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1. 发明申请

WO03048387A3 LABELLED NUCLEOTIDES 审中-公开
标题翻译：标签核心
公开(公告)号：WO03048387A3
公开(公告)日：2003-10-16
申请号：PCT/GB0205474
申请日：2002-12-04
申请人： SOLEXA LTD , BALASUBRAMANIAN SHANKAR , BARNES COLIN , LIU XIAOHAI , SWERDLOW HAROLD
发明人： BALASUBRAMANIAN SHANKAR , BARNES COLIN , LIU XIAOHAI , SWERDLOW HAROLD
IPC分类号： G01N33/53 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C07H21/04 , C12N15/09 , G01N33/533 , G01N33/566 , C12Q1/68
CPC分类号： C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C12Q1/6869 , C12Q2525/186 , C12Q2535/113 , C12Q2525/197
摘要： Nucleosides and nucleotides are disclosed that are linked to detectable labels via a cleavable linker group.
摘要翻译：公开了通过可切割连接基团与可检测标记物连接的核苷和核苷酸。

2. 发明申请

WO2007010251A3 PREPARATION OF TEMPLATES FOR NUCLEIC ACID SEQUENCING 审中-公开
标题翻译：核酸序列测定模板的制备
公开(公告)号：WO2007010251A3
公开(公告)日：2007-08-30
申请号：PCT/GB2006002687
申请日：2006-07-20
申请人： SOLEXA LTD , LIU XIAOHAI , MILTON JOHN , SMITH GEOFFREY PAUL , BARNES COLIN , RASOLONJATOVO ISABELLE MARIE J , RIGATTI ROBERTO , WU XIAOLIN , OST TOBIAS WILLIAM BARR , WORSLEY GRAHAM JOHN , EARNSHAW DAVID JAMES , TURCATTI GERARDO , ROMIEU ANTHONY
发明人： LIU XIAOHAI , MILTON JOHN , SMITH GEOFFREY PAUL , BARNES COLIN , RASOLONJATOVO ISABELLE MARIE J , RIGATTI ROBERTO , WU XIAOLIN , OST TOBIAS WILLIAM BARR , WORSLEY GRAHAM JOHN , EARNSHAW DAVID JAMES , TURCATTI GERARDO , ROMIEU ANTHONY
IPC分类号： C12Q1/68
CPC分类号： B01J19/0046 , B01J2219/00596 , B01J2219/00608 , B01J2219/00675 , B01J2219/00722 , C12Q1/6806 , C12Q1/6834 , C12Q1/6874 , C12Q2565/543 , C12Q2535/101 , C12Q2523/113 , C12Q2523/107 , C12Q2521/331 , C12Q2521/307
摘要： The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5' end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
摘要翻译：本发明涉及产生用于核酸测序反应的模板的方法，其包括：提供至少一个双链核酸分子，其中双链核酸分子的两条链在5'末端连接到固体支持物上切割双链核酸分子的一条或两条链，并使切割的链进行变性条件以除去未连接到固体支持物上的部分切割的链，从而产生部分或基本上用于核酸测序反应的单链模板。

3. 发明申请

WO2004018497A3 MODIFIED NUCLEOTIDES FOR POLYNUCLEOTIDE SEQUENCING 审中-公开
标题翻译：用于多核苷酸序列的修饰核苷酸
公开(公告)号：WO2004018497A3
公开(公告)日：2004-06-17
申请号：PCT/GB0303686
申请日：2003-08-22
申请人： SOLEXA LTD , MILTON JOHN , WU XIAOLIN , SMITH MARK , BRENNAN JOSEPH , BARNES COLIN , LIU XIAOHAI , RUEDIGER SILKE
发明人： MILTON JOHN , WU XIAOLIN , SMITH MARK , BRENNAN JOSEPH , BARNES COLIN , LIU XIAOHAI , RUEDIGER SILKE
IPC分类号： C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C12Q1/68 , G01N33/53
CPC分类号： C12Q1/6869 , C07H1/00 , C07H19/06 , C07H19/10 , C07H19/14 , C07H19/16 , C07H19/20 , C07H21/00 , C12Q2525/186 , C12Q2535/113 , Y02P20/55 , C12Q2525/197
摘要： The invention provides modified nucleotide or nucleoside molecule comprising a purine or pyrimidine base and a ribose or deoxyribose sugar moiety having a removable 3'-OH blocking group covalently attached thereto, such that the 3' carbon atom has attached a group of the structure -O-Z wherein Z is any of -C(R')2-O-R", -C(R')2-N(R")2,-C(R')2-N(H)R", -C(R')2-S-R" and -C(R')2-F, wherein each R" is or is part of a removable protecting group; each R' is independently a hydrogen atom, an alkyl, substituted alkyl, arylalkyl, alkenyl, alkynyl, aryl, heteroaryl, heterocyclic, acyl, cyano, alkoxy, aryloxy, heteroaryloxy or amido group, or a detectable label attached through a linking group; or (R')2 represents an alkylidene group of formula =C(R''')2 wherein each R''' may be the same or different and is selected from the group comprising hydrogen and halogen atoms and alkyl groups; and wherein said molecule may be reacted to yield an intermediate in which each R" is exchanged for H or, where Z is -C(R')2-F, the F is exchanged for OH, SH or NH2, preferably OH, which intermediate dissociates under aqueous conditions to afford a molecule with a free 3'OH; with the proviso that where Z is -C(R')2-S-R", both R' groups are not H.
摘要翻译：本发明提供了修饰的核苷酸或核苷分子，其包含嘌呤或嘧啶碱基和核糖或脱氧核糖糖部分，其具有共价连接到其上的可移除的3'-OH封闭基团，使得3'碳原子连接有一组结构-OZ 其中Z是-C（R'）2 -OR“，-C（R'）2 -N（R”）2，-C（R'）2 -N（H）R“， - C '）2-SR“和-C（R'）2 -F，其中每个R”是或是可除去保护基的一部分;每个R'独立地是氢原子，烷基，取代的烷基，芳基烷基，烯基，炔基，芳基，杂芳基，杂环，酰基，氰基，烷氧基，芳氧基，杂芳氧基或酰胺基，或通过连接基团连接的可检测标记;或（R'）2表示式= C（R“ ）2，其中每个R“可以相同或不同，并且选自氢和卤素原子和烷基;并且其中所述分子可以反应以产生其中每个R”被交换为H或，Z 是-C（R'）2 -F，F被交换成OH，SH或NH 2，优选OH，该中间体在水溶液条件下解离以得到具有游离3'OH的分子; 条件是其中Z是-C（R'）2 -S-R“，两个R'基团不是H.

