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1. 发明专利

AU2006242236A1 System and method for a pulsed light source used in fluorescence detection 未知
公开(公告)号：AU2006242236A1
公开(公告)日：2006-11-09
申请号：AU2006242236
申请日：2006-05-02
申请人： STRATAGENE CALIFORNIA
发明人： TAYLOR ROGER H , CHOI HOWARD Y , REID TAYLOR A
IPC分类号： G01J3/30 , G01N21/64
摘要： A system and method for a pulsed light source used in detecting fluorescence from a plurality of samples of biological material discretely, continuously or intermittently during thermal cycling of DNA to accomplish a polymerase chain reaction (PCR). An apparatus for sampling at least one sample of a biological material comprises a light source that emits a pulsed excitation light that interacts with the sample and a detector sensitive to fluorescence emitted from the sample. A method of sampling at least one sample to detect fluorescence comprises generating a pulsed excitation light with a pulsed light source; directing the pulsed excitation light into the sample; illuminating a sample with the pulsed excitation light to generate an emission light; and detecting the optical characteristics of the emission light.

2. 发明专利

CA2607045A1 SYSTEM AND METHOD FOR A PULSED LIGHT SOURCE USED IN FLUORESCENCE DETECTION 未知
公开(公告)号：CA2607045A1
公开(公告)日：2006-11-09
申请号：CA2607045
申请日：2006-05-02
申请人： STRATAGENE CALIFORNIA
发明人： CHOI HOWARD Y , TAYLOR ROGER H , REID TAYLOR A
IPC分类号： G01J3/30 , G01N21/64
摘要： A system and method for a pulsed light source used in detecting fluorescence from a plurality of samples of biological material discretely, continuously or intermittently during thermal cycling of DNA to accomplish a polymerase chain reaction (PCR). An apparatus for sampling at least one sample of a biological material comprises a light source that emits a pulsed excitation light that interacts with the sample and a detector sensitive to fluorescence emitted from the sample. A method of sampling at least one sample to detect fluorescence comprises generating a pulsed excitation light with a pulsed light source; directing the pulsed excitation light into the sample; illuminating a sample with the pulsed excitation light to generate an emission light; and detecting the optical characteristics of the emission light.

3. 发明专利

AU2002327800B2 Adaptive baseline algorithm for quantitative PCR 未知
公开(公告)号：AU2002327800B2
公开(公告)日：2007-12-20
申请号：AU2002327800
申请日：2002-10-02
申请人： STRATAGENE CALIFORNIA
发明人： TAYLOR ROGER H , ORR ALAN R
IPC分类号： G01N21/78 , C12N15/00 , C12P19/34 , C12Q1/68 , G01N33/48 , G01N33/50 , G06F19/00
摘要： The invention relates to baseline subtraction algorithms developed to reduce tube-to-tube and cycle-to-cycle variabilities during real time PCR amplification. Particularly, the invention relates to algorithms for determining the threshold cycle for the first reliable detection of the amplified nucleic acid product. The invention also relates to computer programs comprising the algorithm and methods and/systems implementing the algorithm.

4. 发明专利

CA2610856A1 SYSTEM AND METHOD FOR FLUORESCENCE EXCITATION AND DETECTION HAVING DISTINCT OPTICAL PATHS 未知
公开(公告)号：CA2610856A1
公开(公告)日：2006-12-28
申请号：CA2610856
申请日：2006-06-12
申请人： STRATAGENE CALIFORNIA
发明人： ZAJAC KENNETH J , REID TAYLOR A , TAYLOR ROGER H
IPC分类号： G01N21/01
摘要： A system and method for fluorescence excitation and detection having distinc t optical paths is disclosed. A system for detecting fluorescence comprises a light source(40) that emits an excitation light (48) into an illumination tu be (44); a plurality of collection optics (39) located around an aperture (47) in the illumination tube for collecting fluorescence; and a detector (53) for determining the amount of fluorescence. A method for detecting fluorescence comprises emitting an excitation light (48) from a light source (40) into an illumination tube (44); directing the excitation light to an excitation filt er (62); illuminating a sample with the excitation light (48) to generate an emission light; and detecting the optical characteristics of the emission light using a plurality of collection optics (39) located around the illumination tube (44).

