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1. 发明专利

JP2518607B2 失效
公开(公告)号：JP2518607B2
公开(公告)日：1996-07-24
申请号：JP19008685
申请日：1985-08-29
申请人： SNOW BRAND MILK PROD CO LTD
发明人： NIIMOTO YOJI , KAWAKAMI HIROSHI , DOSEMARI SHUNICHI
IPC分类号： G01N33/569 , A61K39/145 , A61K39/395 , C07K14/005 , C07K14/195 , C07K16/00 , C07K16/10 , C12N15/00 , C12N15/02 , C12N15/09 , C12P21/00 , C12P21/08 , C12R1/91 , G01N33/577

2. 发明专利

JPH05124980A AGING PREVENTING AGENT 失效
公开(公告)号：JPH05124980A
公开(公告)日：1993-05-21
申请号：JP31166291
申请日：1991-10-30
申请人： SNOW BRAND MILK PROD CO LTD
发明人： NIIMOTO YOJI , DOSEMARI SHUNICHI
IPC分类号： A23L1/30 , A23L2/00 , A23L2/52 , A61K8/00 , A61K8/64 , A61K8/66 , A61K8/67 , A61K8/96 , A61K38/44 , A61P39/06 , A61P43/00 , A61Q19/00 , A61Q19/08
摘要： PURPOSE:To obtain an aging preventing agent consisting of a peroxidase and capable of prolonging human life and preventing aging by suppressing in vivo production of peroxilipids causing aging. CONSTITUTION:The objective aging preventing agent is obtained by blending a peroxidase (preferably lactoperoxidase using cow milk as a feed source), as an active ingredient, preferably at a ratio of 0.1-5wt.%. This agent is preferably combinedly used with vitamin E as an antioxidant and/or lactoferrin. Furthermore, iron ion concentration in the aging agent is kept preferably

3. 发明专利

JPH03127994A HUMAN-TYPE DIPHTHERIA ANTITOXIN MONOCLONAL ANTIBODY AND HYBRIDOMA SECRETING THE SAME 失效
公开(公告)号：JPH03127994A
公开(公告)日：1991-05-31
申请号：JP26343389
申请日：1989-10-09
申请人： SNOW BRAND MILK PROD CO LTD
发明人： NIIMOTO YOJI , SATO KAORU , DOSEMARI SHUNICHI
IPC分类号： G01N33/569 , A61K39/395 , C12N5/10 , C12N5/28 , C12N15/02 , C12N15/08 , C12P21/08 , C12R1/91 , G01N33/577
摘要： PURPOSE:To provide an IgM class human-type monoclonal antibody exhibiting antigen-recognition property to diphtheria toxin and utilizable for the remedy of diphtheria infection and neutralization, extraction and determination of the toxin. CONSTITUTION:A B-lymphoblast-like cell(BLC) transformed by Epstein-Barr virus(EBV) is used as an antibody-secretion cell since a B-cell secreting diphtheria antitoxin antibody is practically unavailable. The BLC is fused with a mother stock and the resultant fused cell is used as the cell for secreting human- type diphtheria toxin antibody. The cell fusion of the mother strain and BLC is carried out as follows. The mother strain is mixed with BLC at a ratio of 1:10-10:1 and fused with each other using e.g. polyethylene glycol. An IgM class human-type monoclonal antibody can be produced by this process.

4. 发明专利

JPH01217266A NON-SPECIFIC ABSORPTION PREVENTING AGENT AND METHOD 失效
公开(公告)号：JPH01217266A
公开(公告)日：1989-08-30
申请号：JP4215488
申请日：1988-02-26
申请人： SNOW BRAND MILK PROD CO LTD
发明人： DOSEMARI SHUNICHI , NIIMOTO YOJI
IPC分类号： G01N33/531 , G01N33/543
摘要： PURPOSE:To obtain a non-specific adsorption preventing agent having transparency and generating no browning by sterilization treatment, by using milk protein as an effective component and combining the same with a buffer solution based on one or more kind of a component selected from an org. acid and an org. acid salt. CONSTITUTION:Casein, a whey protein concentrate (WPC) and one of milk proteins selected from defatted powdered milk and skim milk or a combination of them are dissolved in a buffer solution based on one more kind of a component selected from an org. acid and an org. acid sat. As the org. acid, citric acid, maleic acid, malic acid, succinic acid, malonic acid or the like are used. The pH of the buffer solution is pref. 5.3-7.0 and the ratio of milk solid concetration (%)/org. acid concentration (mM) is pref. 0.1 or less. This non- specific adsorption preventing agent has transparency and generates no browning by sterilization and has excellent blocking effect in immunoassay.

