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1. 发明专利

AU2007300996B2 Method for deparaffinization of paraffin-embedded specimen and method for analysis of paraffin-embedded specimen 未知
公开(公告)号：AU2007300996B2
公开(公告)日：2013-10-31
申请号：AU2007300996
申请日：2007-09-21
申请人： SHIMADZU CORP
发明人： TOYAMA ATSUHIKO , SATO TAKA-AKI , AOKI YUTAKA , SHIMADA TAKASHI
IPC分类号： G01N1/28 , G01N1/30 , G01N27/62 , G01N33/48
摘要： Disclosed is a deparaffinization method useful for the mass spectrometry imaging of a paraffin-embedded specimen. Specifically disclosed is a method for the deparaffinization of a paraffin-embedded specimen, which comprises the steps of: exposing a specimen comprising a biological sample embedded in paraffin to an organic solvent compatible with paraffin under heated conditions to melt and dissolve the paraffin in the organic solvent, thereby exposing the biological sample; and separating the organic solvent having the paraffin dissolved therein from the exposed biological sample to remove the paraffin from the specimen.

2. 发明专利

SG11202006629TA METHOD FOR IDENTIFYING PROTEINS 未知
公开(公告)号：SG11202006629TA
公开(公告)日：2020-08-28
申请号：SG11202006629T
申请日：2018-01-09
申请人： SHIMADZU CORP
发明人： SONOMURA KAZUHIRO , SATO TAKA-AKI , MATSUDA FUMIHIKO , WANG YI-TING , ISHIHAMA YASUSHI , TSAI CHIA-FENG
IPC分类号： G01N27/62
摘要： Provided is a method for identifying proteins capable of increasing the number of identified proteins contained in a target sample derived from blood. A protein contained in the target sample derived from blood is fragmented, and a protein contained in an having less bias in a quantity ratio of proteins than the target sample is fragmented, and the fragmented proteins are mixed (Steps S101 to S103). In this manner, the mixed sample in which the bias in a quantity ratio of proteins is less than that of the target sample is generated. By performing MS/MS measurement using the generated mixed sample (Step S107), an MS/MS spectrum of a peak derived from a protein contained in a small amount in the target sample can be prevented from being missed. Accordingly, the number of identified proteins contained in the target sample derived from blood can be increased.

3. 发明专利

DK3023777T3 Fremgangsmåde til fremstilling af peptidfragmenter, kit til fremstilling af peptidfragmenter til anvendelse deri, samt analysefremgangsmåde 未知
公开(公告)号：DK3023777T3
公开(公告)日：2018-01-15
申请号：DK13893016
申请日：2013-09-09
申请人： SHIMADZU CORP
发明人： SHIMADA TAKASHI , FUKAO NORIKO , AOKI CHIKAGE , SATO TAKA-AKI
IPC分类号： C07K1/12

4. 发明专利

CA2922372A1 METHOD FOR PREPARING PEPTIDE FRAGMENTS, KIT FOR PREPARING PEPTIDE FRAGMENTS TO BE USED THEREIN, AND ANALYSIS METHOD 未知
公开(公告)号：CA2922372A1
公开(公告)日：2015-03-12
申请号：CA2922372
申请日：2013-09-09
申请人： SHIMADZU CORP
发明人： SHIMADA TAKASHI , FUKAO NORIKO , AOKI CHIKAGE , SATO TAKA-AKI
IPC分类号： G01N27/62 , G01N1/28
摘要： A method for preparing peptide fragments by cleaving a protein using a protease. The method according to the present invention comprises a step for contacting, in a liquid, a porous body (20), in which the substrate protein (25) to be cleaved is immobilized in pores (29), with microparticles (10) which carry the protease (15) immobilized on the surface thereof. In the present invention, the average particle diameter of the microparticles (10) is larger than the average pore diameter of the porous body (20). According to the method of the present invention, the substrate protein (25) that is an antibody can be site-specifically cleaved. By analyzing the peptide fragments that are obtained by the above method by mass spectrometry, etc., the antibody protein can be detected or quantitatively analyzed.

5. 发明公开

EP2755024A4 HYDROPHILIC THIOL PROBE 审中-公开
标题翻译：水解硫醇
公开(公告)号：EP2755024A4
公开(公告)日：2014-12-24
申请号：EP12830678
申请日：2012-08-17
申请人： SHIMADZU CORP
发明人： SHIMADA TAKASHI , SATO TAKA-AKI , TANAKA KOICHI
IPC分类号： G01N27/62
CPC分类号： G01N33/6848 , Y10T436/145555 , Y10T436/147777 , Y10T436/173845 , Y10T436/19 , Y10T436/200833 , Y10T436/24
摘要： The present invention provides a probe that further promotes ionization in proteomic analysis using mass spectrometry, and a high-sensitive mass spectrometry method for a protein using such a probe. Further, the present invention provides an ionization-enhancing probe that can be used even for a protein that has a high degree of hydrophobicity and quickly turns over, and a high-sensitive mass spectrometry method for a protein using such a probe. A thiol probe for a protein, which is represented by the following formula (I): [Chemical Formula 1] wherein R 1 represents a linker group, and R 2 represents a substituted ammonium group or a substituted amino group. A mass spectrometry method for a protein, comprising the steps of: obtaining a modified protein by reacting the thiol probe with a protein to be subjected to mass spectrometry; and subjecting the modified protein to mass spectrometry.
摘要翻译：本发明提供了一种使用质谱法进一步促进蛋白质组学分析中的电离以及使用这种探针的蛋白质的高灵敏度质谱法的探针。此外，本发明提供了即使对于具有高疏水性和快速折叠的蛋白质也可使用的电离增强型探针，以及使用这种探针的蛋白质的高灵敏度质谱法。由下式（I）表示的蛋白质的硫醇探针：[化学式1]其中R 1表示连接基团，R 2表示取代的铵基或取代的氨基。一种蛋白质的质谱法，其特征在于包括以下步骤：通过硫醇探针与待进行质谱分析的蛋白质反应获得修饰的蛋白质; 并对改性蛋白进行质谱分析。

