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1. 发明申请

WO2002061126A2 THE PREPARATION OF POLYNUCLEOTIDE ARRAYS 审中-公开
标题翻译：多核苷酸阵列的制备
公开(公告)号：WO2002061126A2
公开(公告)日：2002-08-08
申请号：PCT/GB2002/000438
申请日：2002-01-30
申请人： SOLEXA LTD. , BARNES, Colin , BALASUBRAMANIAN, Shankar , KLENERMAN, David , OSBORNE, Mark, Allen
发明人： BARNES, Colin , BALASUBRAMANIAN, Shankar , KLENERMAN, David , OSBORNE, Mark, Allen
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , B01J19/0046 , B01J2219/00497 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00626 , B01J2219/00659 , B01J2219/00707 , B01J2219/00722 , C12Q2525/301 , C40B40/06 , C12Q2565/507 , C12Q2565/107 , C12Q2525/197 , C12Q2565/619
摘要： A device comprising an array of molecules immobilised on a solid surface is disclosed, wherein the array has a high density of relatively short molecules and relatively long polynucleotides immobilised on the surface of a solid support, wherein the polynucleotides are at a density that permits individual resolution of those parts thereof that extend beyond the relatively short molecules.
摘要翻译：公开了一种包含固定在固体表面上的分子阵列的装置，其中所述阵列具有固定在固体支持物表面上的相对较短分子的高密度和相对长的多核苷酸，其中所述多核苷酸处于允许单独分辨的密度的那些部分延伸超过相对短的分子。

2. 发明申请

WO2003016565A3 DNA SEQUENCE ANALYSIS 审中-公开
公开(公告)号：WO2003016565A3
公开(公告)日：2003-02-27
申请号：PCT/GB2002/003750
申请日：2002-08-13
申请人： SOLEXA LTD , BALASUBRAMANIAN, Shankar , KLENERMAN, David , BARNES, Colin , WILLIAMSON, Alan, Rowe
发明人： BALASUBRAMANIAN, Shankar , KLENERMAN, David , BARNES, Colin , WILLIAMSON, Alan, Rowe
IPC分类号： C12Q1/68
摘要： The present invention concerns a method for determining the identity of one or more single nucleotide polymorphisms (SNP) in a genome, comprising: (i) fragmenting a sample genome; (ii) contacting the fragments with an excess of a plurality of different oligonucleotide primers under conditions that permit a primer to form a duplex with a complementary region on a fragment, each primer having a predetermined sequence complementary to a sequence on the genome that is proximal to a putative SNP site, and the resulting duplexes being immobilised on a solid support; (iii) carrying out the sequencing reaction(s) and detecting the incorporation of bases onto the oligonucleotide primers to extend the primers to at least the SNP site; and(iv) comparing the resulting sequences to those of the reference one or more SNPs.

3. 发明申请

WO2002061127A2 ARRAYED POLYNUCLEOTIDES AND THEIR USE IN GENOME ANALYSIS 审中-公开
标题翻译：阵列多核苷酸及其在基因组分析中的应用
公开(公告)号：WO2002061127A2
公开(公告)日：2002-08-08
申请号：PCT/GB2002/000439
申请日：2002-01-30
申请人： SOLEXA LTD. , BARNES, Colin , BALASUBRAMANIAN, Shankar , KLENERMAN, David
发明人： BARNES, Colin , BALASUBRAMANIAN, Shankar , KLENERMAN, David
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , B01J19/0046 , B01J2219/00497 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00626 , B01J2219/00659 , B01J2219/00707 , B01J2219/00722 , C12Q2525/301 , C40B40/06 , C12Q2565/507 , C12Q2565/107 , C12Q2525/197 , C12Q2565/619
摘要： A method for determining a single nucleotide polymorphism present in a genome, comprises the steps of: (i) immobilising fragments of said genome onto the surface of a solid support to form an array of polynucleotide molecules capable of interrogation, wherein the array allows the molecules to be individually resolved by optical microscopy, and wherein each molecule is immobilised by covalent bonding to the surface, other than at part of each molecule that can be interrogated; (ii) identifying nucleotides at selected positions in the genome; and (iii) comparing the results of step (ii) with a known consensus sequence, and identifying any differences between the consensus sequence and said genome.
摘要翻译：确定存在于基因组中的单核苷酸多态性的方法包括以下步骤：（i）将所述基因组的片段固定在固体支持物的表面上以形成能够询问的多核苷酸分子阵列，其中该阵列允许分子通过光学显微镜单独解析，并且其中每个分子通过共价键合固定在表面上，而不是每个可被询问的分子的部分; （ii）鉴定基因组中选定位置的核苷酸; 和（iii）将步骤（ii）的结果与已知的共有序列比较，并鉴定共有序列和所述基因组之间的任何差异。

