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1. 发明申请

US20050191746A1 Compositions and methods for biodegradable polymer-peptide mediated transfection 失效
标题翻译：可生物降解聚合物 - 肽介导的转染的组合物和方法
公开(公告)号：US20050191746A1
公开(公告)日：2005-09-01
申请号：US10789589
申请日：2004-02-27
申请人： Sang Van , Nitnara Viroonchatapan , Shouping Ji , Kenji Matsumoto , Lei Yu
发明人： Sang Van , Nitnara Viroonchatapan , Shouping Ji , Kenji Matsumoto , Lei Yu
IPC分类号： A61K47/48 , A61K48/00 , C08L59/00 , C12N15/87 , C12N15/85 , C08G63/48
CPC分类号： C08L59/00 , A61K47/59 , A61K47/645 , A61K48/00 , C12N15/87
摘要： Complexes are disclosed which are a combination of a polynucleotide and a biodegradable polyacetal-peptide. The disclosed complexes are useful for cell transfection.
摘要翻译：公开了多核苷酸和生物可降解聚缩醛肽的组合的复合物。所公开的复合物可用于细胞转染。

2. 发明授权

US07446099B2 Compositions and methods for biodegradable polymer-peptide mediated transfection 失效
标题翻译：可生物降解聚合物 - 肽介导的转染的组合物和方法
公开(公告)号：US07446099B2
公开(公告)日：2008-11-04
申请号：US10789589
申请日：2004-02-27
申请人： Sang Van , Nitnara Viroonchatapan , Shouping Ji , Kenji Matsumoto , Lei Yu
发明人： Sang Van , Nitnara Viroonchatapan , Shouping Ji , Kenji Matsumoto , Lei Yu
IPC分类号： A01N43/48
CPC分类号： C08L59/00 , A61K47/59 , A61K47/645 , A61K48/00 , C12N15/87
摘要： Complexes are disclosed which are a combination of a polynucleotide and a biodegradable polyacetal-peptide. The disclosed complexes are useful for cell transfection.
摘要翻译：公开了多核苷酸和生物可降解聚缩醛肽的组合的复合物。所公开的复合物可用于细胞转染。

3. 发明授权

US07048925B2 Acid-sensitive polyacetals and methods 失效
标题翻译：酸敏聚缩醛和方法
公开(公告)号：US07048925B2
公开(公告)日：2006-05-23
申请号：US10651394
申请日：2003-08-28
申请人： Sang Van , Nitnara Viroonchatapan , Shouping Ji , Lei Yu
发明人： Sang Van , Nitnara Viroonchatapan , Shouping Ji , Lei Yu
IPC分类号： A61K39/44 , C08F283/00
CPC分类号： C08G4/00 , C07C229/22 , C08G73/028
摘要： Polyacetals comprising a recurring units represented by a formula selected from the group consisting of formula (I) and (II): wherein A comprises at least one acetal group; B is selected from the group consisting of —CH—, —CH(CH3)—, CH2CH—, —CH2C(CH3)—, —CH(CH3)CH—, and —CHCH(CH3)CH(CH3)—; Z is selected from the group consisting of C(O)OR1, C(O)SR1, C(O)NR1R2, and VU; V is a linker group; U is selected from the group consisting of poly(ethyleneimine), poly(propyleneimine), poly(lysine), PAMAM dendrimer, octaamine dendrimer, hexadecaamine dendrimer, enhancer, and targeting receptor; R1 and R2 are each individually selected from the group consisting of hydrogen, C1 to C10 alkyl, and C6 to C10 aryl; D is a linkage selected from the group consisting of carboxylic amide, carboxylic ester, urea, and urethane; and G is selected from the group consisting of C4 to C20 alkyl, C6-C10 aryl, and —(OCH2CH2)n—, where n is in the range of 1 to about 250, are useful in nucleic acid delivery applications. In preferred embodiments, complexes formed between polyacetals of the formulae (I) or (II) and polynucleotides are useful as transfection reagents.
摘要翻译：包含由选自式（I）和（II）的式所表示的重复单元的聚缩醛：其中A包含至少一个缩醛基; B选自-CH-，-CH（CH 3 CH 3） - ，CH 2 CH-，-CH 2 C （CH 3） - ， - CH（CH 3 CH 3）CH - 和-CHCH（CH 3 CH 3）CH（CH 3）） - ; Z选自C（O）OR 1，C（O）SR 1，C（O）NR 1 R ^{2和VU; V是连接基团; U选自聚（亚乙基亚胺），聚（丙烯亚胺），聚（赖氨酸），PAMAM树枝状聚合物，八胺树枝状聚合物，十六胺树枝状聚合物，增强剂和靶向受体; R 1和R 2各自各自独立地选自氢，C 1至C 10烷基和C 6至C 10芳基; D是选自羧酸酰胺，羧酸酯，脲和氨基甲酸酯的连接体; 并且G选自C 4〜C 20烷基，C 6 -C 10芳基，C 6〜和 - （OCH 2 CH 2 CH 2）n - ，其中n在1至约250的范围内，可用于核酸交付应用程序。在优选的实施方案中，在式（I）或（II）的聚缩醛和多核苷酸之间形成的络合物可用作转染试剂。}

