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1. 发明申请

WO2018097844A1 METHODS, SYSTEMS, AND COMPOSITIONS FOR PROPAGATION OF A FERMENTATION MICROORGANISM 审中-公开
公开(公告)号：WO2018097844A1
公开(公告)日：2018-05-31
申请号：PCT/US2016/066486
申请日：2016-12-14
申请人： SHELL OIL COMPANY , SHELL INTERNATIONALE RESEARCH MAATSCHAPPIJ B.V.
发明人： ZUROFF, Trevor Roman , LIANG, Yi , BOTELLA-FRANCO, Carolina , PARKER, David Alexander , ZHANG, Kun
IPC分类号： C12P7/14 , C12P7/06 , C12P7/10 , C12N1/16 , C12N1/22
CPC分类号： C12P7/10 , C12N1/18 , C12N1/22 , C12N9/2437 , C12P7/14 , Y02E50/16 , Y02E50/17
摘要： Methods, systems, and compositions for propagating a fermentation microorganism that can have improved product yield during fermentation of a hydrolysate containing at least one growth inhibitor, including ethanol. In some embodiments, the method comprises: (a) providing a hydrolysate for fermentation, wherein the hydrolysate comprises exogenous ethanol in an amount of at least 10 g/L; (b) providing a propagation reactor containing: a propagation medium comprising: the hydrolysate in an amount of at least 25% by volume and glucose in an amount of greater than 14 g/L; and a first cell mass of a fermentation microorganism; and (c) propagating the first cell mass in the propagation medium for a time period to form a second cell mass of the fermentation microorganism that is greater than the first cell mass, where the hydrolysate contains less than 20% total solids (TS).

2. 发明申请

WO2020109268A1 PRODUCTION OF ETHANOL AND ENHANCED CO-PRODUCTS USING CO-PRODUCTS AS FEEDSTOCK 审中-公开
公开(公告)号：WO2020109268A1
公开(公告)日：2020-06-04
申请号：PCT/EP2019/082503
申请日：2019-11-26
申请人： SHELL INTERNATIONALE RESEARCH MAATSCHAPPIJ B.V. , SHELL OIL COMPANY
发明人： BOTELLA-FRANCO, Carolina , SADASIVAN VIJAYAKUMARI, Sivakumar , BLACKBOURN, Robert, Lawrence , WEIDER, Paul, Richard , CHEN, Ye-Mon , LIANG, Yi , GAGNE, Daniel , BAUGH, Ashley, Villarreal
IPC分类号： A23K10/38 , C12P7/08 , C12P7/10
摘要： Methods for production of ethanol, distiller's corn oil, and enhanced co-products are disclosed. Methods include obtaining a mixture of one or more co-products of an alcohol production process, which may include wet cake, performing hydrolysis of polysaccharides in the mixture to generate fermentable sugars, fermenting the fermentable sugars in the mixture to produce alcohol, distilling the mixture to remove alcohol from the mixture thereby producing alcohol-containing distillate and enhanced whole stillage, removing released oil from the fermented mixture before distillation and/or from the enhanced whole stillage after distillation, and recovering enhanced wet distiller's grains, enhanced thin stillage, and/or enhanced dried distiller's grains. Compositions disclosed herein include enhanced dried distiller's grains having a crude protein content of at least 45% on a dry weight basis and having a total fat content of less than 10% on a dry weight basis.

