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1. 发明申请

US20120213905A1 AQUACULTURE FEED COMPOSITIONS 审中-公开
标题翻译：水产养殖组合物
公开(公告)号：US20120213905A1
公开(公告)日：2012-08-23
申请号：US13208070
申请日：2011-08-11
申请人： Scott E. Nichols , J. Martin Odom , Pamela L. Sharpe
发明人： Scott E. Nichols , J. Martin Odom , Pamela L. Sharpe
IPC分类号： A23K1/18 , A23K1/16 , A23K1/10
CPC分类号： A23K50/80 , A23K10/22 , A23K20/158 , A23K20/179 , A23K40/20 , A23K40/25 , Y02A40/818
摘要： Aquaculture feed compositions having a ratio of concentration of omega-3 polyunsaturated fatty acids of eicosapentaenoic acid [“EPA”; cis-5,8,11,14,17-eicosapentaenoic acid; 20:5] to concentration of docosahexaenoic acid [“DHA”; cis-4,7,10,13,16,19-docosahexaenoic acid; 22:6] greater than 2:1 based on the individual concentrations of EPA and DHA in the aquaculture feed composition, and having a biomass source of carotenoid(s), are disclosed. Furthermore, such aquaculture feed compositions may have a concentration of the sum of EPA and DHA that is at least about 0.8%, measured as a weight percent of the aquaculture feed composition.
摘要翻译：具有二十碳五烯酸的ω-3多不饱和脂肪酸的浓度比的水产养殖饲料组合物[“EPA”; 顺-5,8,11,14,17-二十碳五烯酸; 20：5]浓缩二十二碳六烯酸[“DHA”; 顺-4,7,10,13,16,19-二十二碳六烯酸; 22：6]大于2：1，基于水产养殖饲料组合物中EPA和DHA的个体浓度，并具有类胡萝卜素的生物质来源。此外，这样的水产养殖饲料组合物可以具有至少约0.8％的EPA和DHA之和的浓度，以水产养殖饲料组合物的重量百分比测量。

2. 发明授权

US08658413B2 Mutant HPGG motif and HDASH motif delta-5 desaturases and their use in making polyunsaturated fatty acids 有权
标题翻译：突变型HPGG基序和HDASH基序delta-5去饱和酶及其在制备多不饱和脂肪酸中的应用
公开(公告)号：US08658413B2
公开(公告)日：2014-02-25
申请号：US13218673
申请日：2011-08-26
申请人： Melissa D. Bosak , Michael W. Bostick , Hongxian He , Seung-Pyo Hong , Dana M. Walters Pollak , Pamela L. Sharpe , Yehong Jamie Wang , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
发明人： Melissa D. Bosak , Michael W. Bostick , Hongxian He , Seung-Pyo Hong , Dana M. Walters Pollak , Pamela L. Sharpe , Yehong Jamie Wang , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
IPC分类号： C12N1/20 , C12N9/00 , C07H21/04
CPC分类号： C12N9/001 , C12P7/6472 , C12Y103/01035
摘要： Mutant delta-5 desaturases, having the ability to convert dihomo-gamma-linolenic acid [DGLA; 20:3 omega-6] to arachidonic acid [ARA; 20:4 omega-6] and/or eicosatetraenoic acid [ETA; 20:4 omega-3] to eicosapentaenoic acid [EPA; 20:5 omega-3] and possessing at least one mutation within the HPGG (SEQ ID NO:7) motif of the cytochome b5-like domain and at least one mutation within the HDASH (SEQ ID NO:8) motif are disclosed. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-5 desaturases, along with a method of making long chain polyunsaturated fatty acids [“PUFAs”], are also disclosed.
摘要翻译：具有将dihomo-γ-亚麻酸转化的能力的变体delta-5去饱和酶[DGLA; 20：3ω-6]花生四烯酸[ARA; 20：4ω-6]和/或二十碳四烯酸[ETA; 20：4ω-3]至二十碳五烯酸[EPA; 20：5ω-3]，并且在细胞色素b5样结构域的HPGG（SEQ ID NO：7）基序和HDASH（SEQ ID NO：8）基序内的至少一个突变中具有至少一个突变。还公开了分离的核酸片段和包含编码Δ-5去饱和酶的片段的重组构建物，以及制备长链多不饱和脂肪酸[“PUFA”]的方法。

