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    • 5. 发明授权
    • Meconium assay procedure
    • 胎粪检测程序
    • US5587323A
    • 1996-12-24
    • US433232
    • 1995-05-02
    • Douglas E. LewisChristine M. Moore
    • Douglas E. LewisChristine M. Moore
    • G01N33/94G01N1/18
    • G01N33/94Y10S436/816Y10S436/901Y10T436/141111Y10T436/147777Y10T436/25Y10T436/25375Y10T436/255
    • A improved method for preparing a concentrated meconium extract containing basic and acid-neutral target analytes present in a meconium sample suspected of containing the target analytes is provided. The meconium sample is extracted with a mixed solvent of acetonitrile and a volatile organic acid and the resulting organic phase is separated. The organic phase is diluted with water to provide an organic solution which is passed through a mixed mode solid phase extraction column having strong cation exchange and hydrophobic functionality. Acid-neutral and basic target analytes are sequentially eluted from the column and the eluates are combined to form a combined extract. The combined extract is evaporated to dryness and reconstituted in buffer to provide a clean concentrated neonatal meconium extract for use in qualitative and quantitative analyses.
    • 提供了一种用于制备含有含有目标分析物的胎粪样品中存在的碱性和酸中性目标分析物的浓缩胎粪提取物的改进方法。 用乙腈和挥发性有机酸的混合溶剂萃取胎粪样品,分离得到的有机相。 有机相用水稀释以提供有机溶液,其通过具有强阳离子交换和疏水官能度的混合模式固相萃取柱。 酸性中性和碱性目标分析物从柱中顺序洗脱,并将洗脱液合并形成组合的提取物。 将合并的提取物蒸发至干,并在缓冲液中重构,以提供清洁的浓缩新生儿胎粪提取物,用于定性和定量分析。