3. 发明申请

WO2017112475A1 METHODS, SYSTEMS, AND COMPOSITIONS FOR PROPAGATION OF A FERMENTATION MICROORGANISM 审中-公开
标题翻译：用于发酵微生物的方法，系统和组合物
公开(公告)号：WO2017112475A1
公开(公告)日：2017-06-29
申请号：PCT/US2016/066480
申请日：2016-12-14
申请人： SHELL OIL COMPANY , SHELL INTERNATIONALE RESEARCH MAATSCHAPPIJ B.V.
发明人： PARKER, David Alexander , ZHANG, Kun , BOTELLA-FRANCO, Carolina , LIANG, Yi , ZUROFF, Trevor Roman
IPC分类号： C12P7/06 , C12P7/08 , C12P7/10 , C12N1/22 , C12N1/32
CPC分类号： C12N1/22 , C12N1/18 , C12N1/32 , C12P7/10 , Y02E50/16
摘要： Methods, systems, and compositions for propagating a fermentation microorganism that can have improved product yield during fermentation of a hydrolysate containing at least one growth inhibitor, including ethanol. In some embodiments, the method comprises: (a) providing a hydrolysate for fermentation, wherein the hydrolysate comprises exogenous ethanol in an amount of at least 10 g/L; (b) providing a propagation reactor containing: a propagation medium comprising: the hydrolysate in an amount of at least 25% by volume and glucose in an amount of greater than 14 g/L; and a first cell mass of a fermentation microorganism; and (c) propagating the first cell mass in the propagation medium for a time period to form a second cell mass of the fermentation microorganism that is greater than the first cell mass.
摘要翻译：用于繁殖发酵微生物的方法，系统和组合物，所述发酵微生物在含有至少一种生长抑制剂（包括乙醇）的水解产物的发酵期间可具有提高的产物产量。在一些实施方案中，所述方法包括：（a）提供用于发酵的水解产物，其中所述水解产物包含至少10g / L量的外源乙醇; （b）提供增殖反应器，所述增殖反应器包含：繁殖介质，其包含：至少25体积％的量的水解产物和大于14g / L的量的葡萄糖; 和发酵微生物的第一细胞块; 和（c）使第一细胞团在繁殖培养基中繁殖一段时间，以形成比第一细胞团大的发酵微生物的第二细胞团。

4. 发明申请

WO2017112471A1 METHODS, SYSTEMS, AND COMPOSITIONS FOR PROPAGATION OF A FERMENTATION MICROORGANISM 审中-公开
标题翻译：用于发酵微生物的方法，系统和组合物
公开(公告)号：WO2017112471A1
公开(公告)日：2017-06-29
申请号：PCT/US2016/066469
申请日：2016-12-14
申请人： SHELL OIL COMPANY , SHELL INTERNATIONALE RESEARCH MAATSCHAPPIJ B.V.
发明人： PARKER, David Alexander , ZHANG, Kun , BOTELLA-FRANCO, Carolina , LIANG, Yi , ZUROFF, Trevor Roman
IPC分类号： C12N1/18 , C12N1/32 , C12N1/16
CPC分类号： C12N1/16 , C12N1/18 , C12N1/32
摘要： Methods, systems, and compositions for propagating a fermentation microorganism that can have improved product yield during fermentation of a hydrolysate containing at least one growth inhibitor, including ethanol. In some embodiments, the method comprises (a) providing a generated according to a method comprising: - ensiling a cellulosic biomass material for at least 24 hours to generate ethanol in the cellulosic biomass material to generate an ensiled cellulosic biomass material; - pretreating the ensiled cellulosic biomass material with a solution comprising an alpha-hydroxysulfonic acid to produce a pretreated material comprising ethanol; and - introducing one or more cellulases to the pretreated material to produce the hydrolysate; (b) providing a propagation reactor containing: a propagation medium comprising: the hydrolysate in an amount of at least 25% by volume and glucose in an amount of greater than 14 g/L; and a first cell mass of a fermentation microorganism.
摘要翻译：用于繁殖发酵微生物的方法，系统和组合物，所述发酵微生物在含有至少一种生长抑制剂（包括乙醇）的水解产物的发酵期间可具有提高的产物产量。在一些实施方案中，所述方法包括（a）提供根据以下方法产生的方法，所述方法包括： - 将纤维素类生物质材料保温至少24小时以在纤维素类生物质材料中产生乙醇以产生青贮的纤维素类生物质材料; - 用包含α-羟基磺酸的溶液预处理所述青贮的纤维素类生物质材料以产生包含乙醇的预处理材料; 和 - 将一种或多种纤维素酶引入预处理的材料以产生水解产物; （b）提供增殖反应器，所述增殖反应器包含：繁殖介质，其包含：至少25体积％的量的水解产物和大于14g / L的量的葡萄糖; 和发酵微生物的第一细胞团。

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