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1. 发明申请

US20070264695A1 Method for producing a purified lipase 审中-公开
标题翻译：纯化脂肪酶的制备方法
公开(公告)号：US20070264695A1
公开(公告)日：2007-11-15
申请号：US11826459
申请日：2007-07-16
申请人： Satoshi Negishi , Junko Suzuki , Chika Sakurai , Shin Arimoto , Hidetaka Uehara , Tadashiro Hirose , Yoshie Yamauchi , Yuri Arai , Tomomi Suganuma
发明人： Satoshi Negishi , Junko Suzuki , Chika Sakurai , Shin Arimoto , Hidetaka Uehara , Tadashiro Hirose , Yoshie Yamauchi , Yuri Arai , Tomomi Suganuma
IPC分类号： C12P7/64 , C12N9/20
CPC分类号： C12N9/18
摘要： The present invention provides a method for producing a purified lipase which comprises steps of: (a) bringing a long chain fatty acid triglyceride and a medium-chain triglyceride into contact with a lipase to purify the lipase; and (b) collecting the purified lipase. According to this production method, the purified lipase can be obtained wherein content of impurity such as silicon is decreased.
摘要翻译：本发明提供了一种纯化脂肪酶的制备方法，其特征在于，包括以下步骤：（a）使长链脂肪酸甘油三酯和中链甘油三酯与脂肪酶接触以纯化脂肪酶; 和（b）收集纯化的脂肪酶。根据该制造方法，可以得到其中杂质如硅的含量降低的纯化的脂肪酶。

2. 发明申请

US20060105438A1 1,3-Specific lipase powder, methods for producing the same and use thereof 审中-公开
标题翻译： 1,3-特异性脂肪酶粉末，其制造方法及其用途
公开(公告)号：US20060105438A1
公开(公告)日：2006-05-18
申请号：US11320760
申请日：2005-12-30
申请人： Junko Suzuki , Satoshi Negishi , Yuri Arai , Chika Sakurai , Tadashiro Hirose , Hidetaka Uehara , Shin Arimoto , Tomomi Suganuma
发明人： Junko Suzuki , Satoshi Negishi , Yuri Arai , Chika Sakurai , Tadashiro Hirose , Hidetaka Uehara , Shin Arimoto , Tomomi Suganuma
IPC分类号： C12P7/64
CPC分类号： C12P7/6454 , C12N9/20
摘要： A method for producing a lipase powder, which comprises the step of spray drying an aqueous solution containing a lipase adjusted to pH 6 to 7.5; the lipase powder which is a 1,3-specific lipase derived from Rhizomucor miehei, spherical, and contains not more than 10% by weight of moisture; and a lipase composition wherein the lipase powder is immersed or impregnated in fatty oil. According to the present invention, a lipase powder of which 1,3-selectivity is improved can be provided.
摘要翻译：一种生产脂肪酶粉末的方法，其包括将含有调节至pH6-7.5的脂肪酶的水溶液喷雾干燥的步骤; 所述脂肪酶粉末是来自根毛霉（Rhizomucor miehei）的1,3-特异性脂肪酶，为球形，并且含有不超过10重量％的水分; 脂肪酶组合物，其中将脂肪酶粉末浸渍或浸渍在脂肪油中。根据本发明，可以提供改善1,3-选择性的脂肪酶粉末。

