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1. 发明授权

US10077468B2 PCR primer capable of reducing non-specific amplification and PCR method using the PCR primer 有权
公开(公告)号：US10077468B2
公开(公告)日：2018-09-18
申请号：US12228302
申请日：2008-08-12
申请人： Seongjun Yoon , Hyunyoung Lee , Jinseok Yu , Sooyoun Jun , Sanghyeon Kang
发明人： Seongjun Yoon , Hyunyoung Lee , Jinseok Yu , Sooyoun Jun , Sanghyeon Kang
IPC分类号： C12Q1/68 , C12Q1/686
CPC分类号： C12Q1/686 , C12Q2549/101 , C12Q2525/151
摘要： The present invention relates to a PCR primer facilitating hot-start PCR by suppressing non-specific amplification at room temperature and at the same time capable of reducing significantly non-specific amplification by dominating the amplification of the PCR product rather than the amplification of the original template from the third PCR cycle, more precisely a PCR primer prepared by additionally inserting the reverse-complementary sequence to a certain region starting from the 5′-start site of the 5′-terminus of the original primer which is composed of priming sequence to anneal to a PCR template into the 5′-terminus of the original primer and a PCR method using the same. The primer of the present invention has a original primer sequence composed of priming sequence to anneal to a PCR template and an additional reverse-complementary sequence, which inserted into the 5′-terminus of the original primer, to a certain region starting from the 5′-start site of the 5′-terminus of the original primer sequence, suggesting that a template-specific sequence and its reverse-complementary sequence are included in the same primer. The present invention can improve PCR specificity by reducing non-specific amplification.

2. 发明申请

US20090258353A1 PCR primer capable of reducing non-specific amplification and PCR method using the PCR primer 审中-公开
标题翻译：使用PCR引物能够减少非特异性扩增和PCR方法的PCR引物
公开(公告)号：US20090258353A1
公开(公告)日：2009-10-15
申请号：US12228302
申请日：2008-08-12
申请人： Seongjun Yoon , Hyunyoung Lee , Jinseok Yu , Sooyoun Jun , Sanghyeon Kang
发明人： Seongjun Yoon , Hyunyoung Lee , Jinseok Yu , Sooyoun Jun , Sanghyeon Kang
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/686 , C12Q2549/101 , C12Q2525/151
摘要： The present invention relates to a PCR primer facilitating hot-start PCR by suppressing non-specific amplification at room temperature and at the same time capable of reducing significantly non-specific amplification by dominating the amplification of the PCR product rather than the amplification of the original template from the third PCR cycle, more precisely a PCR primer prepared by additionally inserting the reverse-complementary sequence to a certain region starting from the 5′-start site of the 5′-terminus of the original primer which is composed of priming sequence to anneal to a PCR template into the 5′-terminus of the original primer and a PCR method using the same. The primer of the present invention has a original primer sequence composed of priming sequence to anneal to a PCR template and an additional reverse-complementary sequence, which inserted into the 5′-terminus of the original primer, to a certain region starting from the 5′-start site of the 5′-terminus of the original primer sequence, suggesting that a template-specific sequence and its reverse-complementary sequence are included in the same primer. The present invention can improve PCR specificity by reducing non-specific amplification.
摘要翻译：本发明涉及通过在室温下抑制非特异性扩增而促进热启动PCR的PCR引物，并且同时能够通过主导PCR产物的扩增来显着降低非特异性扩增，而不是原始扩增来自第三PCR循环的模板，更确切地说，是通过将原始引物的5'末端的5'-起始位点开始的反向互补序列另外插入到由引发序列组成的特定区域而制备的PCR引物，与PCR模板退火到原始引物的5'末端，使用PCR法进行PCR扩增。本发明的引物具有由引物序列组成的原始引物序列，其与PCR模板退火，并将插入原始引物的5'末端的另外的反向互补序列从5 '起始引物序列的5'末端的位点，表明模板特异性序列及其反向互补序列被包含在相同的引物中。本发明可以通过减少非特异性扩增来提高PCR特异性。

