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1. 发明申请

WO2004111244A3 METHODS FOR THE PRODUCTION OF INSULIN IN PLANTS 审中-公开
标题翻译：在植物中生产胰岛素的方法
公开(公告)号：WO2004111244A3
公开(公告)日：2005-02-03
申请号：PCT/CA2004000896
申请日：2004-06-17
申请人： SEMBIOSYS GENETICS INC , MOLONEY MAURICE M , BOOTHE JOSEPH , KEON RICHARD , NYKIFORUK CORY , VAN ROOIJEN GIJS
发明人： MOLONEY MAURICE M , BOOTHE JOSEPH , KEON RICHARD , NYKIFORUK CORY , VAN ROOIJEN GIJS
IPC分类号： A01H1/00 , C07K14/62 , C12N15/82 , A01H5/00
CPC分类号： C07K14/62 , C12N15/8257
摘要： Methods for the production of insulin in plants are described. In one embodiment, the present invention provides a method for the expression of insulin in plants comprising: (a) providing a chimeric nucleic acid construct comprising in the 5' supl; to 3' direction of transcription as operably linked components: (i) a nucleic acid sequence capable of controlling expression in plant seed cells; and (ii) a nucleic acid sequence encoding an insulin polypeptide; (b) introducing the chimeric nucleic acid construct into a plant cell; and (c) growing the plant cell into a mature plant capable of setting seed wherein the seed expresses insulin.
摘要翻译：描述了在植物中产生胰岛素的方法。在一个实施方案中，本发明提供了一种用于在植物中表达胰岛素的方法，包括：（a）提供嵌合的核酸构建体，其包含在5' 至3'转录方向作为可操作连接的组分：（i）能够控制植物种子细胞中表达的核酸序列; 和（ii）编码胰岛素多肽的核酸序列; （b）将嵌合核酸构建体引入植物细胞; 和（c）将植物细胞生长成能种植种子的成熟植物，其中种子表达胰岛素。

2. 发明专利

JP2011200251A Method for production of insulin in plant 审中-公开
标题翻译：在植物中生产胰岛素的方法
公开(公告)号：JP2011200251A
公开(公告)日：2011-10-13
申请号：JP2011124794
申请日：2011-06-03
申请人： Sembiosys Genetics Inc , セムバイオシスジェネティクスインコーポレイテッド
发明人： MOLONEY MAURICE M , BOOTHE JOSEPH , KEON RICHARD , NYKIFORUK CORY , VAN ROOIJEN GIJS
IPC分类号： C12N15/09 , A01H1/00 , A01H5/00 , C07K14/62 , C12N15/82 , C12P21/00
CPC分类号： C07K14/62 , C12N15/8257
摘要： PROBLEM TO BE SOLVED: To provide a method for the production of insulin in plants.SOLUTION: The method for the expression of insulin in plants comprises steps of: (a) providing a chimeric nucleic acid construct comprising in the 5' to 3' direction of transcription as operably linked components (i) a nucleic acid sequence capable of controlling expression in plant seed cells, and (ii) a nucleic acid sequence encoding an insulin polypeptide; (b) introducing the chimeric nucleic acid construct into a plant cell; and (c) growing the plant cell into a mature plant capable of setting seed wherein the seed expresses insulin.
摘要翻译：要解决的问题：提供在植物体内产生胰岛素的方法。解决方案：在植物中表达胰岛素的方法包括以下步骤：（a）提供包含在5'至3'方向上的嵌合核酸构建体的转录作为可操作地连接的组分（i）能够控制植物种子细胞中表达的核酸序列，和（ii）编码胰岛素多肽的核酸序列; （b）将嵌合核酸构建体引入植物细胞; 和（c）将植物细胞生长成能种植种子的成熟植物，其中种子表达胰岛素。

3. 发明专利

AU2004247762B2 Methods for the production of insulin in plants 未知
公开(公告)号：AU2004247762B2
公开(公告)日：2010-05-27
申请号：AU2004247762
申请日：2004-06-17
申请人： SEMBIOSYS GENETICS INC
发明人： BOOTHE JOSEPH , NYKIFORUK CORY , MOLONEY MAURICE M , KEON RICHARD , ROOIJEN GIJS VAN
IPC分类号： C07K14/62 , A01H1/00 , C12N15/82
摘要： Methods for the production of insulin in plants are described. In one embodiment, the present invention provides a method for the expression of insulin in plants comprising: (a) providing a chimeric nucleic acid construct comprising in the 5' to 3' direction of transcription as operably linked components: (i) a nucleic acid sequence capable of controlling expression in plant seed cells; and (ii) a nucleic acid sequence encoding an insulin polypeptide; (b) introducing the chimeric nucleic acid construct into a plant cell; and (c) growing the plant cell into a mature plant capable of setting seed wherein the seed expresses insulin.