3. 发明授权

US08980346B2 Process for preparing hard butter 有权
标题翻译：硬脂黄油的制备方法
公开(公告)号：US08980346B2
公开(公告)日：2015-03-17
申请号：US12676791
申请日：2008-09-08
申请人： Shin Arimoto , Hidetaka Uehara , Tomomi Suganuma , Kinya Tsuchiya , Satoshi Negishi
发明人： Shin Arimoto , Hidetaka Uehara , Tomomi Suganuma , Kinya Tsuchiya , Satoshi Negishi
IPC分类号： A23D9/00 , C11C3/10 , A21D2/16 , A23G1/36 , C11C1/10 , C11C3/12
CPC分类号： C11C3/10 , A21D2/165 , A23D9/00 , A23G1/36 , C11C1/10 , C11C3/12
摘要： The present invention relates to a process for preparing a hard butter, which comprises conducting transesterification between one or more selected from the group consisting of straight-chain saturated C16-22 fatty acids and lower alcohol esters thereof and a triglyceride having oleoyl group and/or linoleoyl group at the 2-position with an action of a granulated powder lipase comprising a lipase derived from Rhizopus oryzae and/or Rhizopus delemar and a soybean powder, and removing the granulated powder lipase after the transesterification. The process is industrially suitable process for preparing a hard butter, which have excellent properties as a cacao butter equivalent.
摘要翻译：本发明涉及一种制备硬脂黄油的方法，该方法包括进行一种或多种选自直链饱和C16-22脂肪酸和其低级醇酯和含有油酰基的甘油三酯的酯交换和/或亚油酰基在2位上具有造粒粉末脂肪酶的作用，其包含源自米曲霉和/或根霉的大豆粉末的脂肪酶，并且在酯交换后除去造粒的粉末脂肪酶。该工艺是工业上合适的制备硬质奶油的方法，其具有作为可可脂当量的优异性能。

4. 发明授权

US08389754B2 Fractionation method of 1,3-disaturated-2-unsaturated triglyceride 有权
标题翻译： 1,3-二饱和-2-不饱和甘油三酯的分馏方法
公开(公告)号：US08389754B2
公开(公告)日：2013-03-05
申请号：US12676809
申请日：2008-09-08
申请人： Shin Arimoto , Hidetaka Uehara , Tomomi Suganuma , Kinya Tsuchiya , Satoshi Negishi
发明人： Shin Arimoto , Hidetaka Uehara , Tomomi Suganuma , Kinya Tsuchiya , Satoshi Negishi
IPC分类号： C07C51/43
CPC分类号： C11B7/0075 , C11B7/0025 , C11C1/10 , C11C3/10
摘要： The present invention discloses a method of producing triglycerides rich in XOX fat and/or XLX fat, which comprises the steps of heating and dissolving triglycerides (XOX fat and/or XLX fat) which comprise 20 to 60 mass % of a triglyceride having a saturated fatty acid residue on each of the first and third position and an oleoyl group and/or a linoleoyl group on the second position in total triglycerides in the presence of 1 to 30 mass % of a fatty acid lower alkyl ester; and then cooling the mixture to precipitate crystals and conducting solid-liquid separation. This method is a more efficient and industrially suitable fractionation and production method of fats and oils which are rich in a triglyceride (XOX fat and/or XLX fat) having a saturated fatty acid residue on each of the first and third position and an oleoyl group and/or a linoleoyl group on the second position.
摘要翻译：本发明公开了一种生产富含XOX脂肪和/或XLX脂肪的甘油三酸酯的方法，其包括加热和溶解含有20〜60质量％甘油三酯的甘油三酯（XOX脂肪和/或XLX脂肪）的步骤，在1至30质量％的脂肪酸低级烷基酯存在下，在总甘油三酸酯中的第一和第三位上的脂肪酸残基和第二位置上的油酰基和/或亚油酰基; 然后冷却混合物以沉淀晶体并进行固液分离。该方法是富含甘油三酯（XOX脂肪和/或XLX脂肪）的脂肪和油的更有效和工业上合适的分馏和制备方法，其在第一和第三位置上具有饱和脂肪酸残基和油酰基和/或亚油酰基在第二位置。