5. 发明专利

JPS62294620A METHOD FOR PREPARING CYTOSTATIC FACTOR 失效
公开(公告)号：JPS62294620A
公开(公告)日：1987-12-22
申请号：JP13943886
申请日：1986-06-16
申请人： SNOW BRAND MILK PROD CO LTD
发明人： NIIMOTO YOJI , DOSEMARI SHUNICHI , MURAKAMI HIRONORI
IPC分类号： A61K35/12 , A61K35/13 , A61K38/00 , A61K38/16 , A61K38/17 , A61P43/00 , C07K14/435 , C12P21/00 , C12R1/91
摘要： PURPOSE:To prepare the titled factor, by cultivating an animal cancerous cell strain to give a culture animal cell, extracting the cell, separating and collecting a protein component. CONSTITUTION:An animal cancerous cell stain (e.g. human cancerous cell stain, etc.) is cultivated to give a culture animal cell, which is extracted. In the operation, the extraction is preferably carried out at about 4 deg.C low temperature. A protein component is separated and collected from the prepared extract by combination of salting-out, various kinds of chromatography (e.g. gel filtration, ion exchange chromatography, etc.). The obtained component is a uniform group in terms of protein chemistry and it can be said as a protein substance because it deactivates by heating, loses pH activity in an acidic range, shows 280nm absorption in adsorbance and protein dyeing is possible. The component is added to a medium wherein cells (e.g. cancerous cell strain, etc.) are multiplying in vitro and can be used as a cytostatic factor.

6. 发明专利

JPS62244392A PRODUCTION OF ANTIBODY USING HYBRIDOMA 失效
公开(公告)号：JPS62244392A
公开(公告)日：1987-10-24
申请号：JP8514286
申请日：1986-04-15
申请人： SNOW BRAND MILK PROD CO LTD
发明人： NIIMOTO YOJI , DOSEMARI SHUNICHI , MURAKAMI HIRONORI
IPC分类号： C12N15/02 , C12N5/00 , C12N5/10 , C12N15/00 , C12P21/00 , C12P21/08 , C12R1/91
摘要： PURPOSE:To obtain an antibody, having two kinds of heavy chains and light chains in the same molecule and useful for clinical tests or treatment, by cultivating a hybridoma prepared by subjecting specific cells to cell fusion. CONSTITUTION:Antibody-producing cells, capable of producing two kinds of antibodies and selected from the group of human myelomatous cells, human B lymphoblastic cells and human normal lymphocytes are subjected to cell fusion in a solution of 40-60wt% polyethylene glycol having 1,000-6,000 molecular weight to give a hybridoma, which is then cultivated in a culture medium prepared by adding 5-20wt% blood serum, e.g. bovine blood serum, etc., to Dulbecco's modified minimum essential culture medium, etc., in air containing 5% CO2 gas in an atmosphere at >=90% humidity and 37 deg.C. Antibodies having two kinds of heavy chains e.g. human alpha-chains or mu-chains, and light chains, e.g. human k-chains or lambda-chains, in the same molecule are produced in the above-mentioned culture.

