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    • 1. 发明申请
      US20090221501A1 MIXING AND MATCHING TC PROTEINS FOR PEST CONTROL 有权
    • MIXING AND MATCHING TC PROTEINS FOR PEST CONTROL
    • 混合和匹配TC蛋白进行病虫害防治
    • US20090221501A1
    • 2009-09-03
    • US12371825
    • 2009-02-16
    • Timothy D. Hey , Amanda D. Schleper , Scott A. Bevan , Scott B. Bintrim , Jon C. Mitchell , Ze Sheng Li , Weiting Ni , Baolong Zhu , Donald J. Merlo , Patricia C. Apel-Birkhold , Thomas Meade
    • Timothy D. Hey , Amanda D. Schleper , Scott A. Bevan , Scott B. Bintrim , Jon C. Mitchell , Ze Sheng Li , Weiting Ni , Baolong Zhu , Donald J. Merlo , Patricia C. Apel-Birkhold , Thomas Meade
    • A61K38/16 , A01P7/04
    • C07K14/195 , A01N37/46 , A01N63/02 , C07K14/24 , C12N15/8286 , Y02A40/162
    • The subject invention relates to the surprising discovery that toxin complex (TC) proteins, obtainable from Xenorhabdus, Photorhabdus, and Paenibacillus, can be used interchangeably with each other. In particularly preferred embodiments of the subject invention, the toxicity of a “stand-alone” TC protein (from Photorhabdus, Xenorhabdus, or Paenibacillus, for example) is enhanced by one or more TC protein “potentiators” derived from a source organism of a different genus from which the toxin was derived. As one skilled in the art will recognize with the benefit of this disclosure, this has broad implications and expands the range of utility that individual types of TC proteins will now be recognized to have. Among the most important advantages is that one skilled in the art will now be able to use a single set of potentiators to enhance the activity of a stand-alone Xenorhabdus protein toxin as well as a stand-alone Photorhabdus protein toxin. (As one skilled in the art knows, Xenorhabdus toxin proteins tend to be more desirable for controlling lepidopterans while Photorhabdus toxin proteins tend to be more desirable for controlling coleopterans.) This reduces the number of genes, and transformation events, needed to be expressed by a transgenic plant to achieve effective control of a wider spectrum of target pests. Certain preferred combinations of heterologous TC proteins are also disclosed herein. Other objects, advantages, and features of the subject invention will be apparent to one skilled in the art having the benefit of the subject disclosure.
    • 本发明涉及令​​人惊奇的发现，可以从Xenorhabdus，Photorhabdus和Paenibacillus获得的毒素复合物（TC）蛋白质彼此互换使用。 在本发明的特别优选的实施方案中，“独立”TC蛋白（例如，来自Photorhabdus，Xenorhabdus或Paenibacillus的例如）的毒性由一种或多种衍生自以下的源生物体的TC蛋白“增强剂”增强 来源于毒素的不同属。 本领域技术人员将会认识到本公开的益处，这具有广泛的含义并扩大了现在将认识到各种类型的TC蛋白质具有的效用范围。 最重要的优点之一是，本领域技术人员现在将能够使用单一增强剂来增强独立的Xenorhabdus蛋白毒素的活性以及独立的Photorhabdus蛋白毒素。 （如本领域技术人员所知，Xenorhabdus毒素蛋白质倾向于更好地控制鳞翅目，而Photorhabdus毒素蛋白质倾向于更有利于控制鞘翅目）。这减少了需要表达的基因数量和转化事件 一个转基因植物，以实现更广泛的目标害虫的有效控制。 异源TC蛋白的某些优选组合也在本文中公开。 受益于本发明的本发明的其它目的，优点和特征对本领域技术人员而言是显而易见的。
    • 基本信息 添加关注 加入工作空间 PDF全文 PDF下载 全文下载 相似专利 双屏查看 权利要求书 说明书全文 Global Dossier Espacenet 法律信息 同族专利 专利引用
    • 2. 发明授权
      US07491698B2 Mixing and matching TC proteins for pest control 有权
    • Mixing and matching TC proteins for pest control
    • 混合和匹配TC蛋白用于病虫害防治
    • US07491698B2
    • 2009-02-17
    • US10754115
    • 2004-01-07
    • Timothy D. Hey , Amanda D. Schleper , Scott A. Bevan , Scott B. Bintrim , Jon C. Mitchell , Ze Sheng Li , Weiting Ni , Baolong Zhu , Donald J. Merlo , Patricia C. Apel-Birkhold , Thomas Meade
    • Timothy D. Hey , Amanda D. Schleper , Scott A. Bevan , Scott B. Bintrim , Jon C. Mitchell , Ze Sheng Li , Weiting Ni , Baolong Zhu , Donald J. Merlo , Patricia C. Apel-Birkhold , Thomas Meade
    • A61K38/16 , A01N33/04 , C07K14/195
    • C07K14/195 , A01N37/46 , A01N63/02 , C07K14/24 , C12N15/8286 , Y02A40/162
    • The subject invention relates to the surprising discovery that toxin complex (TC) proteins, obtainable from Xenorhabdus, Photorhabdus, and Paenibacillus, can be used interchangeably with each other. In particularly preferred embodiments of the subject invention, the toxicity of a “stand-alone” TC protein (from Photorhabdus, Xenorhabdus, or Paenibacillus, for example) is enhanced by one or more TC protein “potentiators” derived from a source organism of a different genus from which the toxin was derived. As one skilled in the art will recognize with the benefit of this disclosure, this has broad implications and expands the range of utility that individual types of TC proteins will now be recognized to have. Among the most important advantages is that one skilled in the art will now be able to use a single set of potentiators to enhance the activity of a stand-alone Xenorhabdus protein toxin as well as a stand-alone Photorhabdus protein toxin. (As one skilled in the art knows, Xenorhabdus toxin proteins tend to be more desirable for controlling lepidopterans while Photorhabdus toxin proteins tend to be more desirable for controlling coleopterans.) This reduces the number of genes, and transformation events, needed to be expressed by a transgenic plant to achieve effective control of a wider spectrum of target pests. Certain preferred combinations of heterologous TC proteins are also disclosed herein. Other objects, advantages, and features of the subject invention will be apparent to one skilled in the art having the benefit of the subject disclosure.