5. 发明申请

US20100255152A1 PROCESS FOR PREPARING HARD BUTTER 有权
标题翻译：制备硬切片的方法
公开(公告)号：US20100255152A1
公开(公告)日：2010-10-07
申请号：US12676791
申请日：2008-09-08
申请人： Shin Arimoto , Hidetaka Uehara , Tomomi Suganuma , Kinya Tsuchiya , Satoshi Negishi
发明人： Shin Arimoto , Hidetaka Uehara , Tomomi Suganuma , Kinya Tsuchiya , Satoshi Negishi
IPC分类号： A23D9/02
CPC分类号： C11C3/10 , A21D2/165 , A23D9/00 , A23G1/36 , C11C1/10 , C11C3/12
摘要： The present invention relates to a process for preparing a hard butter, which comprises conducting transesterification between one or more selected from the group consisting of straight-chain saturated C16-22 fatty acids and lower alcohol esters thereof and a triglyceride having oleoyl group and/or linoleoyl group at the 2-position with an action of a granulated powder lipase comprising a lipase derived from Rhizopus oryzae and/or Rhizopus delemar and a soybean powder, and removing the granulated powder lipase after the transesterification. The process is industrially suitable process for preparing a hard butter, which have excellent properties as a cacao butter equivalent.
摘要翻译：本发明涉及一种制备硬脂黄油的方法，该方法包括进行一种或多种选自直链饱和C16-22脂肪酸和其低级醇酯和含有油酰基的甘油三酯的酯交换和/或亚油酰基在2位上具有造粒粉末脂肪酶的作用，其包含源自米曲霉和/或根霉的大豆粉末的脂肪酶，并且在酯交换后除去造粒的粉末脂肪酶。该工艺是工业上合适的制备硬质奶油的方法，其具有作为可可脂当量的优异性能。

6. 发明申请

US20100222607A1 FRACTIONATION METHOD OF 1,3-DISATURATED-2-UNSATURATED TRIGLYCERIDE 有权
标题翻译： 1,3-二饱和二不饱和化合物的分解方法
公开(公告)号：US20100222607A1
公开(公告)日：2010-09-02
申请号：US12676809
申请日：2008-09-08
申请人： Shin Arimoto , Hidetaka Uehara , Tomomi Suganuma , Kinya Tsuchiya , Satoshi Negishi
发明人： Shin Arimoto , Hidetaka Uehara , Tomomi Suganuma , Kinya Tsuchiya , Satoshi Negishi
IPC分类号： C11B7/00
CPC分类号： C11B7/0075 , C11B7/0025 , C11C1/10 , C11C3/10
摘要： The present invention discloses a method of producing triglycerides rich in XOX fat and/or XLX fat, which comprises the steps of heating and dissolving triglycerides (XOX fat and/or XLX fat) which comprise 20 to 60 mass % of a triglyceride having a saturated fatty acid residue on each of the first and third position and an oleoyl group and/or a linoleoyl group on the second position in total triglycerides in the presence of 1 to 30 mass % of a fatty acid lower alkyl ester; and then cooling the mixture to precipitate crystals and conducting solid-liquid separation. This method is a more efficient and industrially suitable fractionation and production method of fats and oils which are rich in a triglyceride (XOX fat and/or XLX fat) having a saturated fatty acid residue on each of the first and third position and an oleoyl group and/or a linoleoyl group on the second position.
摘要翻译：本发明公开了一种生产富含XOX脂肪和/或XLX脂肪的甘油三酸酯的方法，其包括加热和溶解含有20〜60质量％甘油三酯的甘油三酯（XOX脂肪和/或XLX脂肪）的步骤，在1至30质量％的脂肪酸低级烷基酯存在下，在总甘油三酸酯中的第一和第三位上的脂肪酸残基和第二位置上的油酰基和/或亚油酰基; 然后冷却混合物以沉淀晶体并进行固液分离。该方法是富含甘油三酯（XOX脂肪和/或XLX脂肪）的脂肪和油的更有效和工业上合适的分馏和制备方法，其在第一和第三位置上具有饱和脂肪酸残基和油酰基和/或亚油酰基在第二位置。