3. 发明授权

US07217537B2 Method to increase carotenoid production in a microbial host cell by down-regulating glycogen synthase 失效
公开(公告)号：US07217537B2
公开(公告)日：2007-05-15
申请号：US11227613
申请日：2005-09-15
申请人： Edward S. Miller, Jr. , James M. Odom , Pamela L. Sharpe
发明人： Edward S. Miller, Jr. , James M. Odom , Pamela L. Sharpe
IPC分类号： C12P1/00 , C12P23/00 , C12P31/00 , C12N15/70
CPC分类号： C12N9/1051 , C12P23/00
摘要： A method to increase carotenoid production in carotenogenic microbial host cells is provided by down-regulating or disrupting glycogen synthesis. Disruption of glycogen synthase activity in a carotenogenic microbial host cell significantly increased carotenoid production. Carotenogenic microorganisms are also provided that have been optimized for the production of carotenoid compounds through the down-regulation and/or disruption of glycogen synthase activity.

4. 发明授权

US06673567B2 Method of determination of gene function 失效
标题翻译：测定基因功能的方法
公开(公告)号：US06673567B2
公开(公告)日：2004-01-06
申请号：US09808658
申请日：2001-03-15
申请人： Pamela L. Sharpe , Vasantha Nagarajan
发明人： Pamela L. Sharpe , Vasantha Nagarajan
IPC分类号： C12Q100
CPC分类号： C12N15/1082 , C12N15/1079 , C12Q1/6897
摘要： The present method is useful for the identification of genes, ORF's and other nucleic acid molecules which are essential for the expression of a specific phenotype in microorganisms. The method employs In vitro transposition in conjunction with an chromosomal integration vector containing a specific gene or genetic element whose function is unknown. Subsequent transformation of a recombination proficient host with the vector and growth first under non-integrating conditions and then under integrating conditions, followed by a selection screen for either single or double crossover events results in transformants that may be subjected to phenotypic screens to determine gene function.
摘要翻译：本发明方法可用于鉴定在微生物中表达特定表型所必需的基因ORF和其它核酸分子。该方法使用体外转座与包含功能未知的特定基因或遗传元件的染色体整合载体。随后，在非整合条件下，然后在整合条件下，首先用载体和生长进行重组熟练宿主的转化，然后在单一或双交换事件的选择筛选中导致可以进行表型筛选以确定基因功能的转化体。

5. 发明授权

US08637298B2 Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production 有权
标题翻译：优化的解脂耶氏酵母菌株用于高二十碳五烯酸生产
公开(公告)号：US08637298B2
公开(公告)日：2014-01-28
申请号：US12814815
申请日：2010-06-14
申请人： Seung-Pyo Hong , Pamela L. Sharpe , Zhixiong Xue , Narendra S. Yadav , Quinn Qun Zhu
发明人： Seung-Pyo Hong , Pamela L. Sharpe , Zhixiong Xue , Narendra S. Yadav , Quinn Qun Zhu
IPC分类号： C12N1/00
CPC分类号： C12N9/1029 , A21D2/165 , A23K20/158 , C12N9/00 , C12N9/13 , C12N9/93 , C12P7/6427 , C12P7/6472 , C12P7/6481
摘要： Described are engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing an oil comprising greater than 50 weight percent of eicosapentaenoic acid [“EPA”], an ω-3 polyunsaturated fatty acid, measured as a weight percent of total fatty acids [“% TFAs”] and having a ratio of at least 3.1 of EPA % TFAs, to linoleic acid, measured as % TFAs. These strains over-express at least one Δ9 elongase/Δ8 desaturase multizyme, in addition to other heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases, C16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases, malonyl CoA synthetases and/or acyl-CoA lysophospholipid acyltransferases. The expression of at least one peroxisome biogenesis factor protein is down-regulated. Methods for producing EPA within said host cells, oils obtained from the cells, and products therefrom are claimed.
摘要翻译：描述了能够产生包含大于50重量％的二十碳五烯酸[“EPA”]的油的油质酵母解脂耶氏酵母解脂耶氏酵母的工程菌株，其是以总脂肪酸的重量百分比测量的ω-3多不饱和脂肪酸[“％ TFAs“]，并且具有至少3.1％的EPA％TFA与比率为TFA的亚油酸的比率。除了其他异源Delta9延伸酶，Delta8去饱和酶，Δ5去饱和酶，Δ17去饱和酶，Δ12去饱和酶，C16 / 18延长酶和任选的过表达二酰基甘油胆碱磷酸转移酶，丙二酰辅酶A，这些菌株过表达至少一种Δ9延伸酶/Δδ去饱和酶多酶合成酶和/或酰基辅酶A溶血磷脂酰基转移酶。至少一种过氧化物酶体生物因子蛋白的表达下调。要求在所述宿主细胞内生产EPA的方法，从细胞获得的油及其产物。