3. 发明授权

US08043613B2 Podoviriedae bacteriophage having killing activity specific to Staphylococcus aureus 有权
标题翻译：波多病毒噬菌体具有对金黄色葡萄球菌特异性的杀伤活性
公开(公告)号：US08043613B2
公开(公告)日：2011-10-25
申请号：US12378457
申请日：2009-02-12
申请人： Seongjun Yoon , Yunjaie Choi , Seyung Lee , Jeesoo Son , Sooyoun Jun , Sanghyeon Kang
发明人： Seongjun Yoon , Yunjaie Choi , Seyung Lee , Jeesoo Son , Sooyoun Jun , Sanghyeon Kang
IPC分类号： A01N63/00
CPC分类号： A61K35/76 , C12N7/00 , C12N2795/10232
摘要： The present invention relates to a novel bacteriophage, more precisely a Podoviridae bacteriophage having killing activity specific to Staphylococcus aureus which is the causing agent of infectious disease in human and animals, a pharmaceutical composition for the prevention and treatment of the disease caused by Staphylococcus aureus, an antibiotic and a disinfectant containing the bacteriophage as an active ingredient.
摘要翻译：本发明涉及一种新型噬菌体，更确切地说，具有针对人和动物感染性疾病引起的金黄色葡萄球菌特异性杀伤活性的波形病毒噬菌体，用于预防和治疗由金黄色葡萄球菌引起的疾病的药物组合物，含有噬菌体作为活性成分的抗生素和消毒剂。

4. 发明申请

US20100203180A1 Antimicrobial protein derived from podoviriedae bacteriophage specific to staphylococcus aureus 有权
标题翻译：源自金黄色葡萄球菌特异性的巨球菌噬菌体的抗菌蛋白质
公开(公告)号：US20100203180A1
公开(公告)日：2010-08-12
申请号：US12378365
申请日：2009-02-12
申请人： Seongjun Yoon , Yunjaie Choi , Seyung Lee , Jeesoo Son , Sooyoun Jun , Sanghyeon Kang
发明人： Seongjun Yoon , Yunjaie Choi , Seyung Lee , Jeesoo Son , Sooyoun Jun , Sanghyeon Kang
IPC分类号： A61K38/16 , C07K14/01 , C12N15/31 , C12N1/21 , A61P31/04
CPC分类号： C07K14/005 , A61K9/0017 , A61K38/00 , C12N9/503 , C12N2795/10222
摘要： The present invention relates to a novel antimicrobial protein derived from bacteriophage having killing activity specific to Staphylococcus aureus, more precisely an antimicrobial protein originated from Podoviridae bacteriophage having killing activity specific to Staphylococcus aureus which is the causing agent of infectious disease in human and animals, a pharmaceutical composition for the prevention and treatment of infectious disease caused by Staphylococcus aureus, an antibiotic and a disinfectant containing the bacteriophage-originated antimicrobial protein as an active ingredient.
摘要翻译：本发明涉及一种新型的抗菌药物，其具有对金黄色葡萄球菌特异性杀伤活性的噬菌体，更准确地说，是源于波氏病毒噬菌体的抗微生物蛋白质，其具有对人和动物感染性疾病引起的金黄色葡萄球菌特异性杀伤活性，用于预防和治疗由金黄色葡萄球菌引起的感染性疾病的药物组合物，抗生素和含有噬菌体起始的抗微生物蛋白作为活性成分的消毒剂。

5. 发明申请

US20110014714A1 METHOD FOR SELECTIVE LABELING OF PROTEIN PRODUCED BY IN VITRO TRANSLATION BY USING A MARKER FROM TRNA PREPARED BY USING IN VITRO TRANSCRIPTION 有权
标题翻译：通过使用通过使用体外转录制备的TRNA的标记通过体外翻译产生的蛋白质的选择性标记的方法
公开(公告)号：US20110014714A1
公开(公告)日：2011-01-20
申请号：US12812382
申请日：2009-01-07
申请人： Seongjun Yoon , SooYoun Jun , SangHyeon Kang
发明人： Seongjun Yoon , SooYoun Jun , SangHyeon Kang
IPC分类号： G01N33/68 , C12P19/34 , C07H21/02
CPC分类号： G01N33/533 , G01N33/532
摘要： The present invention relates to a method for the preparation of a labeling material (labeling agent) usable for non-radioactive selective labeling of proteins produced by in vitro translation with fluorophore or biotin; and a method for selective labeling of proteins produced by in vitro translation using the said labeling material. More specifically, the present invention provides a method for preparing a labeling material usable for selective labeling of a target protein produced only from the gene added to the cell-free protein synthesis reaction mixture by an experimenter without labeling pre-existing proteins in the reaction mixture, for which only one type of tRNA prepared by in vitro transcription is used instead of “total tRNA mixture”. The present invention also provides a method for labeling of the protein produced by in vitro translation by using the labeling material prepared by the above method. According to the present invention, the preparation of a labeling material becomes easier and the protein labeled with the labeling material of the present invention provides much improved signals than the protein labeled with the labeling material of the conventional method prepared by using “total tRNA mixture” as a tRNA material.
摘要翻译：本发明涉及制备可用于通过用荧光团或生物素的体外翻译产生的蛋白质的非放射性选择性标记的标记材料（标记试剂）的方法; 以及使用所述标记材料通过体外翻译产生的蛋白质选择性标记的方法。更具体地说，本发明提供了一种可用于选择性标记仅通过实验者从无基因蛋白质合成反应混合物中添加的基因而产生的靶蛋白的标记物质的方法，而不在反应混合物中标记预先存在的蛋白质，其中仅使用通过体外转录制备的一种类型的tRNA代替“总tRNA混合物”。本发明还提供了通过使用通过上述方法制备的标签材料通过体外翻译产生的蛋白质的标记方法。根据本发明，标记材料的制备变得更容易，并且用本发明的标记材料标记的蛋白提供比使用通过使用“总tRNA混合物”制备的常规方法的标记材料标记的蛋白质更大的改善的信号，作为tRNA材料。