7. 发明授权

US07442531B2 Method for producing symmetric triglycerides 有权
标题翻译：生产对称甘油三酯的方法
公开(公告)号：US07442531B2
公开(公告)日：2008-10-28
申请号：US11326436
申请日：2006-01-06
申请人： Satoshi Negishi , Yuri Arai , Shin Arimoto , Hidetaka Uehara
发明人： Satoshi Negishi , Yuri Arai , Shin Arimoto , Hidetaka Uehara
IPC分类号： C12P7/64
CPC分类号： A23D9/00 , C11C3/10 , C12P7/6454 , C12P7/6472
摘要： A method for producing symmetric triglycerides, which comprises the steps of reacting a medium chain fatty acid triglyceride with a long chain fatty acid triglyceride in the presence of an enzyme or a chemical catalyst to conduct random transesterification reaction and thereby to obtain a reaction product containing a triglyceride composed of a medium chain fatty acid and a long chain fatty acid as the constituting fatty acids in the step of the first reaction; transesterifying the reaction product with an alcohol monoester of the medium chain fatty acid in the presence of an sn-1,3-position specific enzyme in the step of the second reaction and then taking the alcohol monoester of the medium chain fatty acid and the alcohol monoester of the long chain fatty acid from the reaction product obtained in the step of the second reaction to obtain the symmetric triglyceride composed of the medium chain fatty acids at the sn-1, 3 positions and the long chain fatty acid at the sn-2 position. According to this method, highly pure symmetric triglycerides comprising a medium-chain fatty acid at sn-1 and 3 positions and a long chain fatty acid at sn-2 position can be industrially efficiently produced.
摘要翻译：一种对称甘油三酯的制造方法，其特征在于，在酶或化学催化剂存在下使中链脂肪酸甘油三酯与长链脂肪酸甘油三酯反应，进行无规酯交换反应，得到含有在第一反应步骤中由构成脂肪酸的中链脂肪酸和长链脂肪酸组成的甘油三酸酯; 在第二反应步骤中，在sn-1,3位特异性酶存在下，用中链脂肪酸的醇单酯将反应产物进行酯交换，然后取中链脂肪酸和醇的醇单酯在第二反应步骤中获得的反应产物中的长链脂肪酸的单酯，得到由sn-1,3位置的中链脂肪酸组成的对称甘油三酯，sn-2处的长链脂肪酸位置。根据该方法，可以在工业上有效地制造包含sn-1和3位的中链脂肪酸和sn-2位的长链脂肪酸的高纯度对称甘油三酯。

8. 发明申请

US20060115882A1 Method for producing symmetric triglycerides 有权
标题翻译：生产对称甘油三酯的方法
公开(公告)号：US20060115882A1
公开(公告)日：2006-06-01
申请号：US11326436
申请日：2006-01-06
申请人： Satoshi Negishi , Yuri Arai , Shin Arimoto , Hidetaka Uehara
发明人： Satoshi Negishi , Yuri Arai , Shin Arimoto , Hidetaka Uehara
IPC分类号： C12P7/64
CPC分类号： A23D9/00 , C11C3/10 , C12P7/6454 , C12P7/6472
摘要： A method for producing symmetric triglycerides, which comprises the steps of reacting a medium chain fatty acid triglyceride with a long chain fatty acid triglyceride in the presence of an enzyme or a chemical catalyst to conduct random transesterification reaction and thereby to obtain a reaction product containing a triglyceride composed of a medium chain fatty acid and a long chain fatty acid as the constituting fatty acids in the step of the first reaction; transesterifying the reaction product with an alcohol monoester of the medium chain fatty acid in the presence of an sn-1,3-position specific enzyme in the step of the second reaction and then taking the alcohol monoester of the medium chain fatty acid and the alcohol monoester of the long chain fatty acid from the reaction product obtained in the step of the second reaction to obtain the symmetric triglyceride composed of the medium chain fatty acids at the sn-1, 3 positions and the long chain fatty acid at the sn-2 position. According to this method, highly pure symmetric triglycerides comprising a medium-chain fatty acid at sn-1 and 3 positions and a long chain fatty acid at sn-2 position can be industrially efficiently produced.
摘要翻译：一种对称甘油三酯的制造方法，其特征在于，在酶或化学催化剂存在下使中链脂肪酸甘油三酯与长链脂肪酸甘油三酯反应，进行无规酯交换反应，得到含有在第一反应步骤中由构成脂肪酸的中链脂肪酸和长链脂肪酸组成的甘油三酸酯; 在第二反应步骤中，在sn-1,3位特异性酶存在下，用中链脂肪酸的醇单酯将反应产物进行酯交换，然后取中链脂肪酸和醇的醇单酯在第二反应步骤中获得的反应产物中的长链脂肪酸的单酯，得到由sn-1,3位置的中链脂肪酸组成的对称甘油三酯，sn-2处的长链脂肪酸位置。根据该方法，可以在工业上有效地制造包含sn-1和3位的中链脂肪酸和sn-2位的长链脂肪酸的高纯度对称甘油三酯。

