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1. 发明授权

US08685893B2 Phage display 有权
标题翻译：噬菌体展示
公开(公告)号：US08685893B2
公开(公告)日：2014-04-01
申请号：US12181222
申请日：2008-07-28
申请人： Sachdev S. Sidhu , Gregory A. Weiss , James A. Wells
发明人： Sachdev S. Sidhu , Gregory A. Weiss , James A. Wells
IPC分类号： C40B40/02
CPC分类号： C40B40/02 , C07K14/005 , C07K2319/00 , C12N15/1037 , C12N2795/10022 , C12N2795/10043
摘要： The transformation yield of electroporation is increased by using higher DNA concentrations and DNA affinity purification. Fusion proteins of a viral coat protein variant and a heterologous polypeptide are useful in phage display systems.
摘要翻译：通过使用更高的DNA浓度和DNA亲和纯化，提高了电穿孔的转化产率。病毒外壳蛋白变体和异源多肽的融合蛋白可用于噬菌体展示系统。

2. 发明申请

US20120165226A1 PHAGE DISPLAY 有权
标题翻译： PHAGE显示
公开(公告)号：US20120165226A1
公开(公告)日：2012-06-28
申请号：US12181222
申请日：2008-07-28
申请人： Sachdev S. SIDHU , Gregory A. Weiss , James A. Wells
发明人： Sachdev S. SIDHU , Gregory A. Weiss , James A. Wells
IPC分类号： C40B40/02 , C12N5/10 , C07K19/00 , C12N15/63 , C40B30/04 , C40B50/00 , C12N7/01 , C12N1/21
CPC分类号： C40B40/02 , C07K14/005 , C07K2319/00 , C12N15/1037 , C12N2795/10022 , C12N2795/10043
摘要： The transformation yield of electroporation is increased by using higher DNA concentrations and DNA affinity purification. Fusion proteins of a viral coat protein variant and a heterologous polypeptide are useful in phage display systems.
摘要翻译：通过使用更高的DNA浓度和DNA亲和纯化，提高了电穿孔的转化产率。病毒外壳蛋白变体和异源多肽的融合蛋白可用于噬菌体展示系统。

3. 发明授权

US08679771B2 Specific N-terminal labeling of peptides and proteins in complex mixtures 有权
标题翻译：复杂混合物中肽和蛋白质的特异性N末端标记
公开(公告)号：US08679771B2
公开(公告)日：2014-03-25
申请号：US12524557
申请日：2008-01-24
申请人： James A. Wells , Sami Mahrus
发明人： James A. Wells , Sami Mahrus
IPC分类号： G01N33/535
CPC分类号： G01N33/6803 , C12Q1/34
摘要： This invention provides general methods for selective labeling of proteins on their N-termini with synthetic peptides. The methods of this invention can be applied to the global proteomic profiling of complex mixtures of proteins and polypeptides.
摘要翻译：本发明提供了用合成肽在其N-末端上选择性标记蛋白质的一般方法。本发明的方法可以应用于蛋白质和多肽的复合物混合物的全局蛋白质组学分析。

4. 发明申请

US20120328628A1 ANTIBODIES TO CONFORMATIONALLY TRAPPED PROTEINS 审中-公开
标题翻译：抗体以一致性捕获蛋白
公开(公告)号：US20120328628A1
公开(公告)日：2012-12-27
申请号：US12307906
申请日：2007-07-06
申请人： James A. Wells , Junjun Gao
发明人： James A. Wells , Junjun Gao
IPC分类号： C07K1/00 , C07H21/04 , C07H21/02 , C07K16/00 , C07K2/00 , A61K38/02 , A61K39/395 , A61K31/7105 , A61K31/711 , C07K1/14 , A61K39/00 , A61P25/16 , A61P35/00 , A61P25/00 , A61P9/10 , A61P29/00 , A61P31/00 , C40B30/04 , G01N33/566 , C07H1/00
CPC分类号： G01N33/68 , C07K16/40 , C07K2299/00 , C07K2317/55 , C07K2317/565 , C07K2317/92 , G01N33/6845 , G01N33/6848 , G01N2500/00
摘要： The present invention provides methods for generating antibodies to specific conformations of proteins. The conformation specific antibodies of the invention can be put to a variety of uses including diagnosis and treatment of diseases and for screening for compounds that induce conformational changes in proteins upon binding.
摘要翻译：本发明提供了产生针对特定蛋白质构象的抗体的方法。本发明的构象特异性抗体可以用于各种用途，包括疾病的诊断和治疗以及用于筛选在结合时诱导蛋白质构象变化的化合物。