4. 发明申请

WO0204488A2 NUCLEIC ACID BINDING POLYPEPTIDES 审中-公开
标题翻译：核酸结合多肽
公开(公告)号：WO0204488A2
公开(公告)日：2002-01-17
申请号：PCT/GB0103130
申请日：2001-07-12
申请人： SANGAMO BIOSCIENCES INC , UNIV CAMBRIDGE TECH , CHOO YEN , ISALAN MARK , LIU XIAOHAI , PATEL SACHIN , BALASUBRAMANIAN SHANKAR
发明人： CHOO YEN , ISALAN MARK , LIU XIAOHAI , PATEL SACHIN , BALASUBRAMANIAN SHANKAR
IPC分类号： C07K14/47 , C12N15/12 , C07K14/00
CPC分类号： A61K38/1709 , C07K14/4702 , C07K2319/00
摘要： We describe the use of a nucleic acid binding polypeptide capable of binding to one or more of telomeric, G-quadruplex, or G-quartet nucleic acid as an inhibitor of enzymatic activity, including a telomerase activity, a polymerase activity, an integrase activity and a gp120 activity. Telomerase assays as well as methods of identifying molecules capable of interacting with telomeric, G-quadruplex, or G-quartet nucleic acid are also described.
摘要翻译：我们描述了能够结合一种或多种端粒，G-四联体或G-四联体核酸作为酶活性抑制剂的核酸结合多肽的用途，所述酶活性包括端粒酶活性，聚合酶活性，整合酶活性和一个gp120活动。还描述了端粒酶测定以及鉴定能够与端粒，G-四联体或G-四联体核酸相互作用的分子的方法。

5. 发明申请

WO2007020457A3 LABELLED MODIFIED GUANINE- CONTAINING NUCLEOSIDES AND NUCLEOTIDES COMPRISING A FLUOROPHORE ATTACHED TO THE BASE THROUGH A LINKING GROUP COMPRISING A POLYETHYLENE GLYCOL SPACING GROUP AND METHODS FOR THEIR USE 审中-公开
标题翻译：包含修饰的含有核苷酸的核苷酸和包含通过包含聚乙烯甘油酯间隔基团的连接基团连接到基底上的荧光体的核苷酸及其使用方法
公开(公告)号：WO2007020457A3
公开(公告)日：2007-10-25
申请号：PCT/GB2006003095
申请日：2006-08-18
申请人： SOLEXA LTD , LIU XIAOHAI , MILTON JOHN
发明人： LIU XIAOHAI , MILTON JOHN
IPC分类号： C12Q1/68 , C07H19/16 , C07H19/20 , C07H21/00
CPC分类号： C12Q1/686 , C12Q2525/113
摘要： Modified guanine-containing nucleosides and nucleotides, in particular fluorescently labelled guanine-containing nucleosides and nucleotides, which exhibit reduced quenching effects, and hence enhanced brightness of the fluorophore are described.
摘要翻译：描述了改进的含鸟嘌呤的核苷和核苷酸，特别是荧光标记的含有鸟嘌呤的核苷和核苷酸，其表现出降低的淬灭效果，并因此提高了荧光团的亮度。