4. 发明申请

WO2005119265A1 PROTEIN DETECTION 审中-公开
标题翻译：蛋白质检测
公开(公告)号：WO2005119265A1
公开(公告)日：2005-12-15
申请号：PCT/GB2005/002153
申请日：2005-06-01
申请人： CAMBRIDGE UNIVERSITY TECHNICAL SERVICES LIMITED , KLENERMAN, David , LI, Haitao
发明人： KLENERMAN, David , LI, Haitao
IPC分类号： G01N33/68
CPC分类号： G01N33/6803 , G01N33/54306 , G01N33/582
摘要： Provided are methods and kits for detecting a protein analyte in a sample, the methods comprising the steps of: (i) providing a sample solution, (ii) providing at least two ligands (e.g. antiboides) capable of binding specifically to the protein analyte, each ligand being labelled with a different fluorophore, which fluorophores are excitable by spectrally separated wavelengths of light, (iii) combining the sample solution and the ligands to form a detection sample such that protein analyte if present is labelled with the at least two ligands, and wherein each ligand is present at a concentration of less than 1 nM,(iv) illuminating the sample solution with at least two coherent light beams having wavelengths capable of exciting the fluorophores, which beams coincide to form a coincident probe volume within the sample solution, (v) detecting the number of coincident fluorescent events within the coincident probe volume over a period of time. The sensitive detection system can be used to detect analytes at very low concentrations, for example in body fluids.
摘要翻译：提供了用于检测样品中的蛋白质分析物的方法和试剂盒，所述方法包括以下步骤：（i）提供样品溶液，（ii）提供能够特异性结合蛋白质分析物的至少两种配体（例如抗体）每个配体用不同的荧光团标记，该荧光团可通过光谱分离的光波长激发，（iii）将样品溶液和配体结合以形成检测样品，使得如果存在的蛋白质分析物用至少两种配体标记，并且其中每个配体以小于1nM的浓度存在，（iv）用具有能够激发荧光团的波长的至少两个相干光束照射样品溶液，该光束重合以在样品溶液内形成一致的探针体积，（v）在一段时间内检测符合探针体积内的重合荧光事件的数量。敏感检测系统可用于以非常低的浓度检测分析物，例如体液。

5. 发明申请

WO2002102504A2 THE PRODUCTION OF MOLECULAR ARRAYS 审中-公开
标题翻译：分子阵列的生产
公开(公告)号：WO2002102504A2
公开(公告)日：2002-12-27
申请号：PCT/GB2002/002727
申请日：2002-06-13
申请人： IMPERIAL COLLEGE INNOVATIONS LTD. , KORCHEV, Yuri, Evgenievich , KLENERMAN, David , ABELL, Christopher , YING, Liming , BRUCKBAUER, Andreas
发明人： KORCHEV, Yuri, Evgenievich , KLENERMAN, David , ABELL, Christopher , YING, Liming , BRUCKBAUER, Andreas
IPC分类号： B01J19/00
CPC分类号： B01J19/0046 , B01J2219/00364 , B01J2219/00371 , B01J2219/00533 , B01J2219/00576 , B01J2219/00608 , B01J2219/00612 , B01J2219/00637 , B01J2219/00722 , B01J2219/00725 , G01Q80/00 , Y10S977/924
摘要： A method for the production of an array of molecules immobilised on a substrate, comprises the deposition of the molecules from a micropipette containing them, onto the substrate, in a liquid environment, wherein the distance of the micropipette from the substrate is controlled in response to the ion current in the liquid. This method is particularly suitable for the deposition of biological molecules.
摘要翻译：用于制备固定在基底上的分子阵列的方法包括在液体环境中将分子从含有它们的微量移液管沉积到基底上，其中微量移液管与基底的距离响应于液体中的离子电流。该方法特别适用于沉积生物分子。

