatory control of transgene expression in plants, wherein the expression cassettes include regulatory sequences from the MADS gene family for expression of recombinant gene products in the reproductive tissue of plants for the purpose of generating abiotic stress tolerant plants.
摘要翻译：表达盒在植物中导致转基因表达的特异性调控，其中表达盒包括来自MADS基因家族的调节序列，用于在植物繁殖组织中表达重组基因产物，以产生非生物胁迫耐受植物。

9. 发明申请

US20050176946A1 Constitutive promoter from Arabidopsis 失效
标题翻译：来自拟南芥的组成型启动子
公开(公告)号：US20050176946A1
公开(公告)日：2005-08-11
申请号：US10643676
申请日：2003-08-19
申请人： Terry Thomas , Michael Nuccio , Tzung-Fu Hsieh
发明人： Terry Thomas , Michael Nuccio , Tzung-Fu Hsieh
IPC分类号： A01H1/00 , C07H21/04 , C07K14/415 , C12N5/04 , C12N9/24 , C12N15/82
CPC分类号： C12N15/8216 , C07K14/415
摘要： The present invention is directed to a promoter derivable from and Arabidopsis ENDO gene. Nucleic acid constructs, vectors, plant cells and transgenic plants comprising the promoter are also provided.
摘要翻译：本发明涉及可衍生自和拟南芥ENDO基因的启动子。还提供了核酸构建体，载体，植物细胞和包含启动子的转基因植物。

10. 发明申请

US20120115734A1 IN SILICO PREDICTION OF HIGH EXPRESSION GENE COMBINATIONS AND OTHER COMBINATIONS OF BIOLOGICAL COMPONENTS 审中-公开
标题翻译：在硅胶中预测高表达基因组合和生物组分的其他组合
公开(公告)号：US20120115734A1
公开(公告)日：2012-05-10
申请号：US12939586
申请日：2010-11-04
申请人： Laura Potter , Michael Nuccio , Rex Dwyer
发明人： Laura Potter , Michael Nuccio , Rex Dwyer
IPC分类号： C40B10/00 , C40B60/00
CPC分类号： G16B20/00 , G16B25/00 , G16B35/00 , G16C20/60
摘要： Various systems and methods for selecting candidate biological components and/or combinations of biological components that affect a biological process are described. For example, a computing device may use a computer model to simulate the biological process and predict a phenotypic outcome. In this manner, the impact of candidate components and combinations may be determined using the computer model. The computing device may determine optimal characteristics such as expression levels of biological components that result in a desirable phenotypic outcome of the biological process as predicted by the computer model. The computing device may perform sensitivity analysis around the optimal characteristics. The sensitivity analysis may be used to determine whether the candidate combinations are robust across a range of the optimal characteristics. The computing device may select various candidate components and combinations based on the sensitivity analysis and the predicted phenotypic outcome.
摘要翻译：描述了用于选择候选生物组分和/或影响生物过程的生物组分的组合的各种系统和方法。例如，计算设备可以使用计算机模型来模拟生物过程并预测表型结果。以这种方式，可以使用计算机模型来确定候选组件和组合的影响。计算设备可以确定最佳特征，例如生物成分的表达水平，其导致如计算机模型预测的生物过程的期望的表型结果。计算设备可以围绕最佳特征执行灵敏度分析。灵敏度分析可以用于确定候选组合在最佳特征的范围内是否稳定。计算设备可以基于灵敏度分析和预测的表型结果来选择各种候选组分和组合。

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