专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US08927703B2 Compositions, kits and related methods for the detection and/or monitoring of Pseudomonas aeruginosa 有权
标题翻译：用于检测和/或监测铜绿假单胞菌的组合物，试剂盒和相关方法
公开(公告)号：US08927703B2
公开(公告)日：2015-01-06
申请号：US12061438
申请日：2008-04-02
申请人： Shannon K. Kaplan , Kristin Livezey , Jennifer J. Bungo , James J. Hogan
发明人： Shannon K. Kaplan , Kristin Livezey , Jennifer J. Bungo , James J. Hogan
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/689
摘要： The present invention provides compositions, methods and kits for the species-specific detection of Pseudomonas aeruginosa.
摘要翻译：本发明提供了用于铜绿假单胞菌物种特异性检测的组合物，方法和试剂盒。

2. 发明申请

US20080268452A1 COMPOSITIONS, KITS AND RELATED METHODS FOR THE DETECTION AND/OR MONITORING OF PSEUDOMONAS AERUGINOSA 有权
标题翻译：用于检测和/或监测PSEUDOMONAS AERUGINOSA的组合物，试剂盒和相关方法
公开(公告)号：US20080268452A1
公开(公告)日：2008-10-30
申请号：US12061438
申请日：2008-04-02
申请人： Shannon K. Kaplan , Kristin Livezey , Jennifer J. Bungo , James J. Hogan
发明人： Shannon K. Kaplan , Kristin Livezey , Jennifer J. Bungo , James J. Hogan
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689
摘要： The present invention provides compositions, methods and kits for the species-specific detection of pseudomonas aeruginosa.
摘要翻译：本发明提供了用于种类特异性检测铜绿假单胞菌的组合物，方法和试剂盒。

3. 发明申请

US20070202523A1 METHODS AND KITS FOR AMPLIFYING DNA 有权
标题翻译：用于扩增DNA的方法和工具
公开(公告)号：US20070202523A1
公开(公告)日：2007-08-30
申请号：US11681104
申请日：2007-03-01
申请人： Michael BECKER , Wai-Chung Lam , Kristin Livezey , Steven Brentano , Daniel Kolk , Astrid Schroder
发明人： Michael BECKER , Wai-Chung Lam , Kristin Livezey , Steven Brentano , Daniel Kolk , Astrid Schroder
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12P19/34 , C12Q1/6865 , C12Q2533/101 , C12Q2525/186 , C12Q2525/143 , C12Q2537/163 , C12Q2521/325 , C12Q2521/107
摘要： Novel methods of synthesizing multiple copies of a target nucleic acid sequence which are autocatalytic are disclosed (i.e., able to cycle automatically without the need to modify reaction conditions such as temperature, pH, or ionic strength and using the product of one cycle in the next one). In particular, methods of nucleic acid amplification are disclosed which are robust and efficient, while reducing the appearance of side-products. In general, the methods use priming oligonucleotides that target only one sense of a target nucleic acid, a promoter oligonucleotide modified to prevent polymerase extension from its 3′-terminus and, optionally, a means for terminating a primer extension reaction, to amplify RNA or DNA molecules in vitro, while reducing or substantially eliminating the formation of side-pro ducts. The disclosed methods minimizes or substantially eliminate the emergence of side-products, thus providing a high level of specificity. Furthermore, the appearance of side-products can complicate the analysis of the amplification reaction by various molecular detection techniques. The disclosed methods minimize or substantially eliminate this problem, thus providing enhanced levels of sensitivity.
摘要翻译：公开了合成自动催化的靶核酸序列的多个拷贝的新方法（即，能够自动循环而不需要修改反应条件如温度，pH或离子强度，并且使用下一个循环的产物一）。特别地，公开了鲁棒和有效的核酸扩增方法，同时减少副产物的外观。通常，所述方法使用仅靶向靶核酸的引物寡核苷酸，经修饰以防止聚合酶从其3'末端延伸的启动子寡核苷酸，以及任选的终止引物延伸反应的手段以扩增RNA或 DNA分子在体外，同时减少或基本上消除副产物的形成。所公开的方法最小化或基本上消除副产物的出现，从而提供高水平的特异性。此外，副产物的出现可能通过各种分子检测技术使扩增反应的分析复杂化。所公开的方法最小化或基本上消除了该问题，从而提供了增强的灵敏度水平。

