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1. 发明申请

WO2005062725A3 METHOD FOR ENRICHMENT/SEPARATION OF PROTEIN OR PEPTIDE 审中-公开
标题翻译：富集/分离蛋白质或肽的方法
公开(公告)号：WO2005062725A3
公开(公告)日：2005-11-17
申请号：PCT/JP2004019677
申请日：2004-12-21
申请人： SHIMADZU CORP , WATANABE MAKOTO , MATSUO EIICHI , TODA CHIKAKO , NISHIMURA OSAMA
发明人： WATANABE MAKOTO , MATSUO EIICHI , TODA CHIKAKO , NISHIMURA OSAMA
IPC分类号： G01N30/88 , B01J20/281 , C07K1/13 , C07K1/14 , C07K1/16 , C07K1/24 , G01N30/06 , G01N30/26 , G01N30/56 , G01N30/80 , G01N30/84
CPC分类号： C07K1/24
摘要： The present invention finds a media that can highly selectively retain proteins or peptides to be enriched/separated, as well as provides a method for selectively enriching/separating proteins or peptides using such a media. A method for enrichment/separation of a protein or a peptide, comprising separating a protein or a peptide containing an amino acid residue with a pi electron-containing group by using a media with a pi electron-containing group. Preferably, the amino acid residue is a tryptophan residue or a tryptophan residue modified with a sulfenyl compound, and the media is a media with phenyl group.
摘要翻译：本发明发现了可高度选择性地保留待富集/分离的蛋白质或肽的培养基，并提供了使用此类培养基选择性富集/分离蛋白质或肽的方法。用于富集/分离蛋白质或肽的方法，包括通过使用具有含π电子基团的介质将蛋白质或含有氨基酸残基的肽与含π电子基团分离。优选地，氨基酸残基是用亚硫酰基化合物修饰的色氨酸残基或色氨酸残基，并且该培养基是具有苯基的培养基。

2. 发明专利

AU2003236368A1 METHOD OF ELIMINATING PHOSPHATE GROUP OF PEPTIDE AND METHOD OF ANALYZING PEPTIDE 未知
公开(公告)号：AU2003236368A1
公开(公告)日：2004-09-06
申请号：AU2003236368
申请日：2003-04-03
申请人： SHIMADZU CORP
发明人： KUYAMA HIROKI , TODA CHIKAKO , NISHIMURA OSAMU
IPC分类号： C07K1/113 , C07K7/06 , C07K7/08 , G01N33/68 , G01N27/62
摘要： The present invention provides a method for eliminating phosphate groups of peptides that relies not on enzymes but on a chemical treatment, and an effective method for peptide analysis that uses such a method. A method for eliminating a phosphate group of a peptide, the method comprising the use of a reagent containing at least one selected from the group consisting of hydrogen fluoride, hydrofluoric acid, and a hydrogen fluoride-containing compound, and a method for peptide analysis that uses such a method. As the hydrogen fluoride-containing compound, hydrogen fluoride-pyridine is preferably used. The elimination of the phosphate group from a peptide may be carried out so that the total amount of the hydrogen fluoride, hydrogen fluoride in the hydrofluoric acid, and hydrogen fluoride in the hydrogen fluoride-containing compound contain in the reagent is 10 to 100wt% with respect to the reagent, and the temperature for the elimination reaction is -10 to 50 DEG C. Further, the peptide analysis may be performed by mass spectrometry with the use of MALDI-TOFMS.

3. 发明公开

EP1739419A4 METHOD OF MEASURING SUBSTANCE BY MASS SPECTROMETRY AND KIT THEREFOR 审中-公开
标题翻译：测量物质的通过质谱分析法及其试剂盒方法
公开(公告)号：EP1739419A4
公开(公告)日：2008-12-03
申请号：EP05728584
申请日：2005-04-01
申请人： SHIMADZU CORP
发明人： MATSUO EIICHI , WATANABE MAKOTO , OJIMA NORIYUKI , TODA CHIKAKO , KUYAMA HIROKI , NISHIMURA OSAMU
IPC分类号： G01N27/64 , G01N27/62 , H01J49/16
CPC分类号： G01N33/6851 , G01N33/6842 , H01J49/16
摘要： A method of measuring a substance by mass spectrometry; and a kit therefor. In particular, there are provided a method of measuring a hydrophobic peptide by a MALDI mass spectrometer; a method of selectively measuring a specified substance from a mixture by MALDI mass spectrometry; and a method of quantitative analysis for protein and a kit therefor. Further, there is provided a method of measuring a peptide by a MALDI mass spectrometer with the use of alpha-cyano-3-hydroxycinnamic acid or 3-hydroxy-4-nitrobenzoic acid as a matrix. Still further, there is provided a method of mass spectrometry, comprising specifically ionizing a given substance contained in a mixture of given substance to be measured and other substances with the use of a matrix capable of ionizing the given substance more easily than the other substances and measuring the given substance selectively from the mixture. Furthermore, there are provided a relevant method of quantitative analysis for protein and kit therefor.