4. 发明申请

US20050049387A1 ACID-SENSITIVE POLYACETALS AND METHODS 失效
标题翻译：酸敏感聚合物和方法
公开(公告)号：US20050049387A1
公开(公告)日：2005-03-03
申请号：US10651394
申请日：2003-08-28
申请人： Sang Van , Nitnara Viroonchatapan , Shouping Ji , Lei Yu
发明人： Sang Van , Nitnara Viroonchatapan , Shouping Ji , Lei Yu
IPC分类号： C07C229/22 , C08G4/00 , C08G73/02 , C08G63/02
CPC分类号： C08G4/00 , C07C229/22 , C08G73/028
摘要： Polyacetals comprising a recurring units represented by a formula selected from the group consisting of formula (I) and (II): wherein A comprises at least one acetal group; B is selected from the group consisting of —CH—, —CH(CH3)—, CH2CH—, —CH2C(CH3)—, —CH(CH3)CH—, and —CHCH(CH3)CH(CH3)—; Z is selected from the group consisting of C(O)OR1, C(O)SR1, C(O)NR1R2, and VU; V is a linker group; U is selected from the group consisting of poly(ethyleneimine), poly(propyleneimine), poly(lysine), PAMAM dendrimer, octaamine dendrimer, hexadecaamine dendrimer, enhancer, and targeting receptor; R1 and R2 are each individually selected from the group consisting of hydrogen, C1 to C10 alkyl, and C6 to C10 aryl; D is a linkage selected from the group consisting of carboxylic amide, carboxylic ester, urea, and urethane; and G is selected from the group consisting of C4 to C20 alkyl, C6-C10 aryl, and —(OCH2CH2)n—, where n is in the range of 1 to about 250, are useful in nucleic acid delivery applications. In preferred embodiments, complexes formed between polyacetals of the formulae (I) or (II) and polynucleotides are useful as transfection reagents.
摘要翻译：包含由选自式（I）和（II）的式所表示的重复单元的聚缩醛：其中A包含至少一个缩醛基; B选自-CH-，-CH（CH 3） - ，CH 2 CH - ， - CH 2 C（CH 3） - ， - CH（CH 3）CH-和-CHCH（CH 3）CH（CH 3） Z选自C（O）OR 1，C（O）SR 1，C（O）NR 1 R 2和VU; V是连接基团; U选自聚（亚乙基亚胺），聚（丙烯亚胺），聚（赖氨酸），PAMAM树枝状聚合物，八胺树枝状聚合物，十六胺树枝状聚合物，增强剂和靶向受体; R 1和R 2各自独立地选自氢，C 1至C 10烷基和C 6至C 10芳基; D是选自羧酸酰胺，羧酸酯，脲和氨基甲酸酯的连接体; 并且G选自C 4 -C 20烷基，C 6 -C 10芳基和 - （OCH 2 CH 2）n - ，其中n在1至约250的范围内，可用于核酸递送应用。在优选的实施方案中，在式（I）或（II）的聚缩醛和多核苷酸之间形成的络合物可用作转染试剂。

