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1. 发明申请

WO2015051319A2 TAILORED OILS 审中-公开
标题翻译：定制油
公开(公告)号：WO2015051319A2
公开(公告)日：2015-04-09
申请号：PCT/US2014/059161
申请日：2014-10-03
申请人： SOLAZYME, INC.
发明人： FRANKLIN, Scott , SOMANCHI, Aravind , RUDENKO, George , BHAT, Riyaz , ZHAO, Xinhua , MOSELEY, Jeffrey L.
IPC分类号： C12P7/64
CPC分类号： C12P7/64 , C11C1/002 , C11D9/00 , C12P7/6409 , C12Y203/01041 , C12Y301/02014
摘要： Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desaturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.
摘要翻译：使用重组DNA技术来生产产油甘油三酯油的含油重组细胞，所述甘油三酯油具有期望的脂肪酸谱和区域特异性或立体特异性谱。操作的基因包括编码硬脂酰-ACP去饱和酶，Δ12脂肪酸去饱和酶，酰基-ACP硫酯酶，酮脂酰-ACP合酶和溶血磷脂酸酰基转移酶的基因。所产生的油可具有增强的氧化或热稳定性，或可用作油炸油，起酥油，卷入起酥油，脂肪回火，替代可可脂，作为润滑剂或作为各种化学工艺的原料。脂肪酸谱可以在中链谱上富集，或者油可以富含饱和 - 不饱和饱和型甘油三酯。

2. 发明申请

WO2016014968A1 VARIANT THIOESTERASES AND METHODS OF USE 审中-公开
标题翻译：各种各样的药物和使用方法
公开(公告)号：WO2016014968A1
公开(公告)日：2016-01-28
申请号：PCT/US2015/042044
申请日：2015-07-24
申请人： SOLAZYME, INC. , BHAT, Riyaz
发明人： BHAT, Riyaz , DAVIS, David , FRANKLIN, Scott , MOSELEY, Jeffrey L.
IPC分类号： C12N9/16 , C12N15/82 , C12P7/64
CPC分类号： C12P7/6463 , C12N9/16 , C12N15/8247 , C12Y301/02014
摘要： The present invention relates to variant thioesterases and their use in plants, e.g., to increase enzymatic activity and to promote increased production of mid-chain length fatty acids (e.g., 8 to 14 carbons) and at desired ratios. Further disclosed herein are methods of manufacturing renewable chemicals through the manufacture of novel triglyceride oils followed by chemical modification of the oils. Oils containing fatty acid chain lengths of C8, C10, C12 or C14 are also disclosed and are useful as feedstocks in the methods described herein.
摘要翻译：本发明涉及变体硫酯酶及其在植物中的用途，例如增加酶活性并促进中链长度脂肪酸（例如8至14个碳）的增加和所需比例的生产。本文进一步公开的是通过制备新型甘油三酯油然后化学改性油来制造可再生化学品的方法。还公开了含有C8，C10，C12或C14脂肪酸链长度的油，并且可用作本文所述方法中的原料。

3. 发明申请

WO2013082186A2 GENETICALLY ENGINEERED MICROBIAL STRAINS INCLUDING PROTOTHECA LIPID PATHWAY GENES 审中-公开
标题翻译：遗传工程微生物菌株，包括原生动物路线基因
公开(公告)号：WO2013082186A2
公开(公告)日：2013-06-06
申请号：PCT/US2012/066893
申请日：2012-11-28
申请人： SOLAZYME, INC. , FRANKLIN, Scott , BRUBAKER, Shane , RUDENKO, George N. , MOSELEY, Jeffrey L. , ZHAO, Xinhua , HUYNH, Tina T. , BHAT, Riyaz , SHOA-AZAR, Matthew , NGUYEN, Trung H. , ESPINA, Karen , SOMANCHI, Aravind
发明人： FRANKLIN, Scott , BRUBAKER, Shane , RUDENKO, George N. , MOSELEY, Jeffrey L. , ZHAO, Xinhua , HUYNH, Tina T. , SHOA-AZAR, Matthew , NGUYEN, Trung H. , ESPINA, Karen , SOMANCHI, Aravind
IPC分类号： C12N9/00
CPC分类号： A61K39/0011 , C07K14/405 , C12N5/0634 , C12N9/00 , C12N9/0071 , C12N9/1029 , C12N9/16 , C12N15/79 , C12N15/8247 , C12P7/6445 , C12P7/6463 , C12Y114/19002 , C12Y114/19006 , C12Y203/01179 , C12Y301/02014 , Y02P20/52
摘要： Genetically engineered microbial, e.g., Prototheca, cells provide microbial oil useful as a food additive and a source of renewable fuels and industrial chemicals.
摘要翻译：基因工程微生物，例如Prototheca，细胞提供可用作食品添加剂的微生物油和可再生燃料和工业化学品的来源。

4. 发明公开

EP3052636A2 TAILORED OILS 审中-公开
标题翻译：定制油
公开(公告)号：EP3052636A2
公开(公告)日：2016-08-10
申请号：EP14789692.2
申请日：2014-10-03
申请人： Solazyme, Inc.
发明人： FRANKLIN, Scott , SOMANCHI, Aravind , RUDENKO, George , BHAT, Riyaz , ZHAO, Xinhua , MOSELEY, Jeffrey L.
IPC分类号： C12P7/64 , C12N15/63
CPC分类号： C12P7/64 , C11C1/002 , C11D9/00 , C12P7/6409 , C12Y203/01041 , C12Y301/02014
摘要： Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desaturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.
摘要翻译：使用重组DNA技术来产生产油甘油三酯油的含油重组细胞，所述甘油三酯油具有期望的脂肪酸谱和区域特异性或立体特异性谱。操作的基因包括编码硬脂酰-ACP去饱和酶，Δ12脂肪酸去饱和酶，酰基-ACP硫酯酶，酮脂酰-ACP合酶和溶血磷脂酸酰基转移酶的基因。所产生的油可具有增强的氧化或热稳定性，或可用作油炸油，起酥油，卷入起酥油，脂肪回火，替代可可脂，作为润滑剂或作为各种化学工艺的原料。脂肪酸谱可以在中链谱中富集，或者油可以富含饱和 - 不饱和饱和类型的甘油三酯。

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