4. 发明申请

US20070054301A1 Methods, compositions and kits for isothermal amplification of nucleic acids 审中-公开
标题翻译：用于核酸等温扩增的方法，组合物和试剂盒
公开(公告)号：US20070054301A1
公开(公告)日：2007-03-08
申请号：US11517117
申请日：2006-09-06
申请人： Michael Becker , Kristin Livezey
发明人： Michael Becker , Kristin Livezey
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6844 , C12P19/34 , C12Q1/6853 , C12Q2527/101 , C12Q2537/155 , C12Q2525/161 , C12Q2525/179 , C12Q2537/1376
摘要： Methods and compositions are described for isothermal nucleic acid amplification of a nucleic acid template strand by using an oligonucleotide primer that includes an AT-rich nucleotide sequence and a polymerase having strand displacement activity.
摘要翻译：描述了通过使用包含富含AT的核苷酸序列的寡核苷酸引物和具有链置换活性的聚合酶的核酸模板链的等温核酸扩增的方法和组合物。

5. 发明申请

US20070299254A1 Kits for Performing Amplification Reactions 有权
标题翻译：用于进行放大反应的试剂盒
公开(公告)号：US20070299254A1
公开(公告)日：2007-12-27
申请号：US11574307
申请日：2005-08-26
申请人： Michael Becker , Wal-Chung Lam , Kristin Livezey , Steven Brentano , Daniel Kolk , Astrid Schroder
发明人： Michael Becker , Wal-Chung Lam , Kristin Livezey , Steven Brentano , Daniel Kolk , Astrid Schroder
IPC分类号： C07H21/04
CPC分类号： C12P19/34 , C12Q1/6865 , C12Q2537/163 , C12Q2521/325 , C12Q2521/107 , C12Q2525/186 , C12Q2533/101 , C12Q2525/143
摘要： The present invention is directed to novel methods of synthesizing multiple copies of a target nucleic acid sequence which are autocatalytic (i.e., able to cycle automatically without the need to modify reaction conditions such as temperature, pH, or ionic strength and using the product of one cycle in the next one). In particular, the present invention discloses a method of nucleic acid amplification which is robust and efficient, while reducing the appearance of side products. The method uses only one primer, the “priming oligonucleotide,” a 3'blocked promoter oligonucleotide and optionally, a means for terminating a primer extension reaction, to amplify RNA or DNA molecules in vitro, while reducing or eliminating the formation of side products. The method of the present invention minimizes or eliminates the emergence of side products, thus providing a high level of specificity. Furthermore, the appearance of side products can complicate the analysis of the amplification reaction by various molecular detection techniques. The present invention minimizes or eliminates this problem, thus providing an enhanced level of sensitivity.
摘要翻译：本发明涉及合成目标核酸序列的多个拷贝的新型方法，所述目标核酸序列是自动催化的（即，能够自动循环而不需要修饰诸如温度，pH或离子强度的反应条件，并且使用一种循环在下一个）。特别地，本发明公开了一种鲁棒有效的核酸扩增方法，同时减少了副产物的外观。该方法仅使用一种引物，即引物寡核苷酸，3'封闭的启动子寡核苷酸和任选的终止引物延伸反应的手段，以体外扩增RNA或DNA分子，同时减少或消除副产物的形成。本发明的方法使副产物的出现最小化或消除，从而提供高水平的特异性。此外，副产物的出现可能通过各种分子检测技术使扩增反应的分析复杂化。本发明使这个问题最小化或消除了这一点，从而提供了增强的灵敏度。