5. 发明授权

US06878374B2 Biodegradable polyacetals 失效
标题翻译：可生物降解聚缩醛
公开(公告)号：US06878374B2
公开(公告)日：2005-04-12
申请号：US10375705
申请日：2003-02-25
申请人： Lei Yu , Sang Van , Shouping Ji , Kenji Matsumoto
发明人： Lei Yu , Sang Van , Shouping Ji , Kenji Matsumoto
IPC分类号： A61K47/48 , C08G65/00 , C08G73/02 , A61K39/44 , C08F283/00
CPC分类号： C08G73/0213 , A61K47/59 , C08G65/002
摘要： A polymer comprising recurring units represented by formula (I): wherein X is selected from the group consisting of C(O)OR1, C(O)SR1, C(O)NR1R2, and VZ, where R1 and R2 are each individually selected from the group consisting of hydrogen, C1 to C10 alkyl, and C6 to C10 aryl, where V is a labile linker group, and where Z is selected from the group consisting of poly(ethyleneimine), poly(propyleneimine), poly(lysine), PAMAM dendrimer, octaamine dendrimer, and hexadecaamine dendrimer; and wherein Y is selected from the group consisting of —(CH2)2—, —(CH2)2—O—(CH2)2—, —(CH2)2—O—(CH2)2—, and —(CH2)3—NHC(O)—(CH2)6—C(O)NH—(CH2)3— is useful in nucleic acid delivery applications. Polyacetals of the formula (I) are preferably made by reacting appropriate diols and divinyl ethers. In preferred embodiments, complexes formed between polyacetals of the formula (I) and polynucleotides are useful as transfection reagents.
摘要翻译：一种包含由式（I）表示的重复单元的聚合物：其中X选自C（O）OR 1，C（O）SR 1，C（O）NR 1 R 2 >和VZ，其中R 1和R 2各自独立地选自氢，C 1至C 10烷基和C 6至C 10芳基，其中V是不稳定的连接基团，并且其中Z被选择由聚（乙烯亚胺），聚（丙烯亚胺），聚（赖氨酸），PAMAM树枝状聚合物，八胺树枝状聚合物和十六胺树枝状聚合物组成的组中; 其中Y选自 - （CH 2）2 - ， - （CH 2）2 -O-（CH 2）2 - ， - （CH 2）2 -O-（CH 2）2 - 和 - （CH 2） 3-NHC（O） - （CH 2）6 -C（O）NH-（CH 2）3 - 可用于核酸递送应用。式（I）的聚缩醛优选通过使适当的二醇和二乙烯基醚反应来制备。在优选的实施方案中，在式（I）的聚缩醛和多核苷酸之间形成的络合物可用作转染试剂。

6. 发明申请

US20060147376A1 Controllably degradable polymeric biomolecule or drug carrier and method of synthesizing said carrier 审中-公开
公开(公告)号：US20060147376A1
公开(公告)日：2006-07-06
申请号：US11364596
申请日：2006-02-27
申请人： Lei Yu , Fusheng Du , Shouping Ji , Kenji Matsumoto
发明人： Lei Yu , Fusheng Du , Shouping Ji , Kenji Matsumoto
IPC分类号： C40B40/02 , G01N33/53 , A61K49/00
CPC分类号： C08G73/02 , A61K47/59 , A61K47/593 , A61K47/60 , A61K47/645 , A61K48/0041
摘要： The present invention provides a controllably degradable cationic polymer for delivery of biomolecules (nucleic acids, peptides, etc.), drugs, molecules used in medical imaging applications, sensitizing agents used in cancer treatments, and molecules used in tissue engineering. The present invention also provides a method for synthesizing the polymer according to the present invention.

