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1. 发明申请

US20070105150A1 Method for cell surface display of target proteins using fadl of e.coli 有权
标题翻译：使用大肠杆菌的fad1的靶蛋白的细胞表面显示的方法
公开(公告)号：US20070105150A1
公开(公告)日：2007-05-10
申请号：US10588782
申请日：2004-08-30
申请人： Sang Lee , Seung Lee , Si Park
发明人： Sang Lee , Seung Lee , Si Park
IPC分类号： G01N33/53 , C40B40/10 , C40B50/06 , C07H21/04 , C12P21/06 , C12P7/64 , C12N9/18 , C12N9/20
CPC分类号： C12N15/70
摘要： The present invention relates to an expression vector which can effectively express target proteins or peptides on the surface of cells using an outer membrane protein (FadL) of E. coli as a surface anchoring motif. Also, the present invention relates to microorganisms transformed with the expression vector, and a method for stably expressing large amounts of target proteins on the surface of cells by culturing the transformed microorganisms. Furthermore, the present invention relates to a production method of protein arrays, a production method of antibodies, and a bioconversion method, the methods being characterized by using target proteins which have been expressed on the cell surface by the inventive method. In addition, the present invention relates to a method for improving target proteins by the inventive surface expression method. The present invention allows target proteins with normal functions to be expressed on an outer cell membrane. Thus, the present invention will be useful in recombinant live vaccines, the screening of various peptides or antibodies, whole-cell adsorbents for heavy metal removal or waste water treatment, whole-cell bioconversion, and the like.
摘要翻译：本发明涉及使用大肠杆菌的外膜蛋白（FadL）作为表面锚定基序，能够在细胞表面上有效表达靶蛋白或肽的表达载体。此外，本发明涉及用表达载体转化的微生物，以及通过培养转化的微生物在细胞表面上稳定表达大量靶蛋白的方法。此外，本发明涉及蛋白质阵列的制造方法，抗体的制造方法以及生物转化方法，其特征在于，使用通过本发明的方法在细胞表面表达的靶蛋白。此外，本发明涉及通过本发明的表面表达方法改善靶蛋白的方法。本发明允许具有正常功能的靶蛋白在外细胞膜上表达。因此，本发明可用于重组活疫苗，各种肽或抗体的筛选，用于重金属去除或废水处理的全细胞吸附剂，全细胞生物转化等。

2. 发明申请

US20050123994A1 Composition for protecting proteins degradation comprising small heat shock proteins (sHSPs) and method of two-dimensional gel electrophoresis using the sHSPs 失效
标题翻译：用于保护包含小热休克蛋白（sHSP）的蛋白质降解的组合物和使用sHSPs的二维凝胶电泳方法
公开(公告)号：US20050123994A1
公开(公告)日：2005-06-09
申请号：US10791059
申请日：2004-03-02
申请人： Sang Lee , Mee-Jung Han , Si Park
发明人： Sang Lee , Mee-Jung Han , Si Park
IPC分类号： G01N33/483 , C07K14/00 , C07K14/195 , C07K14/245 , C12N15/09 , G01N27/447 , G01N33/68 , G01N33/53
CPC分类号： C07K14/195 , C07K14/00
摘要： The present invention relates to a composition containing sHSPs for prevention of protein degradation and a composition for two-dimensional (2-D) gel electrophoresis. Furthermore, the present invention relates to the improved method of 2-D gel electrophoresis, which is characterized by using sHSPs. According to the present invention, decreasing of protein spots was prevented in the 2-D gel electrophoresis, thereby obtaining 2-D gel with much more protein spots.
摘要翻译：本发明涉及含有用于预防蛋白质降解的sHSP和用于二维（2-D）凝胶电泳的组合物的组合物。此外，本发明涉及2-D凝胶电泳的改进方法，其特征在于使用sHSP。根据本发明，在2-D凝胶电泳中防止了蛋白质斑点的减少，从而获得了具有更多蛋白质斑点的2-D凝胶。

