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1. 发明申请

US20070196903A1 Overcoming DAPA aminotransferase bottlenecks in biotin vitamers biosynthesis 审中-公开
标题翻译：克服生物素维生素生物合成中的DAPA氨基转移酶瓶颈
公开(公告)号：US20070196903A1
公开(公告)日：2007-08-23
申请号：US11786542
申请日：2007-04-11
申请人： Scott Van Arsdell , R. Yocum , John Perkins , Janice Pero
发明人： Scott Van Arsdell , R. Yocum , John Perkins , Janice Pero
IPC分类号： C12P17/18 , C12N9/10 , C12N1/21
CPC分类号： C07D495/04 , C12P17/186 , C12R1/125
摘要： A method is disclosed for the increased production of biotin and the biotin precursor dethiobiotin using a bacterium that produces a lysine-utilizing DAPA aminotransferase. The method involves the use of a bacterium that is either grown in the presence of lysine or deregulated for lysine biosynthesis.
摘要翻译：公开了一种使用产生赖氨酸利用DAPA氨基转移酶的细菌来增加生物素和生物素前体脱硫生物素生产的方法。该方法涉及使用在赖氨酸存在下生长或在对赖氨酸生物合成失调的细菌。

2. 发明申请

US20060099692A1 Microorganisms and processes for enhanced production of pantothenate 审中-公开
标题翻译：用于增加泛酸盐生产的微生物和方法
公开(公告)号：US20060099692A1
公开(公告)日：2006-05-11
申请号：US10520210
申请日：2002-07-03
申请人： R Yocum , Thomas Patterson , Janice Pero , Theron Hermann
发明人： R Yocum , Thomas Patterson , Janice Pero , Theron Hermann
IPC分类号： C12P19/12 , C12P13/04 , C12N1/21
CPC分类号： C07H21/04 , C12N9/0006 , C12N9/1003 , C12N9/1014 , C12N15/52 , C12P13/02
摘要： The present invention features improved methods for the enhanced production of pantoate and pantothenate utilizing microorganisms having modified pantothenate biosynthetic enzyme activities and having modified methylenetetrahydrofolate (MTF) biosynthetic enzyme activities. In particular, the invention features methods for enhancing production of desired products by increasing levels of a key intermediate, ketopantoate, by increasing enzymes or substrates that contribute directly or indirectly to its synthesis. Recombinant microorganisms and conditions for culturing same are also featured. Also featured are compositions produced by such microorganisms.
摘要翻译：本发明的特征在于利用具有改良的泛酸生物合成酶活性并具有改性的亚甲基四氢叶酸（MTF）生物合成酶活性的微生物增强生产泛酸盐和泛酸盐的改进方法。特别地，本发明的特征在于通过增加对其合成直接或间接有贡献的酶或底物来增加关键中间体酮托品酸的水平来增强所需产物的产生的方法。还具有重组微生物和培养条件。还有这些微生物产生的组合物。

3. 发明申请

US20060141585A1 Microorganisms and processes for enhanced production of pantothenate 有权
标题翻译：用于增加泛酸盐生产的微生物和方法
公开(公告)号：US20060141585A1
公开(公告)日：2006-06-29
申请号：US10520220
申请日：2003-07-03
申请人： R Yocum , Thomas Patterson , Janice Pero , Theron Hermann
发明人： R Yocum , Thomas Patterson , Janice Pero , Theron Hermann
IPC分类号： C12P13/04 , C12N1/21
CPC分类号： C12N15/52 , C12P13/02
摘要： The present invention features improved methods for the enhanced production of pantoate and pantothenate utilizing microorganisms having modified pantothenate biosynthetic enzyme activities and having modified methylenetetrahydrofolate (MTF) biosynthetic enzyme activities. In particular, the invention features methods for enhancing production of desired products by increasing levels of a key intermediate, ketopantoate, by increasing enzymes or substrates that contribute directly or indirectly to its synthesis. Recombinant microorganisms and conditions for culturing same are also are featured. Also featured are compositions produced by such microorganisms.
摘要翻译：本发明的特征在于利用具有改良的泛酸生物合成酶活性并具有改性的亚甲基四氢叶酸（MTF）生物合成酶活性的微生物增强生产泛酸盐和泛酸盐的改进方法。特别地，本发明的特征在于通过增加对其合成直接或间接有贡献的酶或底物来增加关键中间体酮托品酸的水平来增强所需产物的产生的方法。还具有重组微生物和培养条件。还有这些微生物产生的组合物。

