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    • 8. 发明申请
    • LIGHT-INDUCED ELECTRON CAPTURE AT A SURFACE
    • 表面光诱导电子捕获
    • WO0077812A8
    • 2002-06-20
    • PCT/US0040173
    • 2000-06-08
    • UNIV NORTHEASTERNWANG POGUANGZHANG XINGIESE ROGER W
    • WANG POGUANGZHANG XINGIESE ROGER W
    • H01J49/16B05D3/06B01D59/34H01J49/04
    • H01J49/0463
    • A method for increasing the performance and usefulness of laser induced electron capture mass spectrometry (LI-EC-MS). Light (photons) from a light source (laser), is used to activate an electron of a surface, preferably a metal surface, where the light energy is below the work function of the surface. The electron is transferred to an analyte on the surface, forming an anionic product from the analyte. The anionic product can simultaneously undergo desorption for detection in a mass spectrometer. Alternatively, the analyte (first substance) can receive an electron from an intermediate compound having a low ionization potential which is deposited with the analyte. This gives a sharper or more intense sig nal from an analyte than prior forms of LI-EC-MS, but utilizes ordinary MS equipment. Further, the procedure even enables detection of species, such as nucleic acids labeled with polyfluoro-containing groups, that previously were beyond the reach of LI-EC-MS techniques.
    • 一种提高激光诱导电子捕获质谱(LI-EC-MS)性能和有用性的方法。 来自光源(激光)的光(光子)用于激活表面的电子,优选金属表面,其中光能低于表面的功函数。 将电子转移到表面上的分析物上,从分析物形成阴离子产物。 阴离子产物可以同时进行解吸,以在质谱仪中进行检测。 或者,分析物(第一物质)可以从沉积有分析物的电离电位低的中间体化合物接收电子。 这使得从分析物中获得比先前形式的LI-EC-MS更尖锐或更强烈的信号,但是使用普通的MS设备。 此外,该方法甚至能够检测以前是LI-EC-MS技术无法达到的物种,例如用含多氟基团标记的核酸。