    • 本发明涉及令​​人惊奇的发现，可以从Xenorhabdus，Photorhabdus和Paenibacillus获得的毒素复合物（TC）蛋白质彼此互换使用。 在本发明的特别优选的实施方案中，“独立”TC蛋白（例如，来自Photorhabdus，Xenorhabdus或Paenibacillus的例如）的毒性由一种或多种衍生自以下的源生物体的TC蛋白“增强剂”增强 来源于毒素的不同属。 本领域技术人员将会认识到本公开的益处，这具有广泛的含义并扩大了现在将认识到各种类型的TC蛋白质具有的效用范围。 最重要的优点之一是，本领域技术人员现在将能够使用单一增强剂来增强独立的Xenorhabdus蛋白毒素的活性以及独立的Photorhabdus蛋白毒素。 （如本领域技术人员所知，Xenorhabdus毒素蛋白质倾向于更好地控制鳞翅目，而Photorhabdus毒素蛋白质倾向于更有利于控制鞘翅目）。这减少了需要表达的基因数量和转化事件 一个转基因植物，以实现更广泛的目标害虫的有效控制。 异源TC蛋白的某些优选组合也在本文中公开。 受益于本发明的本发明的其它目的，优点和特征对本领域技术人员而言是显而易见的。
    • 基本信息 添加关注 加入工作空间 PDF全文 PDF下载 全文下载 相似专利 双屏查看 权利要求书 说明书全文 Espacenet 法律信息 同族专利 专利引用
    • 3. 发明授权
      US08084418B2 Methods of inhibiting insects by treatment with a complex comprising a Photorhabdus insecticidal protein and one or two Xenorhabdus enhancer proteins 有权
    • Methods of inhibiting insects by treatment with a complex comprising a Photorhabdus insecticidal protein and one or two Xenorhabdus enhancer proteins
    • 通过用包含Photorhabdus杀虫蛋白和一种或两种Xenorhabdus增强子蛋白的复合物处理来抑制昆虫的方法
    • US08084418B2
    • 2011-12-27
    • US12371825
    • 2009-02-16
    • Timothy D. Hey , Amanda D. Schleper , Scott A. Bevan , Scott B. Bintrim , Jon C. Michell , Ze Sheng Li , Weiting Ni , Baolong Zhu , Donald J. Merlo , Patricia C. Apel-Birkhold , Thomas Meade
    • Timothy D. Hey , Amanda D. Schleper , Scott A. Bevan , Scott B. Bintrim , Jon C. Michell , Ze Sheng Li , Weiting Ni , Baolong Zhu , Donald J. Merlo , Patricia C. Apel-Birkhold , Thomas Meade
    • A01N33/04 , A01N25/00 , A61K38/16 , C07K14/195
    • C07K14/195 , A01N37/46 , A01N63/02 , C07K14/24 , C12N15/8286 , Y02A40/162
    • The subject invention relates to the surprising discovery that toxin complex (TC) proteins, obtainable from Xenorhabdus, Photorhabdus, and Paenibacillus, can be used interchangeably with each other. In particularly preferred embodiments of the subject invention, the toxicity of a “stand-alone” TC protein (from Photorhabdus, Xenorhabdus, or Paenibacillus, for example) is enhanced by one or more TC protein “potentiators” derived from a source organism of a different genus from which the toxin was derived. As one skilled in the art will recognize with the benefit of this disclosure, this has broad implications and expands the range of utility that individual types of TC proteins will now be recognized to have. Among the most important advantages is that one skilled in the art will now be able to use a single set of potentiators to enhance the activity of a stand-alone Xenorhabdus protein toxin as well as a stand-alone Photorhabdus protein toxin. (As one skilled in the art knows, Xenorhabdus toxin proteins tend to be more desirable for controlling lepidopterans while Photorhabdus toxin proteins tend to be more desirable for controlling coleopterans.) This reduces the number of genes, and transformation events, needed to be expressed by a transgenic plant to achieve effective control of a wider spectrum of target pests. Certain preferred combinations of heterologous TC proteins are also disclosed herein. Other objects, advantages, and features of the subject invention will be apparent to one skilled in the art having the benefit of the subject disclosure.