5. 发明授权

US07375193B2 Anti-VEGF antibodies 有权
标题翻译：抗VEGF抗体
公开(公告)号：US07375193B2
公开(公告)日：2008-05-20
申请号：US11537015
申请日：2006-09-29
申请人： Manuel Baca , James A. Wells , Leonard G. Presta , Henry B. Lowman , Yvonne Man-Yee Chen
发明人： Manuel Baca , James A. Wells , Leonard G. Presta , Henry B. Lowman , Yvonne Man-Yee Chen
IPC分类号： C07K16/00
CPC分类号： C07K16/22 , C07K2317/24 , C07K2317/55 , C07K2317/56 , C07K2317/565 , C07K2317/567 , C07K2317/73 , C07K2317/76 , C07K2317/92
摘要： Humanized and variant anti-VEGF antibodies and various uses therefor are disclosed. The anti-VEGF antibodies have strong binding affinities for VEGF; inhibit VEGF-induced proliferation of endothelial cells in vitro; and inhibit tumor growth in vivo.
摘要翻译：公开了人源化和变异的抗VEGF抗体及其各种用途。抗VEGF抗体对VEGF具有很强的结合亲和力; 体外抑制VEGF诱导的内皮细胞增殖; 并在体内抑制肿瘤生长。

6. 发明授权

US06197927B1 Peptide variants of protein A 失效
标题翻译：蛋白A的肽变体
公开(公告)号：US06197927B1
公开(公告)日：2001-03-06
申请号：US09036549
申请日：1998-03-06
申请人： Andrew C. Braisted , Melissa A. Starovasnik , James A. Wells
发明人： Andrew C. Braisted , Melissa A. Starovasnik , James A. Wells
IPC分类号： C07K512
CPC分类号： C07K14/31 , C07K2319/00 , Y10S436/828
摘要： Z domain variants of staphylococcal protein A have significantly reduced size but possess IgG-binding affinity equivalent to the wild type Z domain. These Z domain variants are suitable for use in affinity chromatography purification of proteins and in the treatment of staphylococcic diseases.
摘要翻译：葡萄球菌蛋白A的Z结构域变体具有显着减小的大小，但具有与野生型Z结构域相当的IgG结合亲和力。这些Z结构域变体适用于蛋白质的亲和层析纯化和葡萄球菌性疾病的治疗。

7. 发明授权

US6057292A Method for inhibiting growth hormone action 失效
标题翻译：抑制生长激素作用的方法
公开(公告)号：US6057292A
公开(公告)日：2000-05-02
申请号：US28587
申请日：1998-02-24
申请人： Brian C. Cunningham , Henry B. Lowman , James A. Wells , Ross G. Clark , Kenneth Olson , Germaine G. Fuh
发明人： Brian C. Cunningham , Henry B. Lowman , James A. Wells , Ross G. Clark , Kenneth Olson , Germaine G. Fuh
IPC分类号： C12N15/09 , A61K31/00 , A61K38/00 , A61K38/27 , A61K47/48 , A61P3/00 , A61P3/10 , A61P5/00 , A61P5/08 , A61P5/12 , A61P13/12 , A61P35/00 , A61P37/00 , A61P43/00 , C07K1/107 , C07K14/61 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/18 , C12N15/63 , C12P21/02 , C12R1/19
CPC分类号： A61K47/48215 , C07K14/61 , A61K38/00 , C07K2319/00 , Y10S514/866 , Y10S930/12
摘要： Human growth hormone variants are disclosed having enhanced affinity for the growth hormone receptor. Also disclosed are human growth hormone variants conjugated to one or more chemical groups, such as poly(ethylene glycol), which is believed to prolong the in vivo half-life of the variants.
摘要翻译：公开了对生长激素受体具有增强的亲和力的人生长激素变体。还公开了与一种或多种化学基团缀合的人生长激素变体，例如聚（乙二醇），其被认为延长了变体的体内半衰期。

