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1. 发明申请

WO2011156539A3 COMPOSITIONS AND METHODS FOR PROTEIN PRODUCTION 审中-公开
公开(公告)号：WO2011156539A3
公开(公告)日：2011-12-15
申请号：PCT/US2011/039701
申请日：2011-06-09
申请人： SYNGENTA PARTICIPATIONS AG , BASU, Shib Sankar , DANG, Kristen Kamerath , AZHAKANANDAM, Kasimalai , MCNEILL, Thomas Z. , GUO, Sheng
发明人： BASU, Shib Sankar , DANG, Kristen Kamerath , AZHAKANANDAM, Kasimalai , MCNEILL, Thomas Z. , GUO, Sheng
IPC分类号： C07H21/02 , C07H21/04
摘要： The present invention provides a method of selecting a mRNA for production of a polypeptide of interest comprising: a) producing an array of individual mRNA sequences comprising different nucleotide sequences encoding the polypeptide of interest; b) determining one or more or two or more of the following parameters for each individual mRNA sequence of (a): (i) minimum free energy (MFE) RNA secondary structure; (ii) ensemble free energy (EFE); (iii) frequency of the minimum free energy (FMFE) RNA secondary structure in a thermodynamic ensemble; and (iv) ensemble diversity (ED); c) ranking the individual mRNA sequences of the array according the parameters determined in step (b); and d) selecting a mRNA sequence from the ranked array of step (c), wherein the selected mRNA produces the polypeptide of interest. The present invention further provides a method of selecting a mRNA for enhanced and reduced production of a polypeptide of interest.

2. 发明授权

EP2580230B1 COMPOSITIONS AND METHODS FOR PROTEIN PRODUCTION 有权
标题翻译：蛋白质生产的组合物和方法
公开(公告)号：EP2580230B1
公开(公告)日：2017-08-23
申请号：EP11793130.3
申请日：2011-06-09
申请人： Syngenta Participations AG
发明人： BASU, Shib Sankar , DANG, Kristen Kamerath , AZHAKANANDAM, Kasimalai , MCNEILL, Thomas Z. , GUO, Sheng
IPC分类号： C07H21/02 , C07H21/04
CPC分类号： C12N15/1089 , C12N15/1034
摘要： The present invention provides a method of selecting a mRNA for production of a polypeptide of interest comprising: a) producing an array of individual mRNA sequences comprising different nucleotide sequences encoding the polypeptide of interest; b) determining one or more or two or more of the following parameters for each individual mRNA sequence of (a): (i) minimum free energy (MFE) RNA secondary structure; (ii) ensemble free energy (EFE); (iii) frequency of the minimum free energy (FMFE) RNA secondary structure in a thermodynamic ensemble; and (iv) ensemble diversity (ED); c) ranking the individual mRNA sequences of the array according the parameters determined in step (b); and d) selecting a mRNA sequence from the ranked array of step (c), wherein the selected mRNA produces the polypeptide of interest. The present invention further provides a method of selecting a mRNA for enhanced and reduced production of a polypeptide of interest.
摘要翻译：本发明提供了选择用于产生感兴趣多肽的mRNA的方法，包括：a）产生包含编码感兴趣多肽的不同核苷酸序列的单个mRNA序列阵列; b）对于（a）的每个单独的mRNA序列确定一个或多个或两个或更多个以下参数：（i）最小自由能（MFE）RNA二级结构; （ii）集合自由能（EFE）; （iii）热力学系综中最小自由能（FMFE）RNA二级结构的频率; 和（四）集合多样性（ED）; c）根据步骤（b）中确定的参数对阵列的单个mRNA序列进行排序; 和d）从步骤（c）的分级阵列中选择mRNA序列，其中所选mRNA产生目的多肽。本发明进一步提供了选择用于增强和减少感兴趣多肽生产的mRNA的方法。

3. 发明公开

EP2580230A2 COMPOSITIONS AND METHODS FOR PROTEIN PRODUCTION 有权
标题翻译：组合物和蛋白质制备方法
公开(公告)号：EP2580230A2
公开(公告)日：2013-04-17
申请号：EP11793130.3
申请日：2011-06-09
申请人： Syngenta Participations AG
发明人： BASU, Shib Sankar , DANG, Kristen Kamerath , AZHAKANANDAM, Kasimalai , MCNEILL, Thomas Z. , GUO, Sheng
IPC分类号： C07H21/02 , C07H21/04
CPC分类号： C12N15/1089 , C12N15/1034
摘要： The present invention provides a method of selecting a mRNA for production of a polypeptide of interest comprising: a) producing an array of individual mRNA sequences comprising different nucleotide sequences encoding the polypeptide of interest; b) determining one or more or two or more of the following parameters for each individual mRNA sequence of (a): (i) minimum free energy (MFE) RNA secondary structure; (ii) ensemble free energy (EFE); (iii) frequency of the minimum free energy (FMFE) RNA secondary structure in a thermodynamic ensemble; and (iv) ensemble diversity (ED); c) ranking the individual mRNA sequences of the array according the parameters determined in step (b); and d) selecting a mRNA sequence from the ranked array of step (c), wherein the selected mRNA produces the polypeptide of interest. The present invention further provides a method of selecting a mRNA for enhanced and reduced production of a polypeptide of interest.

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