4. 发明专利

AT405660T 未知
公开(公告)号：AT405660T
公开(公告)日：2008-09-15
申请号：AT04737835
申请日：2004-06-17
申请人： SEMBIOSYS GENETICS INC
发明人： MOLONEY MAURICE , BOOTHE JOSEPH , KEON RICHARD , NYKIFORUK CORY , VAN ROOIJEN GIJS
IPC分类号： C12N15/82 , A01H1/00 , A01H5/00 , C07K14/62
摘要： Methods for the production of insulin in plants are described. In one embodiment, the present invention provides a method for the expression of insulin in plants comprising: (a) providing a chimeric nucleic acid construct comprising in the 5' to 3' direction of transcription as operably linked components: (i) a nucleic acid sequence capable of controlling expression in plant seed cells; and (ii) a nucleic acid sequence encoding an insulin polypeptide; (b) introducing the chimeric nucleic acid construct into a plant cell; and (c) growing the plant cell into a mature plant capable of setting seed wherein the seed expresses insulin.

5. 发明专利

AR044803A1 METODOS PARA LA PRODUCCION DE INSULINA 未知
公开(公告)号：AR044803A1
公开(公告)日：2005-10-05
申请号：ARP040102114
申请日：2004-06-17
申请人： SEMBIOSYS GENETICS INC
发明人： MOLONEY MAURICE , BOOTHE JOSEPH , KEON RICHARD , NYKIFORUK CORY , VAN ROOIJEN GIJS
IPC分类号： A01H1/00 , C07K14/62 , C12N15/82 , C12N15/17 , C12N15/90 , A01H5/00 , A01H5/10
摘要： Reivindicación 1: Un método para la expresión de insulina en semillas vegetales, caracterizado porque comprende: a) la provisión de una construcción quimérica de ácido nucleico que comprende, como componentes operativamente ligados en la dirección de transcripción 5' a 3': i) una secuencia de ácido nucleico con la capacidad de controlar la expresión en células de semillas vegetales; y ii) una secuencia de ácido nucleico que codifica para un polipéptido insulínico; b) la introducción de la construcción quimérica de ácido nucleico dentro de una célula vegetal; y c) la multiplicación de la célula vegetal dentro de una planta madura con la capacidad de producir simiente, en donde la simiente expresa insulina. Reivindicación 28: Una planta con la capacidad de producir semillas que comprenden una secuencia quimérica de ácido nucleico caracterizado porque comprende, en la dirección de transcripción 5' a 3': a) una primera secuencia de ácido nucleico con la capacidad de controlar la expresión en una célula de semilla vegetal a la que está operativamente ligada; b) una segura secuencia de ácido nucleico codificadora de un polipéptido insulínico, en donde la semilla contiene insulina. Reivindicación 31: Una semilla vegetal que comprende una secuencia quimérica de ácido nucleico caracterizado porque comprende, en la dirección de transcripción 5' a 3': a) una primera secuencia de ácido nucleico con la capacidad de controlar la expresión en una célula de semilla vegetal a la que está operativamente ligada; b) una segunda secuencia de ácido nucleico que codifica para un polipéptido insulínico. Reivindicación 33: Una secuencia de ácido nucleico codificadora de insulina, ligada a una secuencia de ácido nucleico caracterizado porque comprende un promotor con la capacidad de controlar la expresión en una célula de semilla vegetal.

