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1. 发明申请

US20130090470A1 OPTICALLY-DETECTABLE ENZYME SUBSTRATES AND THEIR METHOD OF USE 有权
标题翻译：光学检测酶基质及其使用方法
公开(公告)号：US20130090470A1
公开(公告)日：2013-04-11
申请号：US13614948
申请日：2012-09-13
申请人： Schuyler CORRY , William Downey , Brian Filanoski , Kyle Gee , Lawrence Greenfield , James Hirsch , Iain Johnson , Aleksey Rukavishnikov
发明人： Schuyler CORRY , William Downey , Brian Filanoski , Kyle Gee , Lawrence Greenfield , James Hirsch , Iain Johnson , Aleksey Rukavishnikov
IPC分类号： C07D503/00 , C07F5/02 , C07D501/58
CPC分类号： C07F5/022 , C07D499/00 , C07D501/00 , C07D501/58 , C07D501/60 , C07D503/00 , C07D519/00 , C12Q1/34 , C12Q2334/00 , G01N33/581 , G01N2333/986
摘要： The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a β-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a β-lactmase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a β-lactmase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a β-lactmase or instructions for using the components in an assay.
摘要翻译：本发明涉及作为酶的底物的化合物，并且在与酶反应时提供可检测的反应，例如可光学检测的反应。特别地，这些化合物可用于检测样品中β-内酰胺酶的存在。除了化合物之外，还公开了分析样品中存在β-乳糖酶的方法，例如，作为包括编码β-乳糖酶的序列的核酸序列的表达的指示剂。公开了包括所公开的化合物和另外的组分的试剂盒，例如细胞，抗体，β-乳酸酶或在测定中使用组分的说明书。

2. 发明授权

US08318450B2 Optically-detectable enzyme substrates and their method of use 有权
标题翻译：光学上可检测的酶底物及其使用方法
公开(公告)号：US08318450B2
公开(公告)日：2012-11-27
申请号：US13166627
申请日：2011-06-22
申请人： Schuyler Corry , William Downey , Brian Filanoski , Kyle Gee , Lawrence Greenfield , James Hirsch , Iain Johnson , Aleksey Rukavishnikov
发明人： Schuyler Corry , William Downey , Brian Filanoski , Kyle Gee , Lawrence Greenfield , James Hirsch , Iain Johnson , Aleksey Rukavishnikov
IPC分类号： C12Q1/34
CPC分类号： C07F5/022 , C07D499/00 , C07D501/00 , C07D501/58 , C07D501/60 , C07D503/00 , C07D519/00 , C12Q1/34 , C12Q2334/00 , G01N33/581 , G01N2333/986
摘要： The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a β-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a β-lactmase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a β-lactmase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a β-lactmase or instructions for using the components in an assay.
摘要翻译：本发明涉及作为酶的底物的化合物，并且在与酶反应时提供可检测的反应，例如可光学检测的反应。特别地，这些化合物可用于检测样品中β-内酰胺酶的存在。除了化合物之外，公开了用于分析样品存在β-乳糖酶的方法，例如，作为包括编码β-乳糖酶的序列的核酸序列的表达的指示剂。公开了包括所公开的化合物和另外的组分的试剂盒，例如细胞，抗体，β-乳酸酶或在测定中使用组分的说明书。

3. 发明授权

US08865891B2 Optically-detectable enzyme substrates and their method of use 有权
标题翻译：光学上可检测的酶底物及其使用方法
公开(公告)号：US08865891B2
公开(公告)日：2014-10-21
申请号：US13614948
申请日：2012-09-13
申请人： Schuyler Corry , William Downey , Brian Filanoski , Kyle Gee , Lawrence Greenfield , James Hirsch , Iain Johnson , Aleksey Rukavishnikov
发明人： Schuyler Corry , William Downey , Brian Filanoski , Kyle Gee , Lawrence Greenfield , James Hirsch , Iain Johnson , Aleksey Rukavishnikov
IPC分类号： C07D501/36 , C07D501/58 , C07D501/00 , C07F5/02 , C07D503/00 , C07D499/00 , C12Q1/34
CPC分类号： C07F5/022 , C07D499/00 , C07D501/00 , C07D501/58 , C07D501/60 , C07D503/00 , C07D519/00 , C12Q1/34 , C12Q2334/00 , G01N33/581 , G01N2333/986
摘要： The present invention relates to compounds that are substrates for an enzyme, and upon reaction with the enzyme provide a detectable response, such as an optically detectable response. In particular, the compounds have utility in detecting the presence of a β-lactamase in a sample. In addition to the compounds, methods are disclosed for analyzing a sample for the presence of a β-lactamase, for example, as an indicator of expression of a nucleic acid sequence including a sequence coding for a β-lactamase. Kits are disclosed that include the disclosed compounds and additional components, for example, cells, antibodies, a β-lactamase or instructions for using the components in an assay.
摘要翻译：本发明涉及作为酶的底物的化合物，并且在与酶反应时提供可检测的反应，例如可光学检测的反应。特别地，这些化合物可用于检测样品中β-内酰胺酶的存在。除了化合物之外，公开了用于分析样品中β-内酰胺酶的存在的方法，例如，作为包括编码β-内酰胺酶的序列的核酸序列的表达的指示剂。公开了包括所公开的化合物和另外的组分的试剂盒，例如细胞，抗体，β-内酰胺酶或在测定中使用组分的说明书。