8. 发明授权

US06013478A Method for identifying active domains and amino acid residues in polypeptides and hormone variants 失效
标题翻译：用于鉴定多肽和激素变体中活性结构域和氨基酸残基的方法
公开(公告)号：US06013478A
公开(公告)日：2000-01-11
申请号：US104036
申请日：1998-06-24
申请人： James A. Wells , Brian C. Cunningham
发明人： James A. Wells , Brian C. Cunningham
IPC分类号： A61K38/24 , C07K1/00 , C07K14/575 , C07K14/61 , C07K16/00 , C12N1/21 , C12N15/00 , C12N15/16 , C12N15/18 , C12N15/63 , C12P21/06 , C12Q1/68 , G01N33/48 , G01N33/50 , G01N33/53 , G01N33/566 , G06F19/00
CPC分类号： C07K14/575 , C12N15/1058 , Y10S530/806 , Y10S530/808
摘要： The invention provides methods for the systematic analysis of the structure and function of polypeptides by identifying active domains which influence the activity of the polypeptide with a target substance. Such active domains are determined by substituting selected amino acid segments of the polypeptide with an analogous polypeptide segment from an analog to the polypeptide. The analog has a different activity with the target substance as compared to the parent polypeptide. The activities of the segment-substituted polypeptides are compared to the same activity for the parent polypeptide for the target. A comparison of such activities provides an indication of the location of the active domain in the parent polypeptide. The invention also provides methods for identifying the active amino acid residues within the active domain of the parent polypeptide. The method comprises substituting a scanning amino acid for one of the amino acid residues within the active domain of the parent polypeptide and assaying the residue-substituted polypeptide so formed with a target substance. The invention further provides polypeptide variants comprising segment-substituted and residue-substituted growth hormones, prolactins and placental lactogens.
摘要翻译：本发明提供了通过鉴定影响多肽与靶物质的活性的活性结构域来系统分析多肽的结构和功能的方法。通过用类似物与多肽的类似多肽片段取代多肽的选定氨基酸片段来确定此类活性结构域。与亲本多肽相比，类似物与目标物质具有不同的活性。将段取代的多肽的活性与目标的亲本多肽的相同活性进行比较。这些活性的比较提供了活性结构域在亲本多肽中的位置的指示。本发明还提供鉴定亲本多肽活性结构域内活性氨基酸残基的方法。该方法包括用扫描氨基酸取代母体多肽的活性结构域内的氨基酸残基之一，并测定与靶物质形成的残基取代的多肽。本发明还提供了包含片段取代的和残基取代的生长激素，催乳素和胎盘乳糖的多肽变体。

9. 发明授权

US5854026A Human growth hormone variant having enhanced affinity for human growth hormone receptor at site 1 失效
标题翻译：人类生长激素变体在位点1具有增强的对人生长激素受体的亲和力
公开(公告)号：US5854026A
公开(公告)日：1998-12-29
申请号：US487110
申请日：1995-06-07
申请人： Brian C. Cunningham , James A. Wells
发明人： Brian C. Cunningham , James A. Wells
IPC分类号： A61K38/27 , C07K14/01 , C07K14/475 , C07K14/575 , C07K14/61 , C12N1/21 , C12N15/10 , C12N15/18 , C12N15/62 , C12N15/63 , C40B40/02 , G01N33/68 , G01N33/74
CPC分类号： C40B40/02 , A61K38/27 , C07K14/005 , C07K14/575 , C07K14/61 , C12N15/1037 , C12N15/62 , G01N33/6878 , G01N33/74 , C07K2319/00 , C07K2319/02 , C07K2319/735 , C07K2319/75 , C12N2795/14122 , G01N2333/61 , Y10S930/12
摘要： A human growth hormone variant including the set of amino acid substitutions H18A, Q22A, F25A, D26A, Q29A, E65A, K168A, E174A is disclosed. Also disclosed is a nucleic acid encoding this variant, along with a vector including the nucleic acid, a host cell including the vector, and a process for preparing the variant. The variant has enhanced affinity for human growth hormone receptor at site 1.
摘要翻译：公开了一种人生长激素变体，其包括一组氨基酸取代H18A，Q22A，F25A，D26A，Q29A，E65A，K168A，E174A。还公开了编码该变体的核酸，以及包含核酸的载体，包含载体的宿主细胞，以及制备该变体的方法。该变体在位点1增强了对人生长激素受体的亲合力。

10. 发明授权

US5182204A Non-human carbonyl hydrolase mutants, vectors encoding same and hosts transformed with said vectors 失效
标题翻译：非人羰基水解酶突变体，编码相同的载体和用所述载体转化的宿主
公开(公告)号：US5182204A
公开(公告)日：1993-01-26
申请号：US807786
申请日：1991-12-11
申请人： David A. Estell , Richard R. Bott , Scott D. Power , James A. Wells
发明人： David A. Estell , Richard R. Bott , Scott D. Power , James A. Wells
IPC分类号： C11D3/386 , C12N9/54 , C12N9/56 , C12N15/10 , C12N15/55 , C12N15/75 , C12N1/21
CPC分类号： C12R1/07 , C11D3/386 , C12N15/102 , C12N15/75 , C12N9/54 , Y10S435/832
摘要： Novel carbonyl hydrolase mutants derived from the DNA sequences of naturally-occurring or recombinant non-human carbonyl hydrolases. The mutant carbonyl hydrolases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant carbonyl hydrolase to encode the substitution of an amino acid in the amino acid sequence of a precursor carbonyl hydrolase. Such mutants have properties which are different than the precursor hydrolase.
摘要翻译：衍生自天然或重组非人羰基水解酶的DNA序列的新型羰基水解酶突变体。突变体羰基水解酶通常通过体外修饰编码天然存在或重组的羰基水解酶的前体DNA序列获得，以编码前体羰基水解酶的氨基酸序列中的氨基酸的取代。这些突变体具有与前体水解酶不同的性质。

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