4. 发明申请

US20050089973A1 Methods and microorganisms for production of panto-compounds 有权
标题翻译：泛化合物生产方法和微生物
公开(公告)号：US20050089973A1
公开(公告)日：2005-04-28
申请号：US10984449
申请日：2004-11-08
申请人： R. Yocum , Thomas Patterson , Theron Hermann , Janice Pero
发明人： R. Yocum , Thomas Patterson , Theron Hermann , Janice Pero
IPC分类号： C12N1/21 , C12N15/52 , C12P13/02 , C12P17/04 , C12P13/04 , C12P13/06
CPC分类号： C12P17/04 , C12N15/52 , C12P13/02
摘要： The present invention features methods of producing panto-compounds (e.g., pantothenate) using microorganisms in which the pantothenate biosynthetic pathway and/or the isoleucine-valine biosynthetic pathway and/or the coenzymeA biosynthetic pathway has been manipulated. Methods featuring ketopantoate reductase overexpressing microorganisms as well as aspartate α-decarboxylase overexpressing microorganisms are provided. Methods of producing panto-compounds in a precursor-independent manner and in high yield are described. Recombinant microorganisms, vectors, isolated nucleic acid molecules, genes and gene products useful in practicing the above methodologies are also provided. The present invention also features a previously unidentified microbial pantothenate kinase gene, coaX, as well as methods of producing panto-compounds utilizing microorganisms having modified pantothenate kinase activity. Recombinant microorganisms, vectors, isolated coaX nucleic acid molecules and purified CoaX proteins are featured. Also featured are methods for identifying pantothenate kinase modulators utilizing the recombinant microorganisms and/or purified CoaX proteins of the present invention.
摘要翻译：本发明的特征在于使用其中泛酸生物合成途径和/或异亮氨酸 - 缬氨酸生物合成途径和/或辅酶A生物合成途径已经被操纵的微生物来生产泛化合物（例如泛酸盐）的方法。本发明提供了酮过氧还原酶过表达微生物以及天冬氨酸α-脱羧酶过表达微生物的方法。描述了以前体非依赖性方式和高产率生产泛化合物的方法。还提供了用于实践上述方法的重组微生物，载体，分离的核酸分子，基因和基因产物。本发明还具有以前未鉴定的微生物泛酸激酶基因coaX，以及利用具有改良的泛酸激酶活性的微生物产生泛化合物的方法。重组微生物，载体，分离的coaX核酸分子和纯化的CoaX蛋白。还特征在于利用本发明的重组微生物和/或纯化的CoaX蛋白鉴定泛酸激酶调节剂的方法。

5. 发明申请

US20050164335A1 Methods and organisms for production of b6 vitamers 审中-公开
标题翻译：生产b6维生素的方法和生物
公开(公告)号：US20050164335A1
公开(公告)日：2005-07-28
申请号：US10508768
申请日：2003-03-21
申请人： R Yocum , Mark Williams , Janice Pero
发明人： R Yocum , Mark Williams , Janice Pero
IPC分类号： C12N1/12 , C12N1/18 , C12N1/21 , C12N9/00 , C12N9/10 , C12N15/52 , C12P17/12 , C12P25/00
CPC分类号： C12N9/00 , C12N15/52 , C12P17/12
摘要： The present invention features methods of producing B6 vitamers that involve culturing an organism overexpressing an enzyme that catalyzes a step in the biosynthesis of a B6 vitamer under conditions such that a B6 vitamer is produced. The present invention further features methods of producing B6 vitamers that involve culturing recombinant microorganisms having increased activity of at least one B6 vitamer biosynthetic enzyme, e.g., YaaD or YaaE, or a homologue thereof, or Epd, PdxA, PdxJ, PdxF, PdxB, PdxH, and/or Dxs, or a homologue thereof.
摘要翻译：本发明的特征在于生产B6维生素的方法，其涉及培养在产生B6维生素的条件下过量表达催化B6维生素生物合成步骤的酶的生物体的培养。本发明还涉及生产B6维生素的方法，其涉及培养具有增加至少一种B6维生素生物合成酶的活性的重组微生物，例如YaaD或YaaE或其同源物，或Epd，PdxA，PdxJ，PdxF，PdxB，PdxH ，和/或Dxs，或其同源物。