6. 发明专利

CA2922372C METHOD FOR PREPARING PEPTIDE FRAGMENTS, KIT FOR PREPARING PEPTIDE FRAGMENTS TO BE USED THEREIN, AND ANALYSIS METHOD 未知
公开(公告)号：CA2922372C
公开(公告)日：2018-05-15
申请号：CA2922372
申请日：2013-09-09
申请人： SHIMADZU CORP
发明人： SHIMADA TAKASHI , FUKAO NORIKO , AOKI CHIKAGE , SATO TAKA-AKI
IPC分类号： G01N27/62 , G01N1/28
摘要： A method for preparing peptide fragments by cleaving a protein using a protease. The method according to the present invention comprises a step for contacting, in a liquid, a porous body (20), in which the substrate protein (25) to be cleaved is immobilized in pores (29), with microparticles (10) which carry the protease (15) immobilized on the surface thereof. In the present invention, the average particle diameter of the microparticles (10) is larger than the average pore diameter of the porous body (20). According to the method of the present invention, the substrate protein (25) that is an antibody can be site-specifically cleaved. By analying the peptide fragments that are obtained by the above method by mass spectrometry, etc., the antibody protein can be detected or quantitatively analyzed.

7. 发明专利

SG11201601031QA METHOD FOR PREPARING PEPTIDE FRAGMENTS, KIT FOR PREPARING PEPTIDE FRAGMENTS TO BE USED THEREIN, AND ANALYSIS METHOD 未知
公开(公告)号：SG11201601031QA
公开(公告)日：2016-03-30
申请号：SG11201601031Q
申请日：2013-09-09
申请人： SHIMADZU CORP
发明人： SHIMADA TAKASHI , FUKAO NORIKO , AOKI CHIKAGE , SATO TAKA-AKI
IPC分类号： G01N27/62 , G01N1/28
摘要： A method for preparing peptide fragments by cleaving a protein using a protease. The method according to the present invention comprises a step for contacting, in a liquid, a porous body (20), in which the substrate protein (25) to be cleaved is immobilized in pores (29), with microparticles (10) which carry the protease (15) immobilized on the surface thereof. In the present invention, the average particle diameter of the microparticles (10) is larger than the average pore diameter of the porous body (20). According to the method of the present invention, the substrate protein (25) that is an antibody can be site-specifically cleaved. By analyzing the peptide fragments that are obtained by the above method by mass spectrometry, etc., the antibody protein can be detected or quantitatively analyzed.

8. 发明专利

PL3023777T3 Sposób otrzymywania fragmentów peptydowych, zestaw do otrzymywania fragmentów peptydowych przeznaczony do stosowania w nim i sposób analizy 未知
公开(公告)号：PL3023777T3
公开(公告)日：2018-04-30
申请号：PL13893016
申请日：2013-09-09
申请人： SHIMADZU CORP
发明人： SHIMADA TAKASHI , FUKAO NORIKO , AOKI CHIKAGE , SATO TAKA-AKI
IPC分类号： C07K1/12

9. 发明专利

ES2649147T3 Método para preparar fragmentos de péptidos, kit para preparar fragmentos de péptidos que se van a emplear en el mismo y método de análisis 未知
公开(公告)号：ES2649147T3
公开(公告)日：2018-01-10
申请号：ES13893016
申请日：2013-09-09
申请人： SHIMADZU CORP
发明人： SHIMADA TAKASHI , FUKAO NORIKO , AOKI CHIKAGE , SATO TAKA-AKI
IPC分类号： C07K1/12
摘要： Un método para preparar fragmentos de péptidos, que comprende: una etapa de inmovilización del sustrato, para inmovilizar una proteína sustrato que se va proteolizar en los poros de un cuerpo poroso; y una etapa de proteolisis, para proteolizar la proteína sustrato con una proteasa poniendo en contacto entre sí el cuerpo poroso que presenta la proteína sustrato inmovilizada sobre él y micropartículas que presentan la proteasa inmovilizada sobre su superficie, en un líquido, en el que el diámetro promedio de partícula de las micropartículas es mayor que el diámetro promedio de poro del cuerpo poroso.

10. 发明专利

AU2007300996A1 Method for deparaffinization of paraffin-embedded specimen and method for analysis of paraffin-embedded specimen 未知
公开(公告)号：AU2007300996A1
公开(公告)日：2008-04-03
申请号：AU2007300996
申请日：2007-09-21
申请人： SHIMADZU CORP
发明人： AOKI YUTAKA , SHIMADA TAKASHI , SATO TAKA-AKI , TOYAMA ATSUHIKO
IPC分类号： G01N1/28 , G01N1/30 , G01N27/62 , G01N33/48
摘要： The present invention provides a method for deparaffinization useful for mass spectrometric imaging from a paraffin-embedded specimen. A method for deparaffinization of paraffin-embedded specimen comprising the steps of: exposing a biological sample by subjecting a specimen in which the biological sample is embedded in paraffin, to an organic solvent that is compatible with the paraffin under heating condition, to make the paraffin be melted and dissolved in the organic solvent; and removing the paraffin from the specimen by separating the organic solvent dissolving the paraffin, from the exposed biological sample.

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