4. 发明公开

EP1595889A4 METHOD OF ELIMINATING PHOSPHATE GROUP OF PEPTIDE AND METHOD OF ANALYZING PEPTIDE 审中-公开
标题翻译：方法肽的磷酸基肽分析消除和方法
公开(公告)号：EP1595889A4
公开(公告)日：2008-05-28
申请号：EP03815854
申请日：2003-04-03
申请人： SHIMADZU CORP
发明人： KUYAMA HIROKI , TODA CHIKAKO , NISHIMURA OSAMU
IPC分类号： C07K1/113 , C07K7/06 , C07K7/08 , G01N33/68 , G01N27/62
CPC分类号： G01N33/6848 , C07K1/113 , C07K7/06 , C07K7/08 , G01N33/6842 , G01N33/6851 , Y10T436/25125
摘要： It is intended to provide a method of eliminating a phosphate group of a peptide by a chemical treatment without resort to any enzymes and a method of efficiently analyzing a peptide by using the above method. Namely, a method of eliminating a phosphate group of a peptide by using a reagent containing at lease one member selected from the group consisting of hydrogen fluoride, hydrofluoric acid and a hydrogen fluoride-containing compound, and a method of analyzing a peptide by using this method. As the hydrogen fluoride-containing compound, it is preferable to employ hydrogen fluoride-pyridine. The elimination of a phosphate group of a peptide is carried out by controlling the total amount of hydrogen fluoride in hydrogen fluoride, hydrofluoric acid and the hydrogen fluoride-containing compound contained in the reagent to 10 to 100% by weight at a reaction temperature of -10 to 50°C. The analysis of a peptide is carried out preferably by mass spectroscopy with the use of MALDI-TOFMS.

5. 发明公开

EP1700114A4 METHOD FOR ENRICHMENT/SEPARATION OF PROTEIN OR PEPTIDE 审中-公开
标题翻译： PROCESS FOR富集/分离的蛋白质或肽
公开(公告)号：EP1700114A4
公开(公告)日：2007-07-11
申请号：EP04808028
申请日：2004-12-21
申请人： SHIMADZU CORP
发明人： WATANABE MAKOTO , MATSUO EIICHI , TODA CHIKAKO , NISHIMURA OSAMA
IPC分类号： G01N30/88 , B01J20/281 , C07K1/13 , C07K1/14 , C07K1/16 , C07K1/24 , G01N30/06 , G01N30/26 , G01N30/56 , G01N30/80 , G01N30/84
CPC分类号： C07K1/24
摘要： The present invention finds a media that can highly selectively retain proteins or peptides to be enriched/separated, as well as provides a method for selectively enriching/separating proteins or peptides using such a media. A method for enrichment/separation of a protein or a peptide, comprising separating a protein or a peptide containing an amino acid residue with a pi electron-containing group by using a media with a pi electron-containing group. Preferably, the amino acid residue is a tryptophan residue or a tryptophan residue modified with a sulfenyl compound, and the media is a media with phenyl group.

6. 发明专利

JP2006010672A Method of selectively measuring specified substance from mixture by maldi mass spectrometry 有权
标题翻译：通过MALDI MASS SPECTROMETRY从混合物中选择性地测量指定物质的方法
公开(公告)号：JP2006010672A
公开(公告)日：2006-01-12
申请号：JP2004368508
申请日：2004-12-20
申请人： Shimadzu Corp , 株式会社島津製作所
发明人： MATSUO HIDEKAZU , TODA CHIKAKO , WATANABE MAKOTO , NISHIMURA TADASHI , OSHIMA NORIYUKI
IPC分类号： G01N27/62 , G01N27/64 , H01J49/04 , H01J49/26
CPC分类号： G01N33/6842
摘要： PROBLEM TO BE SOLVED: To provide a method that can be effectively used for a measured specimen that does not undergo an enriching process and can detect a target substance mainly by mass spectrometry. SOLUTION: In this mass spectrometry method, a specified substance contained in a mixture sample containing the specified substance to be measured and substances other than the specified substance is ionized specifically with the use of a matrix capable of ionizing the specified substance more easily than the substances other than the specified substance, and the specified substance is selectively measured from the mixture. Preferably, the specified substance is peptide labeled by 2-nitrobenzene sulfenyl chloride, and the matrix is nitrobenzene derivative such as hydroxynitrobenzoic acid. The matrix is preferably used with α-cyano-4-hydroxycinnamic acid. COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：提供一种可以有效地用于不经历浓缩过程的测量样品并且可以主要通过质谱法检测目标物质的方法。解决方案：在该质谱法中，使用能够更容易地电离特定物质的基质，特异性地电离含有待测量的特定物质和特定物质的混合物样品中的特定物质比特定物质以外的物质，并且从混合物中选择性地测量指定的物质。优选地，特定的物质是由2-硝基苯亚硫酰氯标记的肽，并且基质是硝基苯衍生物如羟基硝基苯甲酸。该基质优选与α-氰基-4-羟基肉桂酸一起使用。版权所有（C）2006，JPO＆NCIPI