3. 发明申请

US20050037473A1 Process for preparing polyhydroxyalkanoate employing maoC gene 失效
标题翻译：使用maoC基因制备聚羟基链烷酸酯的方法
公开(公告)号：US20050037473A1
公开(公告)日：2005-02-17
申请号：US10662358
申请日：2003-09-16
申请人： Sang Lee , Si Park
发明人： Sang Lee , Si Park
IPC分类号： C12N15/09 , C08G63/06 , C12N1/15 , C12N1/19 , C12N1/21 , C12N9/00 , C12N9/88 , C12N15/11 , C12P7/62 , C07H21/04 , C08G63/78 , C12N9/18
CPC分类号： C12N9/93 , C08G63/06 , C12N9/88 , C12P7/625 , C12Y402/01017
摘要： The present invention relates to a method for producing middle-chain-length polyhydroxyalkanoate (MCL-PHA) using a maoC gene. The producing method of MCL-PHA according to the present invention comprises the steps of transforming a microorganism with the maoC gene to give a transformant, the microorganism being deleted of a fadB gene and containing a PHA synthase gene; culturing the transformant in medium containing a C6-10 carbon source; and obtaining PHA consisting of monomers with 6-10 carbon atoms. When the maoC gene whose function has not yet been established is used according to the present invention, high quality PHA with a higher number of carbon atoms than the prior PHA can be produced at a higher efficiency.
摘要翻译：本发明涉及使用maoC基因生产中链长度聚羟基链烷酸酯（MCL-PHA）的方法。根据本发明的MCL-PHA的制备方法包括以下步骤：用maoC基因转化微生物以产生转化体，所述微生物缺失fadB基因并含有PHA合酶基因; 在含有C6-10碳源的培养基中培养转化体; 并获得由具有6-10个碳原子的单体组成的PHA。当根据本发明使用尚未建立功能的maoC基因时，可以以更高的效率生产具有比先前的PHA高的碳原子数的高质量PHA。

4. 发明申请

US20060194277A1 Method for producing target proteins by deleting or amplifyingibpa and/or ibpb gene coding for inclusion body-associated proteins 失效
标题翻译：通过缺失或扩增编码包涵体相关蛋白的ibpb和/或ibpb基因产生靶蛋白的方法
公开(公告)号：US20060194277A1
公开(公告)日：2006-08-31
申请号：US10545849
申请日：2003-07-10
申请人： Sang Lee , Mee Han , Si Park
发明人： Sang Lee , Mee Han , Si Park
IPC分类号： C12P21/06 , C07H21/04 , C12N9/16 , C07K14/705 , C12N15/74
CPC分类号： C12P21/02 , C07K14/245 , C07K14/5434 , C07K14/57 , C07K14/65 , C12N15/70
摘要： A method for producing target proteins by deleting or amplifying ibpA and/or ibpB genes coding for inclusion body-associated proteins. Two methods for producing target proteins using ibpA and/or ibpB genes coding for inclusion body-associated proteins of E. coli are described, The first method enhances the secretory production and activity of target proteins using ibpA and/or ibpB genes-deleted bacteria. The second method enhances the production of target proteins in the cytoplasm and also converts the target proteins from soluble form to insoluble inclusion body, using ibpA and/or ibpB gene-amplified bacteria.
摘要翻译：通过缺失或扩增编码包涵体相关蛋白的ibpA和/或ibpB基因产生靶蛋白的方法。描述了使用编码大肠杆菌的包涵体相关蛋白的ibpA和/或ibpB基因产生靶蛋白的两种方法。第一种方法使用ibpA和/或ibpB基因缺失的细菌增强靶蛋白的分泌生产和活性。第二种方法增强细胞质中靶蛋白的产生，并使用ibpA和/或ibpB基因扩增的细菌将靶蛋白从可溶形式转化为不溶性包涵体。

5. 发明申请

US20060211105A1 Rhodococcus erythropolis LG12 having acrylic acid degrading activity and method for removing acrylic acid using the same 审中-公开
标题翻译：具有丙烯酸降解活性的红球菌红细菌LG12和使用其的丙烯酸除去方法
公开(公告)号：US20060211105A1
公开(公告)日：2006-09-21
申请号：US11224314
申请日：2005-09-13
申请人： Sanghyun Lee , Junhyeong Cho , Ohjin Park , Joowon Rhee , Si Park
发明人： Sanghyun Lee , Junhyeong Cho , Ohjin Park , Joowon Rhee , Si Park
IPC分类号： C12N1/20 , B09C1/10
CPC分类号： C12R1/01 , C02F3/34
摘要： The present invention relates to a novel strain, Rhodococcus erythropolis LG12, having acrylic acid degrading activity and resistance to acrylic acid, and a method for removing acrylic acid from acrylic acid-containing contaminants using the same. The strain according to the invention has not only acrylic acid degrading activity but also resistance to a high concentration of acrylic acid thus will be useful to remove acrylic acid from acrylic acid-containing contaminants.
摘要翻译：本发明涉及具有丙烯酸降解活性和耐丙烯酸性的新型红细菌红细菌LG12，以及使用该菌株的含丙烯酸污染物除去丙烯酸的方法。根据本发明的应变不仅具有丙烯酸降解活性，而且对高浓度的丙烯酸的耐受性也是有用的，从含丙烯酸的污染物中除去丙烯酸。