4. 发明申请

US20120077211A1 STABLE COMPOSITIONS COMPRISING CHROMOGENIC COMPOUNDS AND METHODS OF USE 审中-公开
标题翻译：包含色素化合物的稳定组合物和使用方法
公开(公告)号：US20120077211A1
公开(公告)日：2012-03-29
申请号：US13139873
申请日：2009-12-15
申请人： Lawrence Greenfield , Shawn Starkenburg , Matthew Shallice , Julie Nyhus , Louis Leong
发明人： Lawrence Greenfield , Shawn Starkenburg , Matthew Shallice , Julie Nyhus , Louis Leong
IPC分类号： G01N33/53 , C12Q1/28 , C09K3/00 , C07C211/54
CPC分类号： G01N33/532 , C01B15/037
摘要： Compositions, assays, methods, and kits are disclosed for use in applications that utilize oxidation of a chromogenic electron donor such as diaminobenzidine (DAB) to generate a signal. Applications include, but are not limited to, immunohistochemistry, chromogenic in situ hybridization, Western blots, Northern blots, Southern blots, ELISA assays, and microarray detection. The compositions, assays, methods, and kits disclosed herein make use of a novel, stabilized formulation of DAB and a novel, stabilized formulation of hydrogen peroxide.
摘要翻译：公开了组合物，测定法，方法和试剂盒，用于利用显色电子给体如二氨基联苯胺（DAB）氧化产生信号的应用中。应用包括但不限于免疫组化，显色原位杂交，Western印迹，Northern印迹，Southern印迹，ELISA测定和微阵列检测。本文公开的组合物，测定方法和试剂盒使用DAB的新型稳定制剂和新型稳定的过氧化氢制剂。

5. 发明授权

US07208271B2 Compositions and methods of selective nucleic acid isolation 有权
公开(公告)号：US07208271B2
公开(公告)日：2007-04-24
申请号：US10306347
申请日：2002-11-27
申请人： Douglas A. Bost , Lawrence Greenfield
发明人： Douglas A. Bost , Lawrence Greenfield
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04 , C07H21/00
CPC分类号： C12Q1/6806 , C12N15/1006 , C12N15/101
摘要： The invention relates to methods for isolating and/or identifying nucleic acids. The invention also provides kits for isolating and/or identifying nucleic acids.

6. 发明申请

US20060141523A1 Integrated method for PCR cleanup and oligonucleotide removal 审中-公开
标题翻译：用于PCR清除和寡核苷酸去除的综合方法
公开(公告)号：US20060141523A1
公开(公告)日：2006-06-29
申请号：US11357822
申请日：2006-02-17
申请人： Lawrence Greenfield , Douglas Bost
发明人： Lawrence Greenfield , Douglas Bost
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6848 , C12Q2525/197
摘要： A method is provided for purifying a desired polynucleotide product by removing unincorporated oligonucleotides from a polymerase or ligase reaction mixture.
摘要翻译：提供了通过从聚合酶或连接酶反应混合物中除去未掺入的寡核苷酸来纯化所需多核苷酸产物的方法。