7. 发明专利

JP2005326391A Method for measuring hydrophobic peptide by maldi mass spectrometer 有权
标题翻译：通过MALDI MASS SPECTROMETER测量疏水性肽的方法
公开(公告)号：JP2005326391A
公开(公告)日：2005-11-24
申请号：JP2004368507
申请日：2004-12-20
申请人： Shimadzu Corp , 株式会社島津製作所
发明人： MATSUO HIDEKAZU , WATANABE MAKOTO , OSHIMA NORIYUKI , TODA CHIKAKO , KUYAMA HIROKI , NISHIMURA TADASHI
IPC分类号： G01N27/62 , G01N33/483 , G01N33/68 , H01J49/10
摘要： PROBLEM TO BE SOLVED: To provide a method capable of efficiently ionizing hydrophobic peptides in MALDI-IT type, MALDI-IT-TOF type, and MALDI-FTICR type mass spectrometers.
SOLUTION: Peptides are measured through the use of α-cyano-3-hydroxy cinnamic acid or 3-hydroxy-4-nitrobenzoic acid by a mass spectrometer, having a MALDI (Matrix-Assisted Laser Desorption Ionization) ion source. Peptides, derivatized by 2-nitrobenzene sulfenyl chloride, are preferably measured by a MALDI-IT type, MALDI-IT-TOF type, or MALDI-FTICR type mass spectrometers. When using 3-hydroxy-4-nitrobenzoic acid as a matrix, it is preferable that it be used as a mixed matrix, in combination with α-cyano-4-hydroxy cinnamic acid.
COPYRIGHT: (C)2006,JPO&NCIPI
摘要翻译：要解决的问题：提供能够有效地离子化MALDI-IT型，MALDI-IT-TOF型和MALDI-FTICR型质谱仪中的疏水性肽的方法。解决方案：通过使用具有MALDI（基质辅助激光解吸电离）离子源的质谱仪，通过使用α-氰基-3-羟基肉桂酸或3-羟基-4-硝基苯甲酸来测量肽。由2-硝基苯亚硫酰氯衍生的肽优选通过MALDI-IT型，MALDI-IT-TOF型或MALDI-FTICR型质谱仪测量。当使用3-羟基-4-硝基苯甲酸作为基质时，优选与α-氰基-4-羟基肉桂酸组合使用作为混合基质。版权所有（C）2006，JPO＆NCIPI

8. 发明专利

JP2007063179A Monoclonal antibody to nitrobenzenesulfenyl group-containing tryptophan and antibody column using the antibody 审中-公开
标题翻译：使用抗体的单克隆抗体对含硝基苯乙烯基的含有三聚氰胺和抗体柱
公开(公告)号：JP2007063179A
公开(公告)日：2007-03-15
申请号：JP2005250625
申请日：2005-08-31
申请人： Shimadzu Corp , 株式会社島津製作所
发明人： TODA CHIKAKO , ANDO EIJI , NISHIMURA TADASHI
IPC分类号： C07K16/44 , C07D209/30 , C07K1/22 , C12N5/10 , C12N15/02 , C12P21/08 , G01N30/88
摘要： PROBLEM TO BE SOLVED: To provide a monoclonal antibody specifically recognizing NBS-modified peptide, to provide a hybridoma capable of producing the monoclonal antibody, and to provide methods for producing them, and to provide an antibody column using the monoclonal antibody, and to provide a method for selectively isolating the NBS-modified peptide by using the antibody column. SOLUTION: The monoclonal antibody recognizing an NBS group-bearing tryptophan is provided. The hybridoma cell capable of producing the monoclonal antibody is provided. The method for producing the hybridoma cell involving using an amino acid residue-free and NBS group-containing tryptophan derivative as immunogen is provided. The method for producing the monoclonal antibody comprising making the hybridoma cell produce the monoclonal antibody is provided. The antibody column having the monoclonal antibody is provided. The method for isolating the NBS group-bearing tryptophan-containing peptide by using the antibody column is also provided. COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：待解决的问题：提供特异性识别NBS-修饰的肽的单克隆抗体，以提供能够产生单克隆抗体的杂交瘤，并提供其制备方法，并使用单克隆抗体提供抗体柱，并提供通过使用抗体柱选择性分离NBS修饰的肽的方法。提供了识别含有NBS基团的色氨酸的单克隆抗体。提供能够产生单克隆抗体的杂交瘤细胞。提供了涉及使用无氨基酸残基和含有NBS基团的色氨酸衍生物作为免疫原的杂交瘤细胞的制备方法。提供了包括使杂交瘤细胞产生单克隆抗体的单克隆抗体的制备方法。提供具有单克隆抗体的抗体柱。还提供了通过使用抗体柱分离含有NBS基团的含色氨酸肽的方法。版权所有（C）2007，JPO＆INPIT