6. 发明申请

WO2009095720A2 SCANNING PROBE MICROSCOPY 审中-公开
标题翻译：扫描探针显微镜
公开(公告)号：WO2009095720A2
公开(公告)日：2009-08-06
申请号：PCT/GB2009/050092
申请日：2009-02-02
申请人： Imperial Innovations Limited , NOVAK, Pavel , LI, Chao , SHEVCHUK, Andrew , OSTANIN, Victor , KLENERMAN, David , KORCHEV, Yuri , FROLENKOV, Gregory , CLARKE, Richard
发明人： NOVAK, Pavel , LI, Chao , SHEVCHUK, Andrew , OSTANIN, Victor , KLENERMAN, David , KORCHEV, Yuri , FROLENKOV, Gregory , CLARKE, Richard
IPC分类号： G01N13/12 , G12B21/02
CPC分类号： G01Q60/44 , B82Y35/00 , G01Q10/00 , G01Q10/02 , G01Q10/065
摘要： A method for interrogating a surface using scanning ion conductance microscopy (SICM), comprising the steps of: a) repeatedly bringing a SICM probe into proximity with the surface at discrete, spaced locations in a region of the surface and measuring surface height at each location; b) estimating surface roughness or other characteristic for the region based upon the surface height measurements; and c) repeatedly bringing the probe into proximity with the surface at discrete, spaced locations in the region, the number and location of which is based upon the estimated surface roughness or other characteristic in the region, and obtaining an image of the region with a resolution adapted to the surface roughness or other characteristic.
摘要翻译：一种使用扫描离子电导显微镜（SICM）询问表面的方法，包括以下步骤：a）在表面的一个区域中的离散的间隔位置处重复地使SICM探针接近表面，并在每个位置测量表面高度 ; b）基于表面高度测量来估计区域的表面粗糙度或其他特性; 以及c）在所述区域中的离散的间隔位置处重复地使所述探针靠近所述表面，所述位置的数量和位置基于所估计的表面粗糙度或所述区域中的其它特征，并且获得所述区域的图像分辨率适应于表面粗糙度或其他特性。

7. 发明授权

EP1105529B1 ARRAYED BIOMOLECULES AND THEIR USE IN SEQUENCING 有权
标题翻译： DIES生物分子与它们在测序
公开(公告)号：EP1105529B1
公开(公告)日：2005-11-09
申请号：EP99936809.5
申请日：1999-07-30
申请人： Solexa Ltd.
发明人： BALASUBRAMANIAN, Shankar , KLENERMAN, David , BARNES, Colin , OSBORNE, Mark Allen
IPC分类号： C12Q1/68 , B01J19/00
CPC分类号： C40B30/04 , B01J19/0046 , B01J2219/00317 , B01J2219/00497 , B01J2219/00527 , B01J2219/00529 , B01J2219/0054 , B01J2219/00572 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00648 , B01J2219/00659 , B01J2219/00677 , B01J2219/00702 , B01J2219/00707 , B01J2219/00722 , C12Q1/6837 , C12Q2525/301 , C40B40/06 , C40B60/14 , C12Q2565/507
摘要： According to the present invention, a device comprising an array of molecules immobilised on a solid surface is disclosed, wherein the array has a surface density which allows each molecule to be individually resolved, e.g. by optical microscopy. Therefore, the arrays of the present invention consist of single molecules that are more spatially distinct than the arrays of the prior art.

8. 发明公开

EP1105529A1 ARRAYED BIOMOLECULES AND THEIR USE IN SEQUENCING 有权
标题翻译： DIES生物分子与它们在测序
公开(公告)号：EP1105529A1
公开(公告)日：2001-06-13
申请号：EP99936809.5
申请日：1999-07-30
申请人： Solexa Ltd.
发明人： BALASUBRAMANIAN, Shankar , KLENERMAN, David
IPC分类号： C12Q1/68 , B01J19/00
CPC分类号： C40B30/04 , B01J19/0046 , B01J2219/00317 , B01J2219/00497 , B01J2219/00527 , B01J2219/00529 , B01J2219/0054 , B01J2219/00572 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00648 , B01J2219/00659 , B01J2219/00677 , B01J2219/00702 , B01J2219/00707 , B01J2219/00722 , C12Q1/6837 , C12Q2525/301 , C40B40/06 , C40B60/14 , C12Q2565/507
摘要： According to the present invention, a device comprising an array of molecules immobilised on a solid surface is disclosed, wherein the array has a surface density which allows each molecule to be individually resolved, e.g. by optical microscopy. Therefore, the arrays of the present invention consist of single molecules that are more spatially distinct than the arrays of the prior art.

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