6. 发明申请

US20060293433A1 Polymer resin composition and method of preparing the same 有权
标题翻译：聚合物树脂组合物及其制备方法
公开(公告)号：US20060293433A1
公开(公告)日：2006-12-28
申请号：US11432000
申请日：2006-05-11
申请人： Chung Park , Si Park , Worl Kim , Sun Kim , Eon Lee
发明人： Chung Park , Si Park , Worl Kim , Sun Kim , Eon Lee
IPC分类号： C08K3/34
CPC分类号： C08K7/00 , C08K3/013 , C08K2201/016 , Y10T428/2998
摘要： Provided are a polymer resin composition and a method of preparing the same. The polymer resin composition includes: a polymer resin; 0.01-10.0 parts by weight of flaky pigment particles having an aspect ratio of 0.01-0.1 based on 100 parts by weight of the polymer resin; and 0.01-10.0 parts by weight of polyhedral pigment particles having an aspect ratio of 0.300-0.999 based on 100 parts by weight of the polymer resin. Using the polymer resin composition, a polymer resin molded product having excellent appearance, i.e., having a uniform color even in resin flow lines or resin weld lines, and in which there is no degradation in the physical properties of the polymer resin contained therein can be manufactured at a low cost.
摘要翻译：提供一种聚合物树脂组合物及其制备方法。聚合物树脂组合物包括：聚合物树脂; 0.01-10.0重量份基于100重量份聚合物树脂的纵横比为0.01-0.1的片状颜料颗粒; 和0.01-10.0重量份基于100重量份聚合物树脂的纵横比为0.300-0.999的多面体颜料颗粒。使用聚合物树脂组合物，即使在树脂流线或树脂熔接线中也具有优异的外观即均匀的颜色的聚合物树脂模塑制品，其中不含有其中所含的聚合物树脂的物理性能的降低可以是以低成本制造。

7. 发明申请

US20050152667A1 Method for establishing recording quality in digital recording device 审中-公开
标题翻译：在数字录音设备中建立录音质量的方法
公开(公告)号：US20050152667A1
公开(公告)日：2005-07-14
申请号：US11019646
申请日：2004-12-23
申请人： Si Park
发明人： Si Park
IPC分类号： G11B20/10 , G11B19/02 , H04N5/781 , H04N5/85 , H04N9/79 , H04N9/804
CPC分类号： G11B19/025 , H04N5/781 , H04N5/85 , H04N9/7921 , H04N9/8042
摘要： The present invention relates to a method for establishing a recording quality in a digital recording device. In the present invention, still images associated with a plurality of recording qualities having different levels are displayed on a recording-quality selection menu image, and data streams are recorded at a recording quality selected through the recording-quality selection menu image. The still images are generated while a picture captured from video streams being differently encoded at the recording qualities. Otherwise, the still images are pre-stored in the digital recording device while being classified according to the recording qualities, and are read from the digital recording device. Also, the still images are displayed along with information associated with recording times for every recording quality. Therefore, a user easily compares video-data qualities in response to individual recording qualities with one another, and correctly selects a desired recording quality from among a plurality of recording qualities.
摘要翻译：本发明涉及在数字记录装置中建立记录质量的方法。在本发明中，在记录质量选择菜单图像上显示与具有不同等级的多个记录质量相关联的静止图像，并且以通过记录质量选择菜单图像选择的记录质量记录数据流。当从视频流捕获的图像以记录质量不同地被编码时，产生静止图像。否则，根据记录质量将静止图像预先存储在数字记录装置中，并从数字记录装置读取。此外，静止图像与每个记录质量的记录时间相关联的信息一起被显示。因此，用户响应于各个记录质量彼此容易地比较视频数据质量，并且从多个记录质量中正确选择期望的记录质量。

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