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1. 发明授权

US10174331B2 Methods and compositions for nuclease-mediated targeted integration of transgenes 有权
公开(公告)号：US10174331B2
公开(公告)日：2019-01-08
申请号：US13889162
申请日：2013-05-07
申请人： Sangamo BioSciences, Inc. , Dow AgroSciences LLC
发明人： Gregory J. Cost , Fyodor Urnov , W. Michael Ainley , Joseph F. Petolino , Jayakumar Pon Samuel , Steven R. Webb , Lakshmi Sastry-Dent
IPC分类号： C12N15/82 , C12N15/87 , C12N15/85 , C12N15/10
摘要： Disclosed herein are methods and compositions for homology-independent targeted insertion of donor molecules into the genome of a cell.

2. 发明申请

US20130326645A1 METHODS AND COMPOSITIONS FOR NUCLEASE-MEDIATED TARGETED INTEGRATION OF TRANSGENES 审中-公开
标题翻译：用于核转移介导的靶向转移整合的方法和组合物
公开(公告)号：US20130326645A1
公开(公告)日：2013-12-05
申请号：US13889162
申请日：2013-05-07
申请人： Dow AgroSciences LLC , Sangamo BioSciences, Inc.
发明人： Gregory J. Cost , Fyodor Urnov , William M. Ainley , Joseph F. Petolino , Jayakumar Pon Samuel , Steven R. Webb , Lakshmi Sastry-Dent
IPC分类号： C12N15/85 , C12N15/82
CPC分类号： C12N15/8201 , C12N15/102 , C12N15/8209 , C12N15/8213 , C12N15/8216 , C12N15/8241 , C12N15/8247 , C12N15/8274 , C12N15/8278 , C12N15/8286 , C12N15/85 , C12N15/87 , C12N2800/80 , Y02A40/162
摘要： Disclosed herein are methods and compositions for homology-independent targeted insertion of donor molecules into the genome of a cell.
摘要翻译：本文公开了用于与供体分子同源性独立的靶向插入细胞基因组的方法和组合物。

3. 发明申请

US20140242703A1 METHODS FOR TRANSFERRING MOLECULAR SUBSTANCES INTO PLANT CELLS 有权
标题翻译：将分子物质转移到植物细胞中的方法
公开(公告)号：US20140242703A1
公开(公告)日：2014-08-28
申请号：US14275613
申请日：2014-05-12
申请人： Dow AgroSciences LLC
发明人： Jayakumar Pon Samuel , Frank G. Burroughs , Suraj K. Dixit , Mark W. Zettler
IPC分类号： C12N15/82
CPC分类号： C12N15/8207 , C12N15/8206
摘要： Provided are methods for introducing a molecule of interest into a plant cell comprising a cell wall. Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.
摘要翻译：提供了将感兴趣的分子引入包含细胞壁的植物细胞中的方法。提供用于遗传或其他修饰植物和用于治疗或预防包含细胞壁的植物细胞中的疾病的方法。

4. 发明授权

US09719108B2 Nanoparticle mediated delivery of sequence specific nucleases 有权
公开(公告)号：US09719108B2
公开(公告)日：2017-08-01
申请号：US14861864
申请日：2015-09-22
申请人： Dow AgroSciences LLC
发明人： Jayakumar Pon Samuel , Joseph F. Petolino , Narasimha Chary Samboju , Steven R. Webb , Kerrm Y. Yau
IPC分类号： C12N15/90 , C12N15/82 , C12N5/04 , C12N9/22 , C12N9/96
CPC分类号： C12N15/902 , C12N5/04 , C12N9/22 , C12N9/96 , C12N15/8206 , C12Y301/21004
摘要： Provided are methods for introducing a sequence-specific nuclease into a plant cell comprising a cell wall. Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.

5. 发明授权

US10167483B2 Herbicide resistance genes 有权
公开(公告)号：US10167483B2
公开(公告)日：2019-01-01
申请号：US14491197
申请日：2014-09-19
申请人： Dow AgroSciences LLC
发明人： Terry R. Wright , Justin M. Lira , Terence Anthony Walsh , Donald Merlo , Jayakumar Pon Samuel , Gaofeng Lin
IPC分类号： C12N15/82 , C12N9/02
摘要： The subject invention provides novel plants that are not only resistant to 2,4-D, but also to pyridyloxyacetate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention “stacked” together with one or more other herbicide resistance genes. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the development of, and controlling, strains of weeds that are resistant to one or more herbicides such as glyphosate. The preferred enzyme and gene for use according to the subject invention are referred to herein as AAD-12 (AryloxyAlkanoate Dioxygenase). This highly novel discovery is the basis of significant herbicide tolerant crop trait and selectable marker opportunities.