6. 发明申请

US20060046265A1 Single-primer nucleic acid amplification methods 有权
标题翻译：单引物核酸扩增方法
公开(公告)号：US20060046265A1
公开(公告)日：2006-03-02
申请号：US11213519
申请日：2005-08-26
申请人： Michael Becker , Wai-Chung Lam , Kristin Livezey , Steven Brentano , Daniel Kolk , Astrid Schroder
发明人： Michael Becker , Wai-Chung Lam , Kristin Livezey , Steven Brentano , Daniel Kolk , Astrid Schroder
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12P19/34 , C12Q1/6865 , C12Q2537/163 , C12Q2521/325 , C12Q2521/107 , C12Q2525/186 , C12Q2533/101 , C12Q2525/143
摘要： The present invention is directed to novel methods of synthesizing multiple copies of a target nucleic acid sequence which are autocatalytic (i.e., able to cycle automatically without the need to modify reaction conditions such as temperature, pH, or ionic strength and using the product of one cycle in the next one). In particular, the present invention discloses a method of nucleic acid amplification which is robust and efficient, while reducing the appearance of side-products. The method uses only one primer, the “priming oligonucleotide,” a promoter oligonucleotide modified to prevent polymerase extension from its 3′-terminus and, optionally, a means for terminating a primer extension reaction, to amplify RNA or DNA molecules in vitro, while reducing or substantially eliminating the formation of side-products. The method of the present invention minimizes or substantially eliminates the emergence of side-products, thus providing a high level of specificity. Furthermore, the appearance of side-products can complicate the analysis of the amplification reaction by various molecular detection techniques. The present invention minimizes or substantially eliminates this problem, thus providing an enhanced level of sensitivity.
摘要翻译：本发明涉及合成目标核酸序列的多个拷贝的新型方法，所述目标核酸序列是自动催化的（即，能够自动循环而不需要修饰诸如温度，pH或离子强度的反应条件，并且使用一种循环在下一个）。特别地，本发明公开了一种鲁棒有效的核酸扩增方法，同时减少副产物的外观。该方法仅使用一种引物，即引物寡核苷酸，经修饰的启动子寡核苷酸，用于防止聚合酶从其3'末端延伸，以及任选的终止引物延伸反应的手段，以在体外扩增RNA或DNA分子，同时减少或基本上消除副产物的形成。本发明的方法使副产物的出现最小化或基本上消除，从而提供高水平的特异性。此外，副产物的出现可能通过各种分子检测技术使扩增反应的分析复杂化。本发明使这个问题最小化或基本消除，从而提供了增强的灵敏度。

7. 发明申请

US20070281317A1 Tagged oligonucleotides and their use in nucleic acid amplification methods 有权
标题翻译：标记的寡核苷酸及其在核酸扩增方法中的应用
公开(公告)号：US20070281317A1
公开(公告)日：2007-12-06
申请号：US11810834
申请日：2007-06-06
申请人： Michael Becker , Kristin Livezey , Wai-Chung Lam
发明人： Michael Becker , Kristin Livezey , Wai-Chung Lam
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6848 , C12N15/1065 , C12Q1/6806 , C12Q1/689 , C12Q1/6895 , C12Q2525/155
摘要： The present invention provides nucleic acid amplification methods that desirably reduce or eliminate false positive amplification signals resulting from contaminating biological material, e.g., nucleic acid, that may be present in one or more reagents used in an amplification reaction and/or that may be present in the environment in which an amplification reaction is performed. The invention offers the further advantage of requiring less stringent purification and/or sterility efforts than conventionally needed in order to ensure that enzymes and other reagents used in amplification reactions, and the environment in which an amplification reaction is performed, are free of bacterial or other nucleic acid contamination that may yield false positive results.
摘要翻译：本发明提供了核酸扩增方法，其期望地减少或消除由污染生物材料（例如核酸）产生的假阳性扩增信号，所述核酸可以存在于用于扩增反应的一种或多种试剂中和/或可存在于进行扩增反应的环境。本发明提供了进一步的优点，即需要比常规所需的更少的纯化和/或不育努力，以确保用于扩增反应的酶和其它试剂以及进行扩增反应的环境不含细菌或其它可能产生假阳性结果的核酸污染物。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式