6. 发明授权

US5874278A Protease deletion 失效
标题翻译：蛋白酶缺失
公开(公告)号：US5874278A
公开(公告)日：1999-02-23
申请号：US842619
申请日：1997-04-15
申请人： Alan Sloma , Gerald A. Rufo, Jr. , Cathy Faye Rudolph , Barbara J. Sullivan , Janice Pero
发明人： Alan Sloma , Gerald A. Rufo, Jr. , Cathy Faye Rudolph , Barbara J. Sullivan , Janice Pero
IPC分类号： C12N9/54 , C12N1/21 , C12N9/56 , C12N15/00 , C12N15/09 , C12N15/31 , C12N15/57 , C12N15/75 , C12R1/07
CPC分类号： C12N9/54 , C12N15/00 , C12N15/75
摘要： A Bacillus cell contains a mutation in the epr gene resulting in inhibition of the production by the cell of the proteolytically active epr gene product; the cell may further contain mutations in the genes encoding proteolytically active residual protease I (RP-I) and proteolytically active residual protease II (RP-II) (mpr).
摘要翻译：芽孢杆菌细胞在epr基因中含有突变，导致细胞对蛋白水解活性的epr基因产物的产生的抑制; 细胞可能进一步含有编码蛋白水解活性残留蛋白酶I（RP-I）和蛋白水解活性残留蛋白酶II（RP-II）（mpr）的基因中的突变。

7. 发明授权

US5589383A Protease deletion 失效
标题翻译：蛋白酶缺失
公开(公告)号：US5589383A
公开(公告)日：1996-12-31
申请号：US285477
申请日：1994-08-12
申请人： Alan Sloma , Gerald A. Rufo, Jr. , Cathy F. Rudolph , Barbara J. Sullivan , Janice Pero
发明人： Alan Sloma , Gerald A. Rufo, Jr. , Cathy F. Rudolph , Barbara J. Sullivan , Janice Pero
IPC分类号： C12N9/54 , C12N1/21 , C12N9/56 , C12N15/00 , C12N15/09 , C12N15/31 , C12N15/57 , C12N15/75 , C12R1/07
CPC分类号： C12N9/54 , C12N15/00 , C12N15/75
摘要： A Bacillus cell contains a mutation in the epr gene resulting in inhibition of the production by the cell of the proteolytically active epr gene product; the cell may further contain mutations in the genes encoding proteolytically active residual protease I (bpr) and proteolytically active residual protease II (RP-II).
摘要翻译：芽孢杆菌细胞在epr基因中含有突变，导致细胞对蛋白水解活性的epr基因产物的产生的抑制; 细胞可能进一步含有编码蛋白水解活性的残留蛋白酶I（bpr）和蛋白水解活性残留蛋白酶II（RP-II）的基因中的突变。

8. 发明授权

US5017477A Enhancing DNA sequencies derived from the sacQ gene 失效
标题翻译：增强源自sacQ基因的DNA序列
公开(公告)号：US5017477A
公开(公告)日：1991-05-21
申请号：US921343
申请日：1986-10-20
申请人： Alan Sloma , Rosalind C. Lee , Janice Pero
发明人： Alan Sloma , Rosalind C. Lee , Janice Pero
IPC分类号： C07K14/58 , C12N5/00 , C12N9/26 , C12N9/28 , C12N9/50 , C12N9/54 , C12N15/00 , C12N15/67 , C12N15/75 , C12P19/34
CPC分类号： C12N9/2417 , C07K14/58 , C12N15/67 , C12N15/75 , C12N9/54 , C07K2319/00 , C07K2319/02
摘要： A bacterial cell transformed with a gene encoding a desired product and with an enhancing DNA sequence capable of enhancing the production of the desired product in the bacterial cell, the enhancing DNA sequence being further characterized in that it is capable of enhancing the production of an endogenous protease in a Gram-positive bacterial cell.
摘要翻译：用编码所需产物的基因和能够增强细菌细胞中所需产物产生的增强DNA序列转化的细菌细胞，进一步的特征在于其能够增强内源性的产生蛋白酶在革兰氏阳性细菌细胞中。

9. 发明授权

US5188961A DNA encoding a Streptomyces endochitinase 56 signal peptide 失效
标题翻译：编码链霉菌内切素酶56信号肽的DNA
公开(公告)号：US5188961A
公开(公告)日：1993-02-23
申请号：US473309
申请日：1990-02-01
申请人： Karen M. Overbye , Janice Pero , Phillips W. Robbins
发明人： Karen M. Overbye , Janice Pero , Phillips W. Robbins
IPC分类号： A01N63/00 , C12N9/24 , C12N15/62 , C12N15/76
CPC分类号： C12N15/76 , A01N63/00 , C12N15/625 , C12N9/2442 , C12Y302/01014 , C07K2319/02 , C07K2319/036 , C07K2319/61
摘要： Engineered DNA encoding a pre-protein which comprises a Streptomyces chitinase signal sequence and a desired mature protein. Preferably, the signal sequence is from the S. plicatus endochitinase gene. The engineered DNA is included in a host organism causing the host to produce and secrete the mature protein. Where the mature protein is a chitinase, the invention is used in methods of controlling chitinase-sensitive pests.
摘要翻译：编码包含链霉菌几丁质酶信号序列和期望成熟蛋白质的前蛋白质的工程DNA。优选地，信号序列来自S.plicatus内切蛋白酶基因。工程DNA包含在宿主生物体中，导致宿主产生和分泌成熟蛋白质。当成熟蛋白质是几丁质酶时，本发明用于控制几丁质酶敏感性害虫的方法。

10. 发明授权

US4745056A Streptomyces secretion vector 失效
标题翻译：链霉菌分泌载体
公开(公告)号：US4745056A
公开(公告)日：1988-05-17
申请号：US663842
申请日：1984-10-23
申请人： Sonia Guterman , Janice Pero , Phillips Robbins
发明人： Sonia Guterman , Janice Pero , Phillips Robbins
IPC分类号： C12N15/09 , C12N1/20 , C12N9/16 , C12N9/24 , C12N15/76 , C12P21/02 , C12R1/465 , C12P21/00 , C12N7/00 , C12N15/00
CPC分类号： C12Y302/01096 , C12N15/76 , C12N9/16 , C12Y301/03001 , C07K2319/02 , Y10S435/886 , Y10S435/896 , Y10S930/30
摘要： Cloning vectors are disclosed to obtain secretion of a desired protein from a host Streptomyces when a structural gene coding for the protein is inserted into the vector. The vector has: (1) regulatory DNA that includes a promoter sequence effective to start transcription in the host Streptomyces and DNA that encodes a ribosome-binding site; (2) a DNA sequence that codes for a signal sequence that occurs naturally in a Streptomyces strain or that derives from such a DNA sequence; and (3) at least one engineered restriction endonuclease recognition site positioned for the insertion of a structural gene, the DNA that encodes a signal sequence and attached structural gene being transcribed and translated together under the control of the regulatory DNA. Expression vectors are disclosed which include a structural gene coding for a desired protein so positioned. Streptomyces cells containing the vector and methods of using them to produce the desired protein are disclosed. Finally, an alkaline phosphatase gene is inserted in secretion vectors for use in a method of evaluating the ability of those vectors to cause secretion in host gram positive bacteria; the method may be used to evaluate either a potential host or a potential secretion vector.
摘要翻译：当编码蛋白质的结构基因插入载体时，公开了克隆载体以从宿主链霉菌中分泌所需的蛋白质。该载体具有：（1）包含启动子序列的调控DNA，该启动子序列有效启动宿主链霉菌和编码核糖体结合位点的DNA; （2）编码在链霉菌属菌株中天然存在或源自这种DNA序列的信号序列的DNA序列; 和（3）至少一个设计用于插入结构基因的工程化的限制性内切核酸酶识别位点，编码信号序列的DNA和附着的结构基因，在调节DNA的控制下被转录和翻译。公开了包括编码所定位的所需蛋白质的结构基因的表达载体。公开了含有载体的链霉菌细胞和使用它们产生所需蛋白质的方法。最后，将碱性磷酸酶基因插入分泌载体中，用于评估这些载体在宿主革兰氏阳性细菌中分泌能力的方法; 该方法可以用于评估潜在的宿主或潜在的分泌载体。

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