6. 发明申请

US20130011369A1 Method for Prevention and Treatment of Salmonella Infection 审中-公开
标题翻译：预防和治疗沙门氏菌感染的方法
公开(公告)号：US20130011369A1
公开(公告)日：2013-01-10
申请号：US13176458
申请日：2011-07-05
申请人： Seongjun Yoon , Sooyoun Jun , Hyoungrok Paik , Gimo Jung , Yoonsik Shin , Sanghyeon Kang
发明人： Seongjun Yoon , Sooyoun Jun , Hyoungrok Paik , Gimo Jung , Yoonsik Shin , Sanghyeon Kang
IPC分类号： A61K35/76 , C12N7/00 , A61P31/04
CPC分类号： C12N7/00 , A61K35/76 , C12N2795/00021 , C12N2795/00032 , Y02A50/471 , Y02A50/481
摘要： The present invention relates to a composition comprising bacteriophage SP-1, the bacteriophage capable of destroying Salmonella once being infected in Salmonella, as an active ingredient, and a method for prevention and treatment of Salmonella infection using the same. Bacteriophage SP-1, the active ingredient of the composition of the present invention, characteristically has the killing activity to Salmonella and has the genome represented by SEQ. ID. NO: 1.
摘要翻译：本发明涉及包含噬菌体SP-1，能够在沙门氏菌中被感染的沙门氏菌的噬菌体作为活性成分的组合物，以及使用该组合物的沙门氏菌感染的预防和治疗方法。噬菌体SP-1是本发明组合物的活性成分，其特征在于具有沙门氏菌的杀伤活性，并具有SEQ ID NO： ID。 NO：1。

7. 发明授权

US08066990B2 Lysin protein having broad antibacterial activity specific to bacteria 有权
标题翻译：赖氨酸蛋白具有对细菌特异性的广泛抗菌活性
公开(公告)号：US08066990B2
公开(公告)日：2011-11-29
申请号：US12423322
申请日：2009-04-14
申请人： Seongjun Yoon , Yunjaie Choi , Jeesoo Son , Sooyoun Jun , Hyoungrok Paik , Sanghyeon Kang
发明人： Seongjun Yoon , Yunjaie Choi , Jeesoo Son , Sooyoun Jun , Hyoungrok Paik , Sanghyeon Kang
IPC分类号： A61K38/46
CPC分类号： C12N9/503 , A61K38/00 , C12N2795/00011 , C12N2795/00022
摘要： The present invention relates to a lysin protein originated from bacteriophage, more precisely a lysin protein comprising the amino acid sequence represented by SEQ. ID. NO: 2 which has no harm to human and animals comprising eukaryotic cells owing to its specificity to bacteria and has broad antibacterial activity, and a pharmaceutical composition for the prevention and treatment of infectious disease caused by bacteria comprising the said lysin protein as an active ingredient.
摘要翻译：本发明涉及来自噬菌体的赖氨酸蛋白质，更确切地说，涉及包含SEQ ID NO：1所示氨基酸序列的赖氨酸蛋白质。 ID。 NO：2，由于其对细菌的特异性而对包含真核细胞的人类和动物无害，并且具有广泛的抗菌活性，以及用于预防和治疗由细菌引起的感染性疾病的药物组合物，所述细菌包含所述溶素蛋白作为活性成分。