9. 发明授权

US07423163B2 Process for producing concentrate of conjugated fatty acid 失效
标题翻译：生产共轭脂肪酸浓缩物的方法
公开(公告)号：US07423163B2
公开(公告)日：2008-09-09
申请号：US11902349
申请日：2007-09-20
申请人： Hidetaka Uehara , Tomomi Suganuma , Satoshi Negishi , Toshihisa Suzuki
发明人： Hidetaka Uehara , Tomomi Suganuma , Satoshi Negishi , Toshihisa Suzuki
IPC分类号： C11C3/14 , C11B3/02
CPC分类号： C11C1/007 , A23V2002/00 , C11C1/005 , C11C3/02 , A23V2250/1866
摘要： An easy and inexpensive process by which a concentrate of a given unsaturated fatty acid can be obtained from a mixture which has conventionally been difficult to concentrate. The process, which is for producing a concentrate of a desired isomer (a) from a conjugated fatty acid mixture (A), is characterized by comprising: a step in which the conjugated fatty acid mixture (A) is mixed with at least one unsaturated fatty acid (B) to obtain a mixture solution containing the isomer (a) dissolved therein; a crystallization step in which either crystals rich in the isomer (a) or crystals poor in the isomer (a) are precipitated from the mixture solution; and a solid-liquid separation step for obtaining the crystals rich in the isomer (a) or for obtaining a solution rich in the isomer (a) by removing the crystals poor in the isomer (a).
摘要翻译：一种容易且廉价的方法，通过该方法可以从常规难以浓缩的混合物中获得给定的不饱和脂肪酸的浓缩物。用于由共轭脂肪酸混合物（A）生产所需异构体（a）的浓缩物的方法的特征在于包括：将共轭脂肪酸混合物（A）与至少一种不饱和脂肪酸混合物脂肪酸（B），得到含有溶解于其中的异构体（a）的混合溶液; 从混合溶液中析出析出富含异构体（a）或异构体（a）的晶体不足的晶体的结晶步骤; 以及固体 - 液体分离步骤，用于通过除去异构体（a）中的不稳定的晶体来获得富含异构体（a）的晶体或用于获得富含异构体（a）的溶液。

10. 发明申请

US20080027238A1 Process for producing concentrate of conjugated fatty acid 失效
公开(公告)号：US20080027238A1
公开(公告)日：2008-01-31
申请号：US11902349
申请日：2007-09-20
申请人： Hidetaka Uehara , Tomomi Suganuma , Satoshi Negishi , Toshihisa Suzuki
发明人： Hidetaka Uehara , Tomomi Suganuma , Satoshi Negishi , Toshihisa Suzuki
IPC分类号： C07C51/347
CPC分类号： C11C1/007 , A23V2002/00 , C11C1/005 , C11C3/02 , A23V2250/1866
摘要： An easy and inexpensive process by which a concentrate of a given unsaturated fatty acid can be obtained from a mixture which has conventionally been difficult to concentrate. The process, which is for producing a concentrate of a desired isomer (a) from a conjugated fatty acid mixture (A), is characterized by comprising: a step in which the conjugated fatty acid mixture (A) is mixed with at least one unsaturated fatty acid (B) to obtain a mixture solution containing the isomer (a) dissolved therein; a crystallization step in which either crystals rich in the isomer (a) or crystals poor in the isomer (a) are precipitated from the mixture solution; and a solid-liquid separation step for obtaining the crystals rich in the isomer (a) or for obtaining a solution rich in the isomer (a) by removing the crystals poor in the isomer (a).

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