9. 发明专利

JP2007183134A Analysis method of physiological substance-containing sample 审中-公开
标题翻译：生理物质含量样品的分析方法
公开(公告)号：JP2007183134A
公开(公告)日：2007-07-19
申请号：JP2006000731
申请日：2006-01-05
申请人： Shimadzu Corp , 株式会社島津製作所
发明人： MATSUO HIDEKAZU , TODA CHIKAKO , NISHIMURA TADASHI
IPC分类号： G01N27/62 , G01N33/483 , G01N33/68
摘要： PROBLEM TO BE SOLVED: To provide an analysis method of a physiological substance-containing sample, capable of enhancing the efficiency and accuracy of the analysis of protein.
SOLUTION: The physiological substance-containing sample is analyzed by a process (i) for preparing the physiological substance-containing samples I and II, a process (ii-A) for modifying the sample I by a heavy reagent and modifying the sample II by a light reagent, a process (ii-B) for modifying the sample I by the light reagent and modifying the sample II by the heavy reagent, a process (iii-A) for mutually mixing the modified samples obtained in the process (ii-A), a process (iii-B) for mutually mixing the modified samples, obtained in the process (ii-B) and a process (iv) for respectively performing the mass analysis of the mixed sample A obtained by the process (iii-A) and the mixed sample B obtained by the process (iii-B) to compare both mass spectra.
COPYRIGHT: (C)2007,JPO&INPIT
摘要翻译：待解决的问题：提供能够提高蛋白质分析的效率和准确性的含有生理物质的样品的分析方法。解决方案：通过用于制备含有生理物质的样品I和II的方法（i）分析含生理物质的样品，通过重试剂修饰样品I的方法（ii-A），并修饰通过轻试剂的样品II，用于通过轻试剂修饰样品I并用重试剂修饰样品II的方法（ii-B），用于将在该方法中获得的改性样品相互混合的方法（iii-A）（ii-A），用于相互混合在方法（ii-B）中获得的改性样品和分别进行通过该方法得到的混合样品A的质量分析的方法（iv）的方法（iii-B）（iii-A）和通过方法（iii-B）获得的混合样品B以比较两个质谱。版权所有（C）2007，JPO＆INPIT

10. 发明专利

JP2005189104A Method for condensing and separating protein or peptide 审中-公开
标题翻译：冷冻和分离蛋白质或肽的方法
公开(公告)号：JP2005189104A
公开(公告)日：2005-07-14
申请号：JP2003430898
申请日：2003-12-25
申请人： Shimadzu Corp , 株式会社島津製作所
发明人： WATANABE MAKOTO , MATSUO HIDEKAZU , TODA CHIKAKO , NISHIMURA TADASHI
IPC分类号： G01N30/88 , B01J20/281 , C07K1/13 , C07K1/14 , C07K1/16 , C07K1/24 , G01N30/06 , G01N30/26 , G01N30/56 , G01N30/80 , G01N30/84
CPC分类号： C07K1/24
摘要： PROBLEM TO BE SOLVED: To provide a method for detecting a carrier, having superior, selective capability of holding proteins or peptides to be condensed and separated, and selectively condensing and separating the proteins or the peptides through the use of the carrier.
SOLUTION: In the protein or peptide condensing and separating method, proteins or peptides containing amino acid residue, having a π-electron containing functional group, are separated through the use of a carrier having a π-electron containing functional group. The amino acid residue is preferably a tryptophan residue or a tryptophan modified with a sulfenyl compound, and the carrier is preferably a carrier having a phenyl group.
COPYRIGHT: (C)2005,JPO&NCIPI

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