7. 发明专利

JPS6226237A PRODUCTION OF POLYCLONAL HUMAN IMMUNOGLOBULIN OF SINGLE CLASS 失效
公开(公告)号：JPS6226237A
公开(公告)日：1987-02-04
申请号：JP16515585
申请日：1985-07-26
申请人： SNOW BRAND MILK PROD CO LTD
发明人： NIIMOTO YOJI , KAWAKAMI HIROSHI , DOSEMARI SHUNICHI
IPC分类号： C12P21/00 , A61K38/21 , A61K39/395 , C12N15/00 , C12N15/02 , C12P21/08 , C12Q1/00 , C12R1/91
摘要： PURPOSE:To obtain the titled immunoglobulin useful for remedying immunological incompetence, by separating a cell producing a polyclonal human immunoglobulin from a cell group producing a human immunoglobulin and cultivating it. CONSTITUTION:A cell group (e.g., human lymphoblast transformed with Epstein- Barr virus or hybridoma between human normal lymphocyte and human or mouse bone marrow cell) producing a human immunoglobulin is brought into contact with a solid phase linked to an anti-human immunoglobulin antibody, a cell group to produce a human immunoglobulin of a specific class is selectively adsorbed on the solid phase, separated and cultivated, to give a polyclonal human immunoglobulin (e.g., human IgA, human IgG, or human IgM) of a single class. The polyclonal human immunoglobulin shows improved antibody effects on unspecific bacteria and viruses and is useful for remedying immunological incompetence, etc.

8. 发明专利

JPH0557997B2 失效
公开(公告)号：JPH0557997B2
公开(公告)日：1993-08-25
申请号：JP2241384
申请日：1984-02-09
申请人： SNOW BRAND MILK PROD CO LTD
发明人： KAWAKAMI HIROSHI , NIIMOTO YOJI , DOSEMARI SHUNICHI , AHIKO KENKICHI
IPC分类号： A61K39/395 , A61K35/20 , A61K38/16 , C07K1/16 , C07K1/22 , C07K14/435 , C07K16/00 , C12P21/08 , C12R1/91 , C07K3/20 , A61K37/14 , C07K15/06

9. 发明专利

JPH0542269B2 失效
公开(公告)号：JPH0542269B2
公开(公告)日：1993-06-28
申请号：JP1885286
申请日：1986-01-30
申请人： SNOW BRAND MILK PROD CO LTD
发明人： NIIMOTO YOJI , DOSEMARI SHUNICHI , MURAKAMI HIRONORI
IPC分类号： G01N33/531 , A61K39/395 , C12N15/02 , C12P21/00 , C12P21/08 , C12R1/91

10. 发明专利

JPH04108390A METHOD FOR TRANSDUCING EXOGENOTE INTO ANIMAL CELL 失效
公开(公告)号：JPH04108390A
公开(公告)日：1992-04-09
申请号：JP22761990
申请日：1990-08-29
申请人： SNOW BRAND MILK PROD CO LTD
发明人： NIIMOTO YOJI , KAWASAKI ISAHIRO , SATO KAORU , DOSEMARI SHUNICHI
IPC分类号： C12N15/09 , C07K1/113 , C07K1/22 , C07K14/00
摘要： PURPOSE:To enable transduction of an exogenote without applying stress to animals cells by adding a complex of the objective gene to be transduced and lactoferrin into a culture solution of the animal cells, allowing the resultant mixture to stand and incorporating the complex into the cells. CONSTITUTION:Modified lactoferrin or lactoferrin is dissolved in a previously prepared buffer solution and a prescribed amount of a gene is further added to gently stir and mix both. The resultant solution is then allowed to stand at a prescribed temperature for a prescribed time to produce a DNA lactoferrin complex. A buffer solution containing a prescribed amount of the resultant DNA lactoferrin complex is subsequently added into a culture solution in which cells to be transduced are precultured and culturing is continued for a prescribed time. The DNA sticks through the lactoferrin to cell surfaces and incorporated into the cells. If the gene to be transduced is marked with a selective marker, the cells into which the gene is transduced can readily be selected after completing the culturing. After the transduction of the DNA, the culture solution can be exchanged with a culture medium containing a chemical for selection added thereto to carry out culturing for a prescribed period. Thereby, the objective survival cells as cells in which the gene is transduced are obtained.

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