6. 发明授权

US07232666B2 Optimized bacterial host strains of Methylomonas sp. 16a 失效
标题翻译：优化的细菌宿主菌株Methylomonas sp。 16a
公开(公告)号：US07232666B2
公开(公告)日：2007-06-19
申请号：US10997844
申请日：2004-11-24
申请人： Pamela L. Sharpe , Qiong Cheng , Melissa D. Bosak , Luan Tao , Natalia Sedkova
发明人： Pamela L. Sharpe , Qiong Cheng , Melissa D. Bosak , Luan Tao , Natalia Sedkova
IPC分类号： C12P23/00 , C07H21/04 , C12N9/10 , C12N15/74 , C12N1/21
CPC分类号： C12N9/001 , C12N9/00 , C12N9/0004 , C12N9/0071 , C12N9/0083 , C12N9/1051 , C12N9/1085 , C12N9/90 , C12N15/52 , C12N15/74 , C12P23/00 , C12R1/26 , C12Y205/01029
摘要： Methanotrophic bacterial strains are provided that have been optimized for the production of carotenoid compounds through the down-regulation of one or more of the crtN1, ald, crtN2 and crtN3 genes of the carotenoid biosynthetic pathway. The resulting strains lack pigmented C30 carotenoid compounds, and show an increase in the production of C40 carotenoids. The use of the optimized host strains for the production of the C40 carotenoids canthaxanthin and astaxanthin is also described.
摘要翻译：提供了通过下调类胡萝卜素生物合成途径的一个或多个crtN1，ald，crtN2和crtN3基因来优化用于生产类胡萝卜素化合物的甲烷营养型细菌菌株。所得的菌株缺乏C 30 N类胡萝卜素化合物的色素沉着，并显示出C 40 类胡萝卜素的产生增加。还描述了优化的宿主菌株用于生产C 40 H类胡萝卜素角黄素和虾青素的用途。

7. 发明授权

US08703473B2 Recombinant microbial host cells for high eicosapentaenoic acid production 有权
标题翻译：用于高二十碳五烯酸生产的重组微生物宿主细胞
公开(公告)号：US08703473B2
公开(公告)日：2014-04-22
申请号：US13218708
申请日：2011-08-26
申请人： Seung-Pyo Hong , Pamela L. Sharpe , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
发明人： Seung-Pyo Hong , Pamela L. Sharpe , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
IPC分类号： C12N1/00 , C12N1/20 , C12P7/64
CPC分类号： C12N9/1029 , C12N9/0071 , C12N15/815 , C12P7/6472
摘要： Engineered strains of the oleaginous yeast Yarrowia lipolytica are disclosed herein that are capable of producing microbial oil comprising greater than 25 weight percent of eicosapentaenoic acid [“EPA”], an omega-3 polyunsaturated fatty acid, measured as a weight percent of dry cell weight.
摘要翻译：本文公开了含油酵母解脂耶氏酵母解脂耶氏酵母的工程菌株，其能够产生包含大于25重量％的二十碳五烯酸[“EPA”]的微生物油，其为以干细胞重量的重量百分比测量的ω-3多不饱和脂肪酸。