5. 发明专利

AU2006259569A1 System and method for fluorescence excitation and detection having distinct optical paths 未知
公开(公告)号：AU2006259569A1
公开(公告)日：2006-12-28
申请号：AU2006259569
申请日：2006-06-12
申请人： STRATAGENE CALIFORNIA
发明人： REID TAYLOR A , ZAJAC KENNETH J , TAYLOR ROGER H
IPC分类号： G01N21/01
摘要： A system and method for fluorescence excitation and detection having distinct optical paths is disclosed. A system for detecting fluorescence comprises a light source that emits an excitation light into an illumination tube; a plurality of collection optics located around an aperture in the illumination tube for collecting fluorescence; and a detector for determining the amount of fluorescence. A method for detecting fluorescence comprises emitting an excitation light from a light source into an illumination tube; directing the excitation light to an excitation filter; illuminating a sample with the excitation light to generate an emission light; and detecting the optical characteristics of the emission light using a plurality of collection optics located around the illumination tube.

6. 发明公开

EP1893975A4 SYSTEM AND METHOD FOR FLUORESCENCE EXCITATION AND DETECTION HAVING DISTINCT OPTICAL PATHS 审中-公开
标题翻译：系统和方法用于荧光激发和检测具有不同的光路
公开(公告)号：EP1893975A4
公开(公告)日：2010-08-04
申请号：EP06784804
申请日：2006-06-12
申请人： STRATAGENE CALIFORNIA
发明人： REID TAYLOR A , TAYLOR ROGER H , ZAJAC KENNETH J
IPC分类号： G01N21/01
CPC分类号： G01J3/10 , G01J3/4406 , G01J2003/1213 , G01N21/645 , G01N21/6452 , G01N21/6486 , G01N2021/6484 , G01N2201/0446 , G01N2201/0612 , G01N2201/062

7. 发明公开

EP1438430A4 ADAPTIVE BASELINE ALGORITHM FOR QUANTITATIVE PCR 审中-公开
标题翻译：算法基线平衡定量PCR
公开(公告)号：EP1438430A4
公开(公告)日：2005-05-25
申请号：EP02763814
申请日：2002-10-02
申请人： STRATAGENE CALIFORNIA
发明人： TAYLOR ROGER H , ORR ALAN R
IPC分类号： G01N21/78 , C12N15/00 , C12P19/34 , C12Q1/68 , G01N33/48 , G01N33/50 , G06F19/00 , C07H19/00 , C07H21/02 , C07H21/04
CPC分类号： G01N21/6428 , C12Q1/6851 , C12Q2537/165
摘要： The invention relates to baseline subtraction algorithms developed to reduce tube-to-tube and cycle-to-cycle variabilities during real time PCR amplification. Particularly, the invention relates to algorithms for determining the threshold cycle for the first reliable detection of the amplified nucleic acid product. The invention also relates to computer programs comprising the algorithms and methods and/systems implementing the algorithms.

8. 发明申请

WO2006138261A3 SYSTEM AND METHOD FOR FLUORESCENCE EXCITATION AND DETECTION HAVING DISTINCT OPTICAL PATHS 审中-公开
标题翻译：用于荧光激发和检测的系统和方法具有不同的光学PATHS
公开(公告)号：WO2006138261A3
公开(公告)日：2007-09-07
申请号：PCT/US2006022908
申请日：2006-06-12
申请人： STRATAGENE CALIFORNIA , REID TAYLOR A , TAYLOR ROGER H , ZAJAC KENNETH J
发明人： REID TAYLOR A , TAYLOR ROGER H , ZAJAC KENNETH J
IPC分类号： G01N21/01
CPC分类号： G01J3/10 , G01J3/4406 , G01J2003/1213 , G01N21/645 , G01N21/6452 , G01N21/6486 , G01N2021/6484 , G01N2201/0446 , G01N2201/0612 , G01N2201/062
摘要： A system and method for fluorescence excitation and detection having distinct optical paths is disclosed. A system for detecting fluorescence comprises a light source(40) that emits an excitation light (48) into an illumination tube (44); a plurality of collection optics (39) located around an aperture (47) in the illumination tube for collecting fluorescence; and a detector (53) for determining the amount of fluorescence. A method for detecting fluorescence comprises emitting an excitation light (48) from a light source (40) into an illumination tube (44); directing the excitation light to an excitation filter (62); illuminating a sample with the excitation light (48) to generate an emission light; and detecting the optical characteristics of the emission light using a plurality of collection optics (39) located around the illumination tube (44).
摘要翻译：公开了具有不同光路的用于荧光激发和检测的系统和方法。用于检测荧光的系统包括将激发光（48）发射到照明管（44）中的光源（40）; 位于所述照明管中用于收集荧光的孔（47）周围的多个收集光学器件（39）和用于确定荧光量的检测器（53）。检测荧光的方法包括将光源（40）的激发光（48）发射到照明管（44）中; 将激发光引导到激发过滤器（62）; 用激发光（48）照射样品以产生发射光; 以及使用位于所述照明管（44）周围的多个收集光学器件（39）来检测所述发射光的光学特性。