8. 发明申请

US20100254950A1 Bacteriophage or lytic protein derived from the bacteriophage which effective for the treatment of staphylococcus aureus biofilm 有权
标题翻译：来自有效治疗金黄色葡萄球菌生物膜的噬菌体的噬菌体或溶解蛋白
公开(公告)号：US20100254950A1
公开(公告)日：2010-10-07
申请号：US12677990
申请日：2008-09-12
申请人： Seongjun Yoon , Yunjaie Choi , Se Yung Lee , Jeesoo Son , Sooyoun Jun , Sanghyeon Kang
发明人： Seongjun Yoon , Yunjaie Choi , Se Yung Lee , Jeesoo Son , Sooyoun Jun , Sanghyeon Kang
IPC分类号： A01N63/02 , A01N63/00 , A01P1/00 , A61K35/76 , A61K35/00 , A61P31/04
CPC分类号： A01N63/00 , A61K35/76 , A61K38/00 , C12N2795/00031 , C12N2795/00032
摘要： The present invention relates to compositions for removing a biofilm formed by Staphylococcus aureus, comprising a bacteriophage, such as Myoviridae family T4-like phage genus bacteriophage (Accession No: KCTC 11153BP, SAP-I) or Podoviridae family φ29-like virus genus bacteriophage (Accession No: KCTC11154BP, SAP-2), and lytic protein derived therefrom, that destroys the biofilm. Also disclosed are pharmaceutical compositions for the treatment of diseases caused by Staphylococcus aureus capable of forming biofilm.
摘要翻译：本发明涉及用于除去由金黄色葡萄球菌形成的生物膜的组合物，其包含噬菌体，例如Myoviridae家族T4样噬菌体噬菌体（登录号：KCTC 11153BP，SAP-1）或Podoviridae家族和类肿瘤病毒属噬菌体（登录号：KCTC11154BP，SAP-2）和从其衍生的裂解蛋白，破坏生物膜。还公开了用于治疗能够形成生物膜的由金黄色葡萄球菌引起的疾病的药物组合物。

9. 发明授权

US09121023B2 Polymerase chain reaction product-cloning vector suitable to its easy production and method for producing the same 有权
标题翻译：聚合酶链反应产物克隆载体适合其易于生产及其生产方法
公开(公告)号：US09121023B2
公开(公告)日：2015-09-01
申请号：US12740003
申请日：2008-10-29
申请人： Seongjun Yoon , SooYoun Jun , SangHyeon Kang
发明人： Seongjun Yoon , SooYoun Jun , SangHyeon Kang
IPC分类号： C12N15/00 , C12N15/64 , C12N15/70
CPC分类号： C12N15/70
摘要： The present invention relates to a PCR product cloning vector applicable directly to the cloning of a PCR product. More precisely, the present invention relates to a PCR product cloning vector designed to be effective in use for blue/white colony selection and produced based on the restriction enzyme treatment process to generate non-complementary unpaired single overhang and a method for producing the same. According to the method of the present invention, the PCR product cloning vector designed to be advantageous for blue/white cloning selection can be produced with improved efficiency compared with the convention method.
摘要翻译：本发明涉及可直接应用于PCR产物克隆的PCR产物克隆载体。更准确地说，本发明涉及设计用于蓝/白集落选择有效的PCR产物克隆载体，并且基于限制酶处理方法产生以产生非互补的未配对单突出物及其制备方法。根据本发明的方法，与常规方法相比，可以以更高的效率生产设计成有利于蓝/白克隆选择的PCR产物克隆载体。

10. 发明授权

US08951757B2 Method for selective labeling of protein produced by in vitro translation by using a marker from tRNA prepared by using in vitro transcription 有权
标题翻译：通过使用通过使用体外转录制备的来自tRNA的标记物通过体外翻译产生的蛋白质选择性标记的方法
公开(公告)号：US08951757B2
公开(公告)日：2015-02-10
申请号：US12812382
申请日：2009-01-07
申请人： Seongjun Yoon , SooYoun Jun , SangHyeon Kang
发明人： Seongjun Yoon , SooYoun Jun , SangHyeon Kang
IPC分类号： G01N33/68 , G01N33/533 , G01N33/532 , C12P19/34 , C07H21/02
CPC分类号： G01N33/533 , G01N33/532
摘要： The present invention relates to a method for the preparation of a labeling agent derived from one type of tRNA prepared by in vitro transcription, which is usable for non-radioactive selective labeling of target proteins produced by in vitro translation with fluorophore or biotin; and a method for selective labeling of target proteins produced by in vitro translation using the labeling agent without labeling pre-existing proteins in the reaction mixture.
摘要翻译：本发明涉及一种制备衍生自体外转录制备的一种类型tRNA的标记物的方法，其可用于通过用荧光团或生物素的体外翻译产生的靶蛋白的非放射性选择性标记; 以及通过使用标记试剂通过体外翻译产生的靶蛋白的选择性标记而不在反应混合物中标记预先存在的蛋白质的方法。

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