7. 发明申请

US20050176076A1 Multiplex protease profiling 审中-公开
标题翻译：多重蛋白酶分析
公开(公告)号：US20050176076A1
公开(公告)日：2005-08-11
申请号：US11025608
申请日：2004-12-28
申请人： Hongye Sun , Lawrence Greenfield , Douglas Bost
发明人： Hongye Sun , Lawrence Greenfield , Douglas Bost
IPC分类号： C12Q1/37 , G01N33/53 , G01N33/537 , G01N33/543 , G01N33/574
CPC分类号： C12Q1/37
摘要： In certain embodiments, provided are methods and compositions useful for the detection and quantitation of catalytically active enzymes, particularly catalytically active proteases. The compositions comprise one or more multifunctional tags, wherein each different multifunctional tag comprises a different mobility modifier, a partitioner, a reporter, and a peptide substrate that is specifically or substantially specifically hydrolyzed by a target protease or target protease family. Hydrolysis of each different multifunctional tag by a target protease provides a different labeled hydrolytic product comprising a reporter and a mobility modifier but not a partitioner, where the mobility modifier confers a distinctive mobility on the labeled hydrolytic product in a mobility-dependent analysis technique.
摘要翻译：在某些实施方案中，提供了可用于检测和定量催化活性酶，特别是催化活性蛋白酶的方法和组合物。组合物包含一个或多个多功能标签，其中每个不同的多功能标签包含由靶蛋白酶或靶蛋白酶家族特异性或基本上特异性水解的不同的迁移率调节剂，分隔器，报道分子和肽底物。通过靶蛋白酶水解每种不同的多功能标签物提供了不同的标记的水解产物，其包含报道分子和迁移率调节剂，而不是分配器，其中迁移率调节剂在迁移依赖性分析技术中赋予标记的水解产物上独特的迁移率。

8. 发明授权

US10198521B2 Processing ambiguous search requests in a geographic information system 有权
公开(公告)号：US10198521B2
公开(公告)日：2019-02-05
申请号：US11426760
申请日：2006-06-27
申请人： Lawrence Greenfield , Daniel Egnor , Francois Bailly , John Hanke
发明人： Lawrence Greenfield , Daniel Egnor , Francois Bailly , John Hanke
IPC分类号： G06F17/30 , G06F3/0481 , G06T17/05 , G06N7/00 , G06Q40/00
摘要： Interactive geographic information systems (GIS) and techniques are disclosed that provide users with a greater degree of flexibility, utility, and information. A markup language is provided that facilitates communication between servers and clients of the interactive GIS, which enables a number of GIS features, such as network links (time-based and/or view-dependent dynamic data layers), ground overlays, screen overlays, placemarks, 3D models, and stylized GIS elements, such as geometry, icons, description balloons, polygons, and labels in the viewer by which the user sees the target area. Also, “virtual tours” of user-defined paths in the context of distributed geospatial visualization is enabled. Streaming and interactive visualization of filled polygon data are also enabled thereby allowing buildings and other such features to be provided in 3D. Also, techniques for enabling ambiguous search requests in a GIS are provided.

9. 发明授权

US10001474B2 Fluorogenic semiconductor nanocrystals 有权
公开(公告)号：US10001474B2
公开(公告)日：2018-06-19
申请号：US14124354
申请日：2012-06-07
申请人： Eric Welch , David Bussian , Michael Ignatius , Lawrence Greenfield
发明人： Eric Welch , David Bussian , Michael Ignatius , Lawrence Greenfield
IPC分类号： G01N33/52 , G01N33/58 , C09K11/08 , B82Y15/00
CPC分类号： G01N33/525 , B82Y15/00 , C09K11/08 , G01N33/581 , G01N33/588
摘要： Fluorogenic semiconductor nanocrystals and compositions thereof are provided herein, including kits, assay systems and methods for their preparation and use.

10. 发明授权

US07537898B2 Compositions and methods of selective nucleic acid isolation 有权
标题翻译：选择性核酸分离的组合物和方法
公开(公告)号：US07537898B2
公开(公告)日：2009-05-26
申请号：US11789352
申请日：2007-04-23
申请人： Douglas A. Bost , Lawrence Greenfield
发明人： Douglas A. Bost , Lawrence Greenfield
IPC分类号： C12Q1/68 , C07H21/00 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6806 , C12N15/1006 , C12N15/101
摘要： The invention relates to methods for isolating and/or identifying nucleic acids. The invention also provides kits for isolating and/or identifying nucleic acids.
摘要翻译：本发明涉及用于分离和/或鉴定核酸的方法。本发明还提供用于分离和/或鉴定核酸的试剂盒。

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