6. 发明专利

AT400661T 未知
公开(公告)号：AT400661T
公开(公告)日：2008-07-15
申请号：AT00954228
申请日：2000-08-23
申请人： SEMBIOSYS GENETICS INC
发明人： VAN ROOIJEN GIJS , KEON RICHARD , BOOTHE JOSEPH , SHEN YIN
IPC分类号： C12N15/82 , A01H5/00 , C12N5/10 , C12N9/64 , C12N15/12 , C12P21/02

7. 发明专利

NO20055885L 未知
公开(公告)号：NO20055885L
公开(公告)日：2006-01-17
申请号：NO20055885
申请日：2005-12-12
申请人： SEMBIOSYS GENETICS INC
发明人： MOLONEY MAURICE M , ROOIJEN GIJS VAN , BOOTHE JOSEPH , KEON RICHARD , NYKIFORUK CORY
IPC分类号： C12N15/82 , A01H1/00 , C07K14/62 , C12N15/17
摘要： Methods for the production of insulin in plants are described. In one embodiment, the present invention provides a method for the expression of insulin in plants comprising: (a) providing a chimeric nucleic acid construct comprising in the 5' to 3' direction of transcription as operably linked components: (i) a nucleic acid sequence capable of controlling expression in plant seed cells; and (ii) a nucleic acid sequence encoding an insulin polypeptide; (b) introducing the chimeric nucleic acid construct into a plant cell; and (c) growing the plant cell into a mature plant capable of setting seed wherein the seed expresses insulin.

8. 发明专利

AU2004247762A1 Methods for the production of insulin in plants 未知
公开(公告)号：AU2004247762A1
公开(公告)日：2004-12-23
申请号：AU2004247762
申请日：2004-06-17
申请人： SEMBIOSYS GENETICS INC
发明人： BOOTHE JOSEPH , NYKIFORUK CORY , MOLONEY MAURICE M , KEON RICHARD , ROOIJEN GIJS VAN
IPC分类号： C12N15/82 , A01H1/00 , A01H5/00 , C07K14/62
摘要： Methods for the production of insulin in plants are described. In one embodiment, the present invention provides a method for the expression of insulin in plants comprising: (a) providing a chimeric nucleic acid construct comprising in the 5' to 3' direction of transcription as operably linked components: (i) a nucleic acid sequence capable of controlling expression in plant seed cells; and (ii) a nucleic acid sequence encoding an insulin polypeptide; (b) introducing the chimeric nucleic acid construct into a plant cell; and (c) growing the plant cell into a mature plant capable of setting seed wherein the seed expresses insulin.

9. 发明专利

EA014887B1 СПОСОБ ЭКСПРЕССИИ ИНСУЛИНА В СЕМЕНАХ РАСТЕНИЯ, СПОСОБ ПОЛУЧЕНИЯ СЕМЯН РАСТЕНИЙ, СОДЕРЖАЩИХ ИНСУЛИН, И РАСТЕНИЯ, СПОСОБНЫЕ ПРОИЗВОДИТЬ СЕМЕНА, СОДЕРЖАЩИЕ ИНСУЛИН 未知
公开(公告)号：EA014887B1
公开(公告)日：2011-02-28
申请号：EA200600050
申请日：2004-06-17
申请人： SEMBIOSYS GENETICS INC
发明人： MOLONEY MAURICE M , BOOTHE JOSEPH , KEON RICHARD , NYKIFORUK CORY , VAN ROOIJEN GIJS
IPC分类号： C12N15/82 , A01H1/00 , A01H5/00 , C07K14/62
摘要： Описаныспособыполученияинсулинав растениях. Водномвариантеданноеизобретениеотноситсяк способуэкспрессииинсулинав растениях, включающему: (а) получениеконструкциихимернойнуклеиновойкислоты, содержащейсяв 5'-3' направлениитранскрипциив видефункциональносвязанныхкомпонентов: (i) последовательностинуклеиновойкислоты, способнойконтролироватьэкспрессиюв клеткахсемянрастений; и (ii) последовательностинуклеиновойкислоты, кодирующейполипептидинсулина; (b) введениеконструкциихимернойнуклеиновойкислотыв клеткурастенияи (с) выращиваниеклеткирастенийв зреломрастении, способномдаватьсемена, гдесеменаэкспрессируютинсулин.

10. 发明专利

PT1633876E MÉTODOS PARA A PRODUÇÃO DE INSULINA EM PLANTAS 未知
公开(公告)号：PT1633876E
公开(公告)日：2008-11-03
申请号：PT04737835
申请日：2004-06-17
申请人： SEMBIOSYS GENETICS INC
发明人： ROOIJEN GIJS VAN , MOLONEY MAURICE M , BOOTHE JOSEPH , KEON RICHARD , NYKIFORUK CORY
IPC分类号： C12N15/82 , A01H1/00 , A01H5/00 , C07K14/62

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