7. 发明申请

US20070072171A1 Solid surface for biomolecule delivery and high-throughput assay 失效
标题翻译：用于生物分子递送和高通量测定的固体表面
公开(公告)号：US20070072171A1
公开(公告)日：2007-03-29
申请号：US11527134
申请日：2006-09-26
申请人： Lei Yu , Kenji Matsumoto , Shouping Ji , Fusheng Du
发明人： Lei Yu , Kenji Matsumoto , Shouping Ji , Fusheng Du
IPC分类号： C12Q1/00 , C12N5/06 , C12N5/04 , C12N15/82 , C12N15/09
CPC分类号： C12N15/87 , A61K47/644 , A61K47/645 , A61K48/00
摘要： The present invention is related to a method for introducing biomolecules, such as nucleic acids, into cells by culturing cells on a solid surface which is coated with a transfection reagents and biomolecules.
摘要翻译：本发明涉及通过在被转染试剂和生物分子包被的固体表面上培养细胞而将生物分子如核酸引入细胞的方法。

8. 发明申请

US20070020761A1 Solid surface for biomolecule delivery and high-throughput assay 审中-公开
公开(公告)号：US20070020761A1
公开(公告)日：2007-01-25
申请号：US11526927
申请日：2006-09-26
申请人： Lei Yu , Kenji Matsumoto , Shouping Ji , Fusheng Du
发明人： Lei Yu , Kenji Matsumoto , Shouping Ji , Fusheng Du
IPC分类号： C12N15/87 , C12N5/06
CPC分类号： C12N15/87 , A61K47/644 , A61K47/645 , A61K48/00
摘要： The present invention is related to a method for introducing biomolecules, such as nucleic acids, into cells by culturing cells on a solid surface which is coated with a transfection reagents and biomolecules.

9. 发明授权

US08192989B2 Solid surface for biomolecule delivery and high-throughput assay 失效
标题翻译：用于生物分子递送和高通量测定的固体表面
公开(公告)号：US08192989B2
公开(公告)日：2012-06-05
申请号：US11527134
申请日：2006-09-26
申请人： Lei Yu , Kenji Matsumoto , Shouping Ji , Fusheng Du
发明人： Lei Yu , Kenji Matsumoto , Shouping Ji , Fusheng Du
IPC分类号： C12N15/00
CPC分类号： C12N15/87 , A61K47/644 , A61K47/645 , A61K48/00
摘要： The present invention is related to a method for introducing biomolecules, such as nucleic acids, into cells by culturing cells on a solid surface which is coated with a transfection reagents and biomolecules.
摘要翻译：本发明涉及通过在被转染试剂和生物分子包被的固体表面上培养细胞而将生物分子如核酸引入细胞的方法。

10. 发明授权

US07125709B2 Culture device and method for eukaryotic cell transfection 失效
标题翻译：用于真核细胞转染的培养装置和方法
公开(公告)号：US07125709B2
公开(公告)日：2006-10-24
申请号：US10775341
申请日：2004-02-10
申请人： Yasunobu Tanaka , Lei Yu , Shouping Ji
发明人： Yasunobu Tanaka , Lei Yu , Shouping Ji
IPC分类号： C12M1/00 , C12M1/16
CPC分类号： C12N5/0068 , C12N2510/00 , C12N2533/18 , C12N2533/54
摘要： A cell culture device for transfecting a eukaryotic cell is disclosed. The cell culture/transfection device may be a multiwell plate or slide which has been coated with a metal salt such as CaCl2. Methods of using the cell culture device to transfect mammalian cells and/or to monitor cell transfection are described. Kits are also described which include the cell transfection device, eukaryotic cells to be transformed and nucleic acid for transformation.
摘要翻译：公开了用于转染真核细胞的细胞培养装置。细胞培养/转染装置可以是已经涂覆有金属盐如CaCl 2 2的多孔板或载玻片。描述了使用细胞培养装置转染哺乳动物细胞和/或监测细胞转染的方法。还描述了包括细胞转染装置，待转化的真核细胞和用于转化的核酸的试剂盒。

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