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1. 发明公开

EP0457214A3 Method and reagent composition for the determination of alanine aminotransferase and HBsAg antigen in the same biological specimen 失效
标题翻译：用于确定相同生物样本中的氨苄青霉素和HBSAG抗原的方法和试剂组合物
公开(公告)号：EP0457214A3
公开(公告)日：1993-05-05
申请号：EP91107627.1
申请日：1991-05-10
申请人： SCLAVO S.p.A.
发明人： Fabrizi, Paolo , Donnini, Francesco , Tabacco, Alessandro , Tarli, Paolo
IPC分类号： G01N33/573 , G01N33/576 , G01N33/577 , C12Q1/52
CPC分类号： G01N33/573 , G01N33/5764 , Y10S435/81 , Y10S436/82
摘要： The enzyme alanine aminotransferase (ALT) is colorimetrically determined as the hydrogen peroxide obtained in the pyruvate hydrolysis reaction catalysed by the enzyme pyruvate oxidase which develops a colour read at 550 nm making use of a reagent composition buffered at pH comprised within 7.0 and 7.5 containing L-alanine, ketoglutaric acid, a source of inorganic phosphorous, the enzyme pyruvate oxidase, a system for revealing the hydrogen peroxide and optionally one or more co-factors which interact in the enzymatic reaction catalysed by the enzyme pyruvate oxidase. It is moreover described a method for the determination of the enzyme alanine aminotransferase making use of said reagent composition and of the surface antigen of the hepatitis B virus (HBsAg), by enzymeimmune assay, in the same serum specimen and in the same well of a microtitration plate. The method is particularly useful for checking the suitability of the blood intended for transfusions since it can be easily carried out with equipments and instruments widely used for the immunoenzymatic screening of infectivity. Furthermore it enables to test in the same analytical assay the two labels of hepatocyte infections necessarily researched in the transfusion centres on biological specimens and to select among the donors the most likely candidates to transmission of the NANB hepatitis virus.

2. 发明公开

EP0468180A3 Buffer solutions containing collagenase, their preparation and use for diluting human sera 失效
标题翻译：包含胶原蛋白的缓冲溶液，其制备和用于稀释人类血清
公开(公告)号：EP0468180A3
公开(公告)日：1993-05-12
申请号：EP91109495.1
申请日：1991-06-10
申请人： SCLAVO S.p.A.
发明人： Fabrizi, Paolo , Donnini, Francesco
IPC分类号： G01N33/53 , G01N33/573 , G01N33/569 , G01N33/564
CPC分类号： G01N33/564 , G01N33/5306 , G01N33/569 , G01N33/573
摘要： A human sera dilution buffer solution containing the enzyme collagenase is described, for use in preventing false positive indication reactions taking place when specific IgMs are sought in human sera by ELISA capture assays, in which the enzymatic conjugate consists of antibodies bound to ALP with glutaraldehyde.

3. 发明公开

EP0468180A2 Buffer solutions containing collagenase, their preparation and use for diluting human sera 失效
标题翻译：含胶原酶的缓冲溶液，其穿衣和使用人血清的稀释。
公开(公告)号：EP0468180A2
公开(公告)日：1992-01-29
申请号：EP91109495.1
申请日：1991-06-10
申请人： SCLAVO S.p.A.
发明人： Fabrizi, Paolo , Donnini, Francesco
IPC分类号： G01N33/53 , G01N33/573 , G01N33/569 , G01N33/564
CPC分类号： G01N33/564 , G01N33/5306 , G01N33/569 , G01N33/573
摘要： A human sera dilution buffer solution containing the enzyme collagenase is described, for use in preventing false positive indication reactions taking place when specific IgMs are sought in human sera by ELISA capture assays, in which the enzymatic conjugate consists of antibodies bound to ALP with glutaraldehyde.
摘要翻译：含有酶胶原酶甲人血清稀释缓冲液溶液中防止假阳性指示反应发生当特异性IgM的人血清中通过ELISA捕获分析寻求描述于用途，其中酶偶联物结合至ALP用戊二醛抗体besteht。

4. 发明公开

EP0457214A2 Method and reagent composition for the determination of alanine aminotransferase and HBsAg antigen in the same biological specimen 失效
标题翻译：方法和试剂为丙氨酸氨基转移酶和HBsAg抗原的相同生物样品中的确定。
公开(公告)号：EP0457214A2
公开(公告)日：1991-11-21
申请号：EP91107627.1
申请日：1991-05-10
申请人： SCLAVO S.p.A.
发明人： Fabrizi, Paolo , Donnini, Francesco , Tabacco, Alessandro , Tarli, Paolo
IPC分类号： G01N33/573 , G01N33/576 , G01N33/577 , C12Q1/52
CPC分类号： G01N33/573 , G01N33/5764 , Y10S435/81 , Y10S436/82
摘要： The enzyme alanine aminotransferase (ALT) is colorimetrically determined as the hydrogen peroxide obtained in the pyruvate hydrolysis reaction catalysed by the enzyme pyruvate oxidase which develops a colour read at 550 nm making use of a reagent composition buffered at pH comprised within 7.0 and 7.5 containing L-alanine, ketoglutaric acid, a source of inorganic phosphorous, the enzyme pyruvate oxidase, a system for revealing the hydrogen peroxide and optionally one or more co-factors which interact in the enzymatic reaction catalysed by the enzyme pyruvate oxidase. It is moreover described a method for the determination of the enzyme alanine aminotransferase making use of said reagent composition and of the surface antigen of the hepatitis B virus (HBsAg), by enzymeimmune assay, in the same serum specimen and in the same well of a microtitration plate. The method is particularly useful for checking the suitability of the blood intended for transfusions since it can be easily carried out with equipments and instruments widely used for the immunoenzymatic screening of infectivity. Furthermore it enables to test in the same analytical assay the two labels of hepatocyte infections necessarily researched in the transfusion centres on biological specimens and to select among the donors the most likely candidates to transmission of the NANB hepatitis virus.
摘要翻译：酶丙氨酸转氨酶（ALT）是比色确定性开采如通过酶丙酮酸氧化酶催化的丙酮酸盐的水解反应获得的过氧化氢开发出在550nm处利用在pH 7.0和7.5含有L-内包含缓冲试剂组合物的读取彩色丙氨酸，酮戊二酸，无机磷源，酶丙酮酸氧化酶，为揭示的过氧化氢和任选的一种或多种辅因子中哪些被酶丙酮酸氧化酶催化的酶反应进行交互的系统。它更经描述的方法用于酶丙氨酸转氨酶利用所述试剂组合物，并通过酶免疫测定中的乙型肝炎病毒（HBsAg）的表面抗原的测定，在相同的血清样品并在一个相同的井微滴板。该方法是用于检查旨在用于输血，因为它可以与广泛用于感染的免疫酶的筛选设备和仪器可以容易地进行血液的适用性是特别有用的。进一步使IT人员能够在同一个分析测定肝感染的两个标签中的生物标本输血中心必然的研究和最有可能的候选来NANB肝炎病毒传播的捐助者选择进行测试。

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