9. 发明申请

WO2006119277A3 SYSTEM AND METHOD FOR A PULSED LIGHT SOURCE USED IN FLUORESCENCE DETECTION 审中-公开
标题翻译：用于荧光检测中使用的脉冲光源的系统和方法
公开(公告)号：WO2006119277A3
公开(公告)日：2007-03-15
申请号：PCT/US2006016808
申请日：2006-05-02
申请人： STRATAGENE CALIFORNIA , TAYLOR ROGER H , REID TAYLOR A , CHOI HOWARD Y
发明人： TAYLOR ROGER H , REID TAYLOR A , CHOI HOWARD Y
IPC分类号： G01J3/30 , G01N21/64
CPC分类号： G01J3/10 , G01J3/4406 , G01J2001/4242 , G01N21/645 , G01N21/6452 , G01N21/6486
摘要： A system and method for a pulsed light source (40) used in detecting fluorescence from a plurality of samples (94) of biological material discretely, continuously or intermittently during thermal cycling of DNA to accomplish a polymerase chain reaction (PCR). An apparatus for sampling at least one sample (94) of a biological material comprises a light source (40) that emits a pulsed excitation light (42) that interacts with the sample (94) and a detector (50) sensitive to fluorescence emitted from the sample (94). A method of sampling at least one sample (94) to detect fluorescence comprises generating a pulsed excitation light (42) with a pulsed light source (40); directing the pulsed excitation light (42) into the sample (94); illuminating a sample (94) with the pulsed excitation light (42) to generate an emission light; and detecting the optical characteristics of the emission light.
摘要翻译：一种脉冲光源（40）的系统和方法，用于在DNA的热循环期间离散地，连续地或间歇地检测来自生物材料的多个样品（94）的荧光，以完成聚合酶链式反应（PCR）。用于对生物材料的至少一个样品（94）进行采样的装置包括发射与样品（94）相互作用的脉冲激发光（42）的光源（40）和与样品（94）相互作用的检测器（50）样品（94）。对至少一个样品（94）取样以检测荧光的方法包括用脉冲光源（40）产生脉冲激发光（42）; 将脉冲激发光（42）引导到样品（94）中; 用脉冲激发光（42）照射样品（94）以产生发射光; 并检测发射光的光学特性。

10. 发明申请

WO2007027634A3 COMPACT OPTICAL MODULE FOR FLUORESCENCE EXCITATION AND DETECTION 审中-公开
标题翻译：用于荧光激发和检测的紧凑光学模块
公开(公告)号：WO2007027634A3
公开(公告)日：2007-12-06
申请号：PCT/US2006033576
申请日：2006-08-25
申请人： STRATAGENE CALIFORNIA , REID TAYLOR A , TAYLOR ROGER H
发明人： REID TAYLOR A , TAYLOR ROGER H
IPC分类号： G21H3/02
CPC分类号： G01N21/6452 , G01N21/6428 , G01N2201/062 , G01N2201/0642 , G01N2201/101 , H01L31/167 , H01L2224/48472 , H01L2924/10253 , H01L2924/3025 , H01L2224/48091 , H01L2924/00014 , H01L2924/00
摘要： A compact optical module for fluorescence excitation and detection and methods for using same are disclosed. An apparatus for detecting fluorescence includes a substrate base, a detector adjacent to the substrate base for determining the amount of fluorescence, an emission filter adjacent to the detector, a light source for emitting an excitation light, the light source engaging the emission filter, and a cover formed over the detector, the emission filter, and the light source.
摘要翻译：公开了用于荧光激发和检测的紧凑型光学模块及其使用方法。用于检测荧光的装置包括：基板，与基板基板相邻的用于确定荧光量的检测器，与检测器相邻的发射滤光器，用于发射激发光的光源，与发射滤光器接合的光源;以及形成在检测器，发射滤光器和光源上的盖。

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