6. 发明申请

US20140283225A1 EARLY DETECTION AND ELIMINATION OF NON GERM-LINE EVENTS IN THE SOYBEAN TRANSFORMATION PROCESS 审中-公开
标题翻译：在大豆转化过程中早期检测和消除非线性事件
公开(公告)号：US20140283225A1
公开(公告)日：2014-09-18
申请号：US14211091
申请日：2014-03-14
申请人： Dow Agrosciences LLC
发明人： Jayakumar Pon Samuel , Sivarama Reddy Chennareddy , Andy Bowling , John Whitteck , Tatyana Minnicks , Dayakar Pareddy , Kerrm Y. Yau , Kelley A. Smith
IPC分类号： G01N33/569
CPC分类号： C12N15/821
摘要： The present disclosure relates in part to a method for identifying a soybean germline transformant from a population of soybean transformants which are comprised of a combination of soybean non-germline transformants and soybean germline transformants. The soybean non-germline transformants are identified and eliminated early in the transformation process. The soybean germline transformants are detected and selected for culturing into mature soybean plants. The method is readily applicable for screening and obtaining a soybean germline transformant at an early stage in the transformation process.
摘要翻译：本公开部分地涉及从大豆非种系转化体和大豆种系转化体的组合组成的大豆转化体群体中鉴定大豆种系转化体的方法。大豆非种系转化体在转化过程的早期被鉴定和消除。检测并选择大豆种系转化体用于培养成熟大豆植物。该方法易于在转化过程的早期筛选和获得大豆种系转化体。

7. 发明授权

US10214746B2 Soybean transformation method 有权
公开(公告)号：US10214746B2
公开(公告)日：2019-02-26
申请号：US14505060
申请日：2014-10-02
申请人： Dow AgroSciences LLC
发明人： Sivarama Reddy Chennareddy , Dayakar Pareddy , Jayakumar Pon Samuel , Rodrigo Sarria-Milan , Toby Cicak
IPC分类号： C12N15/82
摘要： The present disclosure relates in part to a method for identifying a soybean germline transformant from a population of soybean transformants by incorporating a selection agent within rooting medium used in tissue culture during the soybean transformation process. The soybean germline transformants are selected from a population of soybean transformants which are comprised of a combination of non-germline and germline soybean transformants. The soybean non-germline transformants are identified and eliminated early in the transformation process. The soybean germline transformants are identified and selected for culturing into mature soybean plants. The method is readily applicable for screening and obtaining a soybean germline transformant at an early stage in the soybean transformation process.

8. 发明授权

US09476057B2 Methods for transferring molecular substances into plant cells 有权
标题翻译：将分子物质转移到植物细胞中的方法
公开(公告)号：US09476057B2
公开(公告)日：2016-10-25
申请号：US14275613
申请日：2014-05-12
申请人： Dow AgroSciences LLC
发明人： Jayakumar Pon Samuel , Frank G. Burroughs , Suraj K. Dixit , Mark W. Zettler
IPC分类号： C12N15/82 , C12N15/87
CPC分类号： C12N15/8207 , C12N15/8206
摘要： Provided are methods for introducing a molecule of interest into a plant cell comprising a cell wall. Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.
摘要翻译：提供了将感兴趣的分子引入包含细胞壁的植物细胞中的方法。提供用于遗传或其他修饰植物和用于治疗或预防包含细胞壁的植物细胞中的疾病的方法。

9. 发明申请

US20150307889A1 HAPLOID MAIZE TRANSFORMATION 审中-公开
标题翻译： HAPLOID MAIZE转换
公开(公告)号：US20150307889A1
公开(公告)日：2015-10-29
申请号：US14695434
申请日：2015-04-24
申请人： Dow AgroSciences LLC
发明人： Joseph F. Petolino , Tonya L. Strange , Jayakumar Pon Samuel , Ryan C. Blue , Matthew A. Simpson
IPC分类号： C12N15/82
CPC分类号： C12N15/8213
摘要： Disclosed are methods for transformation of an androgenic-derived, haploid cell line with a site-specific nuclease. In some embodiments, the androgenic-derived, haploid cell line is a maize microspore-derived plant tissue culture. In addition, the disclosure provides a method for modifying, e.g., by mutating or targeting and integrating donor DNA into, a specific locus of a haploid or dihaploid tissue genome. The disclosure further provides methods for regenerating a whole plant from the haploid or dihaploid tissue that contains either the mutation at a specific genomic locus or a donor DNA integrated within a specific genomic locus may be obtained from the subject disclosure.
摘要翻译：公开了用位点特异性核酸酶转化雄激素衍生的单倍体细胞系的方法。在一些实施方案中，雄激素衍生的单倍体细胞系是来自玉米小孢子的植物组织培养物。此外，本公开提供了修饰，例如通过突变或靶向并将供体DNA整合到单倍体或双倍体组织基因组的特定基因座中的方法。本公开进一步提供了从单倍体或双倍体组织再生整个植物的方法，所述单体或双倍体组织含有特定基因组座位上的突变或整合在特定基因组基因组中的供体DNA，可从本发明公开内容获得。

10. 发明申请

US20170306339A1 METHODS FOR TRANSFERRING MOLECULAR SUBSTANCES INTO PLANT CELLS 审中-公开
公开(公告)号：US20170306339A1
公开(公告)日：2017-10-26
申请号：US15630568
申请日：2017-06-22
申请人： Dow AgroSciences LLC
发明人： Jayakumar Pon Samuel , Frank G. Burroughs , Suraj K. Dixit , Mark W. Zettler
IPC分类号： C12N15/82
CPC分类号： C12N15/8207 , C12N15/8206
摘要： Provided are methods for introducing a molecule of interest into a plant cell comprising a cell wall. Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.

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