6. 发明专利

IL279644D0 Nucleosides and nucleotides with 3'-hydroxy blocking groups and their use in polynucleotide sequencing methods 未知
公开(公告)号：IL279644D0
公开(公告)日：2021-03-01
申请号：IL27964420
申请日：2020-12-21
申请人： ILLUMINA CAMBRIDGE LTD , CRESSINA ELENA , FRANCAIS ANTOINE , LIU XIAOHAI , WU XIAOLIN , CULLEY ADAM , MARIANI ANGELICA
发明人： CRESSINA ELENA , FRANCAIS ANTOINE , LIU XIAOHAI , WU XIAOLIN , CULLEY ADAM , MARIANI ANGELICA
IPC分类号： C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C12Q1/6869
摘要： Embodiments of the present disclosure relate to sequencing methods using nucleotide molecules with a 3′ AOM blocking group, which can provide reduced phasing and pre-phasing values. Also provided herein are kits related to such sequencing methods.

7. 发明专利

IL271446D0 Compositions and methods for chemical cleavage and deprotection of surface-bound oligonucleotides 未知
公开(公告)号：IL271446D0
公开(公告)日：2020-01-30
申请号：IL27144619
申请日：2019-12-15
申请人： ILLUMINA INC , ILLUMINA CAMBRIDGE LTD , GEORGE WAYNE N , BEIERLE JOHN M , SHIEH PEYTON , SMITH RANDALL , LIU XIAOHAI , WU XIAOLIN , LAWRENCE ELLIOT JOHN , MAO JIE
发明人： GEORGE WAYNE N , BEIERLE JOHN M , SHIEH PEYTON , SMITH RANDALL , LIU XIAOHAI , WU XIAOLIN , LAWRENCE ELLIOT JOHN , MAO JIE
IPC分类号： C07H20060101
摘要： Embodiments of the present disclosure relate to methods of preparation of templates for polynucleotide sequencing. In particular, the disclosure relates to linearization of clustered polynucleotides in preparation for sequencing by cleavage of one or more first strands of double-stranded polynucleotides immobilized on a solid support by a transition metal complex, for example, a palladium complex or a nickel complex. Further disclosure relate to linearization of clustered polynucleotides by cleaving one or more second strands of double double-stranded polynucleotides immobilized on a solid support comprising azobenzene linker by Na2S2O4. Nucleotides and oligonucleotides comprising a 3′ phosphate moiety blocking group, and methods of removing the same using a fluoride reagent are also disclosed.

8. 发明专利

IL258256A Polymethine compounds and their use as fluorescent labels 未知
公开(公告)号：IL258256A
公开(公告)日：2020-10-29
申请号：IL25825618
申请日：2018-03-20
申请人： LIU XIAOHAI , FRANCAIS ANTOINE , ILLUMINA CAMBRIDGE LTD
发明人： LIU XIAOHAI , FRANCAIS ANTOINE
IPC分类号： C09B23/02 , C09B23/06 , G01N33/58
摘要： The present disclosure relates to new compounds and their use as fluorescent labels. The compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications.

9. 发明专利

IL286668D0 Kits and flow cells 未知
公开(公告)号：IL286668D0
公开(公告)日：2021-12-01
申请号：IL28666821
申请日：2021-09-24
申请人： ILLUMINA CAMBRIDGE LTD , ILLUMINA INC , GEORGE WAYNE N , MATHER BRIAN D , BROWN ANDREW A , LAFRANCONI PIETRO GATTI , ROGERT BACIGALUPO MARIA CANDELARIA , FRANCAIS ANTOINE , LIU XIAOHAI
发明人： GEORGE WAYNE N , MATHER BRIAN D , BROWN ANDREW A , LAFRANCONI PIETRO GATTI , ROGERT BACIGALUPO MARIA CANDELARIA , FRANCAIS ANTOINE , LIU XIAOHAI
摘要： An example of a kit includes a flow cell and a cleavage mix. An example flow cell includes a substrate; a catalytic polymeric hydrogel on the substrate, the catalytic polymeric hydrogel including a deblocking catalyst; and an amplification primer attached to the catalytic polymeric hydrogel. The deblocking catalyst accelerates cleavage of a blocking group of a 3′ OH blocked nucleotide introduced to the flow cell and incorporated into a template strand attached to the amplification primer. An example of the cleavage mix includes a component to initiate cleavage of the blocking group.

10. 发明专利

IL250498A Modified nucleotide linkers 未知
公开(公告)号：IL250498A
公开(公告)日：2021-03-25
申请号：IL25049817
申请日：2017-02-07
申请人： ILLUMINA CAMBRIDGE LTD , WU XIAOLIN , LIU XIAOHAI
发明人： WU XIAOLIN , LIU XIAOHAI
IPC分类号： C07H1/00 , C07H19/14 , C12Q1/6869 , G01N21/64
摘要： Some embodiments of the present application relate to novel modified nucleotide linkers for increasing the efficiency of nucleotide incorporation in Sequencing by Synthesis applications. Methods of preparing these modified nucleotide linkers are also provided herewith.

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