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1. 发明申请

US20130040366A1 METAGENOME-DERIVED ALKALINE PHOSPHATASE 有权
标题翻译： METAGENOME衍生的碱性磷酸酶
公开(公告)号：US20130040366A1
公开(公告)日：2013-02-14
申请号：US13519013
申请日：2010-06-08
申请人： Seung Goo Lee , Su Lim Choi , Eugene Rha , Jae Jun Song
发明人： Seung Goo Lee , Su Lim Choi , Eugene Rha , Jae Jun Song
IPC分类号： C12N9/16 , C12N1/15 , C12N1/21 , C12N1/19 , C12N15/55 , C12N15/63
CPC分类号： C12N9/16
摘要： The present invention relates to a metagenome-derived alkaline phosphatase, and more particularly to a novel, metagenome-derived alkaline phosphatase screened using a artificial genetic circuit that detects phenolic compounds, and a preparation method thereof. A novel alkaline phosphatase according to the present invention has high activity of dephosphorylating DNA and can be inactivated rapidly by simple heat treatment. Thus, it can be used for a dephosphorylation reaction so that genetic manipulations, including genetic cloning, become efficient. In addition, it can be actively expressed in recombinant microorganisms, and thus can be used in various assays, including enzyme immunoassay.
摘要翻译：本发明涉及一种来源于碱金属蛋白酶的碱性磷酸酶，更具体地说，涉及使用检测酚类化合物的人工遗传回路筛选的新颖的，基因突变型的碱性磷酸酶及其制备方法。根据本发明的新型碱性磷酸酶具有高的去磷酸化DNA的活性，并且可以通过简单的热处理快速灭活。因此，它可以用于去磷酸化反应，使得遗传操作（包括遗传克隆）变得有效。此外，它可以在重组微生物中有效表达，因此可用于各种测定，包括酶免疫测定。

2. 发明申请

US20120238470A1 METHOD FOR SCREENING AND QUANTIFYING VARIOUS ENZYME ACTIVITIES USING A GENETIC ENZYME SCREENING SYSTEM 有权
标题翻译：使用遗传酶筛选系统筛选和定量各种酶活性的方法
公开(公告)号：US20120238470A1
公开(公告)日：2012-09-20
申请号：US13376783
申请日：2010-06-08
申请人： Seung Goo Lee , Eugene Rha , Su Lim Choi , Jae Jun Song , Jong Hyun Choi , Hee Sik Kim
发明人： Seung Goo Lee , Eugene Rha , Su Lim Choi , Jae Jun Song , Jong Hyun Choi , Hee Sik Kim
IPC分类号： C40B30/08
CPC分类号： C12Q1/6897 , C12N15/1086
摘要： A method of detecting and quantifying various enzymatic activities using a constructed artificial genetic circuit GESS (genetic enzyme screening system) for sensing phenolic compounds and a method of screening a trace of activities of target enzymes from a metagenome using the artificial genetic circuit, thereby securing target enzyme genes. When the method for screening and quantifying target enzymatic activity is used, useful genes can be screened from various genetic communities, including environmental or metagenomic libraries, at a single cell level in high throughput (million/day). Further, the sensitivity of the genetic circuit to phenol derivatives and the expression thereof can be controlled, and thus the genetic circuit can rapidly sense and quantify various enzymatic activities. Thus, the method can be advantageously used in the protein engineering technology for enzyme modification. Particularly, it can quantitatively investigate enzymatic activity, and thus can be applied to molecular evolution technology.
摘要翻译：使用用于感测酚类化合物的构建的人工遗传回路GESS（遗传酶筛选系统）检测和定量各种酶活性的方法，以及使用人造遗传回路从宏基因组筛选靶酶的活性痕迹的方法，由此确保靶酶基因。当使用筛选和定量靶酶活性的方法时，可以在高通量（百万/天）的单个细胞水平上从各种遗传群落（包括环境或宏基因组文库）筛选有用的基因。此外，可以控制遗传回路对苯酚衍生物的敏感性及其表达，因此遗传回路可以快速感测和定量各种酶活性。因此，该方法可有利地用于酶修饰的蛋白质工程技术中。特别是可以定量研究酶活性，从而可以应用于分子进化技术。

3. 发明授权

US09783859B2 Method of screening and quantifying various enzymatic activities using artificial genetic circuits 有权
公开(公告)号：US09783859B2
公开(公告)日：2017-10-10
申请号：US13376783
申请日：2010-06-08
申请人： Seung Goo Lee , Eugene Rha , Su Lim Choi , Jae Jun Song , Jong Hyun Choi , Hee Sik Kim
发明人： Seung Goo Lee , Eugene Rha , Su Lim Choi , Jae Jun Song , Jong Hyun Choi , Hee Sik Kim
IPC分类号： C40B30/00 , C12Q1/68 , C12N15/10
CPC分类号： C12Q1/6897 , C12N15/1086
摘要： A method of detecting and quantifying various enzymatic activities using a constructed artificial genetic circuit GESS (genetic enzyme screening system) for sensing phenolic compounds and a method of screening a trace of activities of target enzymes from a metagenome using the artificial genetic circuit, thereby securing target enzyme genes. When the method for screening and quantifying target enzymatic activity is used, useful genes can be screened from various genetic communities, including environmental or metagenomic libraries, at a single cell level in high throughput (million/day). Further, the sensitivity of the genetic circuit to phenol derivatives and the expression thereof can be controlled, and thus the genetic circuit can rapidly sense and quantify various enzymatic activities. Thus, the method can be advantageously used in the protein engineering technology for enzyme modification. Particularly, it can quantitatively investigate enzymatic activity, and thus can be applied to molecular evolution technology.

4. 发明授权

US08647854B2 Metagenome-derived alkaline phosphatase 有权
标题翻译：来自Metagenome的碱性磷酸酶
公开(公告)号：US08647854B2
公开(公告)日：2014-02-11
申请号：US13519013
申请日：2010-06-08
申请人： Seung Goo Lee , Su Lim Choi , Eugene Rha , Jae Jun Song
发明人： Seung Goo Lee , Su Lim Choi , Eugene Rha , Jae Jun Song
IPC分类号： C12N9/16
CPC分类号： C12N9/16
摘要： The present invention relates to a metagenome-derived alkaline phosphatase, and more particularly to a novel, metagenome-derived alkaline phosphatase screened using a artificial genetic circuit that detects phenolic compounds, and a preparation method thereof. A novel alkaline phosphatase according to the present invention has high activity of dephosphorylating DNA and can be inactivated rapidly by simple heat treatment. Thus, it can be used for a dephosphorylation reaction so that genetic manipulations, including genetic cloning, become efficient. In addition, it can be actively expressed in recombinant microorganisms, and thus can be used in various assays, including enzyme immunoassay.
摘要翻译：本发明涉及一种来源于碱金属蛋白酶的碱性磷酸酶，更具体地说，涉及使用检测酚类化合物的人工遗传回路筛选的新颖的，基因突变型的碱性磷酸酶及其制备方法。根据本发明的新型碱性磷酸酶具有高的去磷酸化DNA的活性，并且可以通过简单的热处理快速灭活。因此，它可以用于去磷酸化反应，使得遗传操作（包括遗传克隆）变得有效。此外，它可以在重组微生物中有效表达，因此可用于各种测定，包括酶免疫测定。

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