    • 本发明涉及令​​人惊奇的发现，可以从Xenorhabdus，Photorhabdus和Paenibacillus获得的毒素复合物（TC）蛋白质彼此互换使用。 在本发明的特别优选的实施方案中，“独立”TC蛋白（例如，来自Photorhabdus，Xenorhabdus或Paenibacillus的例如）的毒性由一种或多种衍生自以下物质的来源生物体的TC蛋白“增强剂”增强 来源于毒素的不同属。 本领域技术人员将会认识到本公开的益处，这具有广泛的含义并扩大了现在将认识到各种类型的TC蛋白质具有的效用范围。 最重要的优点之一是，本领域技术人员现在将能够使用单一增强剂来增强独立的Xenorhabdus蛋白毒素的活性以及独立的Photorhabdus蛋白毒素。 （如本领域技术人员所知，Xenorhabdus毒素蛋白质倾向于更好地控制鳞翅目，而Photorhabdus毒素蛋白质倾向于更有利于控制鞘翅目）。这减少了需要表达的基因数量和转化事件 一个转基因植物，以实现更广泛的目标害虫的有效控制。 异源TC蛋白的某些优选组合也在本文中公开。 受益于本发明的本发明的其它目的，优点和特征对本领域技术人员而言是显而易见的。
    • 基本信息 添加关注 加入工作空间 PDF全文 PDF下载 全文下载 相似专利 双屏查看 权利要求书 说明书全文 Espacenet 法律信息 同族专利 专利引用
    • 4. 发明授权
      US07902334B2 Pesticidally active proteins and polynucleotides obtainable from Paenibacillus species 有权
    • Pesticidally active proteins and polynucleotides obtainable from Paenibacillus species
    • 杀虫活性蛋白质和可从Paenibacillus物种获得的多核苷酸
    • US07902334B2
    • 2011-03-08
    • US11775663
    • 2007-07-10
    • Scott B. Bintrim , Scott A. Bevan , Baolong Zhu , Donald J. Merlo
    • Scott B. Bintrim , Scott A. Bevan , Baolong Zhu , Donald J. Merlo
    • C07K14/00
    • C12P19/34 , C07K14/195 , C12P21/02 , C12Q1/689 , C12Q2600/142
    • The subject invention provides unique biological alternatives for pest control. More specifically, the present invention relates to novel pesticidal proteins, novel sources of pesticidal proteins, polynucleotides that encode such toxins, and to methods of using these toxins to control insects and other plant pests. The subject invention relates to the surprising discovery that Paenibacillus species, and proteins therefrom, have toxicity to lepidopterans. There have been no known reports of a Paenibacillus species, strain, or protein having toxicity to lepidopterans. This is also the first known example of a Paenibacillus Cry protein that is toxic to lepidopterans. Furthermore, this is the first known report of a Paenibacillus having toxin complex (TC)-like proteins. The DAS1529 isolate disclosed here is also the first known example of a natural bacterium that produces both a Cry toxin and TC proteins. The subject invention also relates to new classes of Cry and TC proteins that are pesticidally active.
    • 本发明提供了用于病虫害防治的独特的生物学替代物。 更具体地，本发明涉及新型杀虫蛋白，杀虫蛋白的新来源，编码这种毒素的多核苷酸，以及使用这些毒素来控制昆虫和其它植物害虫的方法。 本发明涉及令​​人惊奇的发现：Paenibacillus物种及其蛋白质对鳞翅目病毒具有毒性。 没有已知的具有对鳞翅目昆虫具有毒性的Paenibacillus物种，菌株或蛋白质的报道。 这也是对鳞翅目昆虫具有毒性的第一个已知的Paenibacillus Cry蛋白的例子。 此外，这是具有毒素复合物（TC）样蛋白质的Paenibacillus的第一个已知报告。 此处公开的DAS1529分离物也是产生Cry毒素和TC蛋白质的天然细菌的第一已知实例。 本发明还涉及具有杀虫活性的新类型的Cry和TC蛋白。
    • 基本信息 添加关注 加入工作空间 PDF全文 PDF下载 全文下载 相似专利 双屏查看 权利要求书 说明书全文 Espacenet 法律信息 同族专利 专利引用
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