8. 发明授权

US08399226B2 High eicosapentaenoic acid oils from improved optimized strains of Yarrowia lipolytica 有权
标题翻译：来自改良的解脂耶氏酵母菌株的高二十碳五烯酸油
公开(公告)号：US08399226B2
公开(公告)日：2013-03-19
申请号：US12814880
申请日：2010-06-14
申请人： Seung-Pyo Hong , Pamela L. Sharpe , Zhixiong Xue , Narendra S. Yadav , Quinn Qun Zhu
发明人： Seung-Pyo Hong , Pamela L. Sharpe , Zhixiong Xue , Narendra S. Yadav , Quinn Qun Zhu
IPC分类号： C12P7/64 , A23D9/00
CPC分类号： C12N9/1029 , A21D2/165 , A23K20/158 , C12N9/00 , C12N9/13 , C12N9/93 , C12P7/6427 , C12P7/6472 , C12P7/6481
摘要： Described are engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing an oil comprising greater than 50 weight percent of eicosapentaenoic acid [“EPA”], an ω-3 polyunsaturated fatty acid, measured as a weight percent of total fatty acids [“% TFAs”] and having a ratio of at least 3.1 of EPA % TFAs, to linoleic acid, measured as % TFAs. These strains over-express at least one Δ9 elongase/Δ8 desaturase multizyme, in addition to other heterologous Δ9 elongases, Δ8 desaturases, Δ5 desaturases, Δ17 desaturases, Δ12 desaturases, C16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases, malonyl CoA synthetases and/or acyl-CoA lysophospholipid acyltransferases. The strains possess at least one peroxisome biogenesis factor protein knockout. Methods for producing EPA within said host cells, oils obtained from the cells, and products therefrom are claimed.
摘要翻译：描述了能够产生包含大于50重量％的二十碳五烯酸[EPA]，ω-3多不饱和脂肪酸的脂肪酵母解脂耶氏酵母的工程菌株，以总脂肪酸的重量％[％TFA]和具有至少3.1％的EPA％TFA与亚油酸的比例，以％TFA测定。除了其他异源＆Dgr .9延伸酶，Dgr。8去饱和酶，Dgr。5去饱和酶，Dgr 17脱饱和酶，Dgr。12去饱和酶，C16外，这些菌株过度表达至少一种Dgr 9延伸酶/ / 18延伸酶和任选的过表达二酰基甘油胆碱磷酸转移酶，丙二酰辅酶A合成酶和/或酰基辅酶A溶血磷脂酰基转移酶。该菌株具有至少一种过氧化物酶体生物发生因子蛋白质敲除。要求在所述宿主细胞内生产EPA的方法，从细胞获得的油及其产物。

9. 发明授权

US07504236B2 Method to increase carotenoid production in a microbial host cell by down-regulating glycogen synthase 失效
标题翻译：通过下调糖原合成酶来增加微生物宿主细胞中类胡萝卜素产生的方法
公开(公告)号：US07504236B2
公开(公告)日：2009-03-17
申请号：US11732165
申请日：2007-04-02
申请人： Edward S. Miller, Jr. , James M. Odom , Pamela L. Sharpe
发明人： Edward S. Miller, Jr. , James M. Odom , Pamela L. Sharpe
IPC分类号： C12P23/00 , C12N1/13
CPC分类号： C12N9/1051 , C12P23/00
摘要： A method to increase carotenoid production in carotenogenic microbial host cells is provided by down-regulating or disrupting glycogen synthesis. Disruption of glycogen synthase activity in a carotenogenic microbial host cell significantly increased carotenoid production. Carotenogenic microorganisms are also provided that have been optimized for the production of carotenoid compounds through the down-regulation and/or disruption of glycogen synthase activity.
摘要翻译：通过下调或破坏糖原合成来提供在胡萝卜素微生物宿主细胞中增加类胡萝卜素产生的方法。致癌微生物宿主细胞中糖原合成酶活性的破坏显着增加类胡萝卜素的产生。还提供了通过下调和/或破坏糖原合成酶活性而优化用于生产类胡萝卜素化合物的致细性微生物。

10. 发明申请

US20120142082A1 CAROTENOID PRODUCTION IN A RECOMBINANT OLEAGINOUS YEAST 有权
标题翻译：在重组油脂中生产碳酸钙
公开(公告)号：US20120142082A1
公开(公告)日：2012-06-07
申请号：US11952243
申请日：2007-12-07
申请人： Pamela L. Sharpe , Rick W. Ye , Quinn Qun Zhu
发明人： Pamela L. Sharpe , Rick W. Ye , Quinn Qun Zhu
IPC分类号： C12N1/19 , A23K1/18 , A23L1/27 , C12P23/00 , A23K1/00
CPC分类号： C12P23/00 , C07C403/24 , C09B61/00 , C12P7/6427 , C12P7/6472
摘要： Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing carotenoids (e.g., β-carotene, lycopene, lutein, zeaxanthin, canthaxanthin, astaxanthin) are provided. The strains may also be engineered to co-produce at least one ω-3/ω-6 polyunsaturated fatty acid and/or at least one additional antioxidant. Methods of using the carotenoid products obtained (e.g., biomass and/or pigmented oils) in food and feed applications are also provided.
摘要翻译：提供能够产生类胡萝卜素（例如，胡萝卜素，番茄红素，叶黄素，玉米黄质，角黄素，虾青素）的含油酵母解脂耶氏酵母解脂耶氏酵母菌的工程菌株。菌株也可以被工程化以共同产生至少一种ω-3 /ω-6多不饱和脂肪酸和/或至少一种另外的抗氧化剂。还提供了在食品和饲料应用中使用获得的类胡萝卜素产